DrLLPS - Data resource of LLPS

LLPS-Sac-0060
MRT4

▼ OVERVIEW


Status:	Reviewed
Protein Name:	Ribosome assembly factor MRT4; mRNA turnover protein 4
Gene Name:	MRT4, YKL009W, YKL160
Ensembl Gene:	YKL009W
Ensembl Protein:	YKL009W_mRNA
Organism:	Saccharomyces cerevisiae
Taxa ID:	559292
LLPS Type:	Client

PDB: 6EM5 (2, 6, L, M, N, Q, R, S, T, U, Z, c, d, e, f, g, i, j, k, E, G, O, V, X, Y, h, B, C, H, A, K, m, D, W, l, b, o, n, r, s, t, y, z, p, q, u, v, w, I, J, x, 3, 4, 5, 1, F, P) More

▼ PROPERTY

——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:

Condensate	Description	Tissue/Cell	PMIDs
Nucleolus	"...Out of the 142 yeast nucleolar proteins that have at least one human homologue, 124 are found in the updated nucleolar proteome."	Saccharomyces cerevisiae	15635413
Stress granule	"...Here we systematically identified proteins sequestered in Saccharomyces cerevisiae at 46 °C by a SG co‐localization screen and proteomic analysis of insoluble protein fractions. We identified novel SG components including essential aminoacyl‐tRNA synthetases."	Saccharomyces cerevisiae	26484595
Stress granule	"...Here we systematically identified proteins sequestered in Saccharomyces cerevisiae at 46 °C by a SG co‐localization screen and proteomic analysis of insoluble protein fractions."	Saccharomyces cerevisiae	26484595
Others	"...We have carried out extensive proteomic studies to quantify heat-triggered aggregation and subsequent disaggregation in budding yeast, identifying more than 170 endogenous proteins aggregating within minutes of heat shock in multiple subcellular compartments."	Saccharomyces cerevisiae	26359986

▼ FUNCTION

Component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus, and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes.

▼ CROSS REFERENCE

Database	Nucleotide ID	Protein ID
Ensembl	YKL009W_mRNA	YKL009W_mRNA
UniProt		P33201, MRT4_YEAST, D6VXS7
GeneBank	BK006944, S59773, X61398, S53418, Z28009	DAA09147.1, AAC60552.1, AAB24904.1, CAA81844.1
RefSeq	NM_001179575.1	NP_012916.1
Entrez	853860

▼ SEQUENCE

Protein Sequence (FASTA)
1 MPRSKRSKLV TLAQTDKKGR ENKERIFDEV REALDTYRYV WVLHLDDVRT PVLQEIRTSW 60 61 AGSKLIMGKR KVLQKALGEK REEEYKENLY QLSKLCSGVT GLLFTDEDVN TVKEYFKSYV 120 121 RSDYSRPNTK APLTFTIPEG IVYSRGGQIP AEEDVPMIHS LEPTMRNKFE IPTKIKAGKI 180 181 TIDSPYLVCT EGEKLDVRQA LILKQFGIAA SEFKVKVSAY YDNDSSTVES TNINME 236
Nucleotide CDS Sequence (FASTA)
1 ATGCCAAGAT CAAAACGTTC CAAGCTAGTC ACTTTAGCAC AAACCGATAA AAAGGGCAGA 60 61 GAAAATAAAG AAAGAATTTT CGATGAAGTG AGGGAAGCCT TAGATACTTA TAGATACGTC 120 121 TGGGTCCTAC ATCTTGACGA CGTAAGAACT CCAGTTTTAC AAGAAATCAG AACATCTTGG 180 181 GCAGGCTCTA AGTTAATTAT GGGTAAGAGG AAAGTTTTAC AAAAAGCATT GGGAGAAAAG 240 241 AGGGAAGAAG AATACAAAGA AAATCTGTAT CAATTGAGTA AGCTTTGTAG CGGTGTCACT 300 301 GGTTTATTGT TCACTGATGA GGATGTCAAC ACTGTCAAGG AATACTTTAA GTCATACGTT 360 361 CGTTCAGACT ATTCAAGACC GAACACGAAA GCACCACTAA CATTTACAAT TCCTGAGGGC 420 421 ATTGTCTACT CACGTGGTGG TCAAATTCCA GCTGAGGAAG ATGTTCCAAT GATTCATTCT 480 481 TTAGAGCCAA CTATGAGAAA CAAATTTGAA ATTCCAACTA AAATCAAAGC TGGTAAGATC 540 541 ACCATTGACA GTCCATATTT AGTTTGCACT GAAGGAGAAA AATTAGATGT TCGTCAAGCT 600 601 TTAATACTGA AACAATTCGG TATCGCTGCT TCTGAATTCA AAGTCAAGGT GTCGGCCTAC 660 661 TATGACAACG ATAGCTCCAC TGTTGAAAGC ACTAACATCA ACATGGAATA A 711

▼ KEYWORD

ID	Family
KW-0002	3D-structure
KW-0181	Complete proteome
KW-0963	Cytoplasm
KW-0539	Nucleus
KW-1185	Reference proteome
KW-0690	Ribosome biogenesis

▼ GENE ONTOLOGY

ID	Classification	Description
GO:0005737	Cellular Component	Cytoplasm
GO:0005730	Cellular Component	Nucleolus
GO:0005654	Cellular Component	Nucleoplasm
GO:0005634	Cellular Component	Nucleus
GO:0030687	Cellular Component	Preribosome, large subunit precursor
GO:0070180	Molecular Function	Large ribosomal subunit rRNA binding
GO:0000956	Biological Process	Nuclear-transcribed mRNA catabolic process
GO:0000027	Biological Process	Ribosomal large subunit assembly
GO:0042273	Biological Process	Ribosomal large subunit biogenesis
GO:0000055	Biological Process	Ribosomal large subunit export from nucleus
GO:0006364	Biological Process	RRNA processing

▼ KEGG

sce:YKL009W

▼ ANNOTATION

Disorder
IUPred2A

Domain
SMART	Pfam	InterPro	CDD	Gene3D

PTM
EPSD	PLMD	dbPTM	BioGRID

Interaction
HINT	Mentha	YTRP	PINA	IID
iRefIndex	MIST

Physicochemical
Compute pI/Mw	AAindex

Function
PIRSF

Structure
PDB

Localization
NLSdb

▼ ORTHOLOGY

DrLLPS ID	Organism	Identity	E-value	Score
LLPS-Asg-0767	Ashbya gossypii	72.03	1e-128	370
LLPS-Kop-0908	Komagataella pastoris	67.11	1e-113	332
LLPS-Scj-0063	Schizosaccharomyces japonicus	49.78	6e-76	236
LLPS-Scc-0290	Schizosaccharomyces cryophilus	48.03	3e-75	234
LLPS-Scp-0796	Schizosaccharomyces pombe	46.72	2e-74	233
LLPS-Yal-0137	Yarrowia lipolytica	46.29	4e-75	234
LLPS-Tum-1148	Tuber melanosporum	45.96	2e-70	222
LLPS-Phn-0030	Phaeosphaeria nodorum	44.3	5e-71	225
LLPS-Lem-0190	Leptosphaeria maculans	42.98	7e-68	216
LLPS-Pyt-0156	Pyrenophora teres	42.11	1e-67	215
LLPS-Pytr-0516	Pyrenophora triticirepentis	42.11	1e-67	215
LLPS-Ova-0501	Ovis aries	41.94	3e-20	87.8
LLPS-Abg-0251	Absidia glauca	41.52	3e-54	180
LLPS-Ast-0779	Aspergillus terreus	40.93	1e-57	190
LLPS-Blg-0497	Blumeria graminis	40.79	9e-61	197
LLPS-Zyt-0814	Zymoseptoria tritici	40.61	5e-58	191
LLPS-Beb-0133	Beauveria bassiana	40.6	5e-60	196
LLPS-Nef-0724	Neosartorya fischeri	40.43	8e-58	190
LLPS-Asfu-0216	Aspergillus fumigatus	40.43	3e-57	189
LLPS-Aso-0134	Aspergillus oryzae	40.17	1e-56	187
LLPS-Asf-0869	Aspergillus flavus	40.17	1e-56	187
LLPS-Asn-0688	Aspergillus nidulans	40.0	4e-57	188
LLPS-Cog-0357	Colletotrichum gloeosporioides	39.83	2e-58	192
LLPS-Orc-1002	Oryctolagus cuniculus	39.82	7e-49	167
LLPS-Usm-0675	Ustilago maydis	39.82	4e-45	159
LLPS-Fud-0849	Fukomys damarensis	39.74	3e-49	168
LLPS-Nec-0262	Neurospora crassa	39.74	4e-57	188
LLPS-Asc-0356	Aspergillus clavatus	39.57	3e-56	186
LLPS-Gag-1021	Gaeumannomyces graminis	39.57	2e-58	192
LLPS-Map-0508	Magnaporthe poae	39.57	4e-56	186
LLPS-Caj-1348	Callithrix jacchus	39.56	1e-48	166
LLPS-Tut-0625	Tursiops truncatus	39.38	2e-47	163
LLPS-Spr-0631	Sporisorium reilianum	39.37	8e-45	158
LLPS-Loa-0769	Loxodonta africana	39.3	3e-48	165
LLPS-Pug-0300	Puccinia graminis	39.22	3e-50	170
LLPS-Cogr-1212	Colletotrichum graminicola	39.17	5e-59	193
LLPS-Coo-0320	Colletotrichum orbiculare	39.0	7e-59	193
LLPS-Urm-2623	Ursus maritimus	38.94	1e-47	163
LLPS-Aon-2594	Aotus nancymaae	38.86	8e-49	167
LLPS-Chs-1377	Chlorocebus sabaeus	38.86	3e-48	165
LLPS-Gog-0212	Gorilla gorilla	38.86	7e-49	167
LLPS-Bot-0997	Bos taurus	38.86	3e-48	165
LLPS-Mup-0508	Mustela putorius furo	38.86	7e-49	167
LLPS-Fec-0015	Felis catus	38.86	2e-48	166
LLPS-Hos-0180	Homo sapiens	38.86	1e-48	166
LLPS-Pap-1168	Pan paniscus	38.86	5e-49	167
LLPS-Pat-2815	Pan troglodytes	38.86	1e-48	166
LLPS-Otg-1079	Otolemur garnettii	38.86	3e-48	165
LLPS-Cap-1950	Cavia porcellus	38.86	1e-48	166
LLPS-Poa-0433	Pongo abelii	38.86	1e-48	166
LLPS-Ict-1901	Ictidomys tridecemlineatus	38.86	5e-48	164
LLPS-Dos-0983	Dothistroma septosporum	38.86	9e-55	182
LLPS-Cas-0944	Carlito syrichta	38.86	1e-48	166
LLPS-Maf-1201	Macaca fascicularis	38.43	8e-48	164
LLPS-Icp-0937	Ictalurus punctatus	38.43	1e-50	171
LLPS-Myl-0507	Myotis lucifugus	38.43	1e-47	164
LLPS-Aim-0492	Ailuropoda melanoleuca	38.43	2e-47	163
LLPS-Mal-0374	Mandrillus leucophaeus	38.43	3e-48	165
LLPS-Man-1423	Macaca nemestrina	38.43	8e-48	164
LLPS-Rhb-1728	Rhinopithecus bieti	38.43	5e-48	164
LLPS-Mam-1070	Macaca mulatta	38.43	8e-48	164
LLPS-Caf-2962	Canis familiaris	38.43	1e-47	164
LLPS-Xet-0187	Xenopus tropicalis	38.43	2e-48	166
LLPS-Trv-0683	Trichoderma virens	38.33	2e-54	182
LLPS-Scf-0003	Scleropages formosus	38.14	1e-49	169
LLPS-Mao-0207	Magnaporthe oryzae	38.02	2e-55	184
LLPS-Ved-0394	Verticillium dahliae	38.02	2e-56	186
LLPS-Dio-0782	Dipodomys ordii	37.99	2e-48	166
LLPS-Mod-2992	Monodelphis domestica	37.99	5e-48	164
LLPS-Dar-1855	Danio rerio	37.99	1e-49	169
LLPS-Eqc-0501	Equus caballus	37.99	2e-47	163
LLPS-Mum-0544	Mus musculus	37.87	3e-46	160
LLPS-Mea-1032	Mesocricetus auratus	37.87	5e-49	167
LLPS-Orn-0512	Oreochromis niloticus	37.83	4e-50	170
LLPS-Asm-0545	Astyanax mexicanus	37.73	4e-46	160
LLPS-Mel-1010	Melampsora laricipopulina	37.72	1e-41	148
LLPS-Fuv-0918	Fusarium verticillioides	37.5	4e-53	177
LLPS-Fuo-0458	Fusarium oxysporum	37.5	7e-55	182
LLPS-Pof-0506	Poecilia formosa	37.39	4e-50	170
LLPS-Trr-0847	Trichoderma reesei	37.08	4e-52	176
LLPS-Fus-0226	Fusarium solani	36.97	1e-54	182
LLPS-Orl-1269	Oryzias latipes	36.96	2e-48	166
LLPS-Chr-0333	Chlamydomonas reinhardtii	36.94	5e-41	147
LLPS-Put-0407	Puccinia triticina	36.89	2e-46	160
LLPS-Sah-1542	Sarcophilus harrisii	36.82	8e-43	151
LLPS-Ran-0392	Rattus norvegicus	36.7	6e-33	124
LLPS-Ora-0003	Ornithorhynchus anatinus	36.56	2e-45	158
LLPS-Gaa-0982	Gasterosteus aculeatus	36.52	6e-49	167
LLPS-Lac-0254	Latimeria chalumnae	36.28	7e-46	159
LLPS-Pes-1549	Pelodiscus sinensis	36.26	1e-31	120
LLPS-Asni-0217	Aspergillus niger	36.17	5e-47	162
LLPS-Tar-2638	Takifugu rubripes	36.09	1e-46	161
LLPS-Ten-0227	Tetraodon nigroviridis	36.09	3e-46	160
LLPS-Drm-1649	Drosophila melanogaster	36.04	3e-36	134
LLPS-Paa-2571	Papio anubis	35.87	5e-42	149
LLPS-Meg-0637	Meleagris gallopavo	35.84	6e-44	154
LLPS-Leo-1142	Lepisosteus oculatus	35.81	5e-47	162
LLPS-Fia-0421	Ficedula albicollis	35.81	1e-44	155
LLPS-Gaga-3258	Gallus gallus	35.81	7e-45	156
LLPS-Art-0182	Arabidopsis thaliana	35.78	2e-36	134
LLPS-Scm-0022	Scophthalmus maximus	35.65	1e-47	164
LLPS-Via-0071	Vigna angularis	35.61	2e-20	90.1
LLPS-Nol-1209	Nomascus leucogenys	35.43	1e-36	134
LLPS-Orp-0959	Oryza punctata	34.91	9e-12	64.7
LLPS-Miv-0199	Microbotryum violaceum	34.88	2e-45	157
LLPS-Cea-0480	Cercocebus atys	34.84	3e-33	125
LLPS-Sus-0155	Sus scrofa	33.85	4e-40	145
LLPS-Tag-0648	Taeniopygia guttata	33.85	2e-32	122
LLPS-Brr-2166	Brassica rapa	33.63	3e-33	125
LLPS-Cis-0248	Ciona savignyi	33.62	5e-37	137
LLPS-Brn-0031	Brassica napus	33.49	2e-33	126
LLPS-Bro-1044	Brassica oleracea	33.49	4e-33	125
LLPS-Crn-0159	Cryptococcus neoformans	33.47	4e-44	154
LLPS-Osl-0377	Ostreococcus lucimarinus	33.04	1e-32	124
LLPS-Php-0975	Physcomitrella patens	32.77	2e-26	107
LLPS-Amt-0057	Amborella trichopoda	32.57	1e-33	127
LLPS-Cae-0776	Caenorhabditis elegans	32.29	1e-27	110
LLPS-Dac-0576	Daucus carota	32.11	4e-30	117
LLPS-Pot-2679	Populus trichocarpa	32.11	4e-29	115
LLPS-Prp-1260	Prunus persica	32.11	1e-30	119
LLPS-Coc-0601	Corchorus capsularis	32.11	3e-32	123
LLPS-Gor-0836	Gossypium raimondii	31.65	4e-25	103
LLPS-Cii-0010	Ciona intestinalis	31.46	1e-22	97.4
LLPS-Thc-0386	Theobroma cacao	31.19	3e-31	120
LLPS-Orm-1543	Oryza meridionalis	31.13	8e-15	78.2
LLPS-Sol-0257	Solanum lycopersicum	31.11	8e-25	103
LLPS-Glm-1690	Glycine max	31.11	2e-31	120
LLPS-Met-0286	Medicago truncatula	31.11	1e-30	119
LLPS-Sem-0939	Selaginella moellendorffii	31.0	8e-31	119
LLPS-Mae-0672	Manihot esculenta	30.73	3e-29	115
LLPS-Zem-0953	Zea mays	30.71	1e-27	111
LLPS-Sot-0334	Solanum tuberosum	30.67	4e-25	104
LLPS-Viv-0130	Vitis vinifera	30.67	9e-30	116
LLPS-Sei-0193	Setaria italica	30.43	9e-29	114
LLPS-Hea-0666	Helianthus annuus	30.28	2e-28	112
LLPS-Org-0068	Oryza glaberrima	30.24	3e-24	102
LLPS-Phv-0771	Phaseolus vulgaris	30.22	2e-29	115
LLPS-Sob-0405	Sorghum bicolor	30.0	5e-29	114
LLPS-Orbr-0617	Oryza brachyantha	30.0	3e-29	115
LLPS-Nia-0816	Nicotiana attenuata	29.78	1e-24	102
LLPS-Mua-0575	Musa acuminata	29.73	5e-21	92.8
LLPS-Brd-0984	Brachypodium distachyon	29.57	2e-26	107
LLPS-Cym-0105	Cyanidioschyzon merolae	29.46	2e-24	102
LLPS-Tra-0769	Triticum aestivum	29.2	5e-27	109
LLPS-Orb-0356	Oryza barthii	28.85	1e-22	98.2
LLPS-Lep-0442	Leersia perrieri	28.76	6e-26	106
LLPS-Ori-1411	Oryza indica	28.26	8e-27	108
LLPS-Orni-0293	Oryza nivara	28.26	8e-27	108
LLPS-Ors-0020	Oryza sativa	28.26	8e-27	108
LLPS-Chc-0354	Chondrus crispus	28.16	7e-21	91.3
LLPS-Cus-1934	Cucumis sativus	28.12	1e-17	82.8
LLPS-Hov-0376	Hordeum vulgare	28.05	8e-23	97.8
LLPS-Vir-1486	Vigna radiata	27.44	2e-13	72.0
LLPS-Orgl-0268	Oryza glumaepatula	27.35	9e-24	100
LLPS-Gas-0408	Galdieria sulphuraria	27.23	4e-26	106
LLPS-Anc-2214	Anolis carolinensis	25.49	2e-07	54.7
LLPS-Arl-2653	Arabidopsis lyrata	25.23	5e-12	68.2
LLPS-Orr-0367	Oryza rufipogon	25.23	5e-09	59.3
LLPS-Scs-0609	Sclerotinia sclerotiorum	24.64	1e-08	58.2

LLPS-Sac-0060MRT4

Protein Sequence

Nucleotide Sequence

LLPS-Sac-0060
MRT4