• Disorder
  • Domain
  • PTM
  • Variation
  • Mutation
  • Interaction
  • Disease
  • Drug
  • Physicochemical
  • Function
  • Proteomics
  • Structure
  • Localization
  • Expression
  • Element
  • Methylation

LLPS-Tra-1921
FEN1

Integrated Annotations

▼ OVERVIEW


Status: Unreviewed
Protein Name: Flap endonuclease 1
Gene Name: FEN1
Ensembl Gene: TraesCS1B02G377800
Ensembl Protein: TraesCS1B02G377800.1
Organism: Triticum aestivum
Taxa ID: 4565
LLPS Type: LLPS regulator


▼ PROPERTY



——— Disorder propensity (calculated by IUPred2A)

▼ Classification


Condensates:
CondensateEvidenceOrthologs
Nucleolus, P-bodyPredicted from orthologs(View)

▼ FUNCTION


Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

▼ CROSS REFERENCE


DatabaseNucleotide IDProtein ID
EnsemblTraesCS1B02G377800.1TraesCS1B02G377800.1
UniProtA0A3B5Z3P0, A0A3B5Z3P0_WHEAT

▼ SEQUENCE


Protein Sequence (FASTA)
1     MGVKGLTKLL  ADNAPKSMRE  QKFESYFGRR  IAVDASMCIY  QFLIVVGRTG  METLTNEAGD  60
61    ITSHLQGMFS  RTIRLLEAGI  KPVYVFDGKP  PEMKKDELLK  RHAKRNEATE  ELTKAVEAGD  120
121   TDAIEKFSKR  TVKVTKQHND  DCKRLLRLMG  VPVVEAPCEA  ESQCAALCKN  DKVYAVASED  180
181   MDSLTFGATR  FVRHLMDPSS  RKIPVMEFEV  AKILEELEFT  MDQFIDLCIL  CGCDYCDSIK  240
241   GIGGLTALKL  IRQHGSIEGI  LENINKDKYQ  IPEDWPYQEA  RRMFKEPDVT  LDIPELKWTA  300
301   PDEEGLVNFL  VKENGFNQDR  VTKAIEKIKS  AKNKSSQGRL  ESFFKPTVST  SVPLKRKETA  360
361   EKPASAAASK  KTKSTRGRKK  380
Nucleotide CDS Sequence (FASTA)
1     ATGGGGGTCA  AGGGTTTGAC  GAAGCTACTG  GCGGACAACG  CGCCCAAGTC  GATGAGGGAG  60
61    CAGAAGTTCG  AGAGCTACTT  CGGCCGCCGC  ATCGCCGTCG  ACGCCAGCAT  GTGCATCTAC  120
121   CAGTTCCTCA  TTGTAGTTGG  AAGGACAGGG  ATGGAAACCC  TTACAAACGA  AGCCGGTGAT  180
181   ATCACCAGTC  ATTTGCAAGG  CATGTTCAGC  CGGACAATAA  GGTTGCTCGA  GGCAGGAATT  240
241   AAACCAGTAT  ATGTTTTTGA  TGGCAAGCCT  CCTGAAATGA  AGAAGGACGA  GCTTTTAAAA  300
301   AGACACGCAA  AGAGGAATGA  AGCAACAGAA  GAGCTGACGA  AGGCAGTAGA  GGCAGGAGAT  360
361   ACGGATGCAA  TTGAAAAATT  CAGCAAGAGA  ACTGTAAAGG  TCACGAAGCA  GCACAATGAT  420
421   GATTGTAAGC  GTCTACTAAG  ACTGATGGGT  GTTCCTGTTG  TGGAGGCTCC  TTGTGAAGCA  480
481   GAATCACAAT  GTGCTGCCCT  TTGCAAGAAT  GACAAGGTGT  ATGCTGTTGC  ATCCGAAGAT  540
541   ATGGACTCAC  TTACTTTTGG  AGCTACACGG  TTTGTTCGTC  ATTTGATGGA  TCCAAGTTCC  600
601   AGGAAAATAC  CTGTTATGGA  ATTTGAAGTT  GCGAAAATTC  TTGAGGAGCT  TGAATTCACT  660
661   ATGGACCAAT  TCATTGACTT  GTGCATCCTC  TGTGGATGTG  ACTACTGTGA  TAGCATTAAA  720
721   GGCATTGGTG  GTCTTACAGC  TCTGAAGCTC  ATTCGTCAGC  ACGGGTCCAT  TGAGGGCATT  780
781   CTGGAAAATA  TTAATAAAGA  CAAATATCAA  ATTCCTGAGG  ACTGGCCTTA  TCAAGAGGCT  840
841   CGGCGCATGT  TCAAGGAACC  CGATGTAACA  CTGGATATTC  CCGAGCTCAA  ATGGACTGCA  900
901   CCTGATGAGG  AGGGCCTCGT  GAACTTTCTG  GTGAAAGAAA  ATGGTTTCAA  TCAAGATCGT  960
961   GTGACAAAGG  CCATAGAGAA  GATTAAATCA  GCGAAGAATA  AATCATCCCA  AGGAAGGCTC  1020
1021  GAGTCATTTT  TCAAGCCAAC  TGTTAGCACA  TCAGTGCCAT  TGAAGCGGAA  GGAAACTGCA  1080
1081  GAGAAACCAG  CCAGTGCAGC  CGCTAGCAAG  AAGACGAAGT  CAACCCGCGG  AAGGAAGAAA  1140
1141  TAA  1143

▼ KEYWORD


ID
Family
Complete proteome
DNA damage
DNA repair
DNA replication
Endonuclease
Exonuclease
Hydrolase
Magnesium
Metal-binding
Mitochondrion
Nuclease
Nucleus
Phosphoprotein
Reference proteome

▼ GENE ONTOLOGY


ID
Classification
Description
Cellular Component
Mitochondrion
Cellular Component
Nucleolus
Cellular Component
Nucleoplasm
Molecular Function
5'-3' exonuclease activity
Molecular Function
5'-flap endonuclease activity
Molecular Function
DNA binding
Molecular Function
Magnesium ion binding
Biological Process
Base-excision repair
Biological Process
DNA replication
Biological Process
DNA replication, removal of RNA primer

▼ ORTHOLOGY


DrLLPS IDOrganismIdentityE-valueScore
LLPS-Hov-1098Hordeum vulgare98.230.0 695
LLPS-Brd-0390Brachypodium distachyon93.540.0 701
LLPS-Ori-0397Oryza indica88.760.0 667
LLPS-Ors-1482Oryza sativa88.760.0 667
LLPS-Orbr-0792Oryza brachyantha88.760.0 669
LLPS-Org-0494Oryza glaberrima88.760.0 667
LLPS-Sei-1775Setaria italica87.920.0 669
LLPS-Sob-0247Sorghum bicolor85.960.0 657
LLPS-Lep-0111Leersia perrieri84.550.0 626
LLPS-Orp-1394Oryza punctata84.270.0 619
LLPS-Zem-0587Zea mays83.560.0 654
LLPS-Mua-0298Musa acuminata82.30.0 635
LLPS-Glm-1176Glycine max81.840.0 619
LLPS-Prp-0732Prunus persica81.840.0 620
LLPS-Arl-0879Arabidopsis lyrata81.010.0 613
LLPS-Met-0011Medicago truncatula81.010.0 613
LLPS-Art-0221Arabidopsis thaliana81.010.0 609
LLPS-Orgl-1200Oryza glumaepatula80.850.0 585
LLPS-Brn-0901Brassica napus80.730.0 610
LLPS-Brr-1381Brassica rapa80.730.0 610
LLPS-Nia-0211Nicotiana attenuata80.390.0 606
LLPS-Mae-0099Manihot esculenta80.170.0 616
LLPS-Coc-1602Corchorus capsularis80.170.0 608
LLPS-Bro-2245Brassica oleracea80.170.0 610
LLPS-Via-0889Vigna angularis79.890.0 604
LLPS-Cus-0532Cucumis sativus79.890.0 607
LLPS-Pot-0330Populus trichocarpa79.890.0 606
LLPS-Viv-0328Vitis vinifera79.890.0 615
LLPS-Sot-1640Solanum tuberosum79.782e-99 301
LLPS-Hea-1035Helianthus annuus79.610.0 611
LLPS-Thc-1819Theobroma cacao79.050.0 602
LLPS-Phv-1094Phaseolus vulgaris79.050.0 597
LLPS-Amt-0816Amborella trichopoda78.770.0 598
LLPS-Sem-1082Selaginella moellendorffii77.520.0 578
LLPS-Dac-1311Daucus carota77.030.0 608
LLPS-Gor-0173Gossypium raimondii76.820.0 592
LLPS-Vir-1643Vigna radiata73.390.0 557
LLPS-Php-0223Physcomitrella patens73.370.0 555
LLPS-Sol-0104Solanum lycopersicum72.270.0 557
LLPS-Orr-1696Oryza rufipogon67.322e-134 392
LLPS-Chr-0596Chlamydomonas reinhardtii62.821e-164 474
LLPS-Osl-0082Ostreococcus lucimarinus59.724e-146 427
LLPS-Orm-1176Oryza meridionalis59.591e-137 414
LLPS-Dar-1793Danio rerio57.73e-148 432
LLPS-Xim-1841Xiphophorus maculatus57.673e-144 421
LLPS-Orl-1540Oryzias latipes57.429e-145 425
LLPS-Scm-0534Scophthalmus maximus57.397e-144 421
LLPS-Pof-0073Poecilia formosa57.32e-142 417
LLPS-Orn-3509Oreochromis niloticus57.143e-145 424
LLPS-Cis-0672Ciona savignyi56.982e-149 434
LLPS-Icp-0514Ictalurus punctatus56.861e-146 427
LLPS-Scf-2853Scleropages formosus56.862e-146 427
LLPS-Gaa-0922Gasterosteus aculeatus56.822e-142 417
LLPS-Lac-1368Latimeria chalumnae56.644e-114 342
LLPS-Asm-0753Astyanax mexicanus56.586e-144 422
LLPS-Sah-0179Sarcophilus harrisii56.033e-136 401
LLPS-Xet-0658Xenopus tropicalis55.977e-137 403
LLPS-Cii-0087Ciona intestinalis55.592e-144 422
LLPS-Orb-1513Oryza barthii55.465e-128 379
LLPS-Orni-0004Oryza nivara55.464e-123 378
LLPS-Anp-0270Anas platyrhynchos55.43e-141 414
LLPS-Pes-0376Pelodiscus sinensis55.43e-141 415
LLPS-Mod-2146Monodelphis domestica55.174e-139 409
LLPS-Meg-1791Meleagris gallopavo55.111e-140 412
LLPS-Gaga-0195Gallus gallus55.117e-141 413
LLPS-Drm-0311Drosophila melanogaster54.993e-133 394
LLPS-Tag-2635Taeniopygia guttata54.551e-134 397
LLPS-Fia-1618Ficedula albicollis54.264e-133 395
LLPS-Fud-0817Fukomys damarensis54.112e-129 384
LLPS-Aon-3219Aotus nancymaae53.823e-130 386
LLPS-Dio-2286Dipodomys ordii53.821e-132 392
LLPS-Aim-0403Ailuropoda melanoleuca53.825e-135 398
LLPS-Myl-2294Myotis lucifugus53.824e-135 398
LLPS-Urm-0409Ursus maritimus53.822e-135 399
LLPS-Orc-0575Oryctolagus cuniculus53.822e-129 384
LLPS-Otg-1768Otolemur garnettii53.824e-129 383
LLPS-Cap-2194Cavia porcellus53.821e-135 400
LLPS-Cas-1133Carlito syrichta53.823e-131 389
LLPS-Cae-1279Caenorhabditis elegans53.622e-137 404
LLPS-Mea-0852Mesocricetus auratus53.545e-136 400
LLPS-Mum-1654Mus musculus53.541e-135 400
LLPS-Mup-3097Mustela putorius furo53.546e-128 380
LLPS-Caf-1324Canis familiaris53.542e-128 381
LLPS-Ict-0100Ictidomys tridecemlineatus53.543e-135 399
LLPS-Nol-2261Nomascus leucogenys53.542e-130 386
LLPS-Chs-0899Chlorocebus sabaeus53.451e-129 383
LLPS-Crn-0475Cryptococcus neoformans53.411e-94 297
LLPS-Put-0960Puccinia triticina53.319e-89 277
LLPS-Cea-1265Cercocebus atys53.261e-129 384
LLPS-Maf-0185Macaca fascicularis53.261e-129 384
LLPS-Gog-2575Gorilla gorilla53.263e-130 386
LLPS-Bot-2572Bos taurus53.261e-130 387
LLPS-Paa-0443Papio anubis53.261e-129 384
LLPS-Mal-0375Mandrillus leucophaeus53.263e-129 383
LLPS-Ran-3939Rattus norvegicus53.261e-136 402
LLPS-Man-2691Macaca nemestrina53.261e-129 384
LLPS-Fec-2873Felis catus53.263e-131 389
LLPS-Pat-0485Pan troglodytes53.263e-130 386
LLPS-Pap-0468Pan paniscus53.263e-130 386
LLPS-Hos-0295Homo sapiens53.263e-130 386
LLPS-Rhb-4199Rhinopithecus bieti53.268e-130 385
LLPS-Mam-1545Macaca mulatta53.261e-129 384
LLPS-Poa-0768Pongo abelii53.263e-130 386
LLPS-Gas-0144Galdieria sulphuraria53.144e-128 380
LLPS-Sus-0666Sus scrofa53.122e-131 389
LLPS-Blg-0124Blumeria graminis53.124e-112 339
LLPS-Scj-0941Schizosaccharomyces japonicus53.123e-133 393
LLPS-Eqc-3248Equus caballus53.121e-128 382
LLPS-Pyt-0008Pyrenophora teres52.986e-105 320
LLPS-Tut-1192Tursiops truncatus52.979e-130 385
LLPS-Tar-1215Takifugu rubripes52.841e-138 409
LLPS-Asfu-0800Aspergillus fumigatus52.811e-105 323
LLPS-Mel-0715Melampsora laricipopulina52.591e-125 374
LLPS-Scc-0387Schizosaccharomyces cryophilus52.422e-132 392
LLPS-Ova-0866Ovis aries52.412e-126 375
LLPS-Fuo-0424Fusarium oxysporum52.382e-72 233
LLPS-Tum-0928Tuber melanosporum52.35e-117 356
LLPS-Tru-1605Triticum urartu52.31e-134 399
LLPS-Fuv-1008Fusarium verticillioides52.252e-126 377
LLPS-Abg-0288Absidia glauca52.16e-131 388
LLPS-Fus-0361Fusarium solani51.971e-125 375
LLPS-Aso-1382Aspergillus oryzae51.881e-105 322
LLPS-Nec-0556Neurospora crassa51.852e-124 371
LLPS-Anc-0569Anolis carolinensis51.859e-132 390
LLPS-Asc-0727Aspergillus clavatus51.711e-120 362
LLPS-Lem-0965Leptosphaeria maculans51.693e-123 370
LLPS-Nef-0948Neosartorya fischeri51.693e-121 363
LLPS-Pug-0221Puccinia graminis51.562e-127 379
LLPS-Asn-0735Aspergillus nidulans51.47e-127 378
LLPS-Cym-0423Cyanidioschyzon merolae51.122e-120 361
LLPS-Trv-0220Trichoderma virens51.128e-126 375
LLPS-Coo-0055Colletotrichum orbiculare51.01e-123 369
LLPS-Cog-0632Colletotrichum gloeosporioides51.06e-125 373
LLPS-Map-0262Magnaporthe poae50.992e-125 374
LLPS-Yal-0929Yarrowia lipolytica50.972e-125 374
LLPS-Scp-0251Schizosaccharomyces pombe50.856e-133 393
LLPS-Asf-0676Aspergillus flavus50.841e-119 359
LLPS-Beb-0696Beauveria bassiana50.715e-122 365
LLPS-Gag-0290Gaeumannomyces graminis50.76e-126 375
LLPS-Trr-0609Trichoderma reesei50.564e-124 371
LLPS-Kop-0554Komagataella pastoris50.433e-126 375
LLPS-Zyt-0203Zymoseptoria tritici50.438e-123 367
LLPS-Mao-0135Magnaporthe oryzae50.284e-123 368
LLPS-Asni-1077Aspergillus niger50.288e-123 367
LLPS-Pytr-0753Pyrenophora triticirepentis50.142e-119 359
LLPS-Cogr-0326Colletotrichum graminicola50.144e-120 360
LLPS-Ved-0019Verticillium dahliae50.09e-121 362
LLPS-Dos-1012Dothistroma septosporum49.711e-120 362
LLPS-Miv-0171Microbotryum violaceum49.586e-122 365
LLPS-Ast-0208Aspergillus terreus49.311e-120 362
LLPS-Usm-0027Ustilago maydis48.92e-121 363
LLPS-Spr-0902Sporisorium reilianum48.771e-119 359
LLPS-Chc-0557Chondrus crispus47.895e-105 322
LLPS-Phn-0829Phaeosphaeria nodorum47.627e-110 333
LLPS-Sac-1364Saccharomyces cerevisiae47.463e-115 348
LLPS-Asg-1164Ashbya gossypii46.632e-111 338
LLPS-Scs-0771Sclerotinia sclerotiorum46.29e-102 313
LLPS-Ten-1024Tetraodon nigroviridis42.985e-90 282
LLPS-Leo-1061Lepisosteus oculatus35.994e-50 178