• Disorder▼
  • Domain▼
  • PTM▼
  • Variation▼
  • Mutation▼
  • Interaction▼
  • Disease▼
  • Drug▼
  • Physicochemical▼
  • Function▼
  • Proteomics▼
  • Structure▼
  • Localization▼
  • Expression▼
  • Element▼
  • Methylation▼

LLPS-Tag-2635
FEN1

Integrated Annotations

▼ OVERVIEW

Status: Unreviewed
Protein Name: Flap endonuclease 1
Gene Name: FEN1
Ensembl Gene: ENSTGUG00000006627.1
Ensembl Protein: ENSTGUP00000006807.1
Organism: Taeniopygia guttata
Taxa ID: 59729
LLPS Type: LLPS regulator


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
Nucleolus, P-bodyPredicted from orthologs(View)

▼ FUNCTION

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

▼ CROSS REFERENCE

DatabaseNucleotide IDProtein ID
EnsemblENSTGUT00000006875.1ENSTGUP00000006807.1
UniProtH0Z8A4, H0Z8A4_TAEGU
GeneBankABQF01001603

▼ SEQUENCE

Protein Sequence (FASTA)
1     MGIHGLAKLI  ADVAPGAIRE  NDIKAYFGRK  VAIDASMSIY  QFLIAVRQGA  DVLQNEDGET  60
61    TSHLMGMFYR  TIRMVENGIK  PVYVFDGKPP  QLKSGELAKR  TERRAEAEKH  LQEAQEAGEE  120
121   ENIEKYSKRL  VKVTPQHTQE  CKKLLTLMGI  PYVEAPGEAE  ASCAALVKAG  KVYAAATEDM  180
181   DCLTFGSPVL  MRHLTASETK  KLPIQEFHLN  RILQDLQLTW  EQFVDLCILL  GCDYCASIRG  240
241   IGPKRAVELI  REHKSIERIV  QQLDTKKYPL  PENWLHREAQ  KLFLEPDVID  PDAVELKWSE  300
301   PDEEQLVQFM  CGEKQFNEER  IRNGVKRLSK  SRQGSTQGRL  DDFFKVTGSI  TSAKRKEPEP  360
361   KGGAKKKAKT  NS  372
Nucleotide CDS Sequence (FASTA)
1     ATGGGAATCC  ATGGCTTGGC  CAAGCTTATC  GCCGACGTGG  CTCCTGGGGC  CATCCGGGAG  60
61    AATGACATCA  AGGCCTACTT  TGGCCGGAAA  GTGGCCATCG  ACGCCTCCAT  GAGCATCTAC  120
121   CAGTTCCTGA  TTGCCGTGCG  GCAGGGAGCC  GATGTGCTGC  AGAACGAGGA  TGGGGAGACC  180
181   ACCAGCCACC  TGATGGGAAT  GTTCTACCGC  ACCATCCGCA  TGGTGGAGAA  TGGCATCAAG  240
241   CCGGTGTACG  TGTTCGACGG  GAAGCCCCCG  CAGCTGAAAT  CCGGGGAGCT  GGCCAAGCGC  300
301   ACGGAGCGCC  GGGCCGAGGC  AGAGAAACAC  CTGCAGGAAG  CACAGGAGGC  TGGGGAGGAG  360
361   GAGAACATCG  AGAAGTACAG  CAAGAGGCTG  GTCAAGGTGA  CCCCGCAGCA  CACGCAGGAG  420
421   TGCAAGAAGC  TGCTGACGCT  GATGGGAATT  CCCTATGTGG  AGGCACCCGG  AGAGGCAGAG  480
481   GCCAGCTGTG  CCGCCTTGGT  GAAGGCTGGG  AAGGTGTACG  CCGCCGCCAC  GGAGGACATG  540
541   GATTGCCTCA  CCTTCGGTAG  CCCCGTGCTG  ATGCGGCACC  TCACGGCCAG  TGAGACCAAG  600
601   AAGCTGCCCA  TCCAGGAGTT  CCACCTGAAC  CGGATTCTGC  AGGATCTGCA  GCTGACCTGG  660
661   GAGCAGTTTG  TGGACCTGTG  CATCCTGCTG  GGCTGCGACT  ACTGCGCCAG  CATCCGCGGC  720
721   ATCGGGCCGA  AGCGCGCCGT  GGAGCTCATC  CGGGAGCACA  AATCCATCGA  GAGGATCGTG  780
781   CAGCAGCTCG  ACACCAAGAA  GTACCCCCTG  CCTGAGAACT  GGCTGCACAG  GGAGGCCCAG  840
841   AAGCTCTTCC  TGGAGCCCGA  CGTGATCGAT  CCCGATGCCG  TGGAGCTGAA  GTGGAGCGAG  900
901   CCGGACGAGG  AGCAGCTGGT  GCAGTTTATG  TGTGGGGAGA  AGCAGTTCAA  TGAGGAGCGC  960
961   ATCCGCAACG  GTGTCAAGAG  GCTGAGCAAG  AGCCGCCAGG  GAAGCACCCA  GGGCCGGCTC  1020
1021  GACGACTTCT  TCAAGGTCAC  CGGATCCATC  ACCTCGGCCA  AGCGCAAGGA  GCCGGAGCCC  1080
1081  AAGGGAGGGG  CCAAGAAGAA  AGCCAAGACC  AACTCT  1116

▼ KEYWORD

ID
Family
Coiled coil
Complete proteome
DNA damage
DNA repair
DNA replication
Endonuclease
Exonuclease
Hydrolase
Magnesium
Metal-binding
Mitochondrion
Nuclease
Nucleus
Phosphoprotein
Reference proteome

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Mitochondrion
Cellular Component
Nuclear chromosome, telomeric region
Cellular Component
Nucleolus
Cellular Component
Nucleoplasm
Cellular Component
Protein-containing complex
Molecular Function
5'-3' exonuclease activity
Molecular Function
5'-flap endonuclease activity
Molecular Function
DNA binding
Molecular Function
Magnesium ion binding
Molecular Function
RNA-DNA hybrid ribonuclease activity
Biological Process
Base-excision repair
Biological Process
DNA replication
Biological Process
DNA replication, removal of RNA primer
Biological Process
Positive regulation of sister chromatid cohesion

▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Fia-1618Ficedula albicollis98.390.0 709
LLPS-Anp-0270Anas platyrhynchos92.960.0 676
LLPS-Pes-0376Pelodiscus sinensis92.110.0 669
LLPS-Meg-1791Meleagris gallopavo91.940.0 672
LLPS-Gaga-0195Gallus gallus91.940.0 674
LLPS-Anc-0569Anolis carolinensis85.590.0 620
LLPS-Sah-0179Sarcophilus harrisii85.350.0 615
LLPS-Eqc-3248Equus caballus85.350.0 608
LLPS-Mum-1654Mus musculus85.070.0 624
LLPS-Cas-1133Carlito syrichta85.070.0 621
LLPS-Caf-1324Canis familiaris85.070.0 610
LLPS-Mea-0852Mesocricetus auratus84.790.0 622
LLPS-Aim-0403Ailuropoda melanoleuca84.790.0 623
LLPS-Dio-2286Dipodomys ordii84.790.0 617
LLPS-Ict-0100Ictidomys tridecemlineatus84.790.0 622
LLPS-Cap-2194Cavia porcellus84.790.0 625
LLPS-Sus-0666Sus scrofa84.510.0 621
LLPS-Mup-3097Mustela putorius furo84.510.0 605
LLPS-Bot-2572Bos taurus84.510.0 616
LLPS-Otg-1768Otolemur garnettii84.510.0 607
LLPS-Urm-0409Ursus maritimus84.510.0 620
LLPS-Nol-2261Nomascus leucogenys84.510.0 610
LLPS-Chs-0899Chlorocebus sabaeus84.350.0 592
LLPS-Gog-2575Gorilla gorilla84.230.0 611
LLPS-Maf-0185Macaca fascicularis84.230.0 610
LLPS-Cea-1265Cercocebus atys84.230.0 610
LLPS-Aon-3219Aotus nancymaae84.230.0 611
LLPS-Paa-0443Papio anubis84.230.0 610
LLPS-Rhb-4199Rhinopithecus bieti84.230.0 610
LLPS-Fec-2873Felis catus84.230.0 618
LLPS-Hos-0295Homo sapiens84.230.0 611
LLPS-Pap-0468Pan paniscus84.230.0 611
LLPS-Pat-0485Pan troglodytes84.230.0 611
LLPS-Man-2691Macaca nemestrina84.230.0 610
LLPS-Poa-0768Pongo abelii84.230.0 611
LLPS-Mam-1545Macaca mulatta84.230.0 610
LLPS-Mal-0375Mandrillus leucophaeus83.940.0 607
LLPS-Orc-0575Oryctolagus cuniculus83.940.0 602
LLPS-Fud-0817Fukomys damarensis83.660.0 605
LLPS-Ran-3939Rattus norvegicus83.660.0 617
LLPS-Myl-2294Myotis lucifugus83.660.0 617
LLPS-Tut-1192Tursiops truncatus83.380.0 608
LLPS-Mod-2146Monodelphis domestica83.10.0 611
LLPS-Lac-1368Latimeria chalumnae81.441e-171 489
LLPS-Ova-0866Ovis aries81.410.0 593
LLPS-Xet-0658Xenopus tropicalis80.00.0 597
LLPS-Xim-1841Xiphophorus maculatus75.770.0 566
LLPS-Scf-2853Scleropages formosus75.770.0 568
LLPS-Dar-1793Danio rerio75.770.0 568
LLPS-Icp-0514Ictalurus punctatus75.490.0 562
LLPS-Orl-1540Oryzias latipes75.490.0 559
LLPS-Orn-3509Oreochromis niloticus75.490.0 563
LLPS-Asm-0753Astyanax mexicanus74.930.0 560
LLPS-Pof-0073Poecilia formosa74.930.0 561
LLPS-Gaa-0922Gasterosteus aculeatus74.650.0 558
LLPS-Scm-0534Scophthalmus maximus74.370.0 554
LLPS-Tar-1215Takifugu rubripes69.430.0 547
LLPS-Cii-0087Ciona intestinalis64.238e-170 486
LLPS-Cis-0672Ciona savignyi64.233e-171 490
LLPS-Drm-0311Drosophila melanogaster64.026e-167 479
LLPS-Cae-1279Caenorhabditis elegans62.42e-161 465
LLPS-Fuo-0424Fusarium oxysporum60.53e-89 276
LLPS-Scj-0941Schizosaccharomyces japonicus60.297e-149 433
LLPS-Scc-0387Schizosaccharomyces cryophilus60.122e-147 429
LLPS-Crn-0475Cryptococcus neoformans59.557e-105 323
LLPS-Fus-0361Fusarium solani59.436e-140 411
LLPS-Nef-0948Neosartorya fischeri59.432e-139 410
LLPS-Asfu-0800Aspergillus fumigatus58.924e-121 363
LLPS-Glm-1176Glycine max58.742e-145 426
LLPS-Fuv-1008Fusarium verticillioides58.694e-139 409
LLPS-Abg-0288Absidia glauca58.695e-146 426
LLPS-Asg-1164Ashbya gossypii58.596e-142 415
LLPS-Asf-0676Aspergillus flavus58.41e-135 400
LLPS-Beb-0696Beauveria bassiana58.41e-141 415
LLPS-Mel-0715Melampsora laricipopulina58.213e-135 398
LLPS-Brn-0901Brassica napus58.11e-144 424
LLPS-Brr-1381Brassica rapa58.11e-145 424
LLPS-Bro-2245Brassica oleracea58.11e-145 425
LLPS-Tum-0928Tuber melanosporum58.062e-134 400
LLPS-Scp-0251Schizosaccharomyces pombe58.07e-146 426
LLPS-Coo-0055Colletotrichum orbiculare57.837e-139 408
LLPS-Cog-0632Colletotrichum gloeosporioides57.832e-139 409
LLPS-Ved-0019Verticillium dahliae57.831e-137 405
LLPS-Arl-0879Arabidopsis lyrata57.821e-143 422
LLPS-Art-0221Arabidopsis thaliana57.825e-143 421
LLPS-Chr-0596Chlamydomonas reinhardtii57.87e-141 413
LLPS-Sac-1364Saccharomyces cerevisiae57.793e-142 416
LLPS-Aso-1382Aspergillus oryzae57.783e-119 357
LLPS-Dos-1012Dothistroma septosporum57.643e-139 409
LLPS-Cogr-0326Colletotrichum graminicola57.551e-136 402
LLPS-Asc-0727Aspergillus clavatus57.512e-136 402
LLPS-Lem-0965Leptosphaeria maculans57.433e-136 402
LLPS-Trr-0609Trichoderma reesei57.431e-137 405
LLPS-Trv-0220Trichoderma virens57.433e-138 406
LLPS-Map-0262Magnaporthe poae57.312e-136 402
LLPS-Nia-0211Nicotiana attenuata57.32e-146 427
LLPS-Nec-0556Neurospora crassa57.262e-135 399
LLPS-Orbr-0792Oryza brachyantha57.231e-139 409
LLPS-Asni-1077Aspergillus niger57.148e-137 403
LLPS-Pyt-0008Pyrenophora teres57.092e-113 341
LLPS-Mae-0099Manihot esculenta56.981e-142 417
LLPS-Ori-0397Oryza indica56.943e-138 406
LLPS-Asn-0735Aspergillus nidulans56.822e-139 409
LLPS-Viv-0328Vitis vinifera56.782e-143 419
LLPS-Mao-0135Magnaporthe oryzae56.734e-133 394
LLPS-Gag-0290Gaeumannomyces graminis56.731e-134 397
LLPS-Met-0011Medicago truncatula56.71e-142 418
LLPS-Hea-1035Helianthus annuus56.662e-141 414
LLPS-Sei-1775Setaria italica56.665e-137 407
LLPS-Ors-1482Oryza sativa56.651e-137 404
LLPS-Org-0494Oryza glaberrima56.651e-137 404
LLPS-Sem-1082Selaginella moellendorffii56.523e-137 403
LLPS-Pug-0221Puccinia graminis56.482e-132 391
LLPS-Zyt-0203Zymoseptoria tritici56.451e-138 407
LLPS-Phv-1094Phaseolus vulgaris56.411e-140 416
LLPS-Ast-0208Aspergillus terreus56.32e-134 397
LLPS-Pytr-0753Pyrenophora triticirepentis56.291e-132 392
LLPS-Cus-0532Cucumis sativus56.152e-140 413
LLPS-Via-0889Vigna angularis56.134e-142 417
LLPS-Prp-0732Prunus persica56.131e-140 416
LLPS-Blg-0124Blumeria graminis56.054e-120 360
LLPS-Mua-0298Musa acuminata56.022e-138 406
LLPS-Kop-0554Komagataella pastoris56.04e-135 398
LLPS-Yal-0929Yarrowia lipolytica55.951e-139 410
LLPS-Gor-0173Gossypium raimondii55.936e-141 413
LLPS-Pot-0330Populus trichocarpa55.915e-141 414
LLPS-Thc-1819Theobroma cacao55.525e-139 409
LLPS-Sob-0247Sorghum bicolor55.522e-138 406
LLPS-Dac-1311Daucus carota55.438e-139 408
LLPS-Usm-0027Ustilago maydis55.436e-129 382
LLPS-Ten-1024Tetraodon nigroviridis55.431e-136 402
LLPS-Osl-0082Ostreococcus lucimarinus55.372e-129 384
LLPS-Miv-0171Microbotryum violaceum55.286e-131 387
LLPS-Coc-1602Corchorus capsularis55.241e-138 408
LLPS-Brd-0390Brachypodium distachyon55.112e-136 401
LLPS-Spr-0902Sporisorium reilianum54.877e-129 382
LLPS-Tra-1696Triticum aestivum54.832e-135 399
LLPS-Put-0960Puccinia triticina54.695e-91 283
LLPS-Phn-0829Phaeosphaeria nodorum54.423e-122 365
LLPS-Php-0223Physcomitrella patens54.348e-138 405
LLPS-Hov-1098Hordeum vulgare54.339e-128 379
LLPS-Amt-0816Amborella trichopoda54.321e-135 399
LLPS-Zem-0587Zea mays53.262e-134 397
LLPS-Orp-1394Oryza punctata52.412e-122 365
LLPS-Orgl-1200Oryza glumaepatula52.356e-116 350
LLPS-Gas-0144Galdieria sulphuraria51.851e-122 366
LLPS-Lep-0111Leersia perrieri51.843e-123 367
LLPS-Cym-0423Cyanidioschyzon merolae51.552e-112 340
LLPS-Chc-0557Chondrus crispus51.142e-102 315
LLPS-Sol-0104Solanum lycopersicum50.533e-127 380
LLPS-Vir-1643Vigna radiata50.142e-123 370
LLPS-Orm-1176Oryza meridionalis49.532e-93 299
LLPS-Scs-0771Sclerotinia sclerotiorum48.621e-106 325
LLPS-Orb-1513Oryza barthii45.483e-84 267
LLPS-Orr-1609Oryza rufipogon45.178e-80 263
LLPS-Tru-1605Triticum urartu45.112e-91 288
LLPS-Sot-1640Solanum tuberosum44.634e-55 186
LLPS-Orni-0004Oryza nivara43.994e-80 265
LLPS-Leo-1061Lepisosteus oculatus37.741e-60 205