• Disorder▼
  • Domain▼
  • PTM▼
  • Variation▼
  • Mutation▼
  • Interaction▼
  • Disease▼
  • Drug▼
  • Physicochemical▼
  • Function▼
  • Proteomics▼
  • Structure▼
  • Localization▼
  • Expression▼
  • Element▼
  • Methylation▼

LLPS-Pat-0485
FEN1

Integrated Annotations

▼ OVERVIEW

Status: Unreviewed
Protein Name: Flap endonuclease 1
Gene Name: FEN1, CK820_G0030918
Ensembl Gene: ENSPTRG00000003751.3
Ensembl Protein: ENSPTRP00000006454.2
Organism: Pan troglodytes
Taxa ID: 9598
LLPS Type: LLPS regulator


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
Nucleolus, P-bodyPredicted from orthologs(View)

▼ FUNCTION

Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.

▼ SEQUENCE

Protein Sequence (FASTA)
1     MGIQGLAKLI  ADVAPSAIRE  NDIKSYFGRK  VAIDASMSIY  QFLIAVRQGG  DVLQNEEGET  60
61    TSHLMGMFYR  TIRMMENGIK  PVYVFDGKPP  QLKSGELAKR  SERRAEAEKQ  LQQAQAAGAE  120
121   QEVEKFTKRL  VKVTKQHNDE  CKHLLSLMGI  PYLDAPSEAE  ASCAALVKAG  KVYAAATEDM  180
181   DCLTFGSPVL  MRHLTASEAK  KLPIQEFHLS  RILQELGLNQ  EQFVDLCILL  GSDYCESIRG  240
241   IGPKRAVDLI  QKHKSIEEIV  RRLDPNKYPV  PENWLHKEAH  QLFLEPEVLD  PESVELKWSE  300
301   PNEEELIKFM  CGEKQFSEER  IRSGVKRLSK  SRQGSTQGRL  DDFFKVTGSL  SSAKRKEPEP  360
361   KGSTKKKAKT  GAAGKFKRGK  380
Nucleotide CDS Sequence (FASTA)
1     ATGGGAATTC  AAGGCCTGGC  CAAACTAATT  GCTGATGTGG  CCCCCAGTGC  CATCCGGGAG  60
61    AATGACATCA  AGAGCTACTT  TGGCCGTAAG  GTGGCCATTG  ATGCCTCTAT  GAGCATTTAT  120
121   CAGTTCCTGA  TTGCTGTTCG  CCAGGGTGGG  GATGTGCTGC  AGAATGAGGA  GGGTGAGACC  180
181   ACCAGCCACC  TGATGGGCAT  GTTCTACCGC  ACCATTCGCA  TGATGGAGAA  CGGCATCAAG  240
241   CCCGTGTATG  TCTTTGATGG  CAAGCCGCCA  CAGCTCAAGT  CAGGCGAGCT  GGCCAAACGC  300
301   AGTGAGCGGC  GGGCTGAGGC  AGAGAAGCAG  CTGCAGCAGG  CTCAGGCTGC  TGGGGCCGAG  360
361   CAGGAGGTGG  AAAAATTCAC  TAAGCGGCTG  GTGAAGGTCA  CTAAGCAGCA  CAATGATGAG  420
421   TGCAAACATC  TGCTGAGCCT  CATGGGCATC  CCTTATCTTG  ATGCACCCAG  TGAGGCAGAG  480
481   GCCAGCTGTG  CTGCCCTGGT  GAAGGCTGGC  AAAGTCTATG  CTGCGGCTAC  TGAGGACATG  540
541   GACTGCCTCA  CCTTCGGCAG  CCCTGTGCTA  ATGCGACACC  TGACTGCCAG  TGAAGCCAAA  600
601   AAGCTGCCAA  TCCAGGAATT  CCACCTGAGC  CGGATTCTGC  AGGAGCTGGG  CCTGAACCAG  660
661   GAACAGTTTG  TGGATCTGTG  CATCCTGCTA  GGCAGTGACT  ACTGTGAGAG  TATCCGGGGT  720
721   ATTGGGCCCA  AGCGGGCTGT  GGACCTCATC  CAGAAGCACA  AGAGCATCGA  GGAGATCGTG  780
781   CGGCGACTTG  ACCCCAACAA  GTACCCTGTG  CCAGAAAATT  GGCTCCACAA  GGAGGCTCAC  840
841   CAGCTCTTCT  TGGAACCTGA  GGTGCTGGAC  CCAGAGTCTG  TGGAGCTGAA  GTGGAGCGAG  900
901   CCAAATGAAG  AAGAGCTGAT  CAAGTTCATG  TGTGGTGAAA  AGCAGTTCTC  TGAGGAGCGA  960
961   ATCCGCAGTG  GGGTCAAGAG  GCTGAGTAAG  AGCCGCCAAG  GCAGCACCCA  GGGCCGCCTG  1020
1021  GATGATTTCT  TCAAGGTGAC  CGGCTCACTC  TCTTCAGCTA  AGCGCAAGGA  GCCAGAACCC  1080
1081  AAGGGATCCA  CTAAGAAGAA  GGCAAAGACT  GGGGCAGCAG  GGAAGTTTAA  AAGGGGAAAA  1140
1141  TAA  1143

▼ KEYWORD

ID
Family
Coiled coil
Complete proteome
DNA damage
DNA repair
DNA replication
Endonuclease
Exonuclease
Hydrolase
Magnesium
Metal-binding
Methylation
Mitochondrion
Nuclease
Nucleus
Phosphoprotein
Reference proteome

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Mitochondrion
Cellular Component
Nuclear chromosome, telomeric region
Cellular Component
Nucleolus
Cellular Component
Nucleoplasm
Cellular Component
Nucleus
Cellular Component
Protein-containing complex
Molecular Function
5'-3' exonuclease activity
Molecular Function
5'-flap endonuclease activity
Molecular Function
DNA binding
Molecular Function
Flap endonuclease activity
Molecular Function
Magnesium ion binding
Molecular Function
Manganese ion binding
Molecular Function
RNA-DNA hybrid ribonuclease activity
Biological Process
Base-excision repair
Biological Process
DNA replication
Biological Process
DNA replication, removal of RNA primer
Biological Process
Positive regulation of sister chromatid cohesion

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Gog-2575Gorilla gorilla100.00.0 696
LLPS-Poa-0768Pongo abelii100.00.0 696
LLPS-Hos-0295Homo sapiens100.00.0 696
LLPS-Pap-0468Pan paniscus100.00.0 696
LLPS-Nol-2261Nomascus leucogenys99.440.0 694
LLPS-Rhb-4199Rhinopithecus bieti99.440.0 694
LLPS-Paa-0443Papio anubis99.170.0 692
LLPS-Maf-0185Macaca fascicularis99.170.0 692
LLPS-Cea-1265Cercocebus atys99.170.0 692
LLPS-Mam-1545Macaca mulatta99.170.0 692
LLPS-Man-2691Macaca nemestrina99.170.0 692
LLPS-Chs-0899Chlorocebus sabaeus99.130.0 664
LLPS-Mal-0375Mandrillus leucophaeus98.890.0 691
LLPS-Bot-2572Bos taurus98.890.0 692
LLPS-Aon-3219Aotus nancymaae98.890.0 691
LLPS-Cas-1133Carlito syrichta98.610.0 689
LLPS-Ict-0100Ictidomys tridecemlineatus98.610.0 693
LLPS-Caf-1324Canis familiaris98.610.0 690
LLPS-Eqc-3248Equus caballus98.330.0 686
LLPS-Otg-1768Otolemur garnettii98.330.0 692
LLPS-Mea-0852Mesocricetus auratus98.050.0 691
LLPS-Aim-0403Ailuropoda melanoleuca97.780.0 689
LLPS-Tut-1192Tursiops truncatus97.780.0 687
LLPS-Orc-0575Oryctolagus cuniculus97.780.0 683
LLPS-Sus-0666Sus scrofa97.50.0 688
LLPS-Fec-2873Felis catus97.50.0 685
LLPS-Urm-0409Ursus maritimus97.50.0 687
LLPS-Mum-1654Mus musculus97.490.0 686
LLPS-Myl-2294Myotis lucifugus97.220.0 684
LLPS-Dio-2286Dipodomys ordii97.220.0 683
LLPS-Ran-3939Rattus norvegicus97.210.0 685
LLPS-Mup-3097Mustela putorius furo96.670.0 675
LLPS-Fud-0817Fukomys damarensis95.560.0 672
LLPS-Cap-2194Cavia porcellus95.00.0 668
LLPS-Ova-0866Ovis aries91.670.0 669
LLPS-Sah-0179Sarcophilus harrisii90.280.0 638
LLPS-Mod-2146Monodelphis domestica86.630.0 624
LLPS-Pes-0376Pelodiscus sinensis86.390.0 623
LLPS-Gaga-0195Gallus gallus85.240.0 624
LLPS-Meg-1791Meleagris gallopavo84.960.0 620
LLPS-Tag-2635Taeniopygia guttata84.440.0 612
LLPS-Fia-1618Ficedula albicollis84.440.0 611
LLPS-Anp-0270Anas platyrhynchos84.170.0 615
LLPS-Anc-0569Anolis carolinensis82.450.0 595
LLPS-Xet-0658Xenopus tropicalis80.50.0 593
LLPS-Lac-1368Latimeria chalumnae78.316e-165 472
LLPS-Dar-1793Danio rerio77.440.0 560
LLPS-Orl-1540Oryzias latipes76.880.0 546
LLPS-Orn-3509Oreochromis niloticus76.880.0 557
LLPS-Scf-2853Scleropages formosus76.040.0 555
LLPS-Icp-0514Ictalurus punctatus75.490.0 553
LLPS-Asm-0753Astyanax mexicanus75.490.0 549
LLPS-Xim-1841Xiphophorus maculatus74.930.0 550
LLPS-Gaa-0922Gasterosteus aculeatus74.930.0 542
LLPS-Pof-0073Poecilia formosa74.380.0 545
LLPS-Scm-0534Scophthalmus maximus74.090.0 540
LLPS-Tar-1215Takifugu rubripes69.740.0 533
LLPS-Cis-0672Ciona savignyi64.625e-168 482
LLPS-Fuo-0424Fusarium oxysporum64.599e-87 270
LLPS-Drm-0311Drosophila melanogaster63.915e-163 469
LLPS-Cii-0087Ciona intestinalis63.511e-165 476
LLPS-Cae-1279Caenorhabditis elegans63.231e-156 453
LLPS-Sac-1364Saccharomyces cerevisiae60.063e-142 417
LLPS-Scj-0941Schizosaccharomyces japonicus59.141e-148 432
LLPS-Asg-1164Ashbya gossypii59.143e-137 404
LLPS-Fus-0361Fusarium solani58.126e-135 399
LLPS-Crn-0475Cryptococcus neoformans57.959e-100 310
LLPS-Mae-0099Manihot esculenta57.52e-138 407
LLPS-Dos-1012Dothistroma septosporum57.353e-136 402
LLPS-Abg-0288Absidia glauca57.341e-140 412
LLPS-Ten-1024Tetraodon nigroviridis57.021e-137 404
LLPS-Scc-0387Schizosaccharomyces cryophilus56.953e-144 421
LLPS-Glm-1176Glycine max56.945e-139 409
LLPS-Beb-0696Beauveria bassiana56.72e-135 400
LLPS-Kop-0554Komagataella pastoris56.573e-133 394
LLPS-Fuv-1008Fusarium verticillioides56.578e-133 393
LLPS-Mel-0715Melampsora laricipopulina56.487e-133 393
LLPS-Scp-0251Schizosaccharomyces pombe56.41e-144 423
LLPS-Met-0011Medicago truncatula56.392e-137 405
LLPS-Bro-2245Brassica oleracea56.24e-138 406
LLPS-Gag-0290Gaeumannomyces graminis56.166e-131 388
LLPS-Nia-0211Nicotiana attenuata56.151e-140 412
LLPS-Nec-0556Neurospora crassa56.139e-132 390
LLPS-Lem-0965Leptosphaeria maculans56.134e-133 395
LLPS-Ved-0019Verticillium dahliae56.132e-133 395
LLPS-Cus-0532Cucumis sativus56.118e-137 404
LLPS-Asf-0676Aspergillus flavus56.06e-130 386
LLPS-Trv-0220Trichoderma virens56.02e-131 390
LLPS-Trr-0609Trichoderma reesei56.05e-131 389
LLPS-Dac-1311Daucus carota55.993e-134 397
LLPS-Brn-0901Brassica napus55.991e-136 404
LLPS-Brr-1381Brassica rapa55.992e-137 404
LLPS-Cogr-0326Colletotrichum graminicola55.893e-133 394
LLPS-Zyt-0203Zymoseptoria tritici55.872e-135 400
LLPS-Map-0262Magnaporthe poae55.879e-132 390
LLPS-Mao-0135Magnaporthe oryzae55.877e-131 388
LLPS-Gor-0173Gossypium raimondii55.838e-138 405
LLPS-Hea-1035Helianthus annuus55.84e-137 403
LLPS-Viv-0328Vitis vinifera55.84e-138 406
LLPS-Asn-0735Aspergillus nidulans55.712e-135 400
LLPS-Asni-1077Aspergillus niger55.712e-131 389
LLPS-Prp-0732Prunus persica55.564e-135 402
LLPS-Yal-0929Yarrowia lipolytica55.535e-135 399
LLPS-Thc-1819Theobroma cacao55.521e-133 396
LLPS-Coc-1602Corchorus capsularis55.523e-134 397
LLPS-Tum-0928Tuber melanosporum55.438e-134 399
LLPS-Nef-0948Neosartorya fischeri55.434e-134 396
LLPS-Pot-0330Populus trichocarpa55.433e-137 405
LLPS-Sem-1082Selaginella moellendorffii55.367e-131 387
LLPS-Phv-1094Phaseolus vulgaris55.281e-134 401
LLPS-Blg-0124Blumeria graminis55.242e-114 345
LLPS-Arl-0879Arabidopsis lyrata55.156e-135 400
LLPS-Art-0221Arabidopsis thaliana55.152e-134 399
LLPS-Aso-1382Aspergillus oryzae55.11e-112 340
LLPS-Cog-0632Colletotrichum gloeosporioides55.075e-134 396
LLPS-Via-0889Vigna angularis55.04e-137 404
LLPS-Sob-0247Sorghum bicolor54.872e-132 392
LLPS-Coo-0055Colletotrichum orbiculare54.799e-134 395
LLPS-Sei-1775Setaria italica54.725e-130 390
LLPS-Ast-0208Aspergillus terreus54.621e-128 383
LLPS-Asfu-0800Aspergillus fumigatus54.525e-115 347
LLPS-Osl-0082Ostreococcus lucimarinus54.442e-124 372
LLPS-Orbr-0792Oryza brachyantha54.443e-131 389
LLPS-Miv-0171Microbotryum violaceum54.292e-127 379
LLPS-Pug-0221Puccinia graminis54.182e-128 382
LLPS-Brd-0390Brachypodium distachyon54.172e-130 387
LLPS-Asc-0727Aspergillus clavatus54.141e-129 385
LLPS-Hov-1098Hordeum vulgare53.941e-122 366
LLPS-Ori-0397Oryza indica53.892e-129 384
LLPS-Org-0494Oryza glaberrima53.892e-129 384
LLPS-Ors-1482Oryza sativa53.892e-129 384
LLPS-Pyt-0008Pyrenophora teres53.873e-105 321
LLPS-Pytr-0753Pyrenophora triticirepentis53.856e-126 375
LLPS-Chr-0596Chlamydomonas reinhardtii53.741e-134 397
LLPS-Mua-0298Musa acuminata53.639e-130 385
LLPS-Php-0223Physcomitrella patens53.52e-130 387
LLPS-Tra-1921Triticum aestivum53.332e-128 382
LLPS-Spr-0902Sporisorium reilianum53.333e-126 375
LLPS-Amt-0816Amborella trichopoda53.25e-128 380
LLPS-Usm-0027Ustilago maydis53.062e-126 376
LLPS-Zem-0587Zea mays52.271e-127 380
LLPS-Gas-0144Galdieria sulphuraria51.571e-116 351
LLPS-Put-0960Puccinia triticina51.568e-86 270
LLPS-Phn-0829Phaeosphaeria nodorum51.02e-114 345
LLPS-Orgl-1200Oryza glumaepatula50.442e-107 329
LLPS-Orp-1394Oryza punctata50.286e-114 343
LLPS-Sol-0104Solanum lycopersicum49.879e-121 363
LLPS-Lep-0111Leersia perrieri49.726e-115 346
LLPS-Vir-1643Vigna radiata48.936e-118 356
LLPS-Cym-0423Cyanidioschyzon merolae48.786e-109 331
LLPS-Chc-0557Chondrus crispus48.592e-96 300
LLPS-Orm-1176Oryza meridionalis48.545e-90 290
LLPS-Scs-0771Sclerotinia sclerotiorum47.244e-104 319
LLPS-Orb-1513Oryza barthii45.794e-81 259
LLPS-Tru-1605Triticum urartu45.683e-89 282
LLPS-Orr-1609Oryza rufipogon45.488e-77 256
LLPS-Orni-0004Oryza nivara44.512e-77 259
LLPS-Sot-1640Solanum tuberosum43.583e-52 179
LLPS-Leo-1061Lepisosteus oculatus39.312e-63 213