LLPS-Sac-0411
RRP8
▼ OVERVIEW
| Status: | Reviewed |
| Protein Name: | 25S rRNA (adenine(645)-N(1))-methyltransferase; Ribosomal RNA-processing protein 8 |
| Gene Name: | RRP8, YDR083W, D4461 |
| Ensembl Gene: | YDR083W |
| Ensembl Protein: | YDR083W_mRNA |
| Organism: | Saccharomyces cerevisiae |
| Taxa ID: | 559292 |
| LLPS Type: | LLPS regulator |
▼ Classification
Condensates:
| Condensate | Description | Tissue/Cell | PMIDs |
|---|---|---|---|
| Nucleolus | "...Out of the 142 yeast nucleolar proteins that have at least one human homologue, 124 are found in the updated nucleolar proteome." | Saccharomyces cerevisiae | 15635413 |
| P-body | Predicted from orthologs | N/A | (View) |
▼ FUNCTION
| S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(1) position of adenine 645 in 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis. Required for efficient pre-rRNA cleavage at site A2. Also involved in telomere length regulation and maintenance. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | YDR083W_mRNA | YDR083W_mRNA |
| UniProt | P38961, RRP8_YEAST, Q7LHG2 | |
| GeneBank | BK006938, X82086, Z46796, Z74379 | CAA86805.1, CAA98903.1, CAA57610.1, DAA11930.1 |
| RefSeq | NM_001180391.3 | NP_010368.5 |
| Entrez | 851656 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MALFNVEGWS IKTKTVAFDN KTNKSSKDKK KNNRKNGKLT REQKLKEETE AELKEQVEDI 60 61 PSEGSVAKDI PKKNQEKSDQ NETSKKRKHD EEAPLMQVKE NIEKPTKKQL TPLQQKMMAK 120 121 LTGSRFRWIN EQLYTISSDE ALKLIKEQPQ LFDEYHDGFR SQVQAWPENP VDVFVDQIRY 180 181 RCMKPVNAPG GLPGLKDSKE IVIADMGCGE AQLALEINNF FKNYNKKAKK YLKRRHKVHS 240 241 FDLKKANERI TVADIRNVPL PDESCTIVVF CLALMGTNFL DFIKEAYRIL APRGELWIAE 300 301 IKSRFSDGKG NEFVDALKLM GFFHKKTFDE NKMFTRFEFF KPPAEIIEER RQKLERRQKF 360 361 IEVETEKEEL EKKRRKIAEG KWLLKPCIYK RR 392 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGGCCTTAT TTAACGTAGA AGGTTGGTCT ATTAAGACAA AAACCGTCGC TTTTGATAAC 60 61 AAGACTAATA AGTCTTCAAA AGATAAAAAA AAAAATAATA GAAAAAATGG CAAACTTACA 120 121 AGAGAACAGA AGTTGAAAGA AGAGACAGAG GCTGAGTTGA AGGAACAAGT GGAAGACATT 180 181 CCTTCCGAAG GATCAGTAGC CAAAGACATC CCAAAAAAAA ACCAAGAGAA AAGTGATCAA 240 241 AATGAAACGA GCAAGAAACG CAAGCACGAT GAAGAAGCCC CTCTGATGCA AGTAAAAGAA 300 301 AATATTGAAA AACCTACAAA GAAGCAACTA ACCCCTTTAC AACAAAAGAT GATGGCTAAA 360 361 CTGACTGGTT CTAGATTTAG ATGGATCAAT GAACAACTGT ATACAATTAG CTCTGATGAA 420 421 GCTTTGAAAT TAATAAAAGA ACAACCACAA TTATTTGACG AATATCATGA TGGTTTTAGA 480 481 TCACAAGTGC AAGCATGGCC GGAAAATCCA GTTGATGTTT TTGTTGACCA AATTCGTTAT 540 541 AGATGCATGA AACCTGTGAA TGCTCCAGGT GGGTTACCAG GTCTTAAGGA TAGTAAAGAA 600 601 ATAGTTATTG CTGATATGGG GTGTGGTGAA GCTCAATTAG CATTAGAAAT CAACAATTTT 660 661 TTCAAAAATT ACAATAAGAA AGCGAAGAAA TATTTGAAAA GACGCCATAA AGTCCACAGT 720 721 TTTGATTTGA AGAAAGCTAA CGAAAGAATA ACTGTGGCAG ATATTAGAAA TGTGCCGCTA 780 781 CCAGATGAGT CCTGTACTAT AGTGGTCTTC TGCCTGGCTC TAATGGGTAC AAATTTCCTC 840 841 GATTTCATAA AAGAAGCTTA TAGGATTTTA GCGCCAAGGG GTGAATTATG GATCGCAGAA 900 901 ATTAAATCAA GGTTTAGTGA CGGCAAAGGT AATGAATTTG TAGACGCTTT GAAGCTGATG 960 961 GGATTTTTTC ACAAAAAGAC CTTCGACGAG AATAAGATGT TTACAAGATT CGAATTTTTC 1020 1021 AAGCCACCTG CGGAGATTAT TGAAGAGAGA AGGCAGAAAT TGGAAAGAAG ACAAAAGTTT 1080 1081 ATTGAAGTTG AAACTGAGAA GGAAGAATTA GAAAAGAAAA GACGGAAAAT TGCCGAAGGA 1140 1141 AAATGGCTCT TGAAGCCCTG TATTTATAAA AGAAGATAA 1179 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | 90S preribosome | |
Cellular Component | Chromosome, telomeric region | |
Cellular Component | Nucleolus | |
Molecular Function | RRNA (adenine) methyltransferase activity | |
Molecular Function | RRNA (adenine-N1-)-methyltransferase activity | |
Biological Process | Ribosomal large subunit biogenesis | |
Biological Process | RRNA methylation | |
Biological Process | RRNA processing |
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Asg-0808 | Ashbya gossypii | 65.82 | 4e-138 | 407 |
| LLPS-Kop-0331 | Komagataella pastoris | 65.8 | 9e-120 | 359 |
| LLPS-Yal-0333 | Yarrowia lipolytica | 60.14 | 7e-105 | 323 |
| LLPS-Fus-0705 | Fusarium solani | 49.75 | 5e-56 | 197 |
| LLPS-Fuv-0655 | Fusarium verticillioides | 48.58 | 5e-54 | 192 |
| LLPS-Fuo-0734 | Fusarium oxysporum | 48.11 | 1e-53 | 191 |
| LLPS-Tum-1298 | Tuber melanosporum | 46.62 | 2e-59 | 207 |
| LLPS-Scp-0198 | Schizosaccharomyces pombe | 46.55 | 2e-70 | 230 |
| LLPS-Scs-0467 | Sclerotinia sclerotiorum | 46.3 | 4e-49 | 179 |
| LLPS-Trr-0605 | Trichoderma reesei | 46.26 | 1e-53 | 191 |
| LLPS-Coo-0242 | Colletotrichum orbiculare | 45.54 | 2e-52 | 189 |
| LLPS-Cogr-0761 | Colletotrichum graminicola | 44.98 | 1e-50 | 183 |
| LLPS-Nec-0045 | Neurospora crassa | 44.91 | 5e-49 | 179 |
| LLPS-Mao-0655 | Magnaporthe oryzae | 44.34 | 4e-48 | 176 |
| LLPS-Gaga-0848 | Gallus gallus | 44.32 | 4e-12 | 68.2 |
| LLPS-Aso-0527 | Aspergillus oryzae | 43.46 | 7e-44 | 164 |
| LLPS-Asf-0841 | Aspergillus flavus | 43.46 | 7e-44 | 164 |
| LLPS-Ten-0439 | Tetraodon nigroviridis | 43.44 | 2e-24 | 101 |
| LLPS-Ast-0662 | Aspergillus terreus | 42.92 | 1e-41 | 159 |
| LLPS-Dos-0808 | Dothistroma septosporum | 42.72 | 4e-46 | 167 |
| LLPS-Scj-0127 | Schizosaccharomyces japonicus | 42.65 | 2e-66 | 220 |
| LLPS-Asn-0199 | Aspergillus nidulans | 42.63 | 4e-51 | 182 |
| LLPS-Trv-0233 | Trichoderma virens | 42.57 | 3e-54 | 192 |
| LLPS-Pytr-0209 | Pyrenophora triticirepentis | 42.44 | 3e-47 | 174 |
| LLPS-Pyt-1380 | Pyrenophora teres | 42.44 | 7e-47 | 174 |
| LLPS-Nef-0307 | Neosartorya fischeri | 42.4 | 3e-42 | 160 |
| LLPS-Lem-0063 | Leptosphaeria maculans | 42.37 | 3e-44 | 167 |
| LLPS-Asc-0607 | Aspergillus clavatus | 42.33 | 4e-41 | 157 |
| LLPS-Asni-0933 | Aspergillus niger | 42.33 | 6e-41 | 156 |
| LLPS-Chc-0111 | Chondrus crispus | 42.11 | 2e-41 | 152 |
| LLPS-Beb-1065 | Beauveria bassiana | 42.08 | 9e-52 | 186 |
| LLPS-Scm-0461 | Scophthalmus maximus | 41.99 | 9e-46 | 170 |
| LLPS-Lac-2347 | Latimeria chalumnae | 41.92 | 4e-48 | 176 |
| LLPS-Asfu-0171 | Aspergillus fumigatus | 41.67 | 8e-41 | 156 |
| LLPS-Zyt-0388 | Zymoseptoria tritici | 41.47 | 3e-42 | 160 |
| LLPS-Tar-0925 | Takifugu rubripes | 41.45 | 2e-46 | 172 |
| LLPS-Scc-0448 | Schizosaccharomyces cryophilus | 41.32 | 3e-62 | 209 |
| LLPS-Cis-0199 | Ciona savignyi | 41.25 | 1e-48 | 176 |
| LLPS-Ved-0418 | Verticillium dahliae | 41.18 | 2e-37 | 145 |
| LLPS-Anp-0249 | Anas platyrhynchos | 41.11 | 3e-14 | 72.4 |
| LLPS-Phn-0048 | Phaeosphaeria nodorum | 40.83 | 2e-44 | 167 |
| LLPS-Dar-0853 | Danio rerio | 40.69 | 1e-43 | 164 |
| LLPS-Scf-0277 | Scleropages formosus | 40.61 | 2e-44 | 167 |
| LLPS-Gag-0410 | Gaeumannomyces graminis | 40.52 | 3e-45 | 169 |
| LLPS-Sah-1728 | Sarcophilus harrisii | 40.48 | 5e-43 | 162 |
| LLPS-Osl-0230 | Ostreococcus lucimarinus | 40.43 | 8e-47 | 166 |
| LLPS-Nol-0592 | Nomascus leucogenys | 40.34 | 1e-42 | 160 |
| LLPS-Fud-0105 | Fukomys damarensis | 40.0 | 5e-41 | 155 |
| LLPS-Bot-0446 | Bos taurus | 39.91 | 3e-42 | 159 |
| LLPS-Otg-0658 | Otolemur garnettii | 39.74 | 4e-42 | 159 |
| LLPS-Mae-0694 | Manihot esculenta | 39.57 | 1e-46 | 167 |
| LLPS-Aim-0903 | Ailuropoda melanoleuca | 39.48 | 1e-40 | 155 |
| LLPS-Orl-0588 | Oryzias latipes | 39.3 | 1e-42 | 161 |
| LLPS-Ova-1131 | Ovis aries | 39.06 | 9e-42 | 159 |
| LLPS-Sus-2175 | Sus scrofa | 39.06 | 6e-42 | 159 |
| LLPS-Gaa-0321 | Gasterosteus aculeatus | 38.91 | 5e-44 | 162 |
| LLPS-Chr-0347 | Chlamydomonas reinhardtii | 38.74 | 2e-39 | 155 |
| LLPS-Fec-1446 | Felis catus | 38.56 | 8e-38 | 147 |
| LLPS-Xim-0467 | Xiphophorus maculatus | 38.43 | 3e-44 | 166 |
| LLPS-Leo-0262 | Lepisosteus oculatus | 38.4 | 4e-45 | 168 |
| LLPS-Coc-0689 | Corchorus capsularis | 38.26 | 3e-44 | 160 |
| LLPS-Cap-0629 | Cavia porcellus | 38.26 | 2e-43 | 163 |
| LLPS-Thc-1210 | Theobroma cacao | 38.26 | 4e-45 | 163 |
| LLPS-Cii-0072 | Ciona intestinalis | 38.21 | 6e-33 | 130 |
| LLPS-Prp-1205 | Prunus persica | 38.16 | 5e-43 | 157 |
| LLPS-Pof-2875 | Poecilia formosa | 37.99 | 5e-43 | 162 |
| LLPS-Pot-0046 | Populus trichocarpa | 37.83 | 9e-44 | 159 |
| LLPS-Icp-0370 | Ictalurus punctatus | 37.6 | 5e-41 | 157 |
| LLPS-Brr-1138 | Brassica rapa | 37.55 | 5e-39 | 147 |
| LLPS-Abg-0144 | Absidia glauca | 37.5 | 2e-56 | 199 |
| LLPS-Cae-0099 | Caenorhabditis elegans | 37.39 | 2e-36 | 141 |
| LLPS-Sot-0078 | Solanum tuberosum | 37.29 | 2e-42 | 155 |
| LLPS-Gas-0628 | Galdieria sulphuraria | 37.07 | 7e-32 | 126 |
| LLPS-Cym-0059 | Cyanidioschyzon merolae | 37.04 | 7e-34 | 133 |
| LLPS-Glm-1307 | Glycine max | 36.97 | 3e-45 | 162 |
| LLPS-Asm-0793 | Astyanax mexicanus | 36.71 | 9e-40 | 153 |
| LLPS-Dac-0985 | Daucus carota | 36.56 | 2e-41 | 151 |
| LLPS-Orc-1022 | Oryctolagus cuniculus | 36.52 | 1e-44 | 166 |
| LLPS-Tut-0885 | Tursiops truncatus | 36.52 | 2e-43 | 162 |
| LLPS-Mam-0486 | Macaca mulatta | 36.33 | 1e-45 | 169 |
| LLPS-Hos-2271 | Homo sapiens | 36.33 | 3e-43 | 162 |
| LLPS-Pap-3345 | Pan paniscus | 36.33 | 1e-43 | 163 |
| LLPS-Phv-1750 | Phaseolus vulgaris | 36.33 | 1e-45 | 164 |
| LLPS-Maf-2170 | Macaca fascicularis | 36.33 | 1e-45 | 169 |
| LLPS-Chs-0104 | Chlorocebus sabaeus | 36.33 | 1e-45 | 169 |
| LLPS-Via-1693 | Vigna angularis | 36.32 | 6e-43 | 155 |
| LLPS-Mup-0233 | Mustela putorius furo | 36.24 | 8e-45 | 166 |
| LLPS-Aon-2597 | Aotus nancymaae | 36.21 | 3e-44 | 164 |
| LLPS-Caj-1067 | Callithrix jacchus | 36.21 | 1e-43 | 163 |
| LLPS-Poa-0581 | Pongo abelii | 36.15 | 8e-44 | 163 |
| LLPS-Paa-0380 | Papio anubis | 36.15 | 2e-45 | 168 |
| LLPS-Mal-2764 | Mandrillus leucophaeus | 36.15 | 8e-46 | 169 |
| LLPS-Cea-3250 | Cercocebus atys | 36.15 | 9e-46 | 169 |
| LLPS-Gog-0594 | Gorilla gorilla | 36.15 | 1e-43 | 163 |
| LLPS-Php-0688 | Physcomitrella patens | 36.07 | 1e-39 | 150 |
| LLPS-Rhb-1068 | Rhinopithecus bieti | 36.0 | 3e-45 | 167 |
| LLPS-Nia-1744 | Nicotiana attenuata | 35.9 | 1e-41 | 153 |
| LLPS-Ict-0549 | Ictidomys tridecemlineatus | 35.88 | 7e-44 | 164 |
| LLPS-Loa-0748 | Loxodonta africana | 35.88 | 2e-45 | 168 |
| LLPS-Pat-1796 | Pan troglodytes | 35.81 | 9e-43 | 160 |
| LLPS-Orn-0120 | Oreochromis niloticus | 35.81 | 9e-21 | 97.1 |
| LLPS-Gor-1398 | Gossypium raimondii | 35.8 | 4e-42 | 155 |
| LLPS-Amt-1433 | Amborella trichopoda | 35.77 | 2e-44 | 161 |
| LLPS-Cas-1497 | Carlito syrichta | 35.69 | 9e-44 | 163 |
| LLPS-Man-0641 | Macaca nemestrina | 35.67 | 3e-45 | 168 |
| LLPS-Tag-1216 | Taeniopygia guttata | 35.53 | 6e-36 | 136 |
| LLPS-Blg-0367 | Blumeria graminis | 35.46 | 2e-45 | 169 |
| LLPS-Eqc-0261 | Equus caballus | 35.25 | 7e-42 | 159 |
| LLPS-Drm-0387 | Drosophila melanogaster | 35.02 | 2e-31 | 127 |
| LLPS-Met-1243 | Medicago truncatula | 34.85 | 4e-43 | 157 |
| LLPS-Cus-0300 | Cucumis sativus | 34.78 | 1e-38 | 145 |
| LLPS-Ran-0257 | Rattus norvegicus | 34.75 | 5e-41 | 156 |
| LLPS-Pug-0901 | Puccinia graminis | 34.71 | 2e-31 | 127 |
| LLPS-Caf-1182 | Canis familiaris | 34.68 | 3e-42 | 161 |
| LLPS-Viv-0322 | Vitis vinifera | 34.67 | 7e-45 | 162 |
| LLPS-Dio-1199 | Dipodomys ordii | 34.67 | 1e-42 | 160 |
| LLPS-Sem-0503 | Selaginella moellendorffii | 34.67 | 7e-37 | 139 |
| LLPS-Art-2345 | Arabidopsis thaliana | 34.59 | 2e-43 | 159 |
| LLPS-Myl-1292 | Myotis lucifugus | 34.34 | 4e-43 | 162 |
| LLPS-Urm-2474 | Ursus maritimus | 34.33 | 6e-42 | 159 |
| LLPS-Hea-0210 | Helianthus annuus | 34.28 | 8e-42 | 154 |
| LLPS-Orbr-1899 | Oryza brachyantha | 34.03 | 2e-36 | 140 |
| LLPS-Tra-1054 | Triticum aestivum | 34.02 | 4e-40 | 150 |
| LLPS-Mum-1735 | Mus musculus | 33.89 | 6e-41 | 156 |
| LLPS-Spr-0447 | Sporisorium reilianum | 33.86 | 8e-44 | 165 |
| LLPS-Mea-2335 | Mesocricetus auratus | 33.69 | 4e-38 | 148 |
| LLPS-Brd-1325 | Brachypodium distachyon | 33.63 | 1e-37 | 143 |
| LLPS-Orb-0594 | Oryza barthii | 33.19 | 3e-37 | 140 |
| LLPS-Bro-0673 | Brassica oleracea | 33.09 | 3e-42 | 155 |
| LLPS-Brn-1071 | Brassica napus | 33.09 | 3e-42 | 155 |
| LLPS-Hov-0085 | Hordeum vulgare | 33.06 | 2e-36 | 140 |
| LLPS-Usm-0129 | Ustilago maydis | 33.04 | 5e-41 | 157 |
| LLPS-Sei-0867 | Setaria italica | 33.04 | 3e-35 | 136 |
| LLPS-Arl-1362 | Arabidopsis lyrata | 32.99 | 2e-42 | 156 |
| LLPS-Orgl-0155 | Oryza glumaepatula | 32.92 | 8e-38 | 144 |
| LLPS-Lep-0887 | Leersia perrieri | 32.64 | 9e-38 | 143 |
| LLPS-Mua-0253 | Musa acuminata | 32.38 | 1e-38 | 144 |
| LLPS-Sob-0743 | Sorghum bicolor | 32.37 | 3e-36 | 139 |
| LLPS-Zem-1612 | Zea mays | 31.95 | 1e-35 | 137 |
| LLPS-Put-1012 | Puccinia triticina | 31.9 | 3e-15 | 79.0 |
| LLPS-Orr-1760 | Oryza rufipogon | 31.8 | 4e-38 | 144 |
| LLPS-Org-1242 | Oryza glaberrima | 31.8 | 5e-38 | 144 |
| LLPS-Ors-1232 | Oryza sativa | 31.8 | 4e-38 | 144 |
| LLPS-Ori-0833 | Oryza indica | 31.8 | 4e-38 | 144 |
| LLPS-Orni-0937 | Oryza nivara | 31.8 | 4e-38 | 144 |
| LLPS-Orp-0917 | Oryza punctata | 31.42 | 2e-35 | 137 |
| LLPS-Sol-0540 | Solanum lycopersicum | 31.32 | 1e-34 | 134 |
| LLPS-Mel-0306 | Melampsora laricipopulina | 31.2 | 4e-33 | 132 |
| LLPS-Miv-0643 | Microbotryum violaceum | 30.55 | 8e-36 | 140 |
| LLPS-Crn-0419 | Cryptococcus neoformans | 30.48 | 9e-27 | 114 |