LLPS-Ran-1813
Picalm

▼ OVERVIEW

Status: Unreviewed
Protein Name: Phosphatidylinositol-binding clathrin assembly protein; Clathrin assembly lymphoid myeloid leukemia protein; rCALM
Gene Name: Picalm, Calm
Ensembl Gene: ENSRNOG00000018322.8
Ensembl Protein: ENSRNOP00000025415.6
Organism: Rattus norvegicus
Taxa ID: 10116
LLPS Type: Others
PDB: 1HF8 (A) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
Nucleolus, Postsynaptic densityPredicted from orthologs(View)

▼ FUNCTION

Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MSGQSLTDRI  TAAQHSVTGS  AVSKTVCKAT  THEIMGPKKK  HLDYLIQCTN  EMNVNIPQLA  60
61    DSLFERTTNS  SWVVVFKSLI  TTHHLMVYGN  ERFIQYLASR  NTLFNLSNFL  DKSGLQGYDM  120
121   STFIRRYSRY  LNEKAVSYRQ  VAFDFTKVKR  GADGVMRTMN  TEKLLKTVPI  IQNQMDALLD  180
181   FNVNSNELTN  GVINAAFMLL  FKDAIRLFAA  YNEGIINLLE  KYFDMKKNQC  KEGLDIYKKF  240
241   LTRMTRISEF  LKVAEQVGID  RGDIPDLSQA  PSSLLDALEQ  HLASLEGKKI  KDSTAASRAT  300
301   TLSNAVSSLA  STGLSLTKVD  EREKQAALEE  EQARLKALKE  QRLKELAKKP  HTSLTTAASP  360
361   VSTSAGGIMT  APAIDIFSTP  SSSNSTSKLP  NDLLDLQQPT  FHPSVHAMSA  APQVASTWGD  420
421   PFSATLDAVD  DAIPSLNPFL  TKSSGDVHLP  ISSDVSTFTT  RTPTHEMFVG  FSPSPVTQPH  480
481   PSAGLNVDFE  SVFGNKSTNV  AVDSGGGLLK  PTVASQNQSL  PVAKLAPNKL  VSDDLDSSLA  540
541   NLVGNLGIGN  GTTKNDVSWS  QPGEKKLTGG  SNWQPKVAPT  TAWSAATMNG  MHFPQYAPPV  600
601   MAYPATTPTG  MIGYGIPPQM  GSVPVMTQPT  LIYSQPVMRP  PNPFGPVPGA  QIQFM  655
Nucleotide CDS Sequence (FASTA)
1     ATGTCTGGCC  AGAGCCTGAC  GGACCGAATC  ACCGCGGCCC  AGCACAGTGT  CACTGGCTCC  60
61    GCGGTATCTA  AGACAGTATG  CAAGGCCACG  ACCCACGAGA  TCATGGGCCC  CAAGAAAAAG  120
121   CACCTGGACT  ACTTAATTCA  GTGTACAAAT  GAGATGAATG  TGAATATCCC  ACAGTTGGCA  180
181   GACAGTTTGT  TTGAAAGAAC  TACTAATAGT  AGTTGGGTGG  TGGTCTTCAA  ATCACTCATT  240
241   ACAACTCATC  ATTTGATGGT  GTATGGAAAT  GAGCGTTTCA  TTCAGTATTT  GGCTTCAAGA  300
301   AACACATTGT  TTAACTTAAG  CAACTTTTTG  GATAAAAGTG  GATTGCAAGG  ATATGATATG  360
361   TCTACATTTA  TTAGACGATA  TAGTAGGTAC  CTAAATGAAA  AGGCAGTTTC  ATACAGACAA  420
421   GTTGCATTCG  ATTTCACAAA  AGTGAAGAGA  GGAGCTGATG  GAGTTATGAG  AACAATGAAC  480
481   ACAGAAAAAC  TGTTAAAAAC  TGTACCAATT  ATCCAAAATC  AAATGGATGC  ACTTCTTGAT  540
541   TTTAATGTTA  ATAGTAATGA  ACTTACAAAT  GGGGTAATAA  ATGCTGCCTT  CATGCTCCTG  600
601   TTCAAAGATG  CCATTAGACT  ATTTGCAGCA  TACAATGAAG  GAATTATTAA  TTTATTGGAA  660
661   AAATATTTTG  ATATGAAAAA  GAACCAGTGC  AAAGAAGGTC  TTGACATCTA  TAAGAAGTTT  720
721   TTGACTAGGA  TGACAAGAAT  CTCAGAGTTT  CTGAAAGTTG  CAGAGCAAGT  TGGAATTGAC  780
781   AGAGGAGATA  TTCCAGATCT  TTCACAGGCC  CCCAGCAGTC  TTCTTGATGC  TTTAGAACAA  840
841   CATTTAGCTT  CCTTGGAAGG  GAAGAAAATC  AAAGATTCCA  CAGCTGCAAG  CAGGGCTACA  900
901   ACACTTTCCA  ATGCAGTCTC  TTCTTTGGCA  AGCACTGGCC  TATCTCTGAC  CAAAGTGGAT  960
961   GAAAGGGAAA  AGCAGGCAGC  ATTAGAGGAA  GAACAGGCTC  GATTAAAAGC  ACTAAAGGAA  1020
1021  CAGCGTCTAA  AAGAACTTGC  AAAGAAACCG  CATACCTCTT  TAACAACTGC  AGCCTCTCCT  1080
1081  GTGTCCACCT  CAGCAGGGGG  AATAATGACT  GCACCAGCCA  TCGACATATT  TTCTACCCCT  1140
1141  AGTTCTTCTA  ACAGCACATC  CAAGCTGCCA  AATGACCTGC  TTGATTTGCA  GCAGCCAACC  1200
1201  TTTCATCCAT  CTGTCCATGC  TATGTCAGCT  GCTCCTCAGG  TAGCAAGTAC  ATGGGGAGGA  1260
1261  TTCAGTCCTT  CTCCGGTTAC  ACAGCCACAT  CCTTCAGCTG  GCCTTAATGT  TGACTTTGAA  1320
1321  TCTGTGTTTG  GAAATAAGTC  TACGAATGTT  GCTGTAGATT  CTGGTGGCTT  TGATGAACTA  1380
1381  GGTGGACTTC  TCAAACCAAC  AGTGGCCTCT  CAGAACCAGA  GTCTTCCTGT  TGCCAAACTT  1440
1441  GCGCCTAACA  AATTAGTGTC  TGATGACTTG  GATTCATCTT  TAGCCAACCT  TGTGGGCAAT  1500
1501  CTTGGCATTG  GAAATGGAAC  CACTAAGAAT  GATGTAAGTT  GGAGTCAACC  AGGTGAAAAG  1560
1561  AAGTTAACTG  GAGGATCTAA  CTGGCAACCA  AAGGTTGCAC  CAACAACTGC  CTGGAGTGCT  1620
1621  GCAACAATGA  ATGGCATGCA  TTTTCCACAA  TACGCACCCC  CTGTAATGGC  CTATCCTGCT  1680
1681  ACTACACCAA  CGGGCATGAT  AGGATATGGA  ATTCCTCCTC  AGATGGGAAG  TGTACCTGTA  1740
1741  ATGACACAGC  CAACCTTAAT  ATACAGCCAG  CCTGTCATGA  GACCGCCAAA  CCCCTTTGGC  1800
1801  CCTGTACCAG  GAGCACAGAT  ACAGTTTATG  TAA  1833

▼ KEYWORD

ID
Family
3D-structure
Acetylation
Alternative splicing
Cell membrane
Coated pit
Complete proteome
Cytoplasmic vesicle
Endocytosis
Golgi apparatus
Isopeptide bond
Membrane
Nucleus
Phosphoprotein
Reference proteome
Ubl conjugation

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Clathrin-coated pit
Cellular Component
Clathrin-coated vesicle
Cellular Component
Endosome
Cellular Component
Extrinsic component of presynaptic endocytic zone membrane
Cellular Component
Golgi apparatus
Cellular Component
Intrinsic component of membrane
Cellular Component
Neurofibrillary tangle
Cellular Component
Neuron projection
Cellular Component
Neuronal cell body
Cellular Component
Nucleus
Cellular Component
Parallel fiber to Purkinje cell synapse
Cellular Component
Plasma membrane
Cellular Component
Postsynapse
Cellular Component
Postsynaptic density
Cellular Component
Postsynaptic membrane
Cellular Component
Presynaptic membrane
Cellular Component
Schaffer collateral - CA1 synapse
Cellular Component
Synapse
Cellular Component
Synaptic vesicle
Cellular Component
Vesicle
Molecular Function
1-phosphatidylinositol binding
Molecular Function
Clathrin binding
Molecular Function
Clathrin heavy chain binding
Molecular Function
Identical protein binding
Molecular Function
Phosphatidylinositol-4,5-bisphosphate binding
Molecular Function
SH3 domain binding
Molecular Function
SNARE binding
Biological Process
Axonogenesis
Biological Process
Clathrin coat assembly
Biological Process
Clathrin-dependent endocytosis
Biological Process
Dendrite morphogenesis
Biological Process
Endocytosis
Biological Process
Iron ion homeostasis
Biological Process
Iron ion import across plasma membrane
Biological Process
Negative regulation of gene expression
Biological Process
Negative regulation of metalloendopeptidase activity involved in amyloid precursor protein catabolic process
Biological Process
Positive regulation of amyloid-beta formation
Biological Process
Positive regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process
Biological Process
Positive regulation of axonogenesis
Biological Process
Positive regulation of dendrite extension
Biological Process
Positive regulation of neuron death
Biological Process
Positive regulation of synaptic vesicle clustering
Biological Process
Positive regulation of synaptic vesicle endocytosis
Biological Process
Receptor-mediated endocytosis
Biological Process
Regulation of aspartic-type endopeptidase activity involved in amyloid precursor protein catabolic process
Biological Process
Regulation of endocytosis
Biological Process
Regulation of protein transport
Biological Process
Regulation of synaptic vesicle endocytosis
Biological Process
Regulation of synaptic vesicle transport
Biological Process
Regulation of terminal button organization
Biological Process
Synaptic vesicle budding from presynaptic endocytic zone membrane
Biological Process
Synaptic vesicle endocytosis
Biological Process
Synaptic vesicle maturation
Biological Process
Vesicle budding from membrane
Biological Process
Vesicle cargo loading
Biological Process
Vesicle-mediated transport

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Mum-3294Mus musculus98.180.01307
LLPS-Eqc-0082Equus caballus97.850.01294
LLPS-Loa-4327Loxodonta africana97.410.01290
LLPS-Ict-3626Ictidomys tridecemlineatus97.270.01297
LLPS-Mal-2009Mandrillus leucophaeus97.260.01287
LLPS-Paa-3335Papio anubis97.260.01287
LLPS-Maf-0457Macaca fascicularis97.260.01290
LLPS-Cea-3366Cercocebus atys97.260.01290
LLPS-Mam-0942Macaca mulatta97.260.01287
LLPS-Mup-2462Mustela putorius furo97.120.01294
LLPS-Pat-2942Pan troglodytes97.110.01286
LLPS-Pap-3013Pan paniscus97.110.01286
LLPS-Man-4563Macaca nemestrina97.110.01287
LLPS-Fud-2005Fukomys damarensis96.970.01298
LLPS-Gog-1693Gorilla gorilla96.960.01284
LLPS-Poa-4124Pongo abelii96.960.01285
LLPS-Orc-2355Oryctolagus cuniculus96.960.01288
LLPS-Nol-3394Nomascus leucogenys96.920.01271
LLPS-Mea-4230Mesocricetus auratus96.850.01041
LLPS-Cap-3173Cavia porcellus96.820.01296
LLPS-Ova-4083Ovis aries96.650.01224
LLPS-Bot-1405Bos taurus96.50.01282
LLPS-Caj-2264Callithrix jacchus96.140.01191
LLPS-Hos-1582Homo sapiens95.910.01268
LLPS-Aon-2027Aotus nancymaae95.750.01266
LLPS-Otg-0637Otolemur garnettii95.020.01246
LLPS-Caf-4089Canis familiaris94.840.01235
LLPS-Myl-3376Myotis lucifugus94.520.01253
LLPS-Sus-4054Sus scrofa93.60.01281
LLPS-Fec-3109Felis catus93.130.01268
LLPS-Fia-0603Ficedula albicollis93.120.01234
LLPS-Tag-3417Taeniopygia guttata92.270.01239
LLPS-Dio-3960Dipodomys ordii91.170.01207
LLPS-Mod-3193Monodelphis domestica90.640.01223
LLPS-Sah-3538Sarcophilus harrisii90.450.01058
LLPS-Rhb-3436Rhinopithecus bieti90.250.01167
LLPS-Ora-2458Ornithorhynchus anatinus89.610.01150
LLPS-Gaga-3918Gallus gallus87.270.01146
LLPS-Pes-3319Pelodiscus sinensis84.740.01026
LLPS-Anc-2492Anolis carolinensis83.80.01169
LLPS-Leo-3547Lepisosteus oculatus82.012e-171 520
LLPS-Dar-0130Danio rerio77.230.01036
LLPS-Asm-2670Astyanax mexicanus75.80.01051
LLPS-Icp-0240Ictalurus punctatus74.670.0 984
LLPS-Gaa-2435Gasterosteus aculeatus73.018e-153 471
LLPS-Pof-2381Poecilia formosa71.230.0 972
LLPS-Orn-3889Oreochromis niloticus70.860.0 890
LLPS-Orl-1955Oryzias latipes70.810.0 971
LLPS-Scm-1263Scophthalmus maximus70.670.0 955
LLPS-Tar-1162Takifugu rubripes67.980.0 840
LLPS-Aim-3012Ailuropoda melanoleuca66.679e-118 359
LLPS-Anp-3041Anas platyrhynchos64.34e-174 526
LLPS-Scf-2951Scleropages formosus61.390.0 782
LLPS-Ten-2722Tetraodon nigroviridis61.350.0 742
LLPS-Meg-2548Meleagris gallopavo60.280.0 684
LLPS-Xet-3287Xenopus tropicalis60.240.0 735
LLPS-Xim-1211Xiphophorus maculatus59.530.0 742
LLPS-Lac-0847Latimeria chalumnae58.240.0 558
LLPS-Tum-0269Tuber melanosporum34.881e-36 150
LLPS-Asc-1499Aspergillus clavatus33.334e-30 129
LLPS-Scc-1035Schizosaccharomyces cryophilus32.211e-30 130
LLPS-Abg-1372Absidia glauca32.124e-32 133
LLPS-Scj-0527Schizosaccharomyces japonicus32.091e-32 136
LLPS-Aso-0728Aspergillus oryzae32.061e-29 128
LLPS-Asf-0129Aspergillus flavus32.061e-29 128
LLPS-Nef-1082Neosartorya fischeri32.068e-31 132
LLPS-Ast-1257Aspergillus terreus31.951e-29 128
LLPS-Lem-1299Leptosphaeria maculans31.879e-35 144
LLPS-Asfu-0374Aspergillus fumigatus31.683e-30 130
LLPS-Gag-1174Gaeumannomyces graminis31.582e-27 122
LLPS-Mao-0400Magnaporthe oryzae31.491e-30 131
LLPS-Fuv-1487Fusarium verticillioides31.23e-30 130
LLPS-Map-0833Magnaporthe poae31.22e-26 118
LLPS-Fuo-1434Fusarium oxysporum31.23e-30 130
LLPS-Trv-1613Trichoderma virens31.25e-29 126
LLPS-Cog-1060Colletotrichum gloeosporioides31.25e-29 126
LLPS-Fus-1335Fusarium solani31.26e-30 129
LLPS-Cogr-1441Colletotrichum graminicola30.837e-29 126
LLPS-Miv-0166Microbotryum violaceum30.716e-27 120
LLPS-Asn-1220Aspergillus nidulans30.688e-28 122
LLPS-Nec-0230Neurospora crassa30.676e-31 132
LLPS-Beb-0785Beauveria bassiana30.454e-28 124
LLPS-Crn-1163Cryptococcus neoformans30.368e-32 136
LLPS-Coo-0149Colletotrichum orbiculare30.347e-28 123
LLPS-Blg-0117Blumeria graminis30.242e-30 130
LLPS-Asni-0749Aspergillus niger30.237e-30 129
LLPS-Asg-1372Ashbya gossypii30.123e-33 137
LLPS-Phn-1282Phaeosphaeria nodorum29.78e-30 129
LLPS-Trr-0458Trichoderma reesei29.393e-29 127
LLPS-Mel-0806Melampsora laricipopulina29.344e-23 107
LLPS-Pyt-0692Pyrenophora teres29.321e-30 131
LLPS-Pytr-1131Pyrenophora triticirepentis29.327e-31 132
LLPS-Scp-1346Schizosaccharomyces pombe29.293e-30 130
LLPS-Put-1316Puccinia triticina29.141e-25 116
LLPS-Nia-1260Nicotiana attenuata29.093e-29 127
LLPS-Sem-0050Selaginella moellendorffii29.069e-35 142
LLPS-Ved-1364Verticillium dahliae29.04e-29 127
LLPS-Coc-1717Corchorus capsularis28.848e-28 122
LLPS-Pug-1159Puccinia graminis28.811e-24 114
LLPS-Brd-0976Brachypodium distachyon28.787e-30 128
LLPS-Dos-1422Dothistroma septosporum28.682e-26 119
LLPS-Arl-2181Arabidopsis lyrata28.672e-28 124
LLPS-Kop-1418Komagataella pastoris28.621e-28 125
LLPS-Php-1000Physcomitrella patens28.372e-35 145
LLPS-Sot-0084Solanum tuberosum28.262e-30 130
LLPS-Mua-0442Musa acuminata27.941e-31 134
LLPS-Gor-1782Gossypium raimondii27.942e-30 130
LLPS-Pot-2378Populus trichocarpa27.946e-31 131
LLPS-Mae-2369Manihot esculenta27.944e-32 135
LLPS-Brn-3267Brassica napus27.95e-30 130
LLPS-Orb-1510Oryza barthii27.93e-27 120
LLPS-Orni-1658Oryza nivara27.93e-27 120
LLPS-Orr-1546Oryza rufipogon27.93e-27 120
LLPS-Bro-2339Brassica oleracea27.96e-30 130
LLPS-Brr-2414Brassica rapa27.86e-30 130
LLPS-Cus-0339Cucumis sativus27.572e-30 130
LLPS-Glm-0500Glycine max27.571e-30 130
LLPS-Thc-0368Theobroma cacao27.572e-30 130
LLPS-Prp-1317Prunus persica27.579e-31 131
LLPS-Art-2406Arabidopsis thaliana27.574e-29 127
LLPS-Viv-2316Vitis vinifera27.573e-30 129
LLPS-Phv-0396Phaseolus vulgaris27.478e-29 125
LLPS-Sac-1118Saccharomyces cerevisiae27.414e-24 111
LLPS-Usm-1312Ustilago maydis27.253e-26 119
LLPS-Via-1561Vigna angularis27.212e-30 130
LLPS-Sol-2447Solanum lycopersicum27.219e-28 121
LLPS-Hea-0987Helianthus annuus27.212e-26 118
LLPS-Ori-1672Oryza indica27.114e-26 117
LLPS-Spr-0666Sporisorium reilianum27.031e-25 117
LLPS-Dac-2353Daucus carota26.846e-29 125
LLPS-Tru-1369Triticum urartu26.796e-24 110
LLPS-Amt-0415Amborella trichopoda26.742e-28 124
LLPS-Sob-1864Sorghum bicolor26.241e-28 125
LLPS-Sei-1750Setaria italica26.17e-29 126
LLPS-Vir-0680Vigna radiata25.743e-25 114
LLPS-Tra-2854Triticum aestivum25.362e-27 121
LLPS-Osl-1473Ostreococcus lucimarinus25.32e-20 100
LLPS-Met-1076Medicago truncatula25.191e-26 119
LLPS-Hov-1139Hordeum vulgare25.02e-27 121
LLPS-Orm-1671Oryza meridionalis25.03e-27 120
LLPS-Orp-0348Oryza punctata25.03e-27 120
LLPS-Org-0097Oryza glaberrima24.915e-26 117
LLPS-Ors-0177Oryza sativa24.914e-26 117
LLPS-Orbr-2287Oryza brachyantha24.916e-27 120
LLPS-Zem-2098Zea mays24.632e-27 120
LLPS-Lep-0628Leersia perrieri24.547e-27 119
LLPS-Gas-1310Galdieria sulphuraria24.186e-28 123