DrLLPS - Data resource of LLPS

LLPS-Brr-1004

Integrated Annotations

▼ OVERVIEW


Status:	Unreviewed
Protein Name:	ATPase; Arsenical pump-driving ATPase; Arsenite-stimulated ATPase
Ensembl Gene:	Bra032654
Ensembl Protein:	Bra032654.1-P
Organism:	Brassica rapa
Taxa ID:	51351
LLPS Type:	Client

▼ PROPERTY

——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:

Condensate	Evidence	Orthologs
Nucleolus	Predicted from orthologs	(View)

Organism	DrLLPS ID	UniProt ID	LLPS type	Condensate	Identity	E-value	Score
Arabidopsis thaliana	LLPS-Art-0584	Q949M9	Client	Nucleolus	95.45	0.0	646

▼ FUNCTION

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting.

▼ CROSS REFERENCE

Database	Nucleotide ID	Protein ID
Ensembl	Bra032654.1	Bra032654.1-P
UniProt		M4EV21, M4EV21_BRARP

▼ SEQUENCE

Protein Sequence (FASTA)
1 MASDMPEATV QNILDQDSLK WVFVGGKGGV GKTTCSSILS ICLARVRSSV LIISTDPAHN 60 61 LSDAFQQRFT KSPTLVQGFS NLFAMEVDPT VETDDLAGPE GMDSLFSDLA NAIPGIDEAM 120 121 SFAEMLKLVQ TMDYATIVFD TAPTGHTLRL LQFPATLEKG LSKLMSLKSR FAGFVLQMSR 180 181 MFGMEDEFGE DALLGRLEGL KDVIEQVNRQ FKDPDLTTFV CVCIPEFLSL YETERLVQEL 240 241 AKFEIDTHNI IINQVLYDDE DVESKLLRAR MRMQQKYLDQ FYMLYDDFNI TKLPLLPEEV 300 301 TGVEALKAFS DKFLTPYHPT TSRSNVEELE RKVHTLRLQL KTAEEELERI KS 352
Nucleotide CDS Sequence (FASTA)
1 ATGGCGTCGG ATATGCCGGA GGCGACAGTG CAGAACATTC TGGATCAAGA TTCTCTAAAA 60 61 TGGGTATTCG TGGGTGGGAA AGGAGGCGTT GGGAAGACCA CATGCAGTTC CATCCTCTCT 120 121 ATTTGTCTCG CTAGGGTTCG ATCCTCCGTC CTCATCATCT CCACCGACCC CGCTCACAAT 180 181 CTCAGCGACG CCTTTCAGCA GCGCTTCACT AAATCCCCCA CTTTGGTTCA AGGCTTCTCC 240 241 AATCTCTTTG CCATGGAGGT GGATCCTACA GTTGAAACTG ATGACTTGGC TGGCCCTGAA 300 301 GGGATGGATA GTTTATTCTC TGATTTGGCC AATGCCATTC CTGGTATCGA TGAGGCCATG 360 361 AGTTTCGCTG AGATGCTCAA GTTGGTCCAA ACTATGGATT ATGCAACTAT TGTCTTTGAC 420 421 ACCGCTCCTA CTGGTCACAC TCTCCGTCTC TTGCAGTTCC CCGCCACTCT TGAGAAGGGC 480 481 CTTTCCAAAT TGATGTCCTT GAAGAGTCGC TTTGCTGGTT TCGTTTTGCA GATGAGCCGT 540 541 ATGTTTGGCA TGGAAGATGA GTTTGGGGAG GATGCTCTCT TGGGAAGACT TGAGGGCTTG 600 601 AAGGATGTCA TTGAACAAGT TAATCGCCAG TTTAAAGACC CGGATTTGAC AACGTTCGTC 660 661 TGTGTCTGCA TCCCTGAGTT CTTGTCTCTC TATGAAACGG AGAGATTAGT TCAGGAACTC 720 721 GCAAAGTTTG AGATCGACAC GCATAACATC ATCATCAACC AAGTACTCTA CGATGACGAA 780 781 GATGTGGAAT CCAAGTTGCT CAGAGCAAGG ATGCGAATGC AGCAGAAGTA CCTTGATCAG 840 841 TTTTATATGC TATACGATGA CTTCAATATC ACAAAACTTC CTCTGCTTCC CGAAGAGGTG 900 901 ACAGGGGTGG AAGCGTTAAA GGCATTCTCG GATAAGTTCT TGACGCCGTA CCATCCAACG 960 961 ACAAGCAGAA GCAATGTAGA AGAGCTGGAG AGGAAAGTAC ACACATTGCG TCTGCAGTTG 1020 1021 AAAACAGCGG AAGAAGAACT GGAACGGATC AAGAGTTGA 1059

▼ KEYWORD

ID	Family
KW-0067	ATP-binding
KW-0175	Coiled coil
KW-0181	Complete proteome
KW-0963	Cytoplasm
KW-0256	Endoplasmic reticulum
KW-0378	Hydrolase
KW-0547	Nucleotide-binding
KW-1185	Reference proteome
KW-0813	Transport

▼ GENE ONTOLOGY

ID	Classification	Description
GO:0005783	Cellular Component	Endoplasmic reticulum
GO:0005524	Molecular Function	ATP binding
GO:0016887	Molecular Function	ATPase activity
GO:0045048	Biological Process	Protein insertion into ER membrane

▼ ORTHOLOGY

DrLLPS ID	Organism	Identity	E-value	Score
LLPS-Brn-2437	Brassica napus	98.58	0.0	686
LLPS-Bro-1581	Brassica oleracea	98.58	0.0	686
LLPS-Art-0584	Arabidopsis thaliana	95.45	0.0	669
LLPS-Arl-2700	Arabidopsis lyrata	95.17	0.0	667
LLPS-Thc-1923	Theobroma cacao	83.91	0.0	590
LLPS-Cus-1618	Cucumis sativus	82.91	0.0	584
LLPS-Gor-2554	Gossypium raimondii	82.76	0.0	590
LLPS-Prp-1361	Prunus persica	82.29	0.0	580
LLPS-Coc-1857	Corchorus capsularis	81.32	0.0	580
LLPS-Pot-2307	Populus trichocarpa	81.03	0.0	577
LLPS-Viv-0748	Vitis vinifera	80.97	0.0	564
LLPS-Hea-0701	Helianthus annuus	80.46	0.0	565
LLPS-Glm-1760	Glycine max	80.34	0.0	562
LLPS-Amt-1299	Amborella trichopoda	80.06	0.0	556
LLPS-Mae-1169	Manihot esculenta	79.94	0.0	553
LLPS-Met-0637	Medicago truncatula	79.89	0.0	562
LLPS-Sot-0920	Solanum tuberosum	78.87	0.0	564
LLPS-Phv-2501	Phaseolus vulgaris	78.57	0.0	550
LLPS-Nia-0472	Nicotiana attenuata	78.45	0.0	560
LLPS-Brd-1007	Brachypodium distachyon	76.57	0.0	538
LLPS-Dac-0723	Daucus carota	76.06	6e-109	325
LLPS-Sei-1427	Setaria italica	76.0	0.0	538
LLPS-Zem-1520	Zea mays	76.0	0.0	535
LLPS-Orni-0900	Oryza nivara	75.5	0.0	528
LLPS-Ori-2105	Oryza indica	75.5	0.0	528
LLPS-Orb-1473	Oryza barthii	75.5	0.0	528
LLPS-Org-0807	Oryza glaberrima	75.5	0.0	528
LLPS-Orr-2345	Oryza rufipogon	75.5	0.0	528
LLPS-Ors-1105	Oryza sativa	75.5	0.0	528
LLPS-Orp-1004	Oryza punctata	75.5	0.0	528
LLPS-Lep-1440	Leersia perrieri	75.43	0.0	534
LLPS-Orgl-2022	Oryza glumaepatula	75.22	0.0	526
LLPS-Orm-0503	Oryza meridionalis	75.22	0.0	527
LLPS-Tra-1944	Triticum aestivum	75.14	0.0	532
LLPS-Hov-2065	Hordeum vulgare	75.14	0.0	532
LLPS-Sob-0732	Sorghum bicolor	75.14	0.0	532
LLPS-Orbr-1778	Oryza brachyantha	74.86	0.0	526
LLPS-Tru-1195	Triticum urartu	72.93	1e-165	475
LLPS-Mua-0307	Musa acuminata	71.7	0.0	522
LLPS-Php-2002	Physcomitrella patens	69.7	5e-173	493
LLPS-Sem-1308	Selaginella moellendorffii	69.25	1e-160	461
LLPS-Vir-1467	Vigna radiata	67.16	1e-178	509
LLPS-Chr-1484	Chlamydomonas reinhardtii	60.66	6e-133	391
LLPS-Abg-1398	Absidia glauca	56.74	1e-117	351
LLPS-Usm-1104	Ustilago maydis	56.31	4e-109	329
LLPS-Crn-0734	Cryptococcus neoformans	56.25	3e-112	337
LLPS-Miv-0064	Microbotryum violaceum	56.21	1e-110	333
LLPS-Tum-0519	Tuber melanosporum	56.15	3e-112	337
LLPS-Phn-0018	Phaeosphaeria nodorum	56.11	2e-104	317
LLPS-Mel-0048	Melampsora laricipopulina	56.06	3e-114	342
LLPS-Pug-0991	Puccinia graminis	55.86	2e-109	330
LLPS-Tar-1146	Takifugu rubripes	55.76	3e-114	343
LLPS-Scc-1628	Schizosaccharomyces cryophilus	55.76	2e-108	327
LLPS-Tut-2481	Tursiops truncatus	55.7	2e-103	314
LLPS-Drm-0597	Drosophila melanogaster	55.69	3e-113	340
LLPS-Spr-1255	Sporisorium reilianum	55.69	1e-107	325
LLPS-Blg-1586	Blumeria graminis	55.59	1e-110	333
LLPS-Asm-2538	Astyanax mexicanus	55.45	3e-113	340
LLPS-Orl-1120	Oryzias latipes	55.45	1e-113	341
LLPS-Scm-3613	Scophthalmus maximus	55.45	1e-113	341
LLPS-Dar-1539	Danio rerio	55.45	1e-113	341
LLPS-Leo-3897	Lepisosteus oculatus	55.42	3e-114	342
LLPS-Pof-3321	Poecilia formosa	55.28	3e-112	337
LLPS-Pyt-0964	Pyrenophora teres	55.25	7e-109	329
LLPS-Pytr-0927	Pyrenophora triticirepentis	55.25	4e-109	329
LLPS-Scs-0905	Sclerotinia sclerotiorum	55.18	5e-111	334
LLPS-Xim-0032	Xiphophorus maculatus	55.14	6e-113	339
LLPS-Icp-2882	Ictalurus punctatus	55.14	9e-113	338
LLPS-Orn-3402	Oreochromis niloticus	55.08	3e-114	343
LLPS-Put-0585	Puccinia triticina	54.94	2e-107	325
LLPS-Scj-1028	Schizosaccharomyces japonicus	54.89	6e-109	328
LLPS-Scf-2649	Scleropages formosus	54.83	2e-113	340
LLPS-Lac-0814	Latimeria chalumnae	54.8	1e-113	341
LLPS-Myl-3705	Myotis lucifugus	54.7	9e-53	179
LLPS-Cog-1445	Colletotrichum gloeosporioides	54.68	3e-109	330
LLPS-Cii-1640	Ciona intestinalis	54.66	8e-111	334
LLPS-Beb-0241	Beauveria bassiana	54.52	2e-102	312
LLPS-Anc-3834	Anolis carolinensis	54.46	1e-113	341
LLPS-Cap-4015	Cavia porcellus	54.43	5e-112	337
LLPS-Lem-0728	Leptosphaeria maculans	54.35	8e-108	328
LLPS-Map-1333	Magnaporthe poae	54.33	3e-104	317
LLPS-Asc-0362	Aspergillus clavatus	54.27	2e-108	327
LLPS-Nef-0553	Neosartorya fischeri	54.24	9e-108	326
LLPS-Ran-1852	Rattus norvegicus	54.15	1e-111	336
LLPS-Mup-0229	Mustela putorius furo	54.15	1e-111	336
LLPS-Aim-2872	Ailuropoda melanoleuca	54.15	1e-111	336
LLPS-Ova-2011	Ovis aries	54.15	5e-112	337
LLPS-Mal-2189	Mandrillus leucophaeus	54.15	1e-111	336
LLPS-Paa-0671	Papio anubis	54.15	1e-111	336
LLPS-Bot-1334	Bos taurus	54.15	1e-111	336
LLPS-Dio-4069	Dipodomys ordii	54.15	2e-111	335
LLPS-Caj-1802	Callithrix jacchus	54.15	1e-111	336
LLPS-Fud-3396	Fukomys damarensis	54.15	1e-111	336
LLPS-Chs-0873	Chlorocebus sabaeus	54.15	7e-112	337
LLPS-Maf-1555	Macaca fascicularis	54.15	1e-111	336
LLPS-Mum-3112	Mus musculus	54.15	1e-111	336
LLPS-Sus-4227	Sus scrofa	54.15	1e-111	336
LLPS-Cea-2200	Cercocebus atys	54.15	1e-111	336
LLPS-Sah-2036	Sarcophilus harrisii	54.15	8e-112	337
LLPS-Aon-3694	Aotus nancymaae	54.15	1e-111	336
LLPS-Cas-2750	Carlito syrichta	54.15	1e-111	336
LLPS-Ict-3904	Ictidomys tridecemlineatus	54.15	1e-111	336
LLPS-Poa-1666	Pongo abelii	54.15	1e-111	336
LLPS-Mam-0930	Macaca mulatta	54.15	9e-112	337
LLPS-Otg-4345	Otolemur garnettii	54.15	1e-111	336
LLPS-Rhb-4762	Rhinopithecus bieti	54.15	1e-111	336
LLPS-Fec-0392	Felis catus	54.15	1e-111	336
LLPS-Hos-4280	Homo sapiens	54.15	1e-111	336
LLPS-Urm-3504	Ursus maritimus	54.15	1e-111	336
LLPS-Pap-2114	Pan paniscus	54.15	1e-111	336
LLPS-Loa-2699	Loxodonta africana	54.15	1e-111	336
LLPS-Man-0873	Macaca nemestrina	54.15	1e-111	336
LLPS-Cis-1536	Ciona savignyi	54.1	7e-112	337
LLPS-Coo-1560	Colletotrichum orbiculare	54.08	1e-106	323
LLPS-Cogr-0829	Colletotrichum graminicola	54.08	5e-109	329
LLPS-Fus-1086	Fusarium solani	54.08	1e-106	323
LLPS-Trv-1259	Trichoderma virens	54.05	9e-107	323
LLPS-Asn-1200	Aspergillus nidulans	53.96	3e-107	325
LLPS-Nec-0933	Neurospora crassa	53.94	5e-105	319
LLPS-Gog-1932	Gorilla gorilla	53.85	2e-110	333
LLPS-Eqc-3853	Equus caballus	53.85	4e-111	335
LLPS-Mod-4085	Monodelphis domestica	53.85	2e-111	335
LLPS-Ved-1188	Verticillium dahliae	53.78	3e-107	325
LLPS-Gag-1594	Gaeumannomyces graminis	53.73	1e-106	323
LLPS-Ast-1342	Aspergillus terreus	53.66	3e-107	325
LLPS-Aso-0103	Aspergillus oryzae	53.64	6e-108	326
LLPS-Asni-1552	Aspergillus niger	53.64	1e-107	326
LLPS-Asfu-1511	Aspergillus fumigatus	53.64	2e-107	325
LLPS-Mao-0871	Magnaporthe oryzae	53.45	1e-105	320
LLPS-Scp-1378	Schizosaccharomyces pombe	53.17	1e-107	325
LLPS-Fuv-0546	Fusarium verticillioides	53.13	1e-105	321
LLPS-Fuo-1050	Fusarium oxysporum	53.13	1e-105	321
LLPS-Gaa-0555	Gasterosteus aculeatus	53.08	3e-104	315
LLPS-Asf-0682	Aspergillus flavus	53.05	2e-102	314
LLPS-Trr-0356	Trichoderma reesei	52.99	2e-105	320
LLPS-Nol-1743	Nomascus leucogenys	52.97	2e-78	248
LLPS-Pat-4113	Pan troglodytes	52.31	3e-109	330
LLPS-Yal-1554	Yarrowia lipolytica	52.15	1e-103	315
LLPS-Ora-0424	Ornithorhynchus anatinus	52.03	6e-104	315
LLPS-Dos-1287	Dothistroma septosporum	51.35	6e-100	306
LLPS-Zyt-0613	Zymoseptoria tritici	51.2	3e-100	306
LLPS-Caf-4175	Canis familiaris	50.46	4e-98	301
LLPS-Cae-1581	Caenorhabditis elegans	50.45	1e-97	300
LLPS-Xet-1105	Xenopus tropicalis	50.15	1e-100	308
LLPS-Ten-2250	Tetraodon nigroviridis	49.54	1e-84	266
LLPS-Chc-0676	Chondrus crispus	47.27	3e-81	267
LLPS-Kop-1233	Komagataella pastoris	46.8	1e-87	275
LLPS-Gas-0745	Galdieria sulphuraria	46.29	8e-81	267
LLPS-Gaga-3173	Gallus gallus	46.08	5e-83	262
LLPS-Asg-1486	Ashbya gossypii	43.27	9e-83	262
LLPS-Sac-1375	Saccharomyces cerevisiae	41.69	3e-77	248
LLPS-Cym-0841	Cyanidioschyzon merolae	40.77	7e-71	241
LLPS-Orc-4050	Oryctolagus cuniculus	32.81	3e-34	134

LLPS-Brr-1004

Protein Sequence

Nucleotide Sequence