LLPS-Asg-1486
GET3
Integrated Annotations
▼ OVERVIEW
| Status: | Unreviewed |
| Protein Name: | ATPase GET3; Arsenical pump-driving ATPase; Arsenite-stimulated ATPase; Golgi to ER traffic protein 3; Guided entry of tail-anchored proteins 3 |
| Gene Name: | GET3, ADR285W |
| Ensembl Gene: | AGOS_ADR285W |
| Ensembl Protein: | AAS52205 |
| Organism: | Ashbya gossypii |
| Taxa ID: | 284811 |
| LLPS Type: | Others |
▼ Classification
Condensates:
| Condensate | Evidence | Orthologs |
|---|---|---|
| Nucleolus | Predicted from orthologs | (View) |
▼ FUNCTION
| ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | AAS52205 | AAS52205 |
| UniProt | Q759J2, GET3_ASHGO | |
| GeneBank | AE016817 | AAS52205.2 |
| RefSeq | NM_209734.2 | NP_984381.2 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MTDITPEASL RSLINSTTHK WIFVGGKGGV GKTTSSCSIA IQMALAQPTK QFLLISTDPA 60 61 HNLSDAFNEK FGKDARKVTG MDNLSCMEID PSAALKDVND MAIANGGDDD GLSGLLQGGA 120 121 LADLTGSIPG IDEALSFMEV MKHIKKQEQG DGEHFDTVIF DTAPTGHTLR FLQLPTTLTK 180 181 VLDKFGAIAG RLGPMLNSFA GNPNVDVLGK MNELKESVQK IKKQFTDPDL TTFVCVCISE 240 241 FLSLYETERL IQELISYDMD VNSIIVNQLL FAESDKEHNC KRCQARWKMQ KKYLSQIDEL 300 301 YEDFHIVKMP LCAGEIRGLE NLKKFSCFLN NKYDPETDGE LVYQLEQGL 349 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGACTGATA TTACACCAGA GGCCTCTTTG CGCTCGTTGA TCAACTCTAC AACACACAAG 60 61 TGGATTTTCG TCGGCGGGAA GGGCGGTGTG GGCAAGACCA CGTCTTCGTG CTCCATTGCC 120 121 ATCCAGATGG CGCTTGCGCA GCCCACTAAG CAGTTTCTTT TGATCTCGAC GGATCCTGCG 180 181 CATAACCTTA GCGATGCGTT CAACGAGAAG TTTGGGAAGG ACGCGCGCAA GGTCACGGGC 240 241 ATGGACAACT TGTCGTGTAT GGAAATCGAC CCTTCGGCGG CGTTGAAGGA CGTGAACGAC 300 301 ATGGCGATCG CGAACGGCGG CGATGATGAC GGGCTCTCGG GCTTGCTACA GGGCGGCGCA 360 361 CTCGCAGATC TCACGGGGTC GATTCCGGGC ATCGACGAGG CGCTTTCGTT CATGGAGGTG 420 421 ATGAAACACA TCAAGAAGCA GGAGCAGGGC GACGGCGAGC ACTTCGACAC GGTGATCTTT 480 481 GACACCGCGC CCACGGGCCA TACGTTGCGC TTCTTGCAGC TCCCAACTAC GCTCACGAAG 540 541 GTGCTAGACA AGTTTGGCGC CATTGCAGGC CGCCTTGGGC CGATGCTCAA CTCTTTTGCG 600 601 GGCAACCCCA ACGTCGACGT GCTTGGCAAG ATGAACGAAC TCAAGGAAAG CGTCCAGAAG 660 661 ATCAAAAAGC AGTTCACCGA TCCGGACTTG ACGACCTTTG TTTGTGTCTG TATCAGCGAG 720 721 TTCTTATCGC TCTACGAAAC CGAGCGGCTC ATCCAAGAGC TTATCTCCTA CGACATGGAC 780 781 GTAAACTCCA TCATTGTGAA CCAGCTCCTA TTCGCGGAAA GCGACAAGGA GCACAATTGT 840 841 AAGCGCTGCC AAGCAAGATG GAAGATGCAG AAGAAGTACT TGTCCCAGAT TGACGAGTTG 900 901 TATGAGGACT TCCACATTGT CAAAATGCCA CTTTGCGCCG GCGAGATCCG CGGCTTGGAA 960 961 AACTTGAAGA AGTTCTCTTG CTTTTTGAAC AACAAATACG ACCCTGAAAC TGATGGCGAA 1020 1021 CTCGTCTACC AGTTGGAACA GGGATTATAG 1050 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | GET complex | |
Molecular Function | ATP binding | |
Molecular Function | ATPase activity | |
Molecular Function | Guanyl-nucleotide exchange factor activity | |
Molecular Function | Identical protein binding | |
Molecular Function | Metal ion binding | |
Molecular Function | Unfolded protein binding | |
Biological Process | ATP-independent chaperone mediated protein folding | |
Biological Process | Pheromone-dependent signal transduction involved in conjugation with cellular fusion | |
Biological Process | Posttranslational protein targeting to endoplasmic reticulum membrane | |
Biological Process | Response to heat | |
Biological Process | Response to metal ion | |
Biological Process | Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum | |
Biological Process | Tail-anchored membrane protein insertion into ER membrane |
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Sac-1375 | Saccharomyces cerevisiae | 81.14 | 0.0 | 532 |
| LLPS-Kop-1233 | Komagataella pastoris | 60.68 | 1e-134 | 395 |
| LLPS-Asni-1552 | Aspergillus niger | 56.68 | 4e-107 | 324 |
| LLPS-Asf-0682 | Aspergillus flavus | 55.83 | 3e-102 | 314 |
| LLPS-Ast-1342 | Aspergillus terreus | 55.49 | 4e-106 | 322 |
| LLPS-Asn-1200 | Aspergillus nidulans | 55.49 | 3e-106 | 322 |
| LLPS-Aso-0103 | Aspergillus oryzae | 55.49 | 2e-106 | 322 |
| LLPS-Asfu-1511 | Aspergillus fumigatus | 54.9 | 8e-105 | 318 |
| LLPS-Asc-0362 | Aspergillus clavatus | 54.9 | 8e-107 | 323 |
| LLPS-Scp-1378 | Schizosaccharomyces pombe | 54.73 | 1e-106 | 323 |
| LLPS-Pytr-0927 | Pyrenophora triticirepentis | 54.68 | 1e-104 | 318 |
| LLPS-Scj-1028 | Schizosaccharomyces japonicus | 54.68 | 3e-103 | 314 |
| LLPS-Pyt-0964 | Pyrenophora teres | 54.68 | 2e-104 | 317 |
| LLPS-Nef-0553 | Neosartorya fischeri | 54.6 | 1e-104 | 318 |
| LLPS-Blg-1586 | Blumeria graminis | 54.55 | 7e-105 | 318 |
| LLPS-Fus-1086 | Fusarium solani | 54.44 | 3e-102 | 311 |
| LLPS-Lem-0728 | Leptosphaeria maculans | 54.23 | 3e-103 | 317 |
| LLPS-Cog-1445 | Colletotrichum gloeosporioides | 54.01 | 2e-101 | 309 |
| LLPS-Phn-0018 | Phaeosphaeria nodorum | 53.92 | 9e-101 | 307 |
| LLPS-Beb-0241 | Beauveria bassiana | 53.85 | 1e-99 | 305 |
| LLPS-Trv-1259 | Trichoderma virens | 53.85 | 2e-101 | 310 |
| LLPS-Fuv-0546 | Fusarium verticillioides | 53.85 | 1e-100 | 308 |
| LLPS-Cogr-0829 | Colletotrichum graminicola | 53.71 | 2e-102 | 312 |
| LLPS-Fuo-1050 | Fusarium oxysporum | 53.55 | 5e-100 | 306 |
| LLPS-Scc-1628 | Schizosaccharomyces cryophilus | 53.47 | 8e-102 | 310 |
| LLPS-Trr-0356 | Trichoderma reesei | 53.25 | 3e-99 | 304 |
| LLPS-Miv-0064 | Microbotryum violaceum | 52.99 | 6e-100 | 306 |
| LLPS-Coo-1560 | Colletotrichum orbiculare | 52.92 | 5e-102 | 311 |
| LLPS-Scs-0905 | Sclerotinia sclerotiorum | 52.79 | 3e-100 | 306 |
| LLPS-Map-1333 | Magnaporthe poae | 52.66 | 2e-100 | 307 |
| LLPS-Ved-1188 | Verticillium dahliae | 52.52 | 3e-102 | 312 |
| LLPS-Yal-1554 | Yarrowia lipolytica | 52.52 | 2e-102 | 311 |
| LLPS-Nec-0933 | Neurospora crassa | 52.52 | 5e-98 | 301 |
| LLPS-Tum-0519 | Tuber melanosporum | 52.21 | 4e-103 | 313 |
| LLPS-Mao-0871 | Magnaporthe oryzae | 52.2 | 5e-100 | 306 |
| LLPS-Zyt-0613 | Zymoseptoria tritici | 52.03 | 3e-102 | 311 |
| LLPS-Dos-1287 | Dothistroma septosporum | 51.46 | 8e-97 | 298 |
| LLPS-Mel-0048 | Melampsora laricipopulina | 51.03 | 3e-101 | 309 |
| LLPS-Gag-1594 | Gaeumannomyces graminis | 50.88 | 7e-100 | 306 |
| LLPS-Usm-1104 | Ustilago maydis | 50.44 | 3e-99 | 303 |
| LLPS-Abg-1398 | Absidia glauca | 50.44 | 8e-98 | 300 |
| LLPS-Pug-0991 | Puccinia graminis | 50.15 | 4e-99 | 303 |
| LLPS-Spr-1255 | Sporisorium reilianum | 50.15 | 2e-98 | 301 |
| LLPS-Put-0585 | Puccinia triticina | 49.85 | 8e-97 | 298 |
| LLPS-Crn-0734 | Cryptococcus neoformans | 49.71 | 2e-99 | 304 |
| LLPS-Cae-1581 | Caenorhabditis elegans | 47.98 | 1e-81 | 259 |
| LLPS-Nol-1743 | Nomascus leucogenys | 47.47 | 3e-61 | 203 |
| LLPS-Tut-2481 | Tursiops truncatus | 47.47 | 5e-78 | 249 |
| LLPS-Pof-3321 | Poecilia formosa | 46.92 | 4e-84 | 265 |
| LLPS-Leo-3897 | Lepisosteus oculatus | 46.49 | 9e-85 | 267 |
| LLPS-Scm-3613 | Scophthalmus maximus | 46.47 | 2e-84 | 266 |
| LLPS-Dar-1539 | Danio rerio | 46.47 | 2e-84 | 266 |
| LLPS-Asm-2538 | Astyanax mexicanus | 46.47 | 1e-84 | 266 |
| LLPS-Orl-1120 | Oryzias latipes | 46.47 | 4e-84 | 265 |
| LLPS-Tar-1146 | Takifugu rubripes | 46.47 | 3e-84 | 265 |
| LLPS-Xim-0032 | Xiphophorus maculatus | 46.47 | 1e-84 | 266 |
| LLPS-Gog-1932 | Gorilla gorilla | 46.45 | 5e-83 | 263 |
| LLPS-Otg-4345 | Otolemur garnettii | 46.2 | 4e-84 | 265 |
| LLPS-Rhb-4762 | Rhinopithecus bieti | 46.2 | 4e-84 | 265 |
| LLPS-Mod-4085 | Monodelphis domestica | 46.2 | 3e-84 | 266 |
| LLPS-Fec-0392 | Felis catus | 46.2 | 4e-84 | 265 |
| LLPS-Hos-4280 | Homo sapiens | 46.2 | 4e-84 | 265 |
| LLPS-Pap-2114 | Pan paniscus | 46.2 | 4e-84 | 265 |
| LLPS-Urm-3504 | Ursus maritimus | 46.2 | 4e-84 | 265 |
| LLPS-Loa-2699 | Loxodonta africana | 46.2 | 4e-84 | 265 |
| LLPS-Man-0873 | Macaca nemestrina | 46.2 | 4e-84 | 265 |
| LLPS-Cas-2750 | Carlito syrichta | 46.2 | 4e-84 | 265 |
| LLPS-Anc-3834 | Anolis carolinensis | 46.2 | 5e-84 | 265 |
| LLPS-Ict-3904 | Ictidomys tridecemlineatus | 46.2 | 4e-84 | 265 |
| LLPS-Cap-4015 | Cavia porcellus | 46.2 | 2e-84 | 266 |
| LLPS-Poa-1666 | Pongo abelii | 46.2 | 4e-84 | 265 |
| LLPS-Mam-0930 | Macaca mulatta | 46.2 | 4e-84 | 265 |
| LLPS-Caj-1802 | Callithrix jacchus | 46.2 | 4e-84 | 265 |
| LLPS-Fud-3396 | Fukomys damarensis | 46.2 | 3e-84 | 266 |
| LLPS-Chs-0873 | Chlorocebus sabaeus | 46.2 | 3e-84 | 266 |
| LLPS-Maf-1555 | Macaca fascicularis | 46.2 | 4e-84 | 265 |
| LLPS-Mum-3112 | Mus musculus | 46.2 | 4e-84 | 265 |
| LLPS-Sus-4227 | Sus scrofa | 46.2 | 4e-84 | 265 |
| LLPS-Aon-3694 | Aotus nancymaae | 46.2 | 4e-84 | 265 |
| LLPS-Cea-2200 | Cercocebus atys | 46.2 | 4e-84 | 265 |
| LLPS-Sah-2036 | Sarcophilus harrisii | 46.2 | 3e-84 | 266 |
| LLPS-Ran-1852 | Rattus norvegicus | 46.2 | 4e-84 | 265 |
| LLPS-Aim-2872 | Ailuropoda melanoleuca | 46.2 | 4e-84 | 265 |
| LLPS-Mup-0229 | Mustela putorius furo | 46.2 | 4e-84 | 265 |
| LLPS-Ova-2011 | Ovis aries | 46.2 | 6e-84 | 265 |
| LLPS-Mal-2189 | Mandrillus leucophaeus | 46.2 | 4e-84 | 265 |
| LLPS-Paa-0671 | Papio anubis | 46.2 | 4e-84 | 265 |
| LLPS-Bot-1334 | Bos taurus | 46.2 | 4e-84 | 265 |
| LLPS-Dio-4069 | Dipodomys ordii | 46.2 | 1e-83 | 264 |
| LLPS-Orn-3402 | Oreochromis niloticus | 46.18 | 2e-84 | 266 |
| LLPS-Eqc-3853 | Equus caballus | 46.18 | 2e-83 | 264 |
| LLPS-Icp-2882 | Ictalurus punctatus | 46.18 | 2e-84 | 266 |
| LLPS-Scf-2649 | Scleropages formosus | 46.18 | 4e-84 | 265 |
| LLPS-Cis-1536 | Ciona savignyi | 46.13 | 3e-80 | 256 |
| LLPS-Drm-0597 | Drosophila melanogaster | 46.13 | 2e-82 | 261 |
| LLPS-Php-2002 | Physcomitrella patens | 45.79 | 4e-82 | 261 |
| LLPS-Sem-1308 | Selaginella moellendorffii | 45.72 | 1e-73 | 239 |
| LLPS-Nia-0472 | Nicotiana attenuata | 45.69 | 5e-79 | 253 |
| LLPS-Hea-0701 | Helianthus annuus | 45.4 | 6e-78 | 250 |
| LLPS-Lac-0814 | Latimeria chalumnae | 45.32 | 9e-83 | 262 |
| LLPS-Amt-1299 | Amborella trichopoda | 45.16 | 1e-79 | 254 |
| LLPS-Gaa-0555 | Gasterosteus aculeatus | 44.92 | 7e-80 | 253 |
| LLPS-Cii-1640 | Ciona intestinalis | 44.91 | 1e-79 | 253 |
| LLPS-Chr-1484 | Chlamydomonas reinhardtii | 44.77 | 6e-79 | 253 |
| LLPS-Pat-4113 | Pan troglodytes | 44.74 | 2e-84 | 266 |
| LLPS-Ora-0424 | Ornithorhynchus anatinus | 44.63 | 3e-80 | 254 |
| LLPS-Sot-0920 | Solanum tuberosum | 44.51 | 5e-75 | 242 |
| LLPS-Pot-2307 | Populus trichocarpa | 44.41 | 7e-81 | 257 |
| LLPS-Thc-1923 | Theobroma cacao | 44.41 | 5e-82 | 260 |
| LLPS-Tra-1944 | Triticum aestivum | 44.38 | 6e-76 | 245 |
| LLPS-Glm-1760 | Glycine max | 44.31 | 1e-75 | 243 |
| LLPS-Zem-1520 | Zea mays | 44.07 | 2e-75 | 243 |
| LLPS-Chc-0676 | Chondrus crispus | 44.07 | 2e-69 | 236 |
| LLPS-Myl-3705 | Myotis lucifugus | 43.88 | 9e-34 | 129 |
| LLPS-Bro-1581 | Brassica oleracea | 43.84 | 3e-80 | 255 |
| LLPS-Coc-1857 | Corchorus capsularis | 43.84 | 2e-81 | 259 |
| LLPS-Brn-2437 | Brassica napus | 43.84 | 3e-80 | 255 |
| LLPS-Arl-2700 | Arabidopsis lyrata | 43.84 | 1e-80 | 256 |
| LLPS-Gor-2554 | Gossypium raimondii | 43.62 | 1e-79 | 254 |
| LLPS-Caf-4175 | Canis familiaris | 43.57 | 1e-75 | 243 |
| LLPS-Art-0584 | Arabidopsis thaliana | 43.55 | 9e-80 | 254 |
| LLPS-Lep-1440 | Leersia perrieri | 43.55 | 5e-78 | 250 |
| LLPS-Mua-0307 | Musa acuminata | 43.38 | 3e-81 | 259 |
| LLPS-Brr-1004 | Brassica rapa | 43.27 | 2e-77 | 248 |
| LLPS-Cus-1618 | Cucumis sativus | 43.22 | 4e-84 | 266 |
| LLPS-Prp-1361 | Prunus persica | 43.1 | 3e-79 | 254 |
| LLPS-Orp-1004 | Oryza punctata | 43.02 | 1e-76 | 247 |
| LLPS-Sob-0732 | Sorghum bicolor | 42.86 | 2e-76 | 246 |
| LLPS-Sei-1427 | Setaria italica | 42.86 | 1e-76 | 246 |
| LLPS-Met-0637 | Medicago truncatula | 42.82 | 2e-75 | 243 |
| LLPS-Orm-0503 | Oryza meridionalis | 42.74 | 1e-76 | 247 |
| LLPS-Ors-1105 | Oryza sativa | 42.69 | 4e-76 | 245 |
| LLPS-Xet-1105 | Xenopus tropicalis | 42.69 | 5e-73 | 236 |
| LLPS-Org-0807 | Oryza glaberrima | 42.69 | 4e-76 | 245 |
| LLPS-Orr-2345 | Oryza rufipogon | 42.69 | 4e-76 | 245 |
| LLPS-Orni-0900 | Oryza nivara | 42.69 | 4e-76 | 245 |
| LLPS-Ori-2105 | Oryza indica | 42.69 | 4e-76 | 245 |
| LLPS-Orb-1473 | Oryza barthii | 42.69 | 4e-76 | 245 |
| LLPS-Orgl-2022 | Oryza glumaepatula | 42.69 | 7e-76 | 244 |
| LLPS-Phv-2501 | Phaseolus vulgaris | 42.65 | 2e-74 | 241 |
| LLPS-Orbr-1778 | Oryza brachyantha | 42.49 | 1e-76 | 246 |
| LLPS-Mae-1169 | Manihot esculenta | 42.45 | 4e-76 | 245 |
| LLPS-Hov-2065 | Hordeum vulgare | 42.41 | 2e-74 | 243 |
| LLPS-Brd-1007 | Brachypodium distachyon | 42.29 | 6e-76 | 245 |
| LLPS-Viv-0748 | Vitis vinifera | 42.2 | 2e-75 | 243 |
| LLPS-Ten-2250 | Tetraodon nigroviridis | 41.5 | 6e-55 | 189 |
| LLPS-Tru-1195 | Triticum urartu | 40.89 | 4e-68 | 225 |
| LLPS-Gaga-3173 | Gallus gallus | 40.18 | 2e-62 | 209 |
| LLPS-Gas-0745 | Galdieria sulphuraria | 39.65 | 1e-56 | 201 |
| LLPS-Dac-0723 | Daucus carota | 39.57 | 2e-34 | 132 |
| LLPS-Vir-1467 | Vigna radiata | 39.24 | 1e-67 | 225 |
| LLPS-Cym-0841 | Cyanidioschyzon merolae | 37.63 | 5e-58 | 206 |
| LLPS-Orc-4050 | Oryctolagus cuniculus | 31.91 | 7e-26 | 110 |