LLPS-Sac-0630
SIR2
▼ OVERVIEW
| Status: | Reviewed |
| Protein Name: | NAD-dependent histone deacetylase SIR2; Regulatory protein SIR2; Silent information regulator 2 |
| Gene Name: | SIR2, MAR1, YDL042C, D2714 |
| Ensembl Gene: | YDL042C |
| Ensembl Protein: | YDL042C_mRNA |
| Organism: | Saccharomyces cerevisiae |
| Taxa ID: | 559292 |
| LLPS Type: | LLPS regulator |
▼ Classification
Condensates:
| Condensate | Description | Tissue/Cell | PMIDs |
|---|---|---|---|
| Stress granule | "...We identified 125 mutants that gave reproducible stress granules and/or P body phenotypes." | Saccharomyces cerevisiae | 23791177 |
| Others | "...Hos2 SPGs also contained Hst2, a Sir2 homologue, and several stress-related proteins, including Set3, Yca1, Hsp26, Hsp44, and some known components of stress granules." | Saccharomyces cerevisiae | 22337769 |
▼ FUNCTION
| NAD-dependent deacetylase, which participates in a wide range of cellular events including chromosome silencing, chromosome segregation, DNA recombination and the determination of life span. Involved in transcriptional repression of the silent mating-type loci HML and HMR and telomeric silencing via its association with SIR3 and SIR4. Plays a central role in ribosomal DNA (rDNA) silencing via its association with the RENT complex, preventing hyperrecombination, and repressing transcription from foreign promoters, which contributes to extending life span. Probably represses transcription via the formation of heterochromatin structure, which involves the compaction of chromatin fiber into a more condensed form, although this complex in at least one case can still bind euchromatic levels of positive transcription regulators. Although it displays some NAD-dependent histone deacetylase activity on histone H3K9Ac and H3K14Ac and histone H4K16Ac in vitro, such activity is unclear in vivo and may not be essential. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | YDL042C_mRNA | YDL042C_mRNA |
| UniProt | P06700, SIR2_YEAST, D6VRV4 | |
| GeneBank | BK006938, Z71781, Z74090, X01419 | DAA11814.1, CAA96447.1, CAA98600.1, CAA25667.1 |
| RefSeq | NM_001180101.1 | NP_010242.1 |
| Entrez | 851520 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MTIPHMKYAV SKTSENKVSN TVSPTQDKDA IRKQPDDIIN NDEPSHKKIK VAQPDSLRET 60 61 NTTDPLGHTK AALGEVASME LKPTNDMDPL AVSAASVVSM SNDVLKPETP KGPIIISKNP 120 121 SNGIFYGPSF TKRESLNARM FLKYYGAHKF LDTYLPEDLN SLYIYYLIKL LGFEVKDQAL 180 181 IGTINSIVHI NSQERVQDLG SAISVTNVED PLAKKQTVRL IKDLQRAINK VLCTRLRLSN 240 241 FFTIDHFIQK LHTARKILVL TGAGVSTSLG IPDFRSSEGF YSKIKHLGLD DPQDVFNYNI 300 301 FMHDPSVFYN IANMVLPPEK IYSPLHSFIK MLQMKGKLLR NYTQNIDNLE SYAGISTDKL 360 361 VQCHGSFATA TCVTCHWNLP GERIFNKIRN LELPLCPYCY KKRREYFPEG YNNKVGVAAS 420 421 QGSMSERPPY ILNSYGVLKP DITFFGEALP NKFHKSIRED ILECDLLICI GTSLKVAPVS 480 481 EIVNMVPSHV PQVLINRDPV KHAEFDLSLL GYCDDIAAMV AQKCGWTIPH KKWNDLKNKN 540 541 FKCQEKDKGV YVVTSDEHPK TL 562 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGACCATCC CACATATGAA ATACGCCGTA TCAAAGACTA GCGAAAATAA GGTTTCAAAT 60 61 ACAGTAAGCC CCACACAAGA TAAAGACGCG ATCAGAAAAC AACCCGATGA CATTATAAAT 120 121 AATGATGAAC CTTCACATAA GAAGATAAAA GTAGCACAGC CGGATTCCTT GAGGGAAACC 180 181 AACACAACAG ATCCACTTGG GCACACTAAA GCTGCGCTCG GAGAAGTGGC ATCGATGGAG 240 241 CTCAAACCAA CTAATGACAT GGATCCCTTG GCAGTGTCAG CAGCTTCAGT AGTGTCAATG 300 301 TCCAATGACG TTTTGAAACC AGAGACGCCC AAGGGGCCAA TCATAATCAG TAAAAACCCA 360 361 TCAAATGGTA TTTTCTATGG TCCCTCCTTC ACTAAACGAG AGTCTCTCAA TGCTCGAATG 420 421 TTTCTGAAAT ACTATGGTGC ACACAAATTT TTAGACACTT ACCTCCCCGA GGATTTGAAC 480 481 TCGTTATACA TTTACTATCT TATCAAGTTG CTAGGCTTTG AAGTTAAAGA TCAAGCGCTT 540 541 ATCGGCACCA TCAACAGTAT TGTCCATATC AACTCGCAAG AGCGTGTTCA AGATTTGGGA 600 601 AGTGCAATAT CTGTCACAAA TGTTGAAGAC CCATTGGCAA AAAAGCAAAC AGTTCGTCTA 660 661 ATCAAAGATT TGCAAAGAGC AATTAACAAA GTTCTATGTA CAAGATTAAG ATTATCCAAT 720 721 TTTTTCACTA TTGATCATTT TATTCAAAAA TTACATACCG CTAGAAAAAT TTTGGTCCTG 780 781 ACTGGTGCAG GTGTTTCAAC TTCATTAGGG ATCCCGGACT TCAGATCTTC TGAGGGGTTC 840 841 TATTCAAAGA TCAAACATTT GGGGCTCGAT GATCCCCAAG ACGTTTTCAA TTACAATATA 900 901 TTTATGCACG ACCCCTCTGT TTTCTATAAT ATTGCCAATA TGGTTTTACC TCCAGAAAAA 960 961 ATTTATTCTC CATTGCATAG TTTCATTAAG ATGCTACAAA TGAAAGGGAA ATTATTGAGA 1020 1021 AATTATACTC AAAACATTGA TAATTTGGAA TCTTATGCGG GAATAAGCAC AGATAAACTG 1080 1081 GTGCAGTGCC ATGGCTCTTT TGCTACTGCC ACCTGCGTTA CCTGCCATTG GAACCTACCC 1140 1141 GGTGAGAGGA TATTTAATAA AATTAGAAAC CTCGAACTTC CACTATGCCC GTACTGTTAC 1200 1201 AAAAAAAGAA GAGAATATTT CCCAGAGGGA TATAATAATA AAGTAGGTGT TGCTGCATCA 1260 1261 CAGGGTTCAA TGTCGGAAAG GCCTCCATAT ATCCTTAACT CATATGGCGT TCTCAAACCA 1320 1321 GATATCACAT TCTTTGGCGA AGCACTGCCA AATAAATTTC ATAAGAGCAT TCGCGAAGAT 1380 1381 ATCTTAGAAT GTGATTTGTT GATTTGCATT GGGACAAGTT TAAAAGTAGC GCCAGTGTCT 1440 1441 GAAATCGTAA ACATGGTTCC TTCCCACGTT CCCCAAGTCC TGATTAATCG TGATCCCGTC 1500 1501 AAGCACGCAG AATTTGATTT ATCTCTTTTG GGGTACTGTG ATGACATTGC AGCTATGGTA 1560 1561 GCCCAAAAAT GTGGCTGGAC GATTCCGCAT AAGAAATGGA ACGATTTGAA GAACAAGAAC 1620 1621 TTTAAATGCC AAGAGAAGGA TAAGGGCGTG TATGTCGTTA CATCAGATGA ACATCCCAAA 1680 1681 ACCCTCTAA 1689 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Chromatin silencing complex | |
Cellular Component | Nuclear chromatin | |
Cellular Component | Nuclear chromosome, telomeric region | |
Cellular Component | Nuclear heterochromatin | |
Cellular Component | Nuclear pericentric heterochromatin | |
Cellular Component | Nuclear telomeric heterochromatin | |
Cellular Component | Nucleolus | |
Cellular Component | RENT complex | |
Cellular Component | Set3 complex | |
Molecular Function | Histone deacetylase activity (H4-K16 specific) | |
Molecular Function | Metal ion binding | |
Molecular Function | NAD+ binding | |
Molecular Function | NAD-dependent histone deacetylase activity | |
Molecular Function | NAD-dependent histone deacetylase activity (H3-K14 specific) | |
Molecular Function | NAD-dependent histone deacetylase activity (H3-K9 specific) | |
Molecular Function | NAD-dependent histone deacetylase activity (H4-K16 specific) | |
Molecular Function | NAD-independent histone deacetylase activity | |
Biological Process | Chromatin assembly or disassembly | |
Biological Process | Chromatin organization | |
Biological Process | Chromatin silencing at rDNA | |
Biological Process | Chromatin silencing at silent mating-type cassette | |
Biological Process | Chromatin silencing at telomere | |
Biological Process | Chronological cell aging | |
Biological Process | Double-strand break repair via nonhomologous end joining | |
Biological Process | Establishment of protein-containing complex localization to telomere | |
Biological Process | Negative regulation of DNA amplification | |
Biological Process | Negative regulation of DNA recombination | |
Biological Process | Negative regulation of DNA replication | |
Biological Process | Replicative cell aging | |
Biological Process | Sister chromatid cohesion | |
Biological Process | Telomere tethering at nuclear periphery |
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Asg-0074 | Ashbya gossypii | 61.85 | 0.0 | 602 |
| LLPS-Kop-0694 | Komagataella pastoris | 50.47 | 1e-137 | 417 |
| LLPS-Ved-0099 | Verticillium dahliae | 49.72 | 7e-54 | 193 |
| LLPS-Asf-0151 | Aspergillus flavus | 48.9 | 4e-96 | 308 |
| LLPS-Cog-0869 | Colletotrichum gloeosporioides | 48.9 | 2e-99 | 318 |
| LLPS-Aso-1150 | Aspergillus oryzae | 48.59 | 3e-95 | 305 |
| LLPS-Coo-0210 | Colletotrichum orbiculare | 48.43 | 2e-96 | 312 |
| LLPS-Asc-0090 | Aspergillus clavatus | 48.11 | 3e-96 | 309 |
| LLPS-Blg-1046 | Blumeria graminis | 47.99 | 7e-96 | 307 |
| LLPS-Nef-1323 | Neosartorya fischeri | 47.17 | 5e-92 | 298 |
| LLPS-Cogr-0980 | Colletotrichum graminicola | 47.17 | 9e-98 | 314 |
| LLPS-Asni-0150 | Aspergillus niger | 47.02 | 1e-92 | 299 |
| LLPS-Asfu-0962 | Aspergillus fumigatus | 46.86 | 4e-91 | 295 |
| LLPS-Scj-0954 | Schizosaccharomyces japonicus | 46.67 | 2e-83 | 275 |
| LLPS-Fuv-0025 | Fusarium verticillioides | 46.56 | 3e-93 | 300 |
| LLPS-Asn-0828 | Aspergillus nidulans | 46.39 | 9e-94 | 302 |
| LLPS-Phn-0500 | Phaeosphaeria nodorum | 46.13 | 2e-84 | 278 |
| LLPS-Ast-1064 | Aspergillus terreus | 45.95 | 9e-88 | 299 |
| LLPS-Trv-0371 | Trichoderma virens | 45.94 | 3e-92 | 300 |
| LLPS-Fus-0683 | Fusarium solani | 45.57 | 2e-92 | 298 |
| LLPS-Nec-1249 | Neurospora crassa | 45.35 | 1e-92 | 304 |
| LLPS-Fuo-1407 | Fusarium oxysporum | 45.29 | 1e-92 | 299 |
| LLPS-Pyt-0496 | Pyrenophora teres | 45.22 | 1e-89 | 291 |
| LLPS-Gag-1161 | Gaeumannomyces graminis | 45.15 | 3e-83 | 277 |
| LLPS-Dar-3439 | Danio rerio | 45.04 | 1e-66 | 236 |
| LLPS-Beb-0565 | Beauveria bassiana | 44.98 | 5e-93 | 301 |
| LLPS-Pytr-1138 | Pyrenophora triticirepentis | 44.89 | 6e-91 | 295 |
| LLPS-Trr-1159 | Trichoderma reesei | 44.48 | 4e-90 | 295 |
| LLPS-Cii-0935 | Ciona intestinalis | 44.37 | 2e-69 | 243 |
| LLPS-Scm-2803 | Scophthalmus maximus | 44.37 | 2e-66 | 235 |
| LLPS-Dos-1254 | Dothistroma septosporum | 44.03 | 1e-84 | 280 |
| LLPS-Drm-1656 | Drosophila melanogaster | 43.3 | 1e-68 | 243 |
| LLPS-Yal-0904 | Yarrowia lipolytica | 42.57 | 6e-73 | 242 |
| LLPS-Pof-1120 | Poecilia formosa | 42.43 | 2e-69 | 243 |
| LLPS-Asm-2362 | Astyanax mexicanus | 42.32 | 9e-63 | 226 |
| LLPS-Miv-0765 | Microbotryum violaceum | 42.32 | 1e-71 | 246 |
| LLPS-Xim-0149 | Xiphophorus maculatus | 42.11 | 4e-69 | 242 |
| LLPS-Urm-1692 | Ursus maritimus | 42.0 | 3e-41 | 159 |
| LLPS-Orl-3170 | Oryzias latipes | 41.97 | 6e-68 | 238 |
| LLPS-Scp-0068 | Schizosaccharomyces pombe | 41.93 | 6e-82 | 271 |
| LLPS-Scc-0647 | Schizosaccharomyces cryophilus | 41.69 | 1e-82 | 273 |
| LLPS-Bot-1768 | Bos taurus | 41.64 | 2e-62 | 224 |
| LLPS-Tar-3726 | Takifugu rubripes | 41.64 | 8e-62 | 222 |
| LLPS-Ova-4301 | Ovis aries | 41.64 | 4e-63 | 224 |
| LLPS-Meg-0424 | Meleagris gallopavo | 41.64 | 6e-66 | 231 |
| LLPS-Pes-1061 | Pelodiscus sinensis | 41.64 | 3e-64 | 227 |
| LLPS-Spr-0265 | Sporisorium reilianum | 41.61 | 2e-75 | 256 |
| LLPS-Icp-2984 | Ictalurus punctatus | 41.39 | 2e-62 | 224 |
| LLPS-Mao-0794 | Magnaporthe oryzae | 41.34 | 3e-83 | 277 |
| LLPS-Leo-3220 | Lepisosteus oculatus | 41.31 | 5e-65 | 231 |
| LLPS-Anp-0792 | Anas platyrhynchos | 41.31 | 8e-66 | 231 |
| LLPS-Orn-0101 | Oreochromis niloticus | 41.31 | 4e-65 | 231 |
| LLPS-Ten-0311 | Tetraodon nigroviridis | 41.31 | 2e-63 | 223 |
| LLPS-Tag-3327 | Taeniopygia guttata | 41.31 | 9e-66 | 230 |
| LLPS-Xet-0889 | Xenopus tropicalis | 41.31 | 7e-64 | 228 |
| LLPS-Fud-1676 | Fukomys damarensis | 41.3 | 4e-63 | 224 |
| LLPS-Aim-2169 | Ailuropoda melanoleuca | 41.3 | 9e-63 | 223 |
| LLPS-Mup-4131 | Mustela putorius furo | 41.3 | 3e-63 | 223 |
| LLPS-Fec-3373 | Felis catus | 41.3 | 6e-62 | 223 |
| LLPS-Cap-2604 | Cavia porcellus | 41.3 | 2e-62 | 224 |
| LLPS-Caf-1499 | Canis familiaris | 41.3 | 6e-62 | 223 |
| LLPS-Crn-0785 | Cryptococcus neoformans | 41.23 | 4e-70 | 243 |
| LLPS-Abg-1116 | Absidia glauca | 41.19 | 3e-70 | 243 |
| LLPS-Sah-1569 | Sarcophilus harrisii | 41.16 | 7e-62 | 220 |
| LLPS-Fia-0351 | Ficedula albicollis | 40.98 | 8e-65 | 229 |
| LLPS-Gaga-2135 | Gallus gallus | 40.98 | 2e-64 | 231 |
| LLPS-Cea-2499 | Cercocebus atys | 40.96 | 1e-61 | 222 |
| LLPS-Sus-4403 | Sus scrofa | 40.96 | 9e-62 | 222 |
| LLPS-Chs-4589 | Chlorocebus sabaeus | 40.96 | 1e-61 | 222 |
| LLPS-Mea-1506 | Mesocricetus auratus | 40.96 | 6e-62 | 222 |
| LLPS-Mum-2544 | Mus musculus | 40.96 | 1e-61 | 222 |
| LLPS-Gog-4413 | Gorilla gorilla | 40.96 | 8e-62 | 223 |
| LLPS-Dio-0970 | Dipodomys ordii | 40.96 | 2e-62 | 223 |
| LLPS-Myl-3057 | Myotis lucifugus | 40.96 | 1e-62 | 223 |
| LLPS-Mal-3143 | Mandrillus leucophaeus | 40.96 | 4e-62 | 222 |
| LLPS-Paa-2440 | Papio anubis | 40.96 | 1e-61 | 222 |
| LLPS-Ran-1342 | Rattus norvegicus | 40.96 | 5e-61 | 220 |
| LLPS-Mod-2828 | Monodelphis domestica | 40.96 | 9e-63 | 224 |
| LLPS-Pat-3247 | Pan troglodytes | 40.96 | 1e-61 | 222 |
| LLPS-Rhb-3047 | Rhinopithecus bieti | 40.96 | 9e-62 | 222 |
| LLPS-Otg-0884 | Otolemur garnettii | 40.96 | 1e-61 | 222 |
| LLPS-Eqc-4554 | Equus caballus | 40.96 | 5e-62 | 223 |
| LLPS-Poa-2669 | Pongo abelii | 40.96 | 1e-61 | 222 |
| LLPS-Ict-1565 | Ictidomys tridecemlineatus | 40.96 | 1e-62 | 222 |
| LLPS-Anc-2103 | Anolis carolinensis | 40.96 | 3e-62 | 222 |
| LLPS-Aon-1163 | Aotus nancymaae | 40.61 | 3e-61 | 221 |
| LLPS-Maf-1133 | Macaca fascicularis | 40.61 | 1e-61 | 222 |
| LLPS-Caj-4267 | Callithrix jacchus | 40.61 | 3e-61 | 221 |
| LLPS-Loa-0232 | Loxodonta africana | 40.61 | 9e-61 | 217 |
| LLPS-Man-1184 | Macaca nemestrina | 40.61 | 1e-61 | 222 |
| LLPS-Hos-3117 | Homo sapiens | 40.61 | 1e-61 | 222 |
| LLPS-Orc-2802 | Oryctolagus cuniculus | 40.61 | 9e-62 | 222 |
| LLPS-Zyt-0442 | Zymoseptoria tritici | 40.51 | 2e-71 | 239 |
| LLPS-Lem-0705 | Leptosphaeria maculans | 40.49 | 2e-87 | 287 |
| LLPS-Mam-4597 | Macaca mulatta | 39.83 | 2e-45 | 172 |
| LLPS-Cym-0764 | Cyanidioschyzon merolae | 39.48 | 1e-59 | 210 |
| LLPS-Nol-2802 | Nomascus leucogenys | 39.25 | 3e-55 | 204 |
| LLPS-Gas-0285 | Galdieria sulphuraria | 38.51 | 2e-59 | 211 |
| LLPS-Scf-2843 | Scleropages formosus | 38.36 | 3e-56 | 206 |
| LLPS-Cis-1170 | Ciona savignyi | 37.07 | 1e-49 | 181 |
| LLPS-Usm-1245 | Ustilago maydis | 37.05 | 3e-43 | 165 |
| LLPS-Lac-3103 | Latimeria chalumnae | 35.33 | 4e-42 | 165 |
| LLPS-Gaa-3186 | Gasterosteus aculeatus | 34.51 | 1e-45 | 170 |
| LLPS-Scs-0276 | Sclerotinia sclerotiorum | 34.16 | 4e-40 | 156 |
| LLPS-Cas-0352 | Carlito syrichta | 32.69 | 9e-41 | 157 |
| LLPS-Pug-0398 | Puccinia graminis | 32.58 | 3e-40 | 156 |
| LLPS-Osl-0677 | Ostreococcus lucimarinus | 32.44 | 2e-40 | 154 |
| LLPS-Mel-0939 | Melampsora laricipopulina | 32.38 | 3e-40 | 152 |
| LLPS-Pap-3213 | Pan paniscus | 30.99 | 2e-42 | 162 |