LLPS-Sac-0149
PRE4
▼ OVERVIEW
| Status: | Reviewed |
| Protein Name: | Proteasome subunit beta type-7; Macropain subunit PRE4; Multicatalytic endopeptidase complex subunit PRE4; Proteasome component PRE4; Proteinase YSCE subunit PRE4 |
| Gene Name: | PRE4, YFR050C |
| Ensembl Gene: | YFR050C |
| Ensembl Protein: | YFR050C_mRNA |
| Organism: | Saccharomyces cerevisiae |
| Taxa ID: | 559292 |
| LLPS Type: | LLPS regulator |
▼ Classification
Condensates:
| Condensate | Description | Tissue/Cell | PMIDs |
|---|---|---|---|
| P-body | "...When overexpressed, GFP-Rpm2p does not impact the number and size of P bodies, however, it prevents their disappearance when translation elongation is inhibited by cycloheximide. Proteasome mutants, ump1-2 and pre4-2, that bypass essential Rpm2p function, also stabilize P bodies. The stabilization of P bodies by Rpm2p may occur through reduced protein degradation since GFP-Rpm2p expressing cells have lower levels of ubiquitin." | Saccharomyces cerevisiae | 17267405 |
| Nucleolus, Others | Predicted from orthologs | N/A | (View) |
▼ FUNCTION
| The proteasome degrades poly-ubiquitinated proteins in the cytoplasm and in the nucleus. It is essential for the regulated turnover of proteins and for the removal of misfolded proteins. The proteasome is a multicatalytic proteinase complex that is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. It has an ATP-dependent proteolytic activity. PRE3 and PRE4 are necessary for the peptidyl-glutamyl-peptide-hydrolyzing activity. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | YFR050C_mRNA | YFR050C_mRNA |
| UniProt | P30657, PSB7_YEAST, D6VTT3, E9P8Z8 | |
| GeneBank | D50617, BK006940, X68663, AY692947 | BAA09289.1, DAA12493.1, CAA48629.1, AAT92966.1 |
| RefSeq | NM_001180015.1 | NP_116708.1 |
| Entrez | 850611 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MNHDPFSWGR PADSTYGAYN TQIANAGASP MVNTQQPIVT GTSVISMKYD NGVIIAADNL 60 61 GSYGSLLRFN GVERLIPVGD NTVVGISGDI SDMQHIERLL KDLVTENAYD NPLADAEEAL 120 121 EPSYIFEYLA TVMYQRRSKM NPLWNAIIVA GVQSNGDQFL RYVNLLGVTY SSPTLATGFG 180 181 AHMANPLLRK VVDRESDIPK TTVQVAEEAI VNAMRVLYYR DARSSRNFSL AIIDKNTGLT 240 241 FKKNLQVENM KWDFAKDIKG YGTQKI 266 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGAATCACG ATCCTTTCAG TTGGGGCAGG CCCGCAGACT CTACTTATGG TGCTTACAAT 60 61 ACACAAATTG CGAACGCAGG CGCCTCTCCC ATGGTTAATA CACAGCAACC AATAGTAACA 120 121 GGTACTTCCG TCATATCAAT GAAATATGAT AATGGGGTTA TCATTGCAGC AGATAATTTA 180 181 GGTTCATATG GCTCTCTTCT AAGATTCAAT GGCGTGGAGA GGCTTATTCC CGTGGGTGAT 240 241 AACACCGTTG TGGGCATTTC AGGTGATATT TCTGATATGC AACACATTGA GAGATTATTG 300 301 AAAGATCTAG TCACTGAAAA TGCGTATGAC AATCCTCTAG CGGATGCTGA AGAAGCGCTC 360 361 GAACCCAGCT ATATTTTTGA ATATCTAGCT ACCGTCATGT ACCAGCGAAG ATCAAAGATG 420 421 AATCCACTTT GGAATGCCAT CATTGTTGCT GGTGTACAGT CTAATGGTGA TCAATTCCTA 480 481 AGATACGTAA ATCTTCTAGG TGTTACGTAT TCGTCTCCCA CCTTGGCCAC AGGTTTTGGG 540 541 GCACACATGG CAAACCCACT GTTAAGGAAA GTTGTAGATC GAGAATCTGA TATTCCAAAG 600 601 ACCACTGTAC AGGTTGCTGA AGAGGCGATC GTAAATGCAA TGAGAGTTCT TTATTATAGA 660 661 GATGCCCGTT CTTCTAGAAA CTTCTCACTG GCCATAATTG ATAAGAACAC AGGTTTAACT 720 721 TTCAAGAAAA ACTTGCAAGT TGAAAACATG AAATGGGACT TCGCCAAGGA TATTAAAGGC 780 781 TACGGTACTC AAAAAATTTA G 801 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Cytoplasm | |
Cellular Component | Endoplasmic reticulum membrane | |
Cellular Component | Nucleus | |
Cellular Component | Proteasome core complex | |
Cellular Component | Proteasome core complex, beta-subunit complex | |
Cellular Component | Proteasome storage granule | |
Molecular Function | Endopeptidase activity | |
Molecular Function | Threonine-type endopeptidase activity | |
Biological Process | Proteasomal protein catabolic process | |
Biological Process | Proteasomal ubiquitin-independent protein catabolic process | |
Biological Process | Proteasome assembly | |
Biological Process | Proteasome-mediated ubiquitin-dependent protein catabolic process |
▼ ANNOTATION
| Disorder | ||||
| IUPred2A | ||||
| Physicochemical | ||||
| Compute pI/Mw | AAindex | |||
| Function | ||||
| PIRSF | ||||
| Structure | ||||
| PDB | ||||
| Localization | ||||
| NLSdb | ||||
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Asg-1197 | Ashbya gossypii | 67.29 | 5e-130 | 376 |
| LLPS-Kop-0590 | Komagataella pastoris | 57.25 | 1e-108 | 322 |
| LLPS-Scs-0752 | Sclerotinia sclerotiorum | 51.14 | 5e-89 | 271 |
| LLPS-Phn-0876 | Phaeosphaeria nodorum | 50.57 | 5e-86 | 266 |
| LLPS-Tum-1331 | Tuber melanosporum | 49.81 | 2e-81 | 253 |
| LLPS-Blg-0413 | Blumeria graminis | 49.62 | 8e-85 | 261 |
| LLPS-Yal-0666 | Yarrowia lipolytica | 49.06 | 7e-82 | 254 |
| LLPS-Dos-0716 | Dothistroma septosporum | 48.91 | 6e-89 | 272 |
| LLPS-Zyt-0055 | Zymoseptoria tritici | 48.74 | 3e-85 | 263 |
| LLPS-Fus-1074 | Fusarium solani | 48.5 | 4e-81 | 252 |
| LLPS-Pytr-1387 | Pyrenophora triticirepentis | 48.48 | 3e-83 | 257 |
| LLPS-Trv-0311 | Trichoderma virens | 48.12 | 5e-85 | 261 |
| LLPS-Fuo-0922 | Fusarium oxysporum | 48.12 | 2e-81 | 252 |
| LLPS-Pyt-0052 | Pyrenophora teres | 48.11 | 8e-83 | 256 |
| LLPS-Cogr-1520 | Colletotrichum graminicola | 47.74 | 1e-82 | 255 |
| LLPS-Fuv-1059 | Fusarium verticillioides | 47.74 | 2e-81 | 252 |
| LLPS-Scc-0321 | Schizosaccharomyces cryophilus | 47.71 | 6e-72 | 228 |
| LLPS-Beb-0676 | Beauveria bassiana | 47.37 | 1e-80 | 250 |
| LLPS-Cog-0604 | Colletotrichum gloeosporioides | 47.37 | 8e-81 | 251 |
| LLPS-Trr-1212 | Trichoderma reesei | 47.37 | 6e-83 | 256 |
| LLPS-Put-0442 | Puccinia triticina | 47.28 | 2e-67 | 217 |
| LLPS-Asn-0496 | Aspergillus nidulans | 46.99 | 4e-78 | 244 |
| LLPS-Aso-0205 | Aspergillus oryzae | 46.97 | 2e-77 | 242 |
| LLPS-Asf-0483 | Aspergillus flavus | 46.97 | 1e-76 | 240 |
| LLPS-Scp-0570 | Schizosaccharomyces pombe | 46.95 | 4e-72 | 229 |
| LLPS-Scj-0530 | Schizosaccharomyces japonicus | 46.82 | 1e-67 | 217 |
| LLPS-Usm-0122 | Ustilago maydis | 46.78 | 2e-64 | 209 |
| LLPS-Asni-0147 | Aspergillus niger | 46.59 | 2e-77 | 242 |
| LLPS-Nec-0539 | Neurospora crassa | 46.49 | 3e-82 | 254 |
| LLPS-Mel-0424 | Melampsora laricipopulina | 46.4 | 1e-75 | 238 |
| LLPS-Coo-0168 | Colletotrichum orbiculare | 46.24 | 5e-82 | 254 |
| LLPS-Asfu-1024 | Aspergillus fumigatus | 46.21 | 2e-78 | 245 |
| LLPS-Asc-0437 | Aspergillus clavatus | 46.21 | 2e-79 | 247 |
| LLPS-Spr-0527 | Sporisorium reilianum | 46.19 | 1e-64 | 209 |
| LLPS-Orl-1808 | Oryzias latipes | 46.12 | 3e-61 | 200 |
| LLPS-Ast-1157 | Aspergillus terreus | 45.86 | 6e-77 | 241 |
| LLPS-Nef-0845 | Neosartorya fischeri | 45.83 | 2e-78 | 244 |
| LLPS-Lem-1475 | Leptosphaeria maculans | 45.74 | 1e-80 | 251 |
| LLPS-Abg-0592 | Absidia glauca | 45.59 | 5e-66 | 213 |
| LLPS-Ved-0629 | Verticillium dahliae | 45.32 | 1e-81 | 253 |
| LLPS-Crn-0027 | Cryptococcus neoformans | 45.16 | 3e-72 | 229 |
| LLPS-Pes-0838 | Pelodiscus sinensis | 44.89 | 1e-59 | 195 |
| LLPS-Pof-0519 | Poecilia formosa | 44.87 | 3e-61 | 200 |
| LLPS-Gaa-0816 | Gasterosteus aculeatus | 44.74 | 3e-59 | 196 |
| LLPS-Xim-2686 | Xiphophorus maculatus | 44.44 | 2e-60 | 199 |
| LLPS-Mea-3004 | Mesocricetus auratus | 44.4 | 2e-62 | 204 |
| LLPS-Mod-0357 | Monodelphis domestica | 44.23 | 2e-56 | 187 |
| LLPS-Pug-1049 | Puccinia graminis | 44.09 | 8e-72 | 228 |
| LLPS-Cas-2343 | Carlito syrichta | 44.02 | 3e-62 | 203 |
| LLPS-Scm-2795 | Scophthalmus maximus | 44.02 | 4e-60 | 199 |
| LLPS-Myl-0083 | Myotis lucifugus | 44.02 | 3e-62 | 203 |
| LLPS-Cap-1014 | Cavia porcellus | 43.97 | 4e-62 | 202 |
| LLPS-Mum-3757 | Mus musculus | 43.97 | 8e-62 | 202 |
| LLPS-Ran-1484 | Rattus norvegicus | 43.88 | 1e-62 | 203 |
| LLPS-Glm-0742 | Glycine max | 43.81 | 1e-44 | 156 |
| LLPS-Eqc-0629 | Equus caballus | 43.59 | 9e-62 | 203 |
| LLPS-Orc-0025 | Oryctolagus cuniculus | 43.59 | 2e-61 | 201 |
| LLPS-Orn-0147 | Oreochromis niloticus | 43.59 | 4e-61 | 200 |
| LLPS-Bot-0552 | Bos taurus | 43.59 | 8e-62 | 202 |
| LLPS-Sus-0338 | Sus scrofa | 43.59 | 2e-61 | 201 |
| LLPS-Mao-0042 | Magnaporthe oryzae | 43.54 | 3e-79 | 247 |
| LLPS-Otg-2935 | Otolemur garnettii | 43.53 | 4e-61 | 200 |
| LLPS-Caj-2414 | Callithrix jacchus | 43.53 | 6e-61 | 200 |
| LLPS-Pot-1022 | Populus trichocarpa | 43.5 | 4e-47 | 163 |
| LLPS-Amt-0025 | Amborella trichopoda | 43.5 | 2e-45 | 159 |
| LLPS-Lac-2397 | Latimeria chalumnae | 43.4 | 1e-61 | 202 |
| LLPS-Scf-1611 | Scleropages formosus | 43.4 | 7e-60 | 198 |
| LLPS-Dio-1943 | Dipodomys ordii | 43.23 | 4e-59 | 194 |
| LLPS-Ten-0582 | Tetraodon nigroviridis | 43.16 | 1e-59 | 196 |
| LLPS-Ict-2515 | Ictidomys tridecemlineatus | 43.16 | 1e-60 | 199 |
| LLPS-Tar-0274 | Takifugu rubripes | 43.16 | 2e-59 | 196 |
| LLPS-Sah-2748 | Sarcophilus harrisii | 43.16 | 6e-60 | 197 |
| LLPS-Asm-0182 | Astyanax mexicanus | 43.15 | 4e-60 | 197 |
| LLPS-Tag-1588 | Taeniopygia guttata | 43.14 | 7e-63 | 205 |
| LLPS-Mam-1167 | Macaca mulatta | 43.1 | 7e-60 | 197 |
| LLPS-Poa-0357 | Pongo abelii | 43.1 | 3e-60 | 198 |
| LLPS-Nol-2641 | Nomascus leucogenys | 43.1 | 4e-60 | 198 |
| LLPS-Man-0218 | Macaca nemestrina | 43.1 | 7e-60 | 197 |
| LLPS-Hos-2928 | Homo sapiens | 43.1 | 3e-60 | 198 |
| LLPS-Pat-0997 | Pan troglodytes | 43.1 | 3e-60 | 198 |
| LLPS-Pap-1268 | Pan paniscus | 43.1 | 3e-60 | 198 |
| LLPS-Rhb-1251 | Rhinopithecus bieti | 43.1 | 7e-60 | 197 |
| LLPS-Mal-1983 | Mandrillus leucophaeus | 43.1 | 6e-60 | 197 |
| LLPS-Paa-2176 | Papio anubis | 43.1 | 7e-60 | 197 |
| LLPS-Cea-0029 | Cercocebus atys | 43.1 | 7e-60 | 197 |
| LLPS-Maf-3155 | Macaca fascicularis | 43.1 | 7e-60 | 197 |
| LLPS-Chs-3326 | Chlorocebus sabaeus | 43.1 | 4e-60 | 198 |
| LLPS-Gog-2961 | Gorilla gorilla | 43.1 | 3e-60 | 198 |
| LLPS-Gas-0004 | Galdieria sulphuraria | 42.92 | 2e-48 | 166 |
| LLPS-Ora-0290 | Ornithorhynchus anatinus | 42.86 | 2e-56 | 186 |
| LLPS-Fud-0043 | Fukomys damarensis | 42.86 | 2e-59 | 196 |
| LLPS-Icp-2717 | Ictalurus punctatus | 42.86 | 6e-55 | 182 |
| LLPS-Phv-1103 | Phaseolus vulgaris | 42.79 | 9e-45 | 157 |
| LLPS-Ova-1714 | Ovis aries | 42.63 | 1e-62 | 204 |
| LLPS-Anp-1265 | Anas platyrhynchos | 42.47 | 6e-31 | 118 |
| LLPS-Caf-0066 | Canis familiaris | 42.23 | 2e-61 | 201 |
| LLPS-Fec-1657 | Felis catus | 42.23 | 3e-62 | 203 |
| LLPS-Mup-0807 | Mustela putorius furo | 42.23 | 1e-61 | 202 |
| LLPS-Via-0341 | Vigna angularis | 42.05 | 2e-44 | 155 |
| LLPS-Loa-0736 | Loxodonta africana | 42.02 | 2e-62 | 203 |
| LLPS-Thc-1365 | Theobroma cacao | 41.9 | 2e-45 | 159 |
| LLPS-Sob-0029 | Sorghum bicolor | 41.87 | 1e-41 | 149 |
| LLPS-Tra-0054 | Triticum aestivum | 41.87 | 6e-41 | 147 |
| LLPS-Meg-1749 | Meleagris gallopavo | 41.85 | 5e-57 | 189 |
| LLPS-Aim-1932 | Ailuropoda melanoleuca | 41.83 | 2e-60 | 198 |
| LLPS-Miv-1107 | Microbotryum violaceum | 41.67 | 3e-66 | 214 |
| LLPS-Urm-0488 | Ursus maritimus | 41.63 | 2e-60 | 199 |
| LLPS-Gag-1175 | Gaeumannomyces graminis | 41.46 | 5e-72 | 229 |
| LLPS-Dar-2015 | Danio rerio | 41.43 | 8e-60 | 197 |
| LLPS-Brd-0575 | Brachypodium distachyon | 41.43 | 3e-41 | 148 |
| LLPS-Zem-1086 | Zea mays | 41.38 | 5e-41 | 148 |
| LLPS-Sei-1493 | Setaria italica | 41.38 | 4e-41 | 148 |
| LLPS-Nia-1490 | Nicotiana attenuata | 40.99 | 1e-45 | 159 |
| LLPS-Sot-0000 | Solanum tuberosum | 40.97 | 7e-47 | 163 |
| LLPS-Orbr-0785 | Oryza brachyantha | 40.89 | 3e-42 | 150 |
| LLPS-Tru-0946 | Triticum urartu | 40.85 | 1e-41 | 149 |
| LLPS-Lep-1150 | Leersia perrieri | 40.69 | 3e-42 | 151 |
| LLPS-Sol-0648 | Solanum lycopersicum | 40.64 | 4e-43 | 157 |
| LLPS-Mua-0205 | Musa acuminata | 40.54 | 6e-42 | 150 |
| LLPS-Anc-0298 | Anolis carolinensis | 40.53 | 2e-55 | 184 |
| LLPS-Gaga-3076 | Gallus gallus | 40.47 | 7e-60 | 197 |
| LLPS-Hea-0075 | Helianthus annuus | 40.25 | 4e-45 | 159 |
| LLPS-Chr-1221 | Chlamydomonas reinhardtii | 40.17 | 4e-50 | 172 |
| LLPS-Mae-0049 | Manihot esculenta | 40.09 | 5e-43 | 153 |
| LLPS-Aon-1301 | Aotus nancymaae | 40.09 | 6e-50 | 171 |
| LLPS-Dac-1594 | Daucus carota | 39.91 | 3e-44 | 155 |
| LLPS-Hov-1167 | Hordeum vulgare | 39.82 | 4e-41 | 149 |
| LLPS-Met-0217 | Medicago truncatula | 39.71 | 2e-44 | 155 |
| LLPS-Coc-0673 | Corchorus capsularis | 39.64 | 3e-45 | 158 |
| LLPS-Viv-1728 | Vitis vinifera | 39.64 | 1e-44 | 157 |
| LLPS-Php-0902 | Physcomitrella patens | 39.53 | 2e-42 | 152 |
| LLPS-Gor-0505 | Gossypium raimondii | 39.24 | 3e-46 | 161 |
| LLPS-Chc-0508 | Chondrus crispus | 39.09 | 2e-45 | 160 |
| LLPS-Ere-0545 | Erinaceus europaeus | 39.01 | 5e-27 | 108 |
| LLPS-Bro-1228 | Brassica oleracea | 38.74 | 5e-43 | 152 |
| LLPS-Brn-0498 | Brassica napus | 38.74 | 5e-43 | 152 |
| LLPS-Cus-0493 | Cucumis sativus | 38.37 | 2e-45 | 159 |
| LLPS-Prp-0638 | Prunus persica | 38.29 | 2e-44 | 156 |
| LLPS-Arl-0494 | Arabidopsis lyrata | 38.01 | 7e-42 | 150 |
| LLPS-Art-0013 | Arabidopsis thaliana | 37.67 | 2e-42 | 151 |
| LLPS-Brr-1162 | Brassica rapa | 37.56 | 7e-43 | 152 |
| LLPS-Orm-0298 | Oryza meridionalis | 37.39 | 5e-41 | 153 |
| LLPS-Sem-2171 | Selaginella moellendorffii | 37.3 | 2e-44 | 156 |
| LLPS-Orr-0729 | Oryza rufipogon | 37.23 | 2e-43 | 154 |
| LLPS-Org-1645 | Oryza glaberrima | 37.23 | 2e-43 | 154 |
| LLPS-Orp-0872 | Oryza punctata | 37.23 | 8e-43 | 152 |
| LLPS-Ors-0219 | Oryza sativa | 37.23 | 2e-43 | 154 |
| LLPS-Drm-1742 | Drosophila melanogaster | 37.16 | 7e-41 | 148 |
| LLPS-Orgl-0162 | Oryza glumaepatula | 37.07 | 1e-42 | 154 |
| LLPS-Orb-0701 | Oryza barthii | 37.07 | 1e-42 | 154 |
| LLPS-Ori-0116 | Oryza indica | 37.02 | 9e-44 | 155 |
| LLPS-Orni-1345 | Oryza nivara | 36.96 | 1e-43 | 154 |
| LLPS-Cii-0342 | Ciona intestinalis | 36.15 | 2e-50 | 172 |
| LLPS-Osl-0755 | Ostreococcus lucimarinus | 35.22 | 2e-42 | 152 |
| LLPS-Cym-0133 | Cyanidioschyzon merolae | 34.67 | 8e-37 | 136 |
| LLPS-Vir-0007 | Vigna radiata | 32.34 | 3e-23 | 99.0 |
| LLPS-Fia-0961 | Ficedula albicollis | 31.33 | 7e-28 | 112 |
| LLPS-Cae-0193 | Caenorhabditis elegans | 25.68 | 8e-25 | 103 |