DrLLPS - Data resource of LLPS

LLPS-Mum-4054
Rpa2

▼ OVERVIEW


Status:	Unreviewed
Protein Name:	Replication protein A 32 kDa subunit
Gene Name:	Rpa2, Rpa34
Ensembl Gene:	ENSMUSG00000028884.14
Ensembl Protein:	ENSMUSP00000099621.4
Organism:	Mus musculus
Taxa ID:	10090
LLPS Type:	Client

▼ PROPERTY

——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:

Condensate	Evidence	Orthologs
Stress granule, Others	Predicted from orthologs	(View)

Organism	DrLLPS ID	UniProt ID	LLPS type	Condensate	Identity	E-value	Score
Saccharomyces cerevisiae	LLPS-Sac-0225	P26754	Client	Stress granule, Others	27.55	3e-15	77.0

▼ FUNCTION

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

▼ CROSS REFERENCE

Database	Nucleotide ID	Protein ID
Ensembl	ENSMUST00000102561.10	ENSMUSP00000099621.4
Ensembl	ENSMUST00000156968.1	ENSMUSP00000123780.1
UniProt		Q62193, RFA2_MOUSE
GeneBank	D00812	BAA00693.1
Entrez	19891

▼ SEQUENCE

Protein Sequence (FASTA)
1 MWNSGFESFS SSTYGGAGGY TQSPGGFGSP TPSQAEKKSR VRAQHIVPCT ISQLLSATLT 60 61 DEVFRIGDVE ISQVTIVGII RHAEKAPTNI VYKIDDMTAP PMDVRQWVDT DDASGENAVV 120 121 PPETYVKVAG HLRSFQNKKS LVAFKIIPLE DMNEFTAHIL EVVNSHMMLS KPNSQASAGR 180 181 PSMSNPGMSE PGNFSGNNFM PANGLTVVQN QVLNLIKACP RPEGLNFQDL RSQLQHMPVP 240 241 SIKQAVDFLC NEGHIYSTVD DDHFKSTDAE 270
Nucleotide CDS Sequence (FASTA)
1 ATGTGGAATA GCGGATTCGA AAGCTTCAGC AGCTCCACCT ATGGCGGAGC AGGCGGCTAC 60 61 ACGCAGTCCC CCGGTGGCTT CGGGTCGCCC ACACCGTCGC AGGCGGAGAA GAAATCGAGA 120 121 GTCCGAGCCC AGCATATTGT GCCCTGTACC ATATCCCAGC TGCTTTCTGC TACTCTGACT 180 181 GATGAAGTGT TCAGAATTGG AGATGTCGAG ATTTCACAGG TCACTATTGT GGGGATAATC 240 241 AGACATGCGG AGAAGGCTCC AACCAACATT GTTTACAAGA TAGACGATAT GACAGCTCCG 300 301 CCCATGGATG TTCGTCAGTG GGTTGACACG GATGATGCCA GTGGTGAGAA CGCCGTGGTT 360 361 CCTCCCGAAA CATACGTGAA AGTCGCTGGT CACCTCAGGT CTTTCCAGAA CAAAAAGAGC 420 421 TTGGTGGCCT TTAAGATCAT TCCTCTGGAA GACATGAATG AGTTCACCGC ACACATCCTG 480 481 GAAGTGGTCA ATTCACACAT GATGCTCAGC AAGCCCAACA GCCAGGCCTC TGCAGGGAGA 540 541 CCATCGATGA GCAACCCAGG AATGAGTGAG CCGGGGAACT TCAGCGGGAA TAACTTCATG 600 601 CCAGCAAATG GCCTCACTGT GGTCCAAAAC CAGGTGCTGA ATTTGATCAA GGCTTGCCCA 660 661 AGACCTGAAG GCCTGAACTT CCAGGACCTC AGGAGCCAGC TCCAGCATAT GCCTGTTCCC 720 721 TCAATCAAGC AAGCAGTGGA TTTTCTGTGC AACGAGGGCC ACATTTACTC TACTGTGGAC 780 781 GACGATCACT TTAAGTCTAC AGATGCAGAG TGA 813

▼ KEYWORD

ID	Family
KW-0007	Acetylation
KW-0181	Complete proteome
KW-0227	DNA damage
KW-0233	DNA recombination
KW-0234	DNA repair
KW-0235	DNA replication
KW-0238	DNA-binding
KW-1017	Isopeptide bond
KW-0539	Nucleus
KW-0597	Phosphoprotein
KW-1185	Reference proteome
KW-0832	Ubl conjugation

▼ GENE ONTOLOGY

ID	Classification	Description
GO:0000785	Cellular Component	Chromatin
GO:0000781	Cellular Component	Chromosome, telomeric region
GO:0000794	Cellular Component	Condensed nuclear chromosome
GO:0005662	Cellular Component	DNA replication factor A complex
GO:0016604	Cellular Component	Nuclear body
GO:0005654	Cellular Component	Nucleoplasm
GO:0005634	Cellular Component	Nucleus
GO:0016605	Cellular Component	PML body
GO:0035861	Cellular Component	Site of double-strand break
GO:0003684	Molecular Function	Damaged DNA binding
GO:0003677	Molecular Function	DNA binding
GO:0003690	Molecular Function	Double-stranded DNA binding
GO:0019899	Molecular Function	Enzyme binding
GO:0098505	Molecular Function	G-rich strand telomeric DNA binding
GO:0047485	Molecular Function	Protein N-terminus binding
GO:0019903	Molecular Function	Protein phosphatase binding
GO:0043565	Molecular Function	Sequence-specific DNA binding
GO:0003697	Molecular Function	Single-stranded DNA binding
GO:0031625	Molecular Function	Ubiquitin protein ligase binding
GO:0006284	Biological Process	Base-excision repair
GO:0006260	Biological Process	DNA replication
GO:0006265	Biological Process	DNA topological change
GO:0006268	Biological Process	DNA unwinding involved in DNA replication
GO:0000724	Biological Process	Double-strand break repair via homologous recombination
GO:0030491	Biological Process	Heteroduplex formation
GO:0006298	Biological Process	Mismatch repair
GO:0031571	Biological Process	Mitotic G1 DNA damage checkpoint
GO:0006312	Biological Process	Mitotic recombination
GO:0006289	Biological Process	Nucleotide-excision repair
GO:0051096	Biological Process	Positive regulation of helicase activity
GO:0034502	Biological Process	Protein localization to chromosome
GO:0007131	Biological Process	Reciprocal meiotic recombination
GO:2000001	Biological Process	Regulation of DNA damage checkpoint
GO:0010569	Biological Process	Regulation of double-strand break repair via homologous recombination
GO:0000723	Biological Process	Telomere maintenance
GO:0000722	Biological Process	Telomere maintenance via recombination
GO:0007004	Biological Process	Telomere maintenance via telomerase

▼ ANNOTATION

Disorder
D²P²	IUPred2A

Domain
SMART	Pfam	InterPro	LCR-eXXXplorer	CDD
Gene3D

PTM
EPSD	PhosphoSitePlus	dbPTM

Mutation
CGAP

Interaction
HINT	RISE	RAIN	Mentha	PINA
IID	iRefIndex	MIST	RNAct

Physicochemical
Compute pI/Mw	AAindex

Function
PIRSF	CORUM

Localization
COMPARTMENTS

Expression
ArrayExpress	GXD	TISSUES

Element
miRWalk	RAID	microRNA.org

▼ ORTHOLOGY

DrLLPS ID	Organism	Identity	E-value	Score
LLPS-Ran-2983	Rattus norvegicus	95.56	4e-179	501
LLPS-Mea-4205	Mesocricetus auratus	92.22	1e-176	495
LLPS-Ict-0829	Ictidomys tridecemlineatus	89.3	9e-172	483
LLPS-Nol-4153	Nomascus leucogenys	88.89	1e-169	477
LLPS-Cea-4870	Cercocebus atys	88.89	1e-169	477
LLPS-Gog-1608	Gorilla gorilla	88.89	1e-169	477
LLPS-Tut-1861	Tursiops truncatus	88.76	2e-161	459
LLPS-Orc-3205	Oryctolagus cuniculus	88.74	3e-151	429
LLPS-Caj-4661	Callithrix jacchus	88.72	1e-164	468
LLPS-Mal-0782	Mandrillus leucophaeus	88.72	1e-164	468
LLPS-Poa-2474	Pongo abelii	88.52	7e-169	478
LLPS-Mam-0294	Macaca mulatta	88.52	5e-169	476
LLPS-Chs-3583	Chlorocebus sabaeus	88.52	1e-169	477
LLPS-Maf-3077	Macaca fascicularis	88.52	5e-169	476
LLPS-Bot-2089	Bos taurus	88.52	2e-168	474
LLPS-Hos-4845	Homo sapiens	88.35	1e-163	466
LLPS-Paa-0594	Papio anubis	88.35	5e-164	466
LLPS-Pap-3499	Pan paniscus	88.15	7e-169	475
LLPS-Man-1161	Macaca nemestrina	88.15	1e-168	475
LLPS-Eqc-3838	Equus caballus	88.15	2e-168	474
LLPS-Sus-0997	Sus scrofa	88.15	1e-163	462
LLPS-Myl-0482	Myotis lucifugus	88.15	1e-167	472
LLPS-Ova-3984	Ovis aries	87.78	5e-168	473
LLPS-Cas-3002	Carlito syrichta	87.45	1e-149	425
LLPS-Fud-4280	Fukomys damarensis	87.04	8e-165	465
LLPS-Mup-3346	Mustela putorius furo	86.75	5e-158	448
LLPS-Loa-3263	Loxodonta africana	86.3	2e-164	464
LLPS-Caf-4214	Canis familiaris	85.87	2e-161	457
LLPS-Aim-2570	Ailuropoda melanoleuca	85.71	3e-146	417
LLPS-Dio-3835	Dipodomys ordii	85.31	8e-155	439
LLPS-Rhb-2936	Rhinopithecus bieti	85.13	9e-156	442
LLPS-Fec-3061	Felis catus	84.81	3e-163	461
LLPS-Cap-4431	Cavia porcellus	84.81	8e-161	455
LLPS-Aon-4028	Aotus nancymaae	84.67	5e-161	456
LLPS-Otg-2787	Otolemur garnettii	83.46	7e-156	442
LLPS-Mod-1503	Monodelphis domestica	78.55	2e-144	414
LLPS-Pat-3673	Pan troglodytes	78.47	3e-154	439
LLPS-Sah-3548	Sarcophilus harrisii	76.9	2e-145	416
LLPS-Tag-1590	Taeniopygia guttata	70.68	4e-125	363
LLPS-Meg-2829	Meleagris gallopavo	69.48	5e-124	361
LLPS-Gaga-1977	Gallus gallus	69.48	2e-124	362
LLPS-Fia-3778	Ficedula albicollis	69.26	2e-112	330
LLPS-Ora-3222	Ornithorhynchus anatinus	68.47	6e-95	286
LLPS-Xet-3563	Xenopus tropicalis	64.07	9e-101	301
LLPS-Anc-3561	Anolis carolinensis	63.86	3e-115	339
LLPS-Pes-2702	Pelodiscus sinensis	63.79	1e-73	229
LLPS-Leo-1231	Lepisosteus oculatus	59.93	2e-110	327
LLPS-Scf-4083	Scleropages formosus	58.03	5e-105	313
LLPS-Pof-3562	Poecilia formosa	57.5	5e-103	308
LLPS-Xim-3441	Xiphophorus maculatus	57.14	1e-102	307
LLPS-Orl-3630	Oryzias latipes	56.25	3e-101	304
LLPS-Scm-3222	Scophthalmus maximus	55.64	2e-101	304
LLPS-Gaa-1357	Gasterosteus aculeatus	55.64	3e-102	306
LLPS-Orn-3007	Oreochromis niloticus	55.59	2e-103	310
LLPS-Dar-1184	Danio rerio	54.51	5e-102	305
LLPS-Icp-4033	Ictalurus punctatus	54.21	3e-97	294
LLPS-Tar-2157	Takifugu rubripes	52.17	4e-101	303
LLPS-Lac-3190	Latimeria chalumnae	50.0	2e-77	242
LLPS-Asm-0364	Astyanax mexicanus	46.34	9e-71	224
LLPS-Ten-2209	Tetraodon nigroviridis	38.27	2e-44	157
LLPS-Coo-1237	Colletotrichum orbiculare	35.38	1e-16	81.3
LLPS-Scs-0698	Sclerotinia sclerotiorum	35.08	4e-27	111
LLPS-Kop-0381	Komagataella pastoris	34.57	8e-16	78.6
LLPS-Mao-0714	Magnaporthe oryzae	32.89	4e-23	100
LLPS-Cus-0123	Cucumis sativus	32.76	1e-18	86.3
LLPS-Chr-1681	Chlamydomonas reinhardtii	32.64	6e-22	97.1
LLPS-Viv-0198	Vitis vinifera	32.09	6e-29	116
LLPS-Amt-1866	Amborella trichopoda	31.97	4e-26	108
LLPS-Sem-0961	Selaginella moellendorffii	31.95	4e-33	127
LLPS-Brr-2543	Brassica rapa	31.85	3e-24	103
LLPS-Lem-1335	Leptosphaeria maculans	31.7	6e-25	105
LLPS-Hea-1449	Helianthus annuus	31.34	1e-26	110
LLPS-Nec-0760	Neurospora crassa	31.34	3e-21	94.7
LLPS-Abg-0452	Absidia glauca	31.23	3e-30	119
LLPS-Scc-0245	Schizosaccharomyces cryophilus	31.2	3e-25	105
LLPS-Scp-0945	Schizosaccharomyces pombe	31.13	8e-25	105
LLPS-Met-1655	Medicago truncatula	31.03	4e-23	100
LLPS-Prp-0293	Prunus persica	31.01	3e-26	108
LLPS-Aso-1319	Aspergillus oryzae	30.94	3e-23	100
LLPS-Asf-0675	Aspergillus flavus	30.94	3e-23	100
LLPS-Spr-1422	Sporisorium reilianum	30.92	2e-23	101
LLPS-Pug-0406	Puccinia graminis	30.83	2e-13	70.9
LLPS-Ast-1192	Aspergillus terreus	30.74	2e-24	103
LLPS-Sot-1526	Solanum tuberosum	30.68	7e-29	116
LLPS-Org-1452	Oryza glaberrima	30.57	7e-25	105
LLPS-Phn-1320	Phaeosphaeria nodorum	30.42	2e-26	109
LLPS-Php-0201	Physcomitrella patens	30.35	9e-33	127
LLPS-Sol-0031	Solanum lycopersicum	30.34	4e-23	100
LLPS-Miv-0963	Microbotryum violaceum	30.17	1e-19	90.1
LLPS-Arl-2742	Arabidopsis lyrata	30.08	2e-23	101
LLPS-Scj-0369	Schizosaccharomyces japonicus	29.8	1e-24	104
LLPS-Fus-1439	Fusarium solani	29.75	4e-23	100
LLPS-Nia-0276	Nicotiana attenuata	29.55	2e-27	112
LLPS-Mae-2592	Manihot esculenta	29.52	4e-26	108
LLPS-Sei-2356	Setaria italica	29.26	8e-24	102
LLPS-Blg-1580	Blumeria graminis	29.13	1e-22	99.0
LLPS-Drm-2381	Drosophila melanogaster	29.08	6e-25	104
LLPS-Glm-0566	Glycine max	29.01	2e-24	104
LLPS-Zem-1183	Zea mays	29.0	1e-19	90.1
LLPS-Beb-0756	Beauveria bassiana	28.99	1e-17	84.3
LLPS-Map-0234	Magnaporthe poae	28.99	2e-11	66.6
LLPS-Pyt-1341	Pyrenophora teres	28.98	3e-24	103
LLPS-Phv-1287	Phaseolus vulgaris	28.95	8e-20	90.5
LLPS-Fuo-1227	Fusarium oxysporum	28.82	2e-17	83.6
LLPS-Fuv-0790	Fusarium verticillioides	28.82	2e-17	83.6
LLPS-Asn-0504	Aspergillus nidulans	28.79	1e-17	84.0
LLPS-Orbr-0917	Oryza brachyantha	28.78	3e-26	108
LLPS-Zyt-1301	Zymoseptoria tritici	28.73	1e-23	101
LLPS-Nef-0677	Neosartorya fischeri	28.68	1e-19	90.5
LLPS-Asc-1120	Aspergillus clavatus	28.68	3e-23	100
LLPS-Cogr-1359	Colletotrichum graminicola	28.57	2e-17	83.6
LLPS-Asni-1420	Aspergillus niger	28.35	7e-20	90.9
LLPS-Asg-0848	Ashbya gossypii	28.26	1e-06	52.8
LLPS-Bro-2139	Brassica oleracea	28.12	2e-21	95.5
LLPS-Tum-1037	Tuber melanosporum	28.02	7e-20	91.3
LLPS-Crn-0387	Cryptococcus neoformans	27.94	3e-21	94.7
LLPS-Usm-0952	Ustilago maydis	27.9	2e-22	97.8
LLPS-Trv-0255	Trichoderma virens	27.83	2e-20	92.0
LLPS-Brn-3319	Brassica napus	27.8	2e-21	95.1
LLPS-Gor-1848	Gossypium raimondii	27.69	4e-22	97.4
LLPS-Asfu-0892	Aspergillus fumigatus	27.69	5e-20	91.3
LLPS-Art-2793	Arabidopsis thaliana	27.68	5e-24	102
LLPS-Lep-1859	Leersia perrieri	27.65	7e-19	88.2
LLPS-Brd-2345	Brachypodium distachyon	27.65	1e-19	90.1
LLPS-Sac-0225	Saccharomyces cerevisiae	27.55	3e-15	77.0
LLPS-Pot-0584	Populus trichocarpa	27.53	5e-24	102
LLPS-Mua-1745	Musa acuminata	27.52	8e-27	110
LLPS-Sob-1306	Sorghum bicolor	27.17	1e-23	102
LLPS-Put-0710	Puccinia triticina	26.97	4e-21	94.4
LLPS-Ori-1422	Oryza indica	26.83	7e-19	88.2
LLPS-Ved-0031	Verticillium dahliae	26.8	4e-17	82.8
LLPS-Thc-1986	Theobroma cacao	26.77	5e-22	97.1
LLPS-Cog-0569	Colletotrichum gloeosporioides	26.75	1e-09	61.2
LLPS-Orm-1479	Oryza meridionalis	26.69	3e-20	91.7
LLPS-Ors-2255	Oryza sativa	26.5	8e-20	90.9
LLPS-Orr-1083	Oryza rufipogon	26.5	8e-20	90.9
LLPS-Orb-1991	Oryza barthii	26.5	8e-20	90.5
LLPS-Orni-1224	Oryza nivara	26.5	8e-20	90.9
LLPS-Tra-2717	Triticum aestivum	26.49	8e-18	84.7
LLPS-Gag-1534	Gaeumannomyces graminis	26.38	3e-14	74.3
LLPS-Orgl-2074	Oryza glumaepatula	26.36	2e-18	86.7
LLPS-Osl-0512	Ostreococcus lucimarinus	26.09	4e-13	70.9
LLPS-Coc-2268	Corchorus capsularis	25.98	2e-18	86.7
LLPS-Via-2178	Vigna angularis	25.86	5e-14	73.6
LLPS-Orp-0630	Oryza punctata	25.78	1e-18	87.4
LLPS-Dac-0009	Daucus carota	25.0	3e-17	83.2
LLPS-Vir-1962	Vigna radiata	25.0	3e-16	79.7
LLPS-Tru-0811	Triticum urartu	24.9	1e-13	72.8
LLPS-Trr-0311	Trichoderma reesei	24.79	6e-19	87.8
LLPS-Mel-1526	Melampsora laricipopulina	23.94	1e-16	81.3

LLPS-Mum-4054Rpa2

Protein Sequence

Nucleotide Sequence

LLPS-Mum-4054
Rpa2