LLPS-Mum-2621
Runx3

▼ OVERVIEW

Status: Reviewed
Protein Name: Runt-related transcription factor 3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit
Gene Name: Runx3, Aml2, Cbfa3, Pebp2a3
Ensembl Gene: ENSMUSG00000070691.10
Ensembl Protein: ENSMUSP00000113159.1
Organism: Mus musculus
Taxa ID: 10090
LLPS Type: Others


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Centrosome/Spindle pole bodyPredicted from orthologsN/A(View)

▼ FUNCTION

Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-beta stimulation (By similarity). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation (PubMed:18258917). CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (PubMed:23481257).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MASNSIFDSF  PNYTPTFIRD  PSTSRRFTPP  STAFPCGGGG  GGKMGENSGA  LSAQATAGPG  60
61    GRTRPEVRSM  VDVLADHAGE  LVRTDSPNFL  CSVLPSHWRC  NKTLPVAFKV  VALGDVPDGT  120
121   VVTVMAGNDE  NYSAELRNAS  AVMKNQVARF  NDLRFVGRSG  RGKSFTLTIT  VFTNPTQVAT  180
181   YHRAIKVTVD  GPREPRRHRQ  KIEDQTKAFP  DRFGDLRMRV  TPSTPSPRGS  LSTTSHFSSQ  240
241   AQTPIQGSSD  LNPFSDPRQF  DRSFPTLQSL  TESRFPDPRM  HYPGAMSAAF  PYSATPSGTS  300
301   LGSLSVAGMP  ASSRFHHTYL  PPPYPGAPQS  QSGPFQANPA  PYHLFYGASS  GSYQFSMAAA  360
361   GGGERSPTRM  LTSCPSGASV  SAGNLMNPSL  GQADGVEADG  SHSNSPTALS  TPGRMDEAVW  420
421   RPY  423
Nucleotide CDS Sequence (FASTA)
1     ATGGCTTCCA  ACAGCATCTT  TGACTCCTTC  CCCAACTATA  CACCAACCTT  CATACGAGAC  60
61    CCGAGCACCA  GCCGCCGCTT  CACTCCCCCC  TCCACGGCCT  TCCCCTGCGG  CGGCGGCGGC  120
121   GGCGGCAAGA  TGGGCGAGAA  CAGCGGCGCG  CTAAGCGCGC  AGGCAACCGC  GGGCCCCGGC  180
181   GGCCGCACCC  GGCCCGAAGT  GCGCTCGATG  GTGGACGTGC  TGGCCGACCA  CGCGGGAGAG  240
241   CTCGTGCGCA  CCGACAGCCC  CAACTTCCTC  TGCTCCGTGC  TGCCCTCGCA  CTGGCGCTGC  300
301   AACAAGACGC  TGCCGGTCGC  CTTCAAGGTG  GTGGCCCTGG  GGGATGTGCC  GGATGGAACG  360
361   GTGGTGACCG  TGATGGCCGG  CAATGATGAG  AACTACTCCG  CCGAGCTGCG  CAACGCTTCC  420
421   GCTGTCATGA  AGAACCAAGT  GGCCAGGTTC  AACGACCTTC  GATTCGTGGG  CCGCAGTGGG  480
481   CGAGGGAAGA  GTTTCACGCT  CACAATCACC  GTGTTCACCA  ACCCTACCCA  AGTGGCTACC  540
541   TACCACCGAG  CCATCAAGGT  CACTGTGGAT  GGACCCCGGG  AACCCCGACG  GCACCGGCAG  600
601   AAGATAGAAG  ACCAGACCAA  GGCCTTCCCC  GACCGCTTTG  GAGACCTGCG  CATGCGTGTA  660
661   ACACCAAGCA  CACCCAGCCC  CCGTGGCTCT  CTCAGCACCA  CGAGCCACTT  CAGCAGCCAG  720
721   GCCCAGACCC  CAATCCAAGG  CTCCTCAGAC  CTGAACCCCT  TCTCCGACCC  CCGCCAGTTT  780
781   GACCGCTCCT  TCCCTACGCT  GCAGAGCCTC  ACAGAGAGCC  GCTTCCCGGA  CCCCAGGATG  840
841   CACTACCCGG  GAGCCATGTC  TGCCGCCTTC  CCCTACAGCG  CCACACCATC  GGGCACCAGC  900
901   CTGGGCAGCC  TGAGCGTGGC  GGGCATGCCG  GCCAGCAGCC  GCTTCCACCA  CACCTACCTC  960
961   CCTCCGCCCT  ACCCCGGGGC  CCCACAGAGC  CAGAGCGGGC  CCTTTCAGGC  CAACCCCGCG  1020
1021  CCCTACCACC  TCTTTTACGG  CGCCTCCTCC  GGCTCCTACC  AGTTCTCCAT  GGCAGCCGCG  1080
1081  GGAGGTGGTG  AGCGCTCGCC  CACCCGCATG  CTGACCTCCT  GCCCCAGCGG  CGCTTCGGTG  1140
1141  TCAGCAGGCA  ACCTCATGAA  CCCCAGCCTG  GGCCAGGCTG  ATGGCGTGGA  AGCCGACGGC  1200
1201  AGCCACAGCA  ACTCGCCCAC  GGCCCTGAGC  ACGCCGGGCC  GCATGGACGA  GGCCGTGTGG  1260
1261  CGGCCCTACT  AA  1272

▼ KEYWORD

ID
Family
Activator
Alternative splicing
Complete proteome
Cytoplasm
DNA-binding
Isopeptide bond
Nucleus
Phosphoprotein
Reference proteome
Transcription
Transcription regulation
Ubl conjugation

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Core-binding factor complex
Cellular Component
Cytoplasm
Cellular Component
Cytosol
Cellular Component
Intracellular membrane-bounded organelle
Cellular Component
Nuclear chromatin
Cellular Component
Nucleolus
Cellular Component
Nucleoplasm
Cellular Component
Nucleus
Molecular Function
ATP binding
Molecular Function
DNA binding
Molecular Function
DNA-binding transcription factor activity, RNA polymerase II-specific
Molecular Function
Histone deacetylase binding
Molecular Function
RNA polymerase II regulatory region sequence-specific DNA binding
Molecular Function
SMAD binding
Biological Process
Axon guidance
Biological Process
Cell maturation
Biological Process
Chondrocyte differentiation
Biological Process
Hair follicle morphogenesis
Biological Process
Hemopoiesis
Biological Process
Interferon-gamma production
Biological Process
MRNA transcription by RNA polymerase II
Biological Process
Negative regulation of CD4-positive, alpha-beta T cell differentiation
Biological Process
Negative regulation of cell cycle
Biological Process
Negative regulation of epithelial cell proliferation
Biological Process
Negative regulation of transcription by RNA polymerase II
Biological Process
Neuron differentiation
Biological Process
Neuron projection development
Biological Process
Positive regulation of CD8-positive, alpha-beta T cell differentiation
Biological Process
Positive regulation of extrinsic apoptotic signaling pathway
Biological Process
Positive regulation of transcription by RNA polymerase II
Biological Process
Positive regulation of transcription, DNA-templated
Biological Process
Protein phosphorylation
Biological Process
Response to transforming growth factor beta

▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Ora-1144Ornithorhynchus anatinus100.04e-43 152
LLPS-Mea-2403Mesocricetus auratus98.820.0 838
LLPS-Urm-0270Ursus maritimus94.444e-1473.6
LLPS-Ict-1385Ictidomys tridecemlineatus92.896e-145 426
LLPS-Dio-2412Dipodomys ordii92.720.0 593
LLPS-Poa-2275Pongo abelii91.410.0 664
LLPS-Rhb-0759Rhinopithecus bieti91.40.0 775
LLPS-Hos-1910Homo sapiens91.40.0 774
LLPS-Pap-3822Pan paniscus91.160.0 772
LLPS-Pat-2557Pan troglodytes91.160.0 772
LLPS-Aon-4409Aotus nancymaae90.930.0 771
LLPS-Fec-0292Felis catus90.930.0 774
LLPS-Gog-2356Gorilla gorilla90.70.0 768
LLPS-Caj-4206Callithrix jacchus90.70.0 771
LLPS-Maf-2193Macaca fascicularis90.70.0 771
LLPS-Chs-3313Chlorocebus sabaeus90.70.0 771
LLPS-Cea-0237Cercocebus atys90.70.0 771
LLPS-Paa-2102Papio anubis90.70.0 771
LLPS-Mam-1689Macaca mulatta90.70.0 771
LLPS-Man-4212Macaca nemestrina90.490.0 764
LLPS-Cap-3112Cavia porcellus90.320.0 761
LLPS-Tut-1758Tursiops truncatus90.230.0 766
LLPS-Sus-3002Sus scrofa89.950.0 728
LLPS-Nol-3080Nomascus leucogenys89.810.0 578
LLPS-Eqc-4351Equus caballus89.630.0 758
LLPS-Fud-1533Fukomys damarensis89.220.0 533
LLPS-Loa-2153Loxodonta africana87.960.0 746
LLPS-Bot-1352Bos taurus87.610.0 734
LLPS-Ova-1293Ovis aries86.960.0 515
LLPS-Lac-2221Latimeria chalumnae84.181e-98 299
LLPS-Anp-3093Anas platyrhynchos83.920.0 626
LLPS-Mod-2307Monodelphis domestica83.455e-155 447
LLPS-Tag-0371Taeniopygia guttata82.730.0 672
LLPS-Pes-0398Pelodiscus sinensis82.20.0 661
LLPS-Fia-0635Ficedula albicollis82.00.0 667
LLPS-Gaga-3092Gallus gallus81.980.0 571
LLPS-Myl-2231Myotis lucifugus81.620.0 588
LLPS-Sah-2304Sarcophilus harrisii81.130.0 518
LLPS-Mal-2100Mandrillus leucophaeus80.930.0 680
LLPS-Meg-0543Meleagris gallopavo78.071e-144 420
LLPS-Drm-1936Drosophila melanogaster76.863e-54 197
LLPS-Cii-1095Ciona intestinalis71.576e-38 147
LLPS-Cis-1246Ciona savignyi70.07e-68 219
LLPS-Ran-4131Rattus norvegicus69.038e-90 288
LLPS-Otg-2812Otolemur garnettii69.033e-90 289
LLPS-Orc-1862Oryctolagus cuniculus66.482e-60 204
LLPS-Leo-3371Lepisosteus oculatus64.990.0 544
LLPS-Xet-1334Xenopus tropicalis64.860.0 553
LLPS-Gaa-2272Gasterosteus aculeatus64.44e-162 470
LLPS-Ten-3035Tetraodon nigroviridis64.132e-173 499
LLPS-Xim-0966Xiphophorus maculatus63.949e-172 495
LLPS-Orn-3122Oreochromis niloticus63.937e-163 472
LLPS-Pof-0297Poecilia formosa63.863e-171 494
LLPS-Asm-1534Astyanax mexicanus63.823e-168 486
LLPS-Scm-2461Scophthalmus maximus63.292e-173 499
LLPS-Icp-0269Ictalurus punctatus62.867e-168 486
LLPS-Tar-0086Takifugu rubripes62.843e-168 486
LLPS-Orl-0455Oryzias latipes62.784e-163 473
LLPS-Dar-2025Danio rerio62.724e-170 491
LLPS-Anc-3556Anolis carolinensis60.055e-137 405
LLPS-Scf-1396Scleropages formosus59.099e-145 426
LLPS-Caf-1289Canis familiaris58.993e-48 172
LLPS-Mup-0357Mustela putorius furo54.731e-159 466
LLPS-Aim-0378Ailuropoda melanoleuca51.584e-118 360
LLPS-Cae-0781Caenorhabditis elegans51.541e-34 135