LLPS-Mum-0356
Khdrbs1

▼ OVERVIEW

Status: Reviewed
Protein Name: KH domain-containing, RNA-binding, signal transduction-associated protein 1; GAP-associated tyrosine phosphoprotein p62; Src-associated in mitosis 68 kDa protein; Sam68; p21 Ras GTPase-activating protein-associated p62; p68
Gene Name: Khdrbs1
Ensembl Gene: ENSMUSG00000028790.13
Ensembl Protein: ENSMUSP00000115402.1
Organism: Mus musculus
Taxa ID: 10090
LLPS Type: Others


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Chromatoid body
"...The list of 88 CB proteins identified in at least three separate experiments."
Round spermatids24554440
Postsynaptic density
"...We report the first direct comparison of the proteome of triplicate isolates of mouse and human cortical postsynaptic densities. The mouse postsynaptic density comprised 1556 proteins and the human one 1461."
Mouse cortex23071613
Sam68 nuclear body, Neuronal granule, Stress granulePredicted from orthologsN/A(View)

▼ FUNCTION

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. May not be involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species. RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). In cooperation with HNRNPA1 modulates alternative splicing of BCL2L1 by promoting splicing toward isoform Bcl-X(S), and of SMN1 (By similarity). Can regulate CD44 alternative splicing in a Ras pathway-dependent manner. Can regulate alternative splicing of NRXN1 and NRXN3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners. In a neuronal activity-dependent manner cooperates synergistically with KHDRBS2/SLIM-1 in regulation of NRXN1 exon skipping at AS4. The cooperation with KHDRBS2/SLIM-1 is antagonistic for regulation of NXRN3 alternative splicing at AS4 (PubMed:12478298, PubMed:22196734, PubMed:24469635).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MQRRDDPASR  LTRSSGRSCS  KDPSGAHPSV  RLTPSRPSPL  PHRPRGGGGG  PRGGARASPA  60
61    TQPPPLLPPS  TPGPDATVVG  SAPTPLLPPS  ATAAVKMEPE  NKYLPELMAE  KDSLDPSFTH  120
121   AMQLLSVEIE  KIQKGESKKD  DEENYLDLFS  HKNMKLKERV  LIPVKQYPKF  NFVGKILGPQ  180
181   GNTIKRLQEE  TGAKISVLGK  GSMRDKAKEE  ELRKGGDPKY  AHLNMDLHVF  IEVFGPPCEA  240
241   YALMAHAMEE  VKKFLVPDMM  DDICQEQFLE  LSYLNGVPEP  SRGRGVSVRG  RGAAPPPPPV  300
301   PRGRGVGPPR  GALVRGTPVR  GSITRGATVT  RGVPPPPTVR  GAPTPRARTA  GIQRIPLPPT  360
361   PAPETYEDYG  YDDTYAEQSY  EGYEGYYSQS  QGESEYYDYG  HGELQDSYEA  YGQDDWNGTR  420
421   PSLKAPPARP  VKGAYREHPY  GRY  443
Nucleotide CDS Sequence (FASTA)
1     ATGCAGCGCC  GGGACGATCC  TGCCTCGCGC  CTCACCCGGT  CCTCGGGCCG  CAGCTGCTCC  60
61    AAGGACCCGT  CAGGTGCCCA  CCCCTCGGTG  CGTCTGACCC  CGTCTCGGCC  GTCGCCGCTT  120
121   CCTCACCGGC  CCCGGGGAGG  GGGAGGTGGG  CCCAGAGGAG  GCGCTCGGGC  CTCGCCCGCC  180
181   ACCCAGCCGC  CGCCGCTGCT  GCCTCCCTCC  ACCCCTGGTC  CCGACGCGAC  GGTGGTGGGT  240
241   TCCGCGCCGA  CCCCGCTGCT  GCCCCCGTCA  GCCACAGCCG  CGGTCAAGAT  GGAGCCGGAG  300
301   AATAAGTACC  TGCCTGAACT  CATGGCCGAG  AAGGACTCGC  TCGACCCGTC  CTTCACTCAC  360
361   GCCATGCAGC  TGCTGTCCGT  AGAAATTGAG  AAGATTCAGA  AGGGAGAGTC  AAAAAAAGAT  420
421   GACGAGGAGA  ATTATTTGGA  TTTATTTTCT  CATAAGAACA  TGAAGCTGAA  AGAACGCGTG  480
481   CTGATACCTG  TCAAGCAGTA  TCCAAAGTTC  AATTTTGTGG  GGAAGATTCT  TGGACCACAA  540
541   GGAAATACAA  TCAAAAGACT  CCAGGAAGAG  ACTGGTGCAA  AGATCTCTGT  CTTGGGGAAG  600
601   GGTTCAATGA  GAGACAAAGC  CAAGGAGGAA  GAGTTGCGCA  AGGGTGGAGA  CCCCAAATAT  660
661   GCCCATTTAA  ATATGGATCT  GCATGTCTTC  ATTGAAGTCT  TTGGACCCCC  GTGTGAAGCT  720
721   TATGCTCTTA  TGGCCCATGC  TATGGAAGAA  GTCAAGAAGT  TCCTAGTACC  AGATATGATG  780
781   GATGATATCT  GTCAGGAGCA  GTTTCTAGAA  TTGTCCTACT  TGAACGGAGT  ACCTGAACCC  840
841   TCTCGTGGTC  GTGGGGTATC  TGTGAGAGGA  CGAGGAGCTG  CCCCTCCTCC  TCCACCTGTT  900
901   CCCAGAGGAC  GTGGTGTTGG  ACCACCTAGA  GGAGCTTTGG  TTCGTGGAAC  CCCAGTGAGA  960
961   GGCTCCATCA  CCAGAGGTGC  CACTGTGACT  CGAGGAGTGC  CACCCCCACC  TACTGTGAGG  1020
1021  GGTGCTCCAA  CACCAAGAGC  TCGGACAGCT  GGGATTCAGA  GAATACCTTT  GCCTCCCACA  1080
1081  CCTGCACCAG  AAACATACGA  AGATTATGGA  TATGATGATA  CATACGCAGA  ACAGAGTTAC  1140
1141  GAAGGCTATG  AAGGCTATTA  CAGCCAGAGT  CAAGGGGAGT  CAGAGTATTA  TGACTATGGA  1200
1201  CATGGGGAGC  TCCAAGATTC  TTACGAAGCC  TACGGACAAG  ATGACTGGAA  TGGGACCAGG  1260
1261  CCATCACTGA  AGGCTCCTCC  AGCTAGGCCA  GTGAAGGGAG  CATACAGAGA  GCATCCATAT  1320
1321  GGACGTTATT  AA  1332

▼ KEYWORD

ID
Family
Acetylation
Cell cycle
Complete proteome
Cytoplasm
Direct protein sequencing
Isopeptide bond
Membrane
Methylation
mRNA processing
Nucleus
Phosphoprotein
Reference proteome
RNA-binding
SH3-binding
Transcription
Transcription regulation
Ubl conjugation

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cytoplasm
Cellular Component
Grb2-Sos complex
Cellular Component
Membrane
Cellular Component
Nucleoplasm
Cellular Component
Nucleus
Molecular Function
Identical protein binding
Molecular Function
Poly(A) binding
Molecular Function
Poly(U) RNA binding
Molecular Function
Protein domain specific binding
Molecular Function
Protein-containing complex binding
Molecular Function
RNA binding
Molecular Function
SH3 domain binding
Molecular Function
SH3/SH2 adaptor activity
Biological Process
Cell cycle
Biological Process
Cell surface receptor signaling pathway
Biological Process
MRNA processing
Biological Process
Negative regulation of transcription, DNA-templated
Biological Process
Positive regulation of RNA export from nucleus
Biological Process
Positive regulation of translational initiation
Biological Process
Protein complex oligomerization
Biological Process
Regulation of alternative mRNA splicing, via spliceosome
Biological Process
Regulation of mRNA splicing, via spliceosome
Biological Process
Regulation of RNA export from nucleus
Biological Process
Spermatogenesis

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Ran-0026Rattus norvegicus98.870.0 545
LLPS-Gaga-2029Gallus gallus97.865e-124 375
LLPS-Ora-3528Ornithorhynchus anatinus97.835e-24 100
LLPS-Mal-0923Mandrillus leucophaeus97.134e-159 461
LLPS-Urm-1465Ursus maritimus97.125e-158 458
LLPS-Tag-1796Taeniopygia guttata96.14e-99 301
LLPS-Nol-0983Nomascus leucogenys95.672e-133 397
LLPS-Meg-1467Meleagris gallopavo95.035e-101 311
LLPS-Anp-1886Anas platyrhynchos95.037e-101 311
LLPS-Orc-2262Oryctolagus cuniculus95.037e-175 504
LLPS-Ict-0462Ictidomys tridecemlineatus94.818e-175 504
LLPS-Pap-0669Pan paniscus94.364e-166 482
LLPS-Pat-1043Pan troglodytes94.364e-166 482
LLPS-Hos-2491Homo sapiens94.364e-166 482
LLPS-Man-0141Macaca nemestrina94.364e-166 482
LLPS-Rhb-0067Rhinopithecus bieti94.364e-166 482
LLPS-Poa-0311Pongo abelii94.367e-165 483
LLPS-Maf-1541Macaca fascicularis94.364e-166 482
LLPS-Chs-4062Chlorocebus sabaeus94.365e-165 483
LLPS-Aon-1372Aotus nancymaae94.364e-166 482
LLPS-Cea-1667Cercocebus atys94.364e-166 482
LLPS-Gog-2947Gorilla gorilla94.364e-166 482
LLPS-Caj-1902Callithrix jacchus94.361e-164 483
LLPS-Paa-0500Papio anubis94.367e-165 483
LLPS-Fec-4043Felis catus94.132e-165 480
LLPS-Eqc-2090Equus caballus94.134e-165 480
LLPS-Sus-0446Sus scrofa94.132e-165 480
LLPS-Tut-0144Tursiops truncatus94.132e-165 480
LLPS-Myl-2343Myotis lucifugus94.131e-166 484
LLPS-Mup-3016Mustela putorius furo94.132e-165 480
LLPS-Mod-0593Monodelphis domestica93.989e-156 456
LLPS-Bot-3098Bos taurus93.913e-165 480
LLPS-Caf-2151Canis familiaris92.674e-158 462
LLPS-Otg-1112Otolemur garnettii92.344e-164 477
LLPS-Fia-1889Ficedula albicollis91.33e-2198.6
LLPS-Ova-0962Ovis aries90.264e-137 408
LLPS-Lac-2862Latimeria chalumnae89.541e-93 292
LLPS-Pes-0426Pelodiscus sinensis89.416e-131 388
LLPS-Cap-3720Cavia porcellus89.174e-167 486
LLPS-Dio-0572Dipodomys ordii89.168e-173 499
LLPS-Aim-1656Ailuropoda melanoleuca89.161e-164 478
LLPS-Mam-2742Macaca mulatta85.551e-128 385
LLPS-Leo-2621Lepisosteus oculatus85.418e-106 325
LLPS-Orl-0798Oryzias latipes83.552e-81 262
LLPS-Orn-1495Oreochromis niloticus82.876e-99 308
LLPS-Scf-2833Scleropages formosus82.872e-99 311
LLPS-Pof-1200Poecilia formosa81.975e-98 305
LLPS-Xim-1347Xiphophorus maculatus81.973e-98 306
LLPS-Tar-0189Takifugu rubripes81.975e-98 305
LLPS-Ten-1486Tetraodon nigroviridis81.623e-99 308
LLPS-Anc-0012Anolis carolinensis81.383e-135 400
LLPS-Mea-1382Mesocricetus auratus81.049e-109 334
LLPS-Scm-2960Scophthalmus maximus80.877e-97 302
LLPS-Dar-3549Danio rerio80.452e-97 303
LLPS-Icp-1513Ictalurus punctatus80.333e-97 303
LLPS-Asm-3228Astyanax mexicanus78.892e-94 296
LLPS-Loa-1321Loxodonta africana74.722e-85 272
LLPS-Ere-0819Erinaceus europaeus74.724e-85 270
LLPS-Xet-0337Xenopus tropicalis74.222e-132 394
LLPS-Sah-2092Sarcophilus harrisii73.172e-32 128
LLPS-Fud-1595Fukomys damarensis72.482e-68 226
LLPS-Gaa-2033Gasterosteus aculeatus71.23e-79 257
LLPS-Cas-3249Carlito syrichta70.21e-65 219
LLPS-Drm-1017Drosophila melanogaster48.853e-47 173
LLPS-Cis-0413Ciona savignyi45.784e-40 152
LLPS-Tra-2256Triticum aestivum45.163e-22 100
LLPS-Cii-2008Ciona intestinalis44.878e-38 140
LLPS-Coc-1978Corchorus capsularis43.74e-2298.2
LLPS-Lep-1345Leersia perrieri42.862e-2299.0
LLPS-Brr-1786Brassica rapa42.024e-2299.0
LLPS-Php-1856Physcomitrella patens39.684e-2299.8
LLPS-Cae-0169Caenorhabditis elegans34.552e-25 112