DrLLPS - Data resource of LLPS

LLPS-Man-3991
APOE

Integrated Annotations

▼ OVERVIEW


Status:	Unreviewed
Protein Name:	Apolipoprotein E
Gene Name:	APOE
Ensembl Gene:	ENSMNEG00000032951.1
Ensembl Protein:	ENSMNEP00000017988.1
Organism:	Macaca nemestrina
Taxa ID:	9545
LLPS Type:	LLPS regulator

▼ PROPERTY

——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:

Condensate	Evidence	Orthologs
Stress granule, Postsynaptic density	Predicted from orthologs	(View)

Organism	DrLLPS ID	UniProt ID	LLPS type	Condensate	Identity	E-value	Score
Homo sapiens	LLPS-Hos-0967	P02649	Regulator	Stress granule, Postsynaptic density	93.69	0.0	512
Mus musculus	LLPS-Mum-3610	P08226	Client	Postsynaptic density	71.05	3e-140	406

▼ FUNCTION

APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells. A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes. APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues. By participating to the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis. APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis. First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues. Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes. APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting.

▼ CROSS REFERENCE

Database	Nucleotide ID	Protein ID
Ensembl	ENSMNET00000042220.1	ENSMNEP00000017988.1
UniProt		P0DO94, APOE_MACNE
GeneBank	JZLF01080368

▼ SEQUENCE

Protein Sequence (FASTA)
1 MKVLWAALLV TFLAGCQAKV EQPVEPETEP ELRQQAEGQS GQPWELALGR FWDYLRWVQT 60 61 LSEQVQEELL SPQVTQELTT LMDETMKELK AYKSELEEQL SPVAEETRAR LSKELQAAQA 120 121 RLGADMEDVR SRLVQYRSEV QAMLGQSTEE LRARLASHLR KLRKRLLRDA DDLQKRLAVY 180 181 QAGAREGAER GVSAIRERLG PLVEQGRVRA ATVGSLASQP LQERAQALGE RLRARMEEMG 240 241 SRTRDRLDEV KEQVAEVRAK LEEQAQQISL QAEAFQARLK SWFEPLVEDM QRQWAGLVEK 300 301 VQAAVGASTA PVPSDNH 317
Nucleotide CDS Sequence (FASTA)
1 ATGAAGGTTC TGTGGGCTGC GTTGCTGGTC ACATTCCTGG CAGGATGCCA GGCCAAGGTG 60 61 GAGCAACCGG TGGAGCCAGA GACGGAACCC GAGCTTCGCC AGCAGGCTGA GGGGCAGAGC 120 121 GGCCAGCCCT GGGAGCTGGC ACTGGGTCGC TTTTGGGATT ACCTGCGCTG GGTGCAGACA 180 181 CTGTCTGAGC AGGTGCAGGA GGAGCTGCTC AGCCCCCAGG TCACCCAGGA ACTGACGACG 240 241 CTGATGGATG AGACCATGAA GGAGTTGAAG GCCTACAAAT CGGAACTGGA GGAACAGCTG 300 301 AGCCCGGTGG CGGAGGAGAC GCGGGCACGG CTGTCCAAGG AGCTGCAGGC GGCGCAGGCC 360 361 CGGCTGGGTG CCGACATGGA GGACGTGCGC AGCCGCCTGG TGCAGTACCG CAGCGAGGTG 420 421 CAGGCCATGC TGGGCCAGAG TACCGAGGAG CTGCGGGCGC GCCTCGCCTC CCACCTGCGC 480 481 AAGCTGCGCA AGCGGCTCCT CCGCGATGCT GATGACCTGC AGAAGCGCCT GGCAGTGTAT 540 541 CAGGCCGGGG CCCGCGAGGG CGCCGAGCGC GGGGTCAGCG CCATCCGCGA GCGCCTGGGA 600 601 CCCCTGGTGG AGCAGGGCCG CGTGCGGGCC GCCACTGTGG GCTCCCTGGC CAGCCAGCCG 660 661 CTTCAGGAGC GGGCCCAGGC CTTGGGTGAG CGGCTTCGCG CACGGATGGA GGAGATGGGC 720 721 AGCCGGACCC GCGACCGCCT GGACGAGGTG AAGGAGCAGG TGGCGGAGGT GCGCGCCAAG 780 781 CTGGAGGAAC AGGCCCAGCA GATAAGCCTG CAGGCCGAGG CCTTCCAGGC CCGCCTCAAG 840 841 AGCTGGTTCG AGCCCCTGGT GGAAGACATG CAGCGCCAGT GGGCTGGGCT GGTGGAGAAG 900 901 GTGCAGGCTG CCGTGGGCGC CAGCACTGCC CCTGTGCCCA GCGACAATCA CTGA 954

▼ KEYWORD

ID	Family
KW-0162	Chylomicron
KW-0181	Complete proteome
KW-0272	Extracellular matrix
KW-0325	Glycoprotein
KW-0345	HDL
KW-0358	Heparin-binding
KW-0445	Lipid transport
KW-0446	Lipid-binding
KW-0558	Oxidation
KW-0597	Phosphoprotein
KW-1185	Reference proteome
KW-0677	Repeat
KW-0964	Secreted
KW-0732	Signal
KW-0813	Transport
KW-0850	VLDL

▼ GENE ONTOLOGY

ID	Classification	Description
GO:0042627	Cellular Component	Chylomicron
GO:0005783	Cellular Component	Endoplasmic reticulum
GO:0031012	Cellular Component	Extracellular matrix
GO:0005615	Cellular Component	Extracellular space
GO:0098978	Cellular Component	Glutamatergic synapse
GO:0005794	Cellular Component	Golgi apparatus
GO:0034364	Cellular Component	High-density lipoprotein particle
GO:0034363	Cellular Component	Intermediate-density lipoprotein particle
GO:0034362	Cellular Component	Low-density lipoprotein particle
GO:0005886	Cellular Component	Plasma membrane
GO:0043083	Cellular Component	Synaptic cleft
GO:0034361	Cellular Component	Very-low-density lipoprotein particle
GO:0001540	Molecular Function	Amyloid-beta binding
GO:0016209	Molecular Function	Antioxidant activity
GO:0017127	Molecular Function	Cholesterol transporter activity
GO:0043395	Molecular Function	Heparan sulfate proteoglycan binding
GO:0008201	Molecular Function	Heparin binding
GO:0042802	Molecular Function	Identical protein binding
GO:0071813	Molecular Function	Lipoprotein particle binding
GO:0050750	Molecular Function	Low-density lipoprotein particle receptor binding
GO:0046911	Molecular Function	Metal chelating activity
GO:0060228	Molecular Function	Phosphatidylcholine-sterol O-acyltransferase activator activity
GO:0005543	Molecular Function	Phospholipid binding
GO:0042803	Molecular Function	Protein homodimerization activity
GO:0048156	Molecular Function	Tau protein binding
GO:0070326	Molecular Function	Very-low-density lipoprotein particle receptor binding
GO:0097113	Biological Process	AMPA glutamate receptor clustering
GO:0042982	Biological Process	Amyloid precursor protein metabolic process
GO:0048844	Biological Process	Artery morphogenesis
GO:0006874	Biological Process	Cellular calcium ion homeostasis
GO:0019934	Biological Process	CGMP-mediated signaling
GO:0006707	Biological Process	Cholesterol catabolic process
GO:0033344	Biological Process	Cholesterol efflux
GO:0042632	Biological Process	Cholesterol homeostasis
GO:0034382	Biological Process	Chylomicron remnant clearance
GO:0055089	Biological Process	Fatty acid homeostasis
GO:0007186	Biological Process	G protein-coupled receptor signaling pathway
GO:0034380	Biological Process	High-density lipoprotein particle assembly
GO:0034384	Biological Process	High-density lipoprotein particle clearance
GO:0034375	Biological Process	High-density lipoprotein particle remodeling
GO:0071831	Biological Process	Intermediate-density lipoprotein particle clearance
GO:0010877	Biological Process	Lipid transport involved in lipid storage
GO:0042158	Biological Process	Lipoprotein biosynthetic process
GO:0042159	Biological Process	Lipoprotein catabolic process
GO:0035641	Biological Process	Locomotory exploration behavior
GO:0015909	Biological Process	Long-chain fatty acid transport
GO:0007616	Biological Process	Long-term memory
GO:0034374	Biological Process	Low-density lipoprotein particle remodeling
GO:0051651	Biological Process	Maintenance of location in cell
GO:1902430	Biological Process	Negative regulation of amyloid-beta formation
GO:0043537	Biological Process	Negative regulation of blood vessel endothelial cell migration
GO:0090090	Biological Process	Negative regulation of canonical Wnt signaling pathway
GO:0045541	Biological Process	Negative regulation of cholesterol biosynthetic process
GO:0001937	Biological Process	Negative regulation of endothelial cell proliferation
GO:0010629	Biological Process	Negative regulation of gene expression
GO:0050728	Biological Process	Negative regulation of inflammatory response
GO:1900272	Biological Process	Negative regulation of long-term synaptic potentiation
GO:0043407	Biological Process	Negative regulation of MAP kinase activity
GO:1901215	Biological Process	Negative regulation of neuron death
GO:0010977	Biological Process	Negative regulation of neuron projection development
GO:0010544	Biological Process	Negative regulation of platelet activation
GO:1901630	Biological Process	Negative regulation of presynaptic membrane organization
GO:0090209	Biological Process	Negative regulation of triglyceride metabolic process
GO:0031175	Biological Process	Neuron projection development
GO:0007263	Biological Process	Nitric oxide mediated signal transduction
GO:0097114	Biological Process	NMDA glutamate receptor clustering
GO:0033700	Biological Process	Phospholipid efflux
GO:0044794	Biological Process	Positive regulation by host of viral process
GO:0010875	Biological Process	Positive regulation of cholesterol efflux
GO:0010873	Biological Process	Positive regulation of cholesterol esterification
GO:0060999	Biological Process	Positive regulation of dendritic spine development
GO:1902952	Biological Process	Positive regulation of dendritic spine maintenance
GO:0045807	Biological Process	Positive regulation of endocytosis
GO:0070374	Biological Process	Positive regulation of ERK1 and ERK2 cascade
GO:1905855	Biological Process	Positive regulation of heparan sulfate binding
GO:1905860	Biological Process	Positive regulation of heparan sulfate proteoglycan binding
GO:0046889	Biological Process	Positive regulation of lipid biosynthetic process
GO:1903002	Biological Process	Positive regulation of lipid transport across blood-brain barrier
GO:0032805	Biological Process	Positive regulation of low-density lipoprotein particle receptor catabolic process
GO:0051044	Biological Process	Positive regulation of membrane protein ectodomain proteolysis
GO:0051000	Biological Process	Positive regulation of nitric-oxide synthase activity
GO:1902995	Biological Process	Positive regulation of phospholipid efflux
GO:1901628	Biological Process	Positive regulation of postsynaptic membrane organization
GO:0045893	Biological Process	Positive regulation of transcription, DNA-templated
GO:0017038	Biological Process	Protein import
GO:0006898	Biological Process	Receptor-mediated endocytosis
GO:1905906	Biological Process	Regulation of amyloid fibril formation
GO:1900221	Biological Process	Regulation of amyloid-beta clearance
GO:0042981	Biological Process	Regulation of apoptotic process
GO:2000822	Biological Process	Regulation of behavioral fear response
GO:0032489	Biological Process	Regulation of Cdc42 protein signal transduction
GO:1905890	Biological Process	Regulation of cellular response to very-low-density lipoprotein particle stimulus
GO:0045088	Biological Process	Regulation of innate immune response
GO:0061136	Biological Process	Regulation of proteasomal protein catabolic process
GO:0032462	Biological Process	Regulation of protein homooligomerization
GO:0061771	Biological Process	Response to caloric restriction
GO:0002021	Biological Process	Response to dietary excess
GO:0006979	Biological Process	Response to oxidative stress
GO:0043691	Biological Process	Reverse cholesterol transport
GO:0070328	Biological Process	Triglyceride homeostasis
GO:0006641	Biological Process	Triglyceride metabolic process
GO:0071830	Biological Process	Triglyceride-rich lipoprotein particle clearance
GO:0042311	Biological Process	Vasodilation
GO:0034447	Biological Process	Very-low-density lipoprotein particle clearance
GO:0034372	Biological Process	Very-low-density lipoprotein particle remodeling
GO:0019068	Biological Process	Virion assembly

▼ ORTHOLOGY

DrLLPS ID	Organism	Identity	E-value	Score
LLPS-Mam-1368	Macaca mulatta	100.0	0.0	527
LLPS-Maf-1827	Macaca fascicularis	100.0	0.0	527
LLPS-Paa-3152	Papio anubis	99.68	0.0	523
LLPS-Chs-0229	Chlorocebus sabaeus	99.05	0.0	518
LLPS-Cea-2931	Cercocebus atys	98.42	0.0	516
LLPS-Rhb-0795	Rhinopithecus bieti	97.48	0.0	511
LLPS-Pap-2285	Pan paniscus	94.32	3e-174	493
LLPS-Pat-1114	Pan troglodytes	94.32	3e-174	493
LLPS-Poa-4468	Pongo abelii	94.32	1e-173	491
LLPS-Hos-0967	Homo sapiens	93.69	2e-172	488
LLPS-Caj-2708	Callithrix jacchus	90.62	2e-168	478
LLPS-Nol-0037	Nomascus leucogenys	87.07	9e-149	428
LLPS-Otg-4113	Otolemur garnettii	81.45	7e-145	418
LLPS-Bot-1420	Bos taurus	75.0	4e-122	361
LLPS-Ran-4859	Rattus norvegicus	74.04	4e-123	364
LLPS-Fec-3304	Felis catus	73.85	3e-106	319
LLPS-Mup-0859	Mustela putorius furo	73.67	5e-129	378
LLPS-Tut-1541	Tursiops truncatus	73.5	5e-131	383
LLPS-Sus-2619	Sus scrofa	73.18	1e-124	367
LLPS-Mea-1347	Mesocricetus auratus	72.93	2e-132	387
LLPS-Aon-1141	Aotus nancymaae	72.31	1e-130	380
LLPS-Cap-0067	Cavia porcellus	72.29	8e-125	366
LLPS-Eqc-1143	Equus caballus	72.24	4e-127	373
LLPS-Ova-4225	Ovis aries	70.98	2e-124	366
LLPS-Mum-3610	Mus musculus	70.13	8e-131	382
LLPS-Loa-3477	Loxodonta africana	70.03	1e-125	369
LLPS-Caf-1692	Canis familiaris	69.91	3e-122	361
LLPS-Fud-4360	Fukomys damarensis	68.0	3e-58	193
LLPS-Aim-0812	Ailuropoda melanoleuca	66.78	3e-105	317
LLPS-Myl-2614	Myotis lucifugus	64.89	6e-111	332
LLPS-Dio-3677	Dipodomys ordii	64.47	6e-107	320
LLPS-Mod-2122	Monodelphis domestica	48.74	3e-59	198
LLPS-Sah-3036	Sarcophilus harrisii	45.76	2e-40	147
LLPS-Ora-3487	Ornithorhynchus anatinus	41.44	8e-51	176
LLPS-Leo-2501	Lepisosteus oculatus	32.71	2e-13	71.2
LLPS-Dar-3412	Danio rerio	29.14	3e-21	95.5
LLPS-Orn-2719	Oreochromis niloticus	29.11	6e-24	103
LLPS-Gaa-0219	Gasterosteus aculeatus	28.27	2e-20	93.2
LLPS-Tar-2210	Takifugu rubripes	27.71	2e-18	87.0
LLPS-Lac-3372	Latimeria chalumnae	27.51	6e-27	112
LLPS-Scm-0225	Scophthalmus maximus	27.04	2e-21	95.9
LLPS-Scf-1952	Scleropages formosus	26.64	5e-24	103
LLPS-Asm-2917	Astyanax mexicanus	26.49	1e-21	96.7
LLPS-Icp-2604	Ictalurus punctatus	26.03	1e-20	94.0
LLPS-Xet-2377	Xenopus tropicalis	24.71	1e-18	87.8

LLPS-Man-3991APOE

Protein Sequence

Nucleotide Sequence

LLPS-Man-3991
APOE