LLPS-Hos-a172
▼ Classification
Condensates:
| Condensate | Description | Tissue/Cell | PMIDs |
|---|---|---|---|
| Nucleolus | "...The data set from all our nucleolar mass spectrometry analyses defines an updated group of 692 proteins that reproducibly copurify with human nucleoli." | HeLa cells | 15635413 |
▼ FUNCTION
| Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | ENST00000361690.7 | ENSP00000354674.3 |
| Ensembl | ENST00000373825.6 | ENSP00000362931.2 |
| Ensembl | ENST00000510290.5 | ENSP00000424170.1 |
| Ensembl | ENST00000507292.1 | ENSP00000422886.1 |
| Ensembl | ENST00000508473.5 | ENSP00000425172.1 |
| Ensembl | ENST00000423325.6 | ENSP00000391069.2 |
| Ensembl | ENST00000507909.1 | ENSP00000424323.1 |
| Ensembl | ENST00000512445.5 | ENSP00000424068.1 |
| GeneBank | U09564, AJ318054, AK299591, Z99128, CH471081, BC038292 | AAA20530.1, CAC39299.1, BAG61523.1, EAX03859.1, AAH38292.1 |
| Entrez | 6732 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 KGERWSGLRH EGQWSPGRGP VLALQARKKR TKAKKDKAQR KSETQHRGSA PHSESDLPEQ 60 61 EEEILGSDDD EQEDPNDYCK GGYHLVKIGD LFNGRYHVIR KLGWGHFSTV WLSWDIQGKK 120 121 FVAMKVVKSA EHYTETALDE IRLLKSVRNS DPNDPNREMV VQLLDDFKIS GVNGTHICMV 180 181 FEVLGHHLLK WIIKSNYQGL PLPCVKKIIQ QVLQGLDYLH TKCRIIHTDI KPENILLSVN 240 241 EQYIRRLAAE ATEWQRSGAP PPSGSAVSTA PQPKPADKMS KNKKKKLKKK QKRQAELLEK 300 301 RMQEIEEMEK ESGPGQKRPN KQEESESPVE RPLKENPPNK MTQEKLEESS TIGQDQTLME 360 361 RDTEGGAAEI NCNGVIEVIN YTQNSNNETL RHKEDLHNAN DCDVQNLNQE SSFLSSQNGD 420 421 SSTSQETDSC TPITSEVSDT MVCQSSSTVG QSFSEQHISQ LQESIRAEIP CEDEQEQEHN 480 481 GPLDNKGKST AGNFLVNPLE PKNAEKLKVK IADLGNACWV HKHFTEDIQT RQYRSLEVLI 540 541 GSGYNTPADI WSTACMAFEL ATGDYLFEPH SGEEYTRDED HIALIIELLG KVPRKLIVAG 600 601 KYSKEFFTKK GDLKHITKLK PWGLFEVLVE KYEWSQEEAA GFTDFLLPML ELIPEKRATA 660 661 AECLRHPWLN S 671 |
| Nucleotide CDS Sequence (FASTA) |
1 Sequence u navailable 20 |
▼ KEYWORD
ID | Family |
3D-structure | |
Alternative splicing | |
ATP-binding | |
Chromosome partition | |
Complete proteome | |
Cytoplasm | |
Differentiation | |
Direct protein sequencing | |
Endoplasmic reticulum | |
Host-virus interaction | |
Kinase | |
Microsome | |
mRNA processing | |
mRNA splicing | |
Nucleotide-binding | |
Nucleus | |
Phosphoprotein | |
Polymorphism | |
Reference proteome | |
Serine/threonine-protein kinase | |
Transferase
|
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Cytoplasm | |
Cellular Component | Cytosol | |
Cellular Component | Endoplasmic reticulum | |
Cellular Component | Nuclear matrix | |
Cellular Component | Nucleus | |
Cellular Component | Plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | Magnesium ion binding | |
Molecular Function | Protein kinase activity | |
Molecular Function | Protein serine/threonine kinase activity | |
Molecular Function | RNA binding | |
Biological Process | Chromosome segregation | |
Biological Process | Innate immune response | |
Biological Process | Intracellular signal transduction | |
Biological Process | Negative regulation of viral genome replication | |
Biological Process | Positive regulation of viral genome replication | |
Biological Process | Protein phosphorylation | |
Biological Process | Regulation of gene expression | |
Biological Process | Regulation of mRNA processing | |
Biological Process | Regulation of mRNA splicing, via spliceosome | |
Biological Process | RNA splicing | |
Biological Process | Sperm chromatin condensation | |
Biological Process | Spliceosomal complex assembly | |
Biological Process | Viral process |
▼ ANNOTATION
| Disorder | ||||
| IUPred2A | ||||
| PTM | ||||
| BioGRID | ||||
| Interaction | ||||
| RISE | RAIN | MIST | DifferentialNET | |
| Physicochemical | ||||
| AAindex | ||||
| Function | ||||
| THANATOS | ||||
| Proteomics | ||||
| THPA | ||||
| Localization | ||||
| COMPARTMENTS | ||||
| Expression | ||||
| TCGA | ArrayExpress | TISSUES | THPA | |
| Methylation | ||||
| TCGA | MethyCancer | |||
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Gog-a169 | Gorilla gorilla | 100.0 | 0.0 | 1363 |
| LLPS-Poa-a169 | Pongo abelii | 99.85 | 0.0 | 1360 |
| LLPS-Pap-a168 | Pan paniscus | 99.85 | 0.0 | 1351 |
| LLPS-Pat-a168 | Pan troglodytes | 99.69 | 0.0 | 1360 |
| LLPS-Chs-4484 | Chlorocebus sabaeus | 99.39 | 0.0 | 1344 |
| LLPS-Maf-4027 | Macaca fascicularis | 99.39 | 0.0 | 1354 |
| LLPS-Paa-a172 | Papio anubis | 99.39 | 0.0 | 1354 |
| LLPS-Man-a170 | Macaca nemestrina | 99.24 | 0.0 | 1354 |
| LLPS-Mam-3941 | Macaca mulatta | 99.23 | 0.0 | 1343 |
| LLPS-Mal-a168 | Mandrillus leucophaeus | 98.92 | 0.0 | 1336 |
| LLPS-Aon-a173 | Aotus nancymaae | 98.77 | 0.0 | 1337 |
| LLPS-Caj-a168 | Callithrix jacchus | 98.47 | 0.0 | 1345 |
| LLPS-Cea-4461 | Cercocebus atys | 97.24 | 0.0 | 1303 |
| LLPS-Otg-a172 | Otolemur garnettii | 96.99 | 0.0 | 1281 |
| LLPS-Aim-a165 | Ailuropoda melanoleuca | 96.77 | 0.0 | 1310 |
| LLPS-Orc-a166 | Oryctolagus cuniculus | 96.66 | 0.0 | 1319 |
| LLPS-Nol-a175 | Nomascus leucogenys | 96.65 | 0.0 | 1316 |
| LLPS-Mup-a172 | Mustela putorius furo | 96.64 | 0.0 | 1320 |
| LLPS-Fec-a171 | Felis catus | 96.62 | 0.0 | 1305 |
| LLPS-Caf-2757 | Canis familiaris | 96.49 | 0.0 | 1310 |
| LLPS-Sus-0795 | Sus scrofa | 96.49 | 0.0 | 1317 |
| LLPS-Rhb-4750 | Rhinopithecus bieti | 96.49 | 0.0 | 1308 |
| LLPS-Ict-a170 | Ictidomys tridecemlineatus | 96.34 | 0.0 | 1315 |
| LLPS-Urm-0474 | Ursus maritimus | 96.17 | 0.0 | 1064 |
| LLPS-Ova-1811 | Ovis aries | 95.85 | 0.0 | 1232 |
| LLPS-Bot-a175 | Bos taurus | 95.71 | 0.0 | 1222 |
| LLPS-Loa-a167 | Loxodonta africana | 95.42 | 0.0 | 1226 |
| LLPS-Fud-a167 | Fukomys damarensis | 94.62 | 0.0 | 1216 |
| LLPS-Myl-a165 | Myotis lucifugus | 93.85 | 0.0 | 1205 |
| LLPS-Mea-a159 | Mesocricetus auratus | 92.67 | 0.0 | 1194 |
| LLPS-Eqc-2171 | Equus caballus | 91.98 | 0.0 | 1239 |
| LLPS-Mum-3489 | Mus musculus | 91.6 | 0.0 | 1176 |
| LLPS-Ran-3375 | Rattus norvegicus | 90.38 | 0.0 | 1168 |
| LLPS-Mod-a163 | Monodelphis domestica | 87.2 | 0.0 | 1101 |
| LLPS-Cap-2189 | Cavia porcellus | 84.82 | 0.0 | 1043 |
| LLPS-Pes-a153 | Pelodiscus sinensis | 81.5 | 0.0 | 1028 |
| LLPS-Ora-a155 | Ornithorhynchus anatinus | 81.31 | 0.0 | 834 |
| LLPS-Anp-a153 | Anas platyrhynchos | 79.97 | 0.0 | 1021 |
| LLPS-Gaga-3917 | Gallus gallus | 79.76 | 0.0 | 1025 |
| LLPS-Fia-a157 | Ficedula albicollis | 79.73 | 0.0 | 1018 |
| LLPS-Tag-a153 | Taeniopygia guttata | 78.16 | 0.0 | 952 |
| LLPS-Anc-a162 | Anolis carolinensis | 77.26 | 0.0 | 984 |
| LLPS-Cas-0583 | Carlito syrichta | 73.74 | 4e-137 | 427 |
| LLPS-Dio-3154 | Dipodomys ordii | 73.02 | 1e-121 | 382 |
| LLPS-Cii-1858 | Ciona intestinalis | 72.91 | 8e-119 | 378 |
| LLPS-Meg-1627 | Meleagris gallopavo | 71.64 | 0.0 | 822 |
| LLPS-Lac-a157 | Latimeria chalumnae | 69.25 | 0.0 | 873 |
| LLPS-Leo-a148 | Lepisosteus oculatus | 68.5 | 0.0 | 826 |
| LLPS-Xet-a153 | Xenopus tropicalis | 67.37 | 0.0 | 822 |
| LLPS-Orn-a151 | Oreochromis niloticus | 66.67 | 0.0 | 790 |
| LLPS-Scf-a148 | Scleropages formosus | 65.4 | 0.0 | 738 |
| LLPS-Icp-a156 | Ictalurus punctatus | 65.22 | 0.0 | 801 |
| LLPS-Dar-a150 | Danio rerio | 65.13 | 0.0 | 785 |
| LLPS-Ten-a148 | Tetraodon nigroviridis | 63.61 | 0.0 | 776 |
| LLPS-Pof-a152 | Poecilia formosa | 62.96 | 0.0 | 795 |
| LLPS-Xim-a157 | Xiphophorus maculatus | 62.81 | 0.0 | 801 |
| LLPS-Tar-3964 | Takifugu rubripes | 62.7 | 0.0 | 778 |
| LLPS-Asm-a145 | Astyanax mexicanus | 62.46 | 0.0 | 774 |
| LLPS-Scm-a142 | Scophthalmus maximus | 62.09 | 0.0 | 783 |
| LLPS-Sem-2287 | Selaginella moellendorffii | 61.82 | 7e-67 | 234 |
| LLPS-Orl-a149 | Oryzias latipes | 61.82 | 0.0 | 769 |
| LLPS-Hea-1872 | Helianthus annuus | 61.35 | 3e-67 | 238 |
| LLPS-Asn-0815 | Aspergillus nidulans | 60.95 | 8e-62 | 224 |
| LLPS-Bro-a040 | Brassica oleracea | 60.74 | 9e-67 | 236 |
| LLPS-Brn-a048 | Brassica napus | 60.74 | 9e-67 | 236 |
| LLPS-Glm-2038 | Glycine max | 60.71 | 3e-69 | 243 |
| LLPS-Chr-a061 | Chlamydomonas reinhardtii | 60.62 | 4e-62 | 226 |
| LLPS-Nia-a037 | Nicotiana attenuata | 60.61 | 1e-68 | 241 |
| LLPS-Amt-a029 | Amborella trichopoda | 60.61 | 5e-67 | 238 |
| LLPS-Vir-1460 | Vigna radiata | 60.61 | 2e-69 | 243 |
| LLPS-Dac-1523 | Daucus carota | 60.49 | 2e-66 | 236 |
| LLPS-Orr-a036 | Oryza rufipogon | 60.37 | 1e-65 | 233 |
| LLPS-Orni-a037 | Oryza nivara | 60.37 | 2e-65 | 233 |
| LLPS-Orb-a035 | Oryza barthii | 60.37 | 5e-67 | 235 |
| LLPS-Ori-a039 | Oryza indica | 60.37 | 1e-65 | 233 |
| LLPS-Org-a034 | Oryza glaberrima | 60.37 | 1e-65 | 233 |
| LLPS-Orgl-a035 | Oryza glumaepatula | 60.37 | 1e-65 | 233 |
| LLPS-Brr-a036 | Brassica rapa | 60.12 | 9e-67 | 236 |
| LLPS-Lep-a030 | Leersia perrieri | 60.0 | 1e-65 | 234 |
| LLPS-Phv-0585 | Phaseolus vulgaris | 60.0 | 4e-68 | 240 |
| LLPS-Sot-1508 | Solanum tuberosum | 60.0 | 1e-66 | 236 |
| LLPS-Prp-a031 | Prunus persica | 60.0 | 3e-68 | 241 |
| LLPS-Orm-a026 | Oryza meridionalis | 59.76 | 6e-65 | 232 |
| LLPS-Asc-0290 | Aspergillus clavatus | 59.76 | 9e-61 | 221 |
| LLPS-Gaa-2797 | Gasterosteus aculeatus | 59.61 | 0.0 | 777 |
| LLPS-Pot-1802 | Populus trichocarpa | 59.52 | 1e-68 | 242 |
| LLPS-Cym-1088 | Cyanidioschyzon merolae | 59.51 | 2e-59 | 221 |
| LLPS-Coc-2034 | Corchorus capsularis | 59.39 | 2e-67 | 239 |
| LLPS-Via-1553 | Vigna angularis | 59.39 | 2e-67 | 238 |
| LLPS-Orp-a030 | Oryza punctata | 59.39 | 3e-65 | 233 |
| LLPS-Mua-a037 | Musa acuminata | 59.39 | 3e-66 | 233 |
| LLPS-Viv-2103 | Vitis vinifera | 59.39 | 5e-67 | 237 |
| LLPS-Ast-1409 | Aspergillus terreus | 59.3 | 9e-62 | 223 |
| LLPS-Lem-1158 | Leptosphaeria maculans | 59.3 | 3e-61 | 222 |
| LLPS-Chc-0552 | Chondrus crispus | 59.26 | 2e-56 | 209 |
| LLPS-Asni-0788 | Aspergillus niger | 59.17 | 1e-59 | 218 |
| LLPS-Sob-a040 | Sorghum bicolor | 59.15 | 3e-65 | 233 |
| LLPS-Zem-a036 | Zea mays | 59.15 | 6e-65 | 232 |
| LLPS-Met-1848 | Medicago truncatula | 58.93 | 4e-68 | 240 |
| LLPS-Gas-a009 | Galdieria sulphuraria | 58.9 | 5e-60 | 216 |
| LLPS-Sol-0754 | Solanum lycopersicum | 58.9 | 1e-65 | 233 |
| LLPS-Orbr-a036 | Oryza brachyantha | 58.79 | 7e-65 | 232 |
| LLPS-Thc-2016 | Theobroma cacao | 58.79 | 3e-67 | 238 |
| LLPS-Sei-a033 | Setaria italica | 58.79 | 5e-65 | 233 |
| LLPS-Art-1522 | Arabidopsis thaliana | 58.79 | 3e-66 | 234 |
| LLPS-Sah-a162 | Sarcophilus harrisii | 58.59 | 0.0 | 716 |
| LLPS-Coo-a008 | Colletotrichum orbiculare | 58.56 | 3e-63 | 226 |
| LLPS-Pytr-0143 | Pyrenophora triticirepentis | 58.43 | 5e-62 | 225 |
| LLPS-Pyt-a011 | Pyrenophora teres | 58.43 | 4e-62 | 225 |
| LLPS-Osl-a028 | Ostreococcus lucimarinus | 58.18 | 5e-61 | 217 |
| LLPS-Gag-a007 | Gaeumannomyces graminis | 58.01 | 3e-62 | 223 |
| LLPS-Aso-0046 | Aspergillus oryzae | 57.92 | 3e-63 | 226 |
| LLPS-Asf-0259 | Aspergillus flavus | 57.92 | 3e-63 | 228 |
| LLPS-Trv-a008 | Trichoderma virens | 57.84 | 8e-64 | 229 |
| LLPS-Trr-1515 | Trichoderma reesei | 57.84 | 2e-63 | 226 |
| LLPS-Asfu-0019 | Aspergillus fumigatus | 57.78 | 7e-61 | 221 |
| LLPS-Nef-0764 | Neosartorya fischeri | 57.78 | 7e-61 | 221 |
| LLPS-Cus-a028 | Cucumis sativus | 57.74 | 3e-64 | 230 |
| LLPS-Abg-a015 | Absidia glauca | 57.32 | 2e-55 | 207 |
| LLPS-Scs-1073 | Sclerotinia sclerotiorum | 57.3 | 1e-63 | 229 |
| LLPS-Tra-a048 | Triticum aestivum | 57.23 | 6e-64 | 229 |
| LLPS-Phn-a009 | Phaeosphaeria nodorum | 56.97 | 2e-55 | 204 |
| LLPS-Hov-a031 | Hordeum vulgare | 56.89 | 5e-62 | 224 |
| LLPS-Drm-a049 | Drosophila melanogaster | 56.78 | 3e-70 | 254 |
| LLPS-Mel-0834 | Melampsora laricipopulina | 55.72 | 4e-62 | 229 |
| LLPS-Zyt-0421 | Zymoseptoria tritici | 55.68 | 6e-60 | 218 |
| LLPS-Crn-1464 | Cryptococcus neoformans | 54.23 | 4e-59 | 217 |
| LLPS-Blg-1448 | Blumeria graminis | 54.15 | 2e-61 | 223 |
| LLPS-Scj-a005 | Schizosaccharomyces japonicus | 53.99 | 3e-53 | 198 |
| LLPS-Brd-a035 | Brachypodium distachyon | 53.98 | 1e-60 | 221 |
| LLPS-Tru-a025 | Triticum urartu | 53.98 | 2e-61 | 222 |
| LLPS-Mae-1555 | Manihot esculenta | 53.92 | 1e-67 | 238 |
| LLPS-Yal-a010 | Yarrowia lipolytica | 53.77 | 2e-60 | 218 |
| LLPS-Nec-a011 | Neurospora crassa | 53.4 | 4e-63 | 227 |
| LLPS-Ved-0078 | Verticillium dahliae | 53.33 | 2e-64 | 229 |
| LLPS-Ors-a039 | Oryza sativa | 53.17 | 6e-58 | 209 |
| LLPS-Beb-a007 | Beauveria bassiana | 52.88 | 2e-63 | 228 |
| LLPS-Cogr-1601 | Colletotrichum graminicola | 52.86 | 2e-63 | 227 |
| LLPS-Cog-0792 | Colletotrichum gloeosporioides | 52.86 | 4e-64 | 228 |
| LLPS-Arl-2545 | Arabidopsis lyrata | 52.45 | 4e-67 | 238 |
| LLPS-Fuo-1531 | Fusarium oxysporum | 52.38 | 2e-63 | 227 |
| LLPS-Fuv-0382 | Fusarium verticillioides | 52.38 | 2e-63 | 227 |
| LLPS-Cae-1614 | Caenorhabditis elegans | 52.37 | 4e-83 | 290 |
| LLPS-Spr-0503 | Sporisorium reilianum | 51.9 | 6e-59 | 219 |
| LLPS-Usm-1548 | Ustilago maydis | 51.9 | 4e-59 | 220 |
| LLPS-Tum-0290 | Tuber melanosporum | 51.6 | 6e-60 | 219 |
| LLPS-Scc-a005 | Schizosaccharomyces cryophilus | 50.57 | 2e-52 | 196 |
| LLPS-Scp-a006 | Schizosaccharomyces pombe | 50.28 | 1e-51 | 194 |