LLPS-Hos-2003
CSNK2A2

▼ OVERVIEW

Status: Reviewed
Protein Name: Casein kinase II subunit alpha'
Gene Name: CSNK2A2, CK2A2
Ensembl Gene: ENSG00000070770.8
Ensembl Protein: ENSP00000456606.2
Organism: Homo sapiens
Taxa ID: 9606
LLPS Type: Client
PDB: 5M4U (A) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Nucleolus
"...SILAC-based approaches identified 351 proteins with 24 proteins showing at least a 2-fold change after infection."
HeLa cells19812395
Nucleolus
"...As a first step in elucidating these mechanisms we have carried out a proteomic analysis to draw up a list of proteins present within nucleoli of HeLa cells. This analysis allowed the identification of 213 different nucleolar proteins."
HeLa cells12429849
Nucleolus
"...The data set from all our nucleolar mass spectrometry analyses defines an updated group of 692 proteins that reproducibly copurify with human nucleoli."
HeLa cells15635413
Centrosome/Spindle pole body
"...MiCroKiTS 4.0: a database of midbody, centrosome, kinetochore, telomere and spindle."
N/A12568341" target="_blank">12568341, 12568341" target="_blank">12568341
Postsynaptic density
"...We report the first direct comparison of the proteome of triplicate isolates of mouse and human cortical postsynaptic densities. The mouse postsynaptic density comprised 1556 proteins and the human one 1461."
Human cortex23071613

▼ FUNCTION

Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV.

▼ SEQUENCE

Protein Sequence (FASTA)
1     MPGPAAGSRA  RVYAEVNSLR  SREYWDYEAH  VPSWGNQDDY  QLVRKLGRGK  YSEVFEAINI  60
61    TNNERVVVKI  LKPVKKKKIK  REVKILENLR  GGTNIIKLID  TVKDPVSKTP  ALVFEYINNT  120
121   DFKQLYQILT  DFDIRFYMYE  LLKALDYCHS  KGIMHRDVKP  HNVMIDHQQK  KLRLIDWGLA  180
181   EFYHPAQEYN  VRVASRYFKG  PELLVDYQMY  DYSLDMWSLG  CMLASMIFRR  EPFFHGQDNY  240
241   DQLVRIAKVL  GTEELYGYLK  KYHIDLDPHF  NDILGQHSRK  RWENFIHSEN  RHLVSPEALD  300
301   LLDKLLRYDH  QQRLTAKEAM  EHPYFYPVVK  EQSQPCADNA  VLSSGLTAAR  350
Nucleotide CDS Sequence (FASTA)
1     ATGCCCGGCC  CGGCCGCGGG  CAGCAGGGCC  CGGGTCTACG  CCGAGGTGAA  CAGTCTGAGG  60
61    AGCCGCGAGT  ACTGGGACTA  CGAGGCTCAC  GTCCCGAGCT  GGGGTAATCA  AGATGATTAC  120
121   CAACTGGTTC  GAAAACTTGG  TCGGGGAAAA  TATAGTGAAG  TATTTGAGGC  CATTAATATC  180
181   ACCAACAATG  AGAGAGTGGT  TGTAAAAATC  CTGAAGCCAG  TGAAGAAAAA  GAAGATAAAA  240
241   CGAGAGGTTA  AGATTCTGGA  GAACCTTCGT  GGTGGAACAA  ATATCATTAA  GCTGATTGAC  300
301   ACTGTAAAGG  ACCCCGTGTC  AAAGACACCA  GCTTTGGTAT  TTGAATATAT  CAATAATACA  360
361   GATTTTAAGC  AACTCTACCA  GATCCTGACA  GACTTTGATA  TCCGGTTTTA  TATGTATGAA  420
421   CTACTTAAAG  CTCTGGATTA  CTGCCACAGC  AAGGGAATCA  TGCACAGGGA  TGTGAAACCT  480
481   CACAATGTCA  TGATAGATCA  CCAACAGAAA  AAGCTGCGAC  TGATAGATTG  GGGTCTGGCA  540
541   GAATTCTATC  ATCCTGCTCA  GGAGTACAAT  GTTCGTGTAG  CCTCAAGGTA  CTTCAAGGGA  600
601   CCAGAGCTCC  TCGTGGACTA  TCAGATGTAT  GATTATAGCT  TGGACATGTG  GAGTTTGGGC  660
661   TGTATGTTAG  CAAGCATGAT  CTTTCGAAGG  GAACCATTCT  TCCATGGACA  GGACAACTAT  720
721   GACCAGCTTG  TTCGCATTGC  CAAGGTTCTG  GGTACAGAAG  AACTGTATGG  GTATCTGAAG  780
781   AAGTATCACA  TAGACCTAGA  TCCACACTTC  AACGATATCC  TGGGACAACA  TTCACGGAAA  840
841   CGCTGGGAAA  ACTTTATCCA  TAGTGAGAAC  AGACACCTTG  TCAGCCCTGA  GGCCCTAGAT  900
901   CTTCTGGACA  AACTTCTGCG  ATACGACCAT  CAACAGAGAC  TGACTGCCAA  AGAGGCCATG  960
961   GAGCACCCAT  ACTTCTACCC  TGTGGTGAAG  GAGCAGTCCC  AGCCTTGTGC  AGACAATGCT  1020
1021  GTGCTTTCCA  GTGGTCTCAC  GGCAGCACGA  TGA  1053

▼ KEYWORD

ID
Family
3D-structure
Acetylation
Apoptosis
ATP-binding
Cell cycle
Complete proteome
Kinase
Nucleotide-binding
Phosphoprotein
Polymorphism
Reference proteome
Serine/threonine-protein kinase
Transcription
Transcription regulation
Transferase
Wnt signaling pathway

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Acrosomal vesicle
Cellular Component
Chromatin
Cellular Component
Cytosol
Cellular Component
Nucleoplasm
Cellular Component
Nucleus
Cellular Component
Plasma membrane
Molecular Function
ATP binding
Molecular Function
Protein N-terminus binding
Molecular Function
Protein serine/threonine kinase activity
Biological Process
Apoptotic process
Biological Process
Cell cycle
Biological Process
Cerebral cortex development
Biological Process
Liver regeneration
Biological Process
Macroautophagy
Biological Process
Negative regulation of apoptotic signaling pathway
Biological Process
Negative regulation of ubiquitin-dependent protein catabolic process
Biological Process
Peptidyl-serine phosphorylation
Biological Process
Phosphatidylcholine biosynthetic process
Biological Process
Positive regulation of protein targeting to mitochondrion
Biological Process
Protein folding
Biological Process
Regulation of autophagy of mitochondrion
Biological Process
Regulation of chromosome separation
Biological Process
Regulation of signal transduction by p53 class mediator
Biological Process
Spermatogenesis
Biological Process
Wnt signaling pathway

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Pat-1325Pan troglodytes100.00.0 676
LLPS-Mam-1673Macaca mulatta100.00.0 676
LLPS-Poa-4122Pongo abelii100.00.0 629
LLPS-Cea-2686Cercocebus atys100.00.0 676
LLPS-Chs-1479Chlorocebus sabaeus100.00.0 676
LLPS-Maf-3009Macaca fascicularis100.00.0 676
LLPS-Gog-3489Gorilla gorilla100.00.0 602
LLPS-Paa-3534Papio anubis100.00.0 676
LLPS-Rhb-0500Rhinopithecus bieti99.710.0 674
LLPS-Aon-1280Aotus nancymaae99.710.0 675
LLPS-Caj-0640Callithrix jacchus99.710.0 675
LLPS-Fec-0230Felis catus99.430.0 673
LLPS-Bot-1166Bos taurus99.430.0 673
LLPS-Pap-3677Pan paniscus99.370.0 603
LLPS-Caf-3149Canis familiaris99.370.0 602
LLPS-Mal-2012Mandrillus leucophaeus99.370.0 603
LLPS-Myl-1837Myotis lucifugus99.240.0 550
LLPS-Eqc-1526Equus caballus99.140.0 672
LLPS-Sus-3797Sus scrofa99.140.0 672
LLPS-Fud-0197Fukomys damarensis99.050.0 597
LLPS-Ova-1381Ovis aries99.050.0 596
LLPS-Loa-2184Loxodonta africana98.860.0 665
LLPS-Mum-2912Mus musculus98.860.0 671
LLPS-Urm-3746Ursus maritimus98.730.0 593
LLPS-Ict-0305Ictidomys tridecemlineatus98.730.0 598
LLPS-Otg-2155Otolemur garnettii98.570.0 668
LLPS-Cap-2411Cavia porcellus98.570.0 664
LLPS-Ran-1608Rattus norvegicus98.570.0 669
LLPS-Man-2390Macaca nemestrina98.550.0 650
LLPS-Ora-0707Ornithorhynchus anatinus98.290.0 665
LLPS-Mod-3329Monodelphis domestica97.780.0 589
LLPS-Dio-2198Dipodomys ordii97.150.0 651
LLPS-Sah-2612Sarcophilus harrisii96.840.0 587
LLPS-Meg-0520Meleagris gallopavo96.720.0 564
LLPS-Pes-1105Pelodiscus sinensis96.550.0 590
LLPS-Anp-1225Anas platyrhynchos96.50.0 574
LLPS-Mup-0822Mustela putorius furo96.320.0 596
LLPS-Tag-2744Taeniopygia guttata96.290.0 649
LLPS-Fia-0921Ficedula albicollis95.70.0 647
LLPS-Anc-0998Anolis carolinensis94.950.0 571
LLPS-Nol-3497Nomascus leucogenys94.280.0 590
LLPS-Cas-3278Carlito syrichta94.070.0 628
LLPS-Mea-0775Mesocricetus auratus91.930.0 597
LLPS-Xet-1059Xenopus tropicalis90.570.0 617
LLPS-Gaga-1332Gallus gallus89.820.0 564
LLPS-Lac-0592Latimeria chalumnae88.920.0 579
LLPS-Ten-1432Tetraodon nigroviridis88.412e-167 476
LLPS-Pof-0699Poecilia formosa86.860.0 600
LLPS-Leo-0917Lepisosteus oculatus86.860.0 603
LLPS-Asm-2210Astyanax mexicanus86.860.0 597
LLPS-Xim-0538Xiphophorus maculatus86.570.0 598
LLPS-Orn-1336Oreochromis niloticus86.290.0 596
LLPS-Orl-3897Oryzias latipes85.956e-174 496
LLPS-Dar-0131Danio rerio85.710.0 593
LLPS-Scf-1641Scleropages formosus85.710.0 592
LLPS-Tar-0385Takifugu rubripes85.190.0 587
LLPS-Icp-1103Ictalurus punctatus85.140.0 588
LLPS-Gaa-3070Gasterosteus aculeatus84.730.0 553
LLPS-Scm-1959Scophthalmus maximus84.430.0 551
LLPS-Drm-0787Drosophila melanogaster81.710.0 521
LLPS-Orc-1395Oryctolagus cuniculus81.410.0 555
LLPS-Tut-0040Tursiops truncatus81.410.0 555
LLPS-Aim-2166Ailuropoda melanoleuca81.410.0 555
LLPS-Cis-0058Ciona savignyi79.360.0 533
LLPS-Cii-1782Ciona intestinalis79.360.0 536
LLPS-Cae-1210Caenorhabditis elegans79.150.0 513
LLPS-Php-1462Physcomitrella patens75.392e-166 475
LLPS-Tru-0835Triticum urartu75.086e-160 458
LLPS-Hov-1582Hordeum vulgare75.087e-160 458
LLPS-Lep-1435Leersia perrieri75.01e-134 395
LLPS-Orr-1194Oryza rufipogon74.771e-161 465
LLPS-Org-0498Oryza glaberrima74.478e-166 476
LLPS-Orb-0800Oryza barthii74.478e-166 476
LLPS-Orni-1373Oryza nivara74.478e-166 476
LLPS-Brd-1605Brachypodium distachyon74.453e-163 467
LLPS-Orm-0182Oryza meridionalis74.175e-150 432
LLPS-Sob-0016Sorghum bicolor74.144e-163 469
LLPS-Nia-1631Nicotiana attenuata73.835e-163 466
LLPS-Cus-0918Cucumis sativus73.832e-162 465
LLPS-Viv-0967Vitis vinifera73.621e-162 465
LLPS-Orbr-0522Oryza brachyantha73.583e-160 459
LLPS-Sol-0645Solanum lycopersicum73.574e-158 454
LLPS-Ors-1858Oryza sativa73.525e-160 459
LLPS-Coc-0429Corchorus capsularis73.522e-163 468
LLPS-Zem-0012Zea mays73.528e-162 465
LLPS-Thc-1510Theobroma cacao73.522e-162 465
LLPS-Mua-1774Musa acuminata73.522e-163 467
LLPS-Amt-1286Amborella trichopoda73.212e-162 465
LLPS-Orp-1210Oryza punctata73.113e-164 469
LLPS-Orgl-1419Oryza glumaepatula73.111e-164 471
LLPS-Mae-2679Manihot esculenta73.091e-163 471
LLPS-Pot-0533Populus trichocarpa73.012e-164 470
LLPS-Gor-0816Gossypium raimondii73.019e-165 473
LLPS-Tra-1776Triticum aestivum72.954e-162 466
LLPS-Hea-0258Helianthus annuus72.92e-160 463
LLPS-Via-1906Vigna angularis72.73e-164 469
LLPS-Sei-0451Setaria italica72.71e-162 470
LLPS-Met-2124Medicago truncatula72.73e-163 469
LLPS-Osl-1188Ostreococcus lucimarinus72.592e-161 462
LLPS-Bro-0725Brassica oleracea72.563e-161 463
LLPS-Glm-1965Glycine max72.567e-164 471
LLPS-Phv-0540Phaseolus vulgaris72.392e-162 465
LLPS-Brn-0445Brassica napus72.399e-162 463
LLPS-Prp-1960Prunus persica72.273e-159 457
LLPS-Art-1753Arabidopsis thaliana71.831e-160 463
LLPS-Brr-1643Brassica rapa71.62e-162 467
LLPS-Arl-2608Arabidopsis lyrata71.122e-162 469
LLPS-Vir-1975Vigna radiata70.984e-132 385
LLPS-Ori-0592Oryza indica70.914e-155 448
LLPS-Abg-1758Absidia glauca70.343e-157 452
LLPS-Beb-0341Beauveria bassiana69.911e-149 433
LLPS-Usm-1430Ustilago maydis69.858e-155 446
LLPS-Crn-1332Cryptococcus neoformans69.788e-153 441
LLPS-Mel-0072Melampsora laricipopulina69.516e-156 448
LLPS-Dac-1621Daucus carota69.397e-155 448
LLPS-Fuo-0123Fusarium oxysporum69.284e-151 436
LLPS-Fuv-1227Fusarium verticillioides69.284e-151 436
LLPS-Fus-0695Fusarium solani68.972e-149 432
LLPS-Coo-0347Colletotrichum orbiculare68.541e-146 425
LLPS-Ved-1000Verticillium dahliae68.342e-148 430
LLPS-Miv-1147Microbotryum violaceum68.22e-155 447
LLPS-Scj-0450Schizosaccharomyces japonicus67.925e-147 426
LLPS-Pug-0648Puccinia graminis67.861e-155 451
LLPS-Zyt-1335Zymoseptoria tritici67.713e-147 426
LLPS-Cogr-1071Colletotrichum graminicola66.674e-145 422
LLPS-Put-0819Puccinia triticina66.474e-156 449
LLPS-Cog-0524Colletotrichum gloeosporioides66.469e-140 407
LLPS-Blg-1508Blumeria graminis65.888e-152 438
LLPS-Gas-1061Galdieria sulphuraria62.771e-139 407
LLPS-Kop-1162Komagataella pastoris60.981e-130 384
LLPS-Yal-0821Yarrowia lipolytica59.51e-128 379
LLPS-Asg-1090Ashbya gossypii56.111e-122 365