LLPS-Hos-0981
AP2B1

▼ OVERVIEW

Status: Reviewed
Protein Name: AP-2 complex subunit beta; AP105B; Adaptor protein complex AP-2 subunit beta; Adaptor-related protein complex 2 subunit beta; Beta-2-adaptin; Beta-adaptin; Clathrin assembly protein complex 2 beta large chain; Plasma membrane adaptor HA2/AP2 adaptin beta subunit
Gene Name: AP2B1, ADTB2, CLAPB1
Ensembl Gene: ENSG00000006125.17
Ensembl Protein: ENSP00000478115.1
Organism: Homo sapiens
Taxa ID: 9606
LLPS Type: LLPS regulator
PDB: 2JKT (A, B, M, S, P) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Nucleolus
"...Thus, we analyzed the orthologous groups of the remaining 2316 identified proteins to the human nucleolar proteins. We detected 361 orthologues in NoPDB from the 2316 A. thaliana proteins."
N/A26980300
Postsynaptic density
"...We report the first direct comparison of the proteome of triplicate isolates of mouse and human cortical postsynaptic densities. The mouse postsynaptic density comprised 1556 proteins and the human one 1461."
Human cortex23071613

▼ FUNCTION

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 beta subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins; at least some clathrin-associated sorting proteins (CLASPs) are recognized by their [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif. The AP-2 beta subunit binds to clathrin heavy chain, promoting clathrin lattice assembly; clathrin displaces at least some CLASPs from AP2B1 which probably then can be positioned for further coat assembly.

▼ SEQUENCE

Protein Sequence (FASTA)
1     MTDSKYFTTN  KKGEIFELKA  ELNNEKKEKR  KEAVKKVIAA  MTVGKDVSSL  FPDVVNCMQT  60
61    DNLELKKLVY  LYLMNYAKSQ  PDMAIMAVNS  FVKDCEDPNP  LIRALAVRTM  GCIRVDKITE  120
121   YLCEPLRKCL  KDEDPYVRKT  AAVCVAKLHD  INAQMVEDQG  FLDSLRDLIA  DSNPMVVANA  180
181   VAALSEISES  HPNSNLLDLN  PQNINKLLTA  LNECTEWGQI  FILDCLSNYN  PKDDREAQSI  240
241   CERVTPRLSH  ANSAVVLSAV  KVLMKFLELL  PKDSDYYNML  LKKLAPPLVT  LLSGEPEVQY  300
301   VALRNINLIV  QKRPEILKQE  IKVFFVKYND  PIYVKLEKLD  IMIRLASQAN  IAQVLAELKE  360
361   YATEVDVDFV  RKAVRAIGRC  AIKVEQSAER  CVSTLLDLIQ  TKVNYVVQEA  IVVIRDIFRK  420
421   YPNKYESIIA  TLCENLDSLD  EPDARAAMIW  IVGEYAERID  NADELLESFL  EGFHDESTQV  480
481   QLTLLTAIVK  LFLKKPSETQ  ELVQQVLSLA  TQDSDNPDLR  DRGYIYWRLL  STDPVTAKEV  540
541   VLSEKPLISE  ETDLIEPTLL  DELICHIGSL  ASVYHKPPNA  FVEGSHGIHR  KHLPIHHGST  600
601   DAGDSPVGTT  TATNLEQPQV  IPSQGDLLGD  LLNLDLGPPV  NVPQVSSMQM  GAVDLLGGGL  660
661   DSLLGSDLGG  GIGGSPAVGQ  SFIPSSVPAT  FAPSPTPAVV  SSGLNDLFEL  STGIGMAPGG  720
721   YVAPKAVWLP  AVKAKGLEIS  GTFTHRQGHI  YMEMNFTNKA  LQHMTDFAIQ  FNKNSFGVIP  780
781   STPLAIHTPL  MPNQSIDVSL  PLNTLGPVMK  MEPLNNLQVA  VKNNIDVFYF  SCLIPLNVLF  840
841   VEDGKMERQV  FLATWKDIPN  ENELQFQIKE  CHLNADTVSS  KLQNNNVYTI  AKRNVEGQDM  900
901   LYQSLKLTNG  IWILAELRIQ  PGNPNYTLSL  KCRAPEVSQY  IYQVYDSILK  N  951
Nucleotide CDS Sequence (FASTA)
1     ATGACTGACT  CCAAGTATTT  CACAACCAAT  AAAAAAGGAG  AAATATTTGA  ACTAAAAGCT  60
61    GAACTCAACA  ATGAAAAGAA  AGAAAAGAGA  AAGGAGGCTG  TGAAGAAAGT  GATTGCTGCT  120
121   ATGACCGTGG  GGAAGGATGT  TAGGTGCTGC  TCTTCTGAGG  TGAATTTTTG  TTATTTTTCT  180
181   TTTGTTTGTT  AG  192

▼ KEYWORD

ID
Family
3D-structure
Acetylation
Alternative splicing
Cell membrane
Coated pit
Complete proteome
Endocytosis
Membrane
Phosphoprotein
Protein transport
Reference proteome
Transport

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
AP-2 adaptor complex
Cellular Component
Clathrin-coated endocytic vesicle
Cellular Component
Clathrin-coated endocytic vesicle membrane
Cellular Component
Cytosol
Cellular Component
Endocytic vesicle membrane
Cellular Component
Endolysosome membrane
Cellular Component
Glutamatergic synapse
Cellular Component
Membrane
Cellular Component
Plasma membrane
Cellular Component
Postsynapse
Cellular Component
Presynapse
Molecular Function
Cargo receptor activity
Molecular Function
Clathrin binding
Molecular Function
Protein-containing complex binding
Molecular Function
Signal sequence binding
Biological Process
Antigen processing and presentation of exogenous peptide antigen via MHC class II
Biological Process
Aorta development
Biological Process
Clathrin coat assembly
Biological Process
Clathrin-dependent endocytosis
Biological Process
Coronary vasculature development
Biological Process
Ephrin receptor signaling pathway
Biological Process
Low-density lipoprotein particle clearance
Biological Process
Low-density lipoprotein particle receptor catabolic process
Biological Process
Membrane organization
Biological Process
Negative regulation of neuron death
Biological Process
Positive regulation of endocytosis
Biological Process
Positive regulation of protein localization to membrane
Biological Process
Postsynaptic neurotransmitter receptor internalization
Biological Process
Regulation of defense response to virus by virus
Biological Process
Synaptic vesicle endocytosis
Biological Process
Ventricular septum development
Biological Process
Vesicle-mediated transport
Biological Process
Wnt signaling pathway, planar cell polarity pathway

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Loa-0999Loxodonta africana100.00.01813
LLPS-Pat-3273Pan troglodytes100.00.01813
LLPS-Urm-0942Ursus maritimus100.00.01813
LLPS-Pap-2032Pan paniscus100.00.01813
LLPS-Fec-2636Felis catus100.00.01813
LLPS-Rhb-2422Rhinopithecus bieti100.00.01813
LLPS-Caf-1806Canis familiaris100.00.01813
LLPS-Eqc-0173Equus caballus100.00.01813
LLPS-Ict-2734Ictidomys tridecemlineatus100.00.01813
LLPS-Mea-3976Mesocricetus auratus100.00.01813
LLPS-Maf-3770Macaca fascicularis100.00.01813
LLPS-Chs-2633Chlorocebus sabaeus100.00.01813
LLPS-Caj-1159Callithrix jacchus100.00.01813
LLPS-Ran-2261Rattus norvegicus100.00.01813
LLPS-Orc-2748Oryctolagus cuniculus99.890.01811
LLPS-Poa-0221Pongo abelii99.890.01809
LLPS-Cap-0869Cavia porcellus99.890.01811
LLPS-Nol-1496Nomascus leucogenys99.890.01811
LLPS-Sus-0897Sus scrofa99.890.01810
LLPS-Mum-4070Mus musculus99.890.01811
LLPS-Fud-2933Fukomys damarensis99.890.01811
LLPS-Gog-3707Gorilla gorilla99.890.01811
LLPS-Dio-3384Dipodomys ordii99.890.01810
LLPS-Bot-2608Bos taurus99.890.01811
LLPS-Tut-0155Tursiops truncatus99.890.01811
LLPS-Mup-3688Mustela putorius furo99.890.01656
LLPS-Ova-3882Ovis aries99.790.01810
LLPS-Aon-1926Aotus nancymaae99.670.01761
LLPS-Mod-0419Monodelphis domestica99.370.01803
LLPS-Aim-3080Ailuropoda melanoleuca99.370.01799
LLPS-Fia-2782Ficedula albicollis99.050.01800
LLPS-Gaga-1679Gallus gallus98.950.01798
LLPS-Paa-3361Papio anubis98.950.01788
LLPS-Man-4320Macaca nemestrina98.850.01795
LLPS-Cea-3540Cercocebus atys98.850.01795
LLPS-Anp-0033Anas platyrhynchos98.840.01793
LLPS-Meg-1364Meleagris gallopavo98.840.01793
LLPS-Myl-1478Myotis lucifugus97.90.01789
LLPS-Cas-3851Carlito syrichta97.870.01731
LLPS-Tag-2461Taeniopygia guttata97.480.01769
LLPS-Xet-3399Xenopus tropicalis96.650.01753
LLPS-Otg-0611Otolemur garnettii96.110.01721
LLPS-Mam-2818Macaca mulatta95.10.01707
LLPS-Mal-4202Mandrillus leucophaeus94.90.01694
LLPS-Asm-2397Astyanax mexicanus94.850.01730
LLPS-Icp-0045Ictalurus punctatus94.740.01730
LLPS-Scf-1058Scleropages formosus94.640.01725
LLPS-Dar-4095Danio rerio94.530.01722
LLPS-Orl-2316Oryzias latipes93.910.01716
LLPS-Xim-0364Xiphophorus maculatus93.910.01702
LLPS-Ten-2644Tetraodon nigroviridis93.810.01697
LLPS-Lac-3676Latimeria chalumnae93.70.01689
LLPS-Orn-3585Oreochromis niloticus93.70.01693
LLPS-Tar-1320Takifugu rubripes93.60.01692
LLPS-Pof-3918Poecilia formosa93.360.01528
LLPS-Scm-2447Scophthalmus maximus93.180.01689
LLPS-Gaa-2551Gasterosteus aculeatus93.170.01707
LLPS-Leo-1103Lepisosteus oculatus91.90.01705
LLPS-Drm-0572Drosophila melanogaster82.880.01019
LLPS-Anc-2679Anolis carolinensis82.080.01488
LLPS-Sah-2980Sarcophilus harrisii79.010.01329
LLPS-Cae-0043Caenorhabditis elegans76.190.0 971
LLPS-Brr-2133Brassica rapa74.70.0 825
LLPS-Bro-1131Brassica oleracea74.70.0 825
LLPS-Brn-0991Brassica napus74.70.0 825
LLPS-Art-2902Arabidopsis thaliana74.530.0 836
LLPS-Coc-1905Corchorus capsularis74.350.0 833
LLPS-Viv-2339Vitis vinifera74.180.0 853
LLPS-Gor-1196Gossypium raimondii74.010.0 832
LLPS-Thc-0002Theobroma cacao74.010.0 832
LLPS-Via-2229Vigna angularis74.010.0 833
LLPS-Pot-0360Populus trichocarpa73.840.0 851
LLPS-Lep-2118Leersia perrieri73.670.0 811
LLPS-Sei-0849Setaria italica73.670.0 809
LLPS-Phv-0692Phaseolus vulgaris73.490.0 810
LLPS-Tra-2515Triticum aestivum73.490.0 807
LLPS-Glm-2601Glycine max73.340.0 834
LLPS-Pes-2209Pelodiscus sinensis73.180.01276
LLPS-Dac-1370Daucus carota73.180.0 758
LLPS-Hea-1612Helianthus annuus72.670.0 827
LLPS-Zem-2224Zea mays72.60.0 790
LLPS-Cii-2320Ciona intestinalis72.340.01082
LLPS-Nia-2414Nicotiana attenuata72.160.0 839
LLPS-Prp-1955Prunus persica72.110.0 838
LLPS-Sol-1918Solanum lycopersicum72.040.0 838
LLPS-Sot-0978Solanum tuberosum72.040.0 839
LLPS-Amt-1237Amborella trichopoda71.90.0 831
LLPS-Mua-2491Musa acuminata71.450.0 827
LLPS-Mae-2366Manihot esculenta70.990.0 850
LLPS-Cis-1306Ciona savignyi70.690.01050
LLPS-Php-1375Physcomitrella patens70.540.0 808
LLPS-Met-1241Medicago truncatula70.520.0 830
LLPS-Cus-0788Cucumis sativus70.510.0 833
LLPS-Arl-1168Arabidopsis lyrata70.30.0 823
LLPS-Sem-0274Selaginella moellendorffii68.580.0 811
LLPS-Tru-1978Triticum urartu68.210.0 780
LLPS-Abg-0967Absidia glauca66.270.0 755
LLPS-Asc-1477Aspergillus clavatus61.30.0 707
LLPS-Asfu-0725Aspergillus fumigatus60.790.0 703
LLPS-Asf-0633Aspergillus flavus60.740.0 650
LLPS-Nef-1068Neosartorya fischeri60.620.0 701
LLPS-Put-0844Puccinia triticina60.540.0 566
LLPS-Miv-0857Microbotryum violaceum60.470.0 708
LLPS-Ast-0982Aspergillus terreus60.450.0 692
LLPS-Aso-0666Aspergillus oryzae60.450.0 703
LLPS-Asni-1467Aspergillus niger60.270.0 703
LLPS-Tum-1127Tuber melanosporum60.260.0 724
LLPS-Cog-0175Colletotrichum gloeosporioides60.250.0 656
LLPS-Nec-1052Neurospora crassa59.770.0 703
LLPS-Pug-1415Puccinia graminis59.60.0 724
LLPS-Mel-0408Melampsora laricipopulina59.50.0 712
LLPS-Fuo-0354Fusarium oxysporum59.040.0 720
LLPS-Ved-1073Verticillium dahliae59.040.0 665
LLPS-Fuv-0720Fusarium verticillioides59.040.0 721
LLPS-Blg-1103Blumeria graminis58.980.0 723
LLPS-Usm-1105Ustilago maydis58.880.0 700
LLPS-Gag-0119Gaeumannomyces graminis58.710.0 695
LLPS-Coo-0015Colletotrichum orbiculare58.710.0 681
LLPS-Asn-1304Aspergillus nidulans58.540.0 686
LLPS-Crn-1291Cryptococcus neoformans58.520.0 705
LLPS-Gas-1014Galdieria sulphuraria58.460.0 685
LLPS-Mao-0624Magnaporthe oryzae58.40.0 686
LLPS-Fus-0327Fusarium solani58.290.0 719
LLPS-Pytr-0494Pyrenophora triticirepentis58.190.0 684
LLPS-Spr-0715Sporisorium reilianum58.140.0 699
LLPS-Dos-1519Dothistroma septosporum57.450.0 684
LLPS-Zyt-0192Zymoseptoria tritici57.40.0 682
LLPS-Pyt-1043Pyrenophora teres57.170.0 685
LLPS-Beb-0951Beauveria bassiana56.980.0 702
LLPS-Cogr-0229Colletotrichum graminicola56.870.0 662
LLPS-Trv-1625Trichoderma virens56.550.0 652
LLPS-Trr-0942Trichoderma reesei56.380.0 650
LLPS-Lem-0528Leptosphaeria maculans56.380.0 663
LLPS-Vir-1893Vigna radiata56.210.0 943
LLPS-Osl-0191Ostreococcus lucimarinus54.130.0 591
LLPS-Map-0556Magnaporthe poae53.790.0 604
LLPS-Yal-0401Yarrowia lipolytica53.280.0 629
LLPS-Phn-0170Phaeosphaeria nodorum52.262e-157 483
LLPS-Scs-0244Sclerotinia sclerotiorum50.920.0 612
LLPS-Scj-0886Schizosaccharomyces japonicus50.320.0 575
LLPS-Chr-0084Chlamydomonas reinhardtii49.326e-156 488
LLPS-Scc-1007Schizosaccharomyces cryophilus48.825e-175 533
LLPS-Kop-1240Komagataella pastoris48.627e-163 500
LLPS-Chc-1196Chondrus crispus46.683e-171 530
LLPS-Scp-0685Schizosaccharomyces pombe46.180.0 550
LLPS-Asg-0502Ashbya gossypii42.611e-134 427
LLPS-Sac-1597Saccharomyces cerevisiae39.512e-131 420
LLPS-Cym-0782Cyanidioschyzon merolae38.591e-116 387
LLPS-Brd-2068Brachypodium distachyon35.123e-99 337
LLPS-Ora-1966Ornithorhynchus anatinus30.981e-74 266