LLPS-Drm-2399
ea

▼ OVERVIEW

Status: Reviewed
Protein Name: Serine protease easter
Gene Name: ea, CG4920
Ensembl Gene: FBgn0000533
Ensembl Protein: FBpp0082539
Organism: Drosophila melanogaster
Taxa ID: 7227
LLPS Type: LLPS regulator


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Insulator body
"...The overexpression of EAST variants resulted in complete association of dCTCF with Su in enlarged speckles, while CP190 was aggregated in independent speckles. In addition, overexpression of EAST or EAST1-1995-FLAG resulted in an increase in the size of speckles that correlated with a decrease in their number, with overexpression of EAST933-2362-FLAG having the most evident effect on speckle size."
S2 cells26489095

▼ FUNCTION

Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo (PubMed:9477324, PubMed:2107028, PubMed:12493753). Three proteases; ndl, gd and snk process easter to create active easter (PubMed:9477324). Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo (PubMed:9477324).

▼ CROSS REFERENCE

▼ SEQUENCE

Protein Sequence (FASTA)
1     MLKPSIICLF  LGILAKSSAG  QFYFPNEAAQ  VPNYGRCITP  NRERALCIHL  EDCKYLYGLL  60
61    TTTPLRDTDR  LYLSRSQCGY  TNGKVLICCP  DRYRESSSET  TPPPKPNVTS  NSLLPLPGQC  120
121   GNILSNRIYG  GMKTKIDEFP  WMALIEYTKS  QGKKGHHCGG  SLISTRYVIT  ASHCVNGKAL  180
181   PTDWRLSGVR  LGEWDTNTNP  DCEVDVRGMK  DCAPPHLDVP  VERTIPHPDY  IPASKNQVND  240
241   IALLRLAQQV  EYTDFVRPIC  LPLDVNLRSA  TFDGITMDVA  GWGKTEQLSA  SNLKLKAAVE  300
301   GFRMDECQNV  YSSQDILLED  TQMCAGGKEG  VDSCRGDSGG  PLIGLDTNKV  NTYYFLAGVV  360
361   SFGPTPCGLA  GWPGVYTLVG  KYVDWIQNTI  ES  392
Nucleotide CDS Sequence (FASTA)
1     ATGCTAAAGC  CATCGATTAT  CTGCCTCTTT  TTGGGCATTT  TGGCGAAATC  ATCGGCGGGC  60
61    CAGTTCTACT  TCCCCAACGA  GGCGGCCCAG  GTGCCCAACT  ATGGGCGCTG  CATAACGCCG  120
121   AATCGGGAGC  GTGCGCTGTG  CATCCATCTG  GAGGACTGCA  AGTACCTATA  CGGCCTGCTG  180
181   ACCACCACCC  CGCTCCGGGA  CACGGACCGG  CTTTACCTGA  GTCGCAGCCA  GTGCGGCTAT  240
241   ACGAACGGCA  AGGTGCTCAT  CTGCTGTCCG  GACCGCTACA  GGGAGAGCTC  ATCGGAGACG  300
301   ACGCCACCGC  CCAAGCCCAA  TGTAACCAGC  AACAGCCTTC  TGCCGCTGCC  AGGACAATGC  360
361   GGCAACATCC  TGTCCAATCG  CATCTATGGC  GGAATGAAGA  CCAAGATCGA  CGAGTTCCCC  420
421   TGGATGGCTC  TAATTGAATA  CACCAAAAGC  CAGGGCAAAA  AGGGTCACCA  TTGCGGCGGA  480
481   TCCTTGATCA  GCACAAGATA  TGTGATCACA  GCCTCGCATT  GTGTCAACGG  AAAGGCACTG  540
541   CCCACCGACT  GGCGGTTGTC  CGGTGTTCGT  TTGGGCGAAT  GGGACACCAA  CACTAATCCC  600
601   GATTGCGAAG  TAGATGTGCG  CGGCATGAAG  GACTGCGCGC  CACCGCATCT  GGACGTGCCC  660
661   GTGGAGCGTA  CTATACCCCA  TCCCGACTAT  ATACCGGCCT  CCAAGAACCA  GGTCAACGAC  720
721   ATAGCCCTGC  TGCGTCTGGC  CCAGCAGGTG  GAGTACACCG  ACTTTGTGCG  ACCCATTTGC  780
781   CTGCCTCTGG  ACGTCAATCT  GCGGTCGGCC  ACCTTCGACG  GCATCACCAT  GGACGTGGCC  840
841   GGCTGGGGCA  AAACGGAGCA  GCTGTCGGCC  AGCAACCTCA  AGCTGAAGGC  AGCCGTGGAG  900
901   GGATTCCGGA  TGGATGAGTG  CCAGAACGTC  TACAGCAGCC  AGGACATTCT  CCTCGAGGAC  960
961   ACACAGATGT  GTGCCGGCGG  CAAGGAAGGC  GTCGACTCCT  GTCGCGGTGA  CTCCGGGGGA  1020
1021  CCGCTCATCG  GCTTGGACAC  CAACAAGGTG  AACACCTACT  ACTTCCTGGC  CGGCGTCGTG  1080
1081  TCATTTGGAC  CGACTCCGTG  CGGTCTGGCC  GGATGGCCAG  GAGTTTACAC  GCTGGTGGGC  1140
1141  AAGTACGTTG  ATTGGATACA  AAACACTATT  GAGTCC  1176

▼ KEYWORD

ID
Family
Calcium
Complete proteome
Developmental protein
Disulfide bond
Hydrolase
Metal-binding
Protease
Reference proteome
Secreted
Serine protease
Signal
Zymogen

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Extracellular region
Cellular Component
Extracellular space
Cellular Component
Perivitelline space
Molecular Function
Metal ion binding
Molecular Function
Serine-type endopeptidase activity
Biological Process
Dorsal/ventral axis specification
Biological Process
Dorsal/ventral pattern formation
Biological Process
Maternal specification of dorsal/ventral axis, oocyte, germ-line encoded
Biological Process
Positive regulation of Toll signaling pathway
Biological Process
Proteolysis
Biological Process
Toll signaling pathway
Biological Process
Ventral furrow formation

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Mea-1935Mesocricetus auratus34.916e-1474.3
LLPS-Caj-4269Callithrix jacchus32.921e-25 112
LLPS-Nol-3610Nomascus leucogenys32.481e-0755.5
LLPS-Ora-3455Ornithorhynchus anatinus32.451e-2197.1
LLPS-Poa-0703Pongo abelii32.365e-29 124
LLPS-Fec-3130Felis catus32.121e-0860.5
LLPS-Sah-1454Sarcophilus harrisii31.853e-1887.4
LLPS-Leo-0777Lepisosteus oculatus30.456e-2092.0
LLPS-Xet-2403Xenopus tropicalis29.961e-1682.8
LLPS-Sus-4797Sus scrofa29.924e-2092.8
LLPS-Ten-2159Tetraodon nigroviridis29.792e-1682.8
LLPS-Mum-3737Mus musculus29.775e-1989.7
LLPS-Icp-2610Ictalurus punctatus29.681e-2094.4
LLPS-Hos-2807Homo sapiens29.553e-1887.8
LLPS-Gog-2295Gorilla gorilla29.555e-1887.0
LLPS-Cea-1991Cercocebus atys29.523e-1990.5
LLPS-Scm-1963Scophthalmus maximus29.59e-2092.8
LLPS-Eqc-2785Equus caballus29.438e-1783.6
LLPS-Myl-2486Myotis lucifugus29.431e-1888.6
LLPS-Xim-1116Xiphophorus maculatus29.393e-1990.5
LLPS-Dar-3216Danio rerio29.23e-2093.2
LLPS-Otg-4670Otolemur garnettii29.173e-2093.2
LLPS-Man-2117Macaca nemestrina29.158e-1989.0
LLPS-Mam-2917Macaca mulatta29.151e-1888.2
LLPS-Maf-3947Macaca fascicularis29.159e-1989.0
LLPS-Chs-3749Chlorocebus sabaeus29.151e-1888.6
LLPS-Paa-1475Papio anubis29.062e-1888.2
LLPS-Ran-4044Rattus norvegicus28.793e-1990.1
LLPS-Orn-2834Oreochromis niloticus28.738e-1680.9
LLPS-Caf-2328Canis familiaris28.688e-1783.6
LLPS-Gaa-2232Gasterosteus aculeatus28.624e-1887.4
LLPS-Tar-3589Takifugu rubripes28.321e-1477.8
LLPS-Meg-2580Meleagris gallopavo28.225e-1372.4
LLPS-Cap-2738Cavia porcellus28.099e-1782.4
LLPS-Pat-0484Pan troglodytes28.045e-1887.0
LLPS-Bot-1799Bos taurus27.842e-1373.6
LLPS-Ict-1684Ictidomys tridecemlineatus27.683e-1990.1
LLPS-Ova-2347Ovis aries27.682e-1476.6
LLPS-Gaga-3937Gallus gallus27.593e-1168.9
LLPS-Orl-0714Oryzias latipes27.572e-1476.6
LLPS-Scf-1689Scleropages formosus26.488e-1577.8