• Disorder▼
  • Domain▼
  • PTM▼
  • Variation▼
  • Mutation▼
  • Interaction▼
  • Disease▼
  • Drug▼
  • Physicochemical▼
  • Function▼
  • Proteomics▼
  • Structure▼
  • Localization▼
  • Expression▼
  • Element▼
  • Methylation▼

LLPS-Bot-4397
USP33

Integrated Annotations

▼ OVERVIEW

Status: Unreviewed
Protein Name: Ubiquitin carboxyl-terminal hydrolase 33; Deubiquitinating enzyme 33; Ubiquitin thioesterase 33; Ubiquitin-specific-processing protease 33
Gene Name: USP33
Ensembl Gene: ENSBTAG00000020761.6
Ensembl Protein: ENSBTAP00000071855.1
Organism: Bos taurus
Taxa ID: 9913
LLPS Type: Client


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
Centrosome/Spindle pole bodyPredicted from orthologs(View)

▼ FUNCTION

Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of CCP110 in S and G2/M phase, leading to stabilize CCP110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of ROBO1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of ROBO1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (ARRB1 and ARRB2) and beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MSSFRSHCPH  LDSVGEITKE  DLIQKSHGSC  QDCKVRGPNL  WACLENRCSY  VGCGESQVDH  60
61    STIHSQETKH  YLTVNLTTLR  VWCYACSKEV  FLDRKLGTQP  SLPHVKPLHQ  IQENGVQDFK  120
121   IPSNTTLKTP  LVAVFDDLDI  EVEEEDELKA  RGLTGLKNIG  NTCYMNAALQ  ALSNCPPLTQ  180
181   FFLDCGGLAR  TDKKPAICKS  YLKLMTELWH  KSRPGSVVPT  TLFQGIKTVN  PTFRGYSQQD  240
241   AQEFLRCLMD  LLHEELKEQV  MEVEEDPQTI  MTEETMEEDK  SQSDVDFQSC  ESCSSSDKAE  300
301   NENGSRSFSE  DNNETTMLIQ  DDENNSEMSK  DWQKEKMCNK  INKVHSEGEL  DKDRDSVSET  360
361   ADLNNQETVK  VQIHSRASEY  ITDVHLNDLS  TPQILPSNEG  VNPRLSASPP  KSGNLWPGLP  420
421   PTHKKVQSAL  SPKRKKQHKK  YRSVISDIFD  GTIISSVQCL  TCDRVSVTLE  TFQDLSLPIP  480
481   GKEDLAKLHS  SSHPTSIVKA  GSCGEAYAPQ  GWIAFFMEYV  KRFVVSCVPS  WFWGPVVTLQ  540
541   DCLAAFFARD  ELKGDNMYSC  EKCKKLRNGV  KFCKVQKFPE  ILCIHLKRFR  HELMFSTKIS  600
601   THVSFPLEGL  DLQPFLAKDS  PVQIVTYDLL  SVICHHGTAS  SGHYIAYCRN  NLNNLWYEFD  660
661   DQSVTEVSES  TVQNAEAYVL  FYRKSSEEAQ  KERRRISNLL  NIMEPSLLQF  YISRQWLNKF  720
721   KTFAEPGPIS  NNDFLCIHGG  VPPRKAGYIE  DLVLMLPQNI  WDNLYSRYGG  GPAVNHLYIC  780
781   HTCQIEAEKI  EKRRKTELEI  FIRLNRAFQE  EDSPATFYCI  SMQWFREWES  FVKGKDGDPP  840
841   GPIDNTKIAV  TKCGNVILRQ  GADSGQISEE  TWNFLQSIYG  GGPEVILRPP  VVHVDPDAVQ  900
901   AEEKIEVETR  SL  912
Nucleotide CDS Sequence (FASTA)
1     ATGTCATCTT  TTCGAAGTCA  TTGTCCACAT  TTGGATTCAG  TTGGTGAAAT  AACTAAAGAA  60
61    GATTTAATAC  AAAAATCTCA  TGGTTCTTGT  CAGGATTGTA  AAGTCAGAGG  ACCAAACCTT  120
121   TGGGCATGCC  TGGAGAATAG  GTGTTCATAT  GTTGGCTGTG  GTGAATCACA  AGTGGATCAT  180
181   AGCACCATAC  ATTCTCAGGA  GACAAAGCAT  TATCTAACTG  TGAACCTTAC  CACCCTTCGA  240
241   GTTTGGTGTT  ATGCTTGCAG  CAAAGAAGTA  TTTTTGGATA  GGAAATTAGG  AACTCAGCCT  300
301   TCATTACCTC  ATGTAAAACC  ACTTCACCAA  ATACAAGAAA  ATGGCGTACA  GGATTTTAAA  360
361   ATACCCAGTA  ACACAACATT  AAAAACTCCT  CTGGTTGCTG  TGTTTGATGA  TCTGGATATA  420
421   GAAGTAGAAG  AAGAAGATGA  ACTTAAGGCA  AGAGGTCTTA  CAGGTTTGAA  AAATATTGGA  480
481   AATACTTGTT  ATATGAATGC  AGCTTTGCAG  GCTCTTTCTA  ACTGCCCGCC  TTTGACGCAG  540
541   TTTTTTCTTG  ATTGTGGAGG  ACTGGCTCGA  ACAGATAAGA  AACCAGCTAT  TTGTAAAAGT  600
601   TATCTCAAAC  TGATGACAGA  GCTCTGGCAT  AAAAGCAGGC  CAGGATCAGT  TGTGCCTACT  660
661   ACTCTGTTTC  AAGGAATTAA  AACTGTAAAT  CCAACATTTC  GGGGGTATTC  TCAGCAGGAT  720
721   GCTCAAGAAT  TCCTTCGATG  TTTAATGGAT  CTGCTTCATG  AAGAATTGAA  AGAACAAGTC  780
781   ATGGAAGTAG  AAGAGGATCC  TCAAACTATA  ATGACTGAAG  AGACCATGGA  GGAAGACAAG  840
841   AGCCAGTCAG  ATGTAGATTT  TCAGTCCTGC  GAATCTTGTA  GCAGCAGTGA  TAAAGCTGAA  900
901   AATGAAAATG  GCTCTAGAAG  CTTTTCTGAA  GATAATAATG  AGACAACAAT  GTTAATTCAG  960
961   GATGATGAGA  ACAATTCAGA  AATGTCAAAA  GATTGGCAAA  AAGAGAAGAT  GTGCAACAAG  1020
1021  ATTAATAAAG  TACATTCTGA  AGGAGAATTA  GATAAAGATA  GAGACTCTGT  ATCTGAAACA  1080
1081  GCTGATTTGA  ACAACCAGGA  AACTGTCAAA  GTGCAAATAC  ACAGCAGAGC  TTCAGAATAT  1140
1141  ATTACTGATG  TCCATTTGAA  TGACCTGTCT  ACACCACAGA  TCCTCCCATC  AAATGAAGGT  1200
1201  GTTAATCCAA  GATTATCAGC  AAGCCCTCCT  AAATCAGGCA  ATTTGTGGCC  AGGATTGCCA  1260
1261  CCCACACACA  AAAAAGTTCA  ATCTGCATTA  TCTCCAAAGA  GAAAAAAACA  GCACAAGAAA  1320
1321  TACCGAAGTG  TTATTTCAGA  CATATTTGAT  GGAACAATCA  TTAGTTCAGT  TCAGTGTCTG  1380
1381  ACTTGTGACA  GGGTGTCTGT  AACCCTCGAG  ACCTTTCAAG  ATCTGTCCTT  GCCAATTCCT  1440
1441  GGCAAGGAAG  ACCTTGCTAA  ACTGCATTCG  TCAAGTCATC  CAACATCTAT  AGTCAAAGCA  1500
1501  GGATCATGTG  GCGAAGCATA  TGCTCCACAA  GGGTGGATAG  CTTTTTTCAT  GGAGTATGTG  1560
1561  AAGAGGTTTG  TTGTCTCATG  TGTCCCTAGC  TGGTTTTGGG  GTCCAGTAGT  AACCTTGCAA  1620
1621  GATTGTCTTG  CTGCCTTCTT  TGCCAGAGAT  GAACTAAAAG  GTGACAATAT  GTACAGTTGT  1680
1681  GAAAAATGCA  AAAAGTTGAG  AAATGGAGTA  AAGTTTTGTA  AAGTACAAAA  GTTTCCTGAG  1740
1741  ATTTTGTGCA  TCCATCTTAA  AAGATTCAGA  CATGAACTGA  TGTTTTCCAC  CAAAATCAGT  1800
1801  ACCCATGTTT  CATTTCCACT  GGAAGGCCTG  GATCTTCAAC  CATTTCTTGC  TAAAGATAGT  1860
1861  CCGGTGCAAA  TTGTTACCTA  TGATCTTCTG  TCAGTCATTT  GTCACCATGG  AACTGCAAGT  1920
1921  AGTGGACACT  ATATTGCCTA  CTGCCGAAAC  AATTTAAATA  ATCTCTGGTA  TGAATTTGAC  1980
1981  GACCAGAGTG  TCACTGAAGT  TTCAGAGTCT  ACTGTACAAA  ATGCAGAAGC  TTATGTTCTC  2040
2041  TTCTACAGGA  AGAGCAGTGA  AGAAGCACAA  AAAGAGAGGC  GAAGAATATC  AAATTTGTTG  2100
2101  AACATAATGG  AACCAAGTCT  CCTTCAGTTT  TATATTTCTC  GACAGTGGCT  AAATAAATTT  2160
2161  AAGACCTTTG  CTGAACCTGG  CCCTATTTCA  AATAATGATT  TTCTCTGTAT  TCATGGAGGT  2220
2221  GTTCCTCCAC  GAAAAGCTGG  TTATATTGAA  GACCTGGTTT  TGATGCTGCC  TCAGAACATT  2280
2281  TGGGATAACC  TATATAGCAG  GTATGGAGGA  GGACCAGCCG  TCAACCATCT  GTACATTTGT  2340
2341  CACACTTGCC  AGATTGAAGC  GGAGAAAATT  GAAAAAAGAA  GAAAAACTGA  ATTGGAAATT  2400
2401  TTTATTCGGC  TCAACAGAGC  ATTCCAAGAG  GAAGACTCTC  CAGCTACTTT  TTATTGCATC  2460
2461  AGTATGCAAT  GGTTTAGAGA  ATGGGAAAGT  TTTGTGAAGG  GTAAAGATGG  AGATCCTCCA  2520
2521  GGTCCTATTG  ATAACACTAA  AATTGCAGTC  ACCAAATGTG  GCAATGTGAT  ACTCAGGCAA  2580
2581  GGAGCAGATT  CTGGTCAGAT  TTCTGAAGAA  ACATGGAATT  TTCTACAGTC  TATATATGGT  2640
2641  GGAGGGCCCG  AAGTTATCTT  GCGGCCTCCA  GTTGTTCATG  TTGATCCTGA  TGCAGTACAA  2700
2701  GCAGAAGAAA  AAATTGAAGT  AGAAACTCGG  TCTTTGTAA  2739

▼ KEYWORD

ID
Family
Complete proteome
Cytoplasm
Cytoskeleton
Endocytosis
Hydrolase
Metal-binding
Phosphoprotein
Protease
Reference proteome
Repeat
Thiol protease
Ubl conjugation
Ubl conjugation pathway
Zinc
Zinc-finger

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cell body
Cellular Component
Centrosome
Cellular Component
Cytoplasm
Cellular Component
Focal adhesion
Cellular Component
Golgi apparatus
Cellular Component
Nucleoplasm
Cellular Component
Perinuclear region of cytoplasm
Molecular Function
Cysteine-type endopeptidase activity
Molecular Function
G protein-coupled receptor binding
Molecular Function
Ral GTPase binding
Molecular Function
Thiol-dependent ubiquitin-specific protease activity
Molecular Function
Thiol-dependent ubiquitinyl hydrolase activity
Molecular Function
Zinc ion binding
Biological Process
Axon guidance
Biological Process
Cell migration
Biological Process
Cellular response to starvation
Biological Process
Centrosome duplication
Biological Process
Endocytosis
Biological Process
Negative regulation of protein binding
Biological Process
Positive regulation of protein binding
Biological Process
Protein deubiquitination
Biological Process
Protein K48-linked deubiquitination
Biological Process
Protein K63-linked deubiquitination
Biological Process
Protein stabilization
Biological Process
Regulation of autophagy
Biological Process
Regulation of G protein-coupled receptor signaling pathway
Biological Process
Ubiquitin-dependent protein catabolic process

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Ova-2242Ovis aries99.560.01843
LLPS-Fec-1387Felis catus97.590.01786
LLPS-Urm-3226Ursus maritimus97.590.01791
LLPS-Aim-4038Ailuropoda melanoleuca97.270.01785
LLPS-Man-3019Macaca nemestrina97.260.01780
LLPS-Mam-4151Macaca mulatta97.260.01780
LLPS-Mal-2954Mandrillus leucophaeus97.260.01780
LLPS-Paa-4395Papio anubis97.260.01780
LLPS-Mup-4385Mustela putorius furo97.190.01498
LLPS-Chs-3694Chlorocebus sabaeus97.040.01776
LLPS-Caf-3738Canis familiaris96.940.01778
LLPS-Gog-3750Gorilla gorilla96.820.01775
LLPS-Pat-1407Pan troglodytes96.710.01774
LLPS-Pap-1593Pan paniscus96.710.01774
LLPS-Poa-3232Pongo abelii96.710.01766
LLPS-Hos-2642Homo sapiens96.60.01770
LLPS-Caj-1872Callithrix jacchus96.60.01792
LLPS-Eqc-2768Equus caballus96.490.01775
LLPS-Ict-4608Ictidomys tridecemlineatus96.060.01749
LLPS-Fud-0376Fukomys damarensis95.610.01774
LLPS-Maf-0787Macaca fascicularis95.50.01739
LLPS-Otg-3121Otolemur garnettii95.40.01750
LLPS-Cas-2274Carlito syrichta95.390.01724
LLPS-Cea-1550Cercocebus atys95.290.01735
LLPS-Myl-0508Myotis lucifugus95.20.01770
LLPS-Loa-3640Loxodonta africana94.970.01764
LLPS-Aon-0963Aotus nancymaae94.740.01731
LLPS-Mea-3910Mesocricetus auratus93.070.01562
LLPS-Rhb-2688Rhinopithecus bieti92.960.01668
LLPS-Orc-4277Oryctolagus cuniculus92.830.01705
LLPS-Ran-2203Rattus norvegicus92.770.01689
LLPS-Mum-3264Mus musculus92.00.01684
LLPS-Nol-1368Nomascus leucogenys90.790.01679
LLPS-Cap-3482Cavia porcellus86.620.01543
LLPS-Ora-3348Ornithorhynchus anatinus85.670.01072
LLPS-Tag-1087Taeniopygia guttata83.840.01471
LLPS-Anp-2796Anas platyrhynchos83.260.01522
LLPS-Pes-1527Pelodiscus sinensis83.060.01529
LLPS-Meg-0398Meleagris gallopavo82.750.01292
LLPS-Gaga-3823Gallus gallus82.170.01500
LLPS-Fia-2009Ficedula albicollis82.150.01495
LLPS-Sah-3445Sarcophilus harrisii77.910.01406
LLPS-Anc-0872Anolis carolinensis77.240.01395
LLPS-Xet-1779Xenopus tropicalis76.280.01375
LLPS-Leo-2514Lepisosteus oculatus72.890.01328
LLPS-Scf-3784Scleropages formosus72.250.01299
LLPS-Asm-1612Astyanax mexicanus71.070.01296
LLPS-Orn-0259Oreochromis niloticus70.820.01315
LLPS-Lac-0299Latimeria chalumnae70.810.01311
LLPS-Xim-2017Xiphophorus maculatus70.430.01303
LLPS-Dar-1380Danio rerio70.310.01289
LLPS-Scm-3722Scophthalmus maximus70.280.01298
LLPS-Pof-4055Poecilia formosa70.080.01296
LLPS-Gaa-3416Gasterosteus aculeatus69.820.01293
LLPS-Tar-3838Takifugu rubripes67.320.01249
LLPS-Orl-3281Oryzias latipes66.60.01266
LLPS-Drm-1407Drosophila melanogaster51.797e-26 119
LLPS-Icp-0185Ictalurus punctatus42.732e-1584.7
LLPS-Sus-3989Sus scrofa40.741e-1379.0
LLPS-Dio-2970Dipodomys ordii39.812e-1172.0
LLPS-Bro-1423Brassica oleracea39.642e-1275.5
LLPS-Cii-2281Ciona intestinalis39.622e-1273.9
LLPS-Cis-0575Ciona savignyi39.629e-1376.3
LLPS-Ten-0682Tetraodon nigroviridis39.094e-1171.6
LLPS-Mod-2726Monodelphis domestica38.894e-1171.6
LLPS-Tra-0734Triticum aestivum35.511e-0966.6
LLPS-Via-2031Vigna angularis34.074e-27 122
LLPS-Abg-1073Absidia glauca32.682e-28 127
LLPS-Gas-0995Galdieria sulphuraria32.46e-46 180
LLPS-Pug-1167Puccinia graminis30.831e-28 128
LLPS-Sob-0910Sorghum bicolor26.357e-33 141