※ Paraspeckle introduction

    Paraspeckles are mammalian-specific nuclear bodies that often lie adjacent to splicing speckles, containing several multifunctional proteins, including PSF (PTB-associated splicing factor), p54nrb, PSP1andRBM14/CoAA/PSP2 (here- after referred to CoAA) (1), the long noncoding RNA NEAT1 assembles these protein components and is critical for paraspeckle formation. DIS3 was proved to be involved in controlling the formation of paraspeckles, nuclear bodies that are organized around NEAT1 lncRNA, whose short form was overexpressed in cells with mutated DIS3 (2). Paraspeckles regulate gene expression through a number of mechanisms, including sequestration of specific transcription factors and/or hyperedited mRNAs (3). Besides, RNA editing by the enzyme ADAR (Adenosine deaminase acting on RNA) was proved to occur in paraspeckles (4).

Reference
1. Chang, S. H., Chang, W. L., Lu, C. C., & Tarn, W. Y. (2014) Alanine repeats influence protein localization in splicing speckles and paraspeckles. Nucleic Acids Res, 42(22), 13788-13798. PMID: 25414336
2. Szczepinska, T., Kalisiak, K., Tomecki, R., Labno, A., Borowski, L. S., Kulinski, T. M., et al. (2015) DIS3 shapes the RNA polymerase II transcriptome in humans by degrading a variety of unwanted transcripts. Genome Res, 25(11), 1622-1633. PMID: 26294688
3. Adriaens, C., Standaert, L., Barra, J., Latil, M., Verfaillie, A., Kalev, P., et al. (2016) p53 induces formation of NEAT1 lncRNA-containing paraspeckles that modulate replication stress response and chemosensitivity. Nat Med, 22(8), 861-868. PMID: 27376578
4. Banerjee, A., Vest, K. E., Pavlath, G. K., & Corbett, A. H. (2017) Nuclear poly(A) binding protein 1 (PABPN1) and Matrin3 interact in muscle cells and regulate RNA processing. Nucleic Acids Res, 45(18), 10706-10725. PMID: 28977530