※ Nuclear stress body introduction

    Nuclear stress bodies/granule (nSBs), also called perichromatin granules (PGs), are a kind of cytoplasmic PMLOs, which are different in size, shape, and composition, and almost invariantly contain intrinsically disordered proteins (1). They are formed as a result of highly regulated and reversible liquid-liquid demixing phase separation and represent condensed liquid droplets, which are invariantly enriched in intrinsically disordered proteins. Perichromatin granules are located in nucleus with pre-mRNA, SatIII DNA, SatIII ncRNAs, chromatin components and it’s proved to be related to the response to heat shock and other forms of stress (1). The assembly of these bodies requires the synthesis of non-coding RNAs with a probable role in the post-transcriptional regulation of gene expression and in higher-order chromatin organization (2).

Reference
1. Golding, M., Ruhrberg, C., Sandle, J., & Gullick, W. J. (2004) Mapping nucleolar and spliceosome localization sequences of neuregulin1-beta3. Exp Cell Res, 299(1), 110-8. PMID: 15302578
1. Uversky, V. N. (2017) Intrinsically disordered proteins in overcrowded milieu: Membrane-less organelles, phase separation, and intrinsic disorder. Curr Opin Struct Biol, 44, 18-30. PMID: 27838525
2. Biamonti, G. (2004) Nuclear stress bodies: a heterochromatin affair? Nat Rev Mol Cell Biol, 5(6), 493-498. PMID: 15173828