※ Insulator body introduction

    Eukaryotic cells are inhomogeneously crowded with biological macromolecules (proteins and nucleic acids) and often contain numerous cellular bodies, including proteinaceous membrane-less organelles (PMLOs), They are formed as a result of highly regulated and reversible liquid-liquid demixing phase separation and represent condensed liquid droplets, which are invariantly enriched in intrinsically disordered proteins (1). Insulator bodies is a kind of these bodies located in nucleus without any DNA or RNA components and it’s periphery-associated, proved to be related to the response to osmotic stress (1). Under the osmotic stress, Chromatin insulators assist in the formation of higher-order chromatin structures by mediating long-range contacts between distant genomic sites, It has been suggested that insulators accomplish this task by forming dense nuclear foci termed insulator bodies that result from the coalescence of multiple protein-bound insulators (2).

Reference
1. Uversky, V. N. (2017) Intrinsically disordered proteins in overcrowded milieu: Membrane-less organelles, phase separation, and intrinsic disorder. Curr Opin Struct Biol, 44, 18-30. PMID: 27838525
2. Schoborg, T., Rickels, R., Barrios, J., & Labrador, M. (2013) Chromatin insulator bodies are nuclear structures that form in response to osmotic stress and cell death. J Cell Biol, 202(2), 261-276. PMID: 23878275