※ Droplet introduction

    Interactions between diverse synthetic, multivalent macromolecules (including multi-domain proteins and RNA) produce sharp liquid-liquid-demixing phase separations, generating micrometre-sized liquid droplets in aqueous solution (1-2). In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments. By now, various proteins have been purified and tested for their ability to phase separate and form liquid condensates in vitro (3).

References
1. Li P., Banjade S., Cheng H.C., et al. (2012) Phase transitions in the assembly of multivalent signalling proteins. Nature, 483, 336-40. PMID: 22398450
2. Kato M., Han T.W., Xie S., et al. (2012) Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Cell, 149, 753-67. PMID: 22579281
3. Alberti S., Saha S., Woodruff J.B., et al. (2018) A User’s Guide for Phase Separation Assays with Purified Proteins. J. Mol. Biol., 430, 4806-4820. PMID: 29944854

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No.	Status	DrLLPS ID	Ensemble Gene ID	UniProt Accession	Gene Name
1		LLPS-Tar-0246	ENSTRUG00000006269.2	P61944
2		LLPS-Tar-0499	ENSTRUG00000025576.1	A0A3B5JWN6
3		LLPS-Tar-0724	ENSTRUG00000021548.1	A0A3B5K9S8
4		LLPS-Tar-0889	ENSTRUG00000017345.2	H2V5F6
5		LLPS-Tar-3788	ENSTRUG00000003170.2	H2S4W1
6		LLPS-Tar-1932	ENSTRUG00000007370.2	H2T0M8	ago2
7		LLPS-Tar-0250	ENSTRUG00000001235.2	H2RRV5	ak2
8		LLPS-Tar-0650	ENSTRUG00000016801.2	H2V1G0	atxn2
9		LLPS-Tar-0472	ENSTRUG00000013307.2	H2UAB0	aurka
10		LLPS-Tar-3292	ENSTRUG00000018591.2	H2UVZ0	camk2b
11		LLPS-Tar-2465	ENSTRUG00000012203.2	H2U1U1	cbx1
12		LLPS-Tar-0377	ENSTRUG00000023866.1	A0A3B5KDF0	cbx2
13		LLPS-Tar-1959	ENSTRUG00000003115.2	H2S4H2	cbx5
14		LLPS-Tar-2476	ENSTRUG00000000552.2	H2RMD8	ccnt1
15		LLPS-Tar-3266	ENSTRUG00000010233.2	H2TM74	cdk7
16		LLPS-Tar-1531	ENSTRUG00000008976.2	H2TD26	cdk9
17		LLPS-Tar-3261	ENSTRUG00000010187.2	A0A3B5K9X3	cirbp
18		LLPS-Tar-0269	ENSTRUG00000015453.2	H2URF6	ckap5
19		LLPS-Tar-3079	ENSTRUG00000022887.1	A0A3B5JYH1	cpeb2
20		LLPS-Tar-1357	ENSTRUG00000004323.2	H2SCX1	cstf2
21		LLPS-Tar-2179	ENSTRUG00000022353.1	A0A3B5KHG9	daxx
22		LLPS-Tar-1050	ENSTRUG00000002467.2	H2RZY2	dazap1
23		LLPS-Tar-0617	ENSTRUG00000003429.2	H2S6N4	dcp1a
24		LLPS-Tar-1154	ENSTRUG00000011848.2	A0A3B5KEL6	ddx6
25		LLPS-Tar-3672	ENSTRUG00000017849.2	H2V988	dlgap1
26		LLPS-Tar-3086	ENSTRUG00000006869.2	H2SWR8	dyrk1a
27		LLPS-Tar-1369	ENSTRUG00000017078.2	H2V3H2	edc3
28		LLPS-Tar-0788	ENSTRUG00000011593.2	H2TX97	eif4g3
29		LLPS-Tar-2522	ENSTRUG00000007941.2	A0A3B5K2D6	esrp2
30		LLPS-Tar-0017	ENSTRUG00000004408.2	A0A3B5KQ98	fbl
31		LLPS-Tar-1404	ENSTRUG00000020580.1	A0A3B5K6U8	gar1
32		LLPS-Tar-1144	ENSTRUG00000009789.2	A0A3B5JYA3	gnl2
33		LLPS-Tar-3979	ENSTRUG00000008254.2	H2T7L9	grb2
34		LLPS-Tar-0390	ENSTRUG00000025814.1	A0A3B5KAF5	homer3
35		LLPS-Tar-0738	ENSTRUG00000007387.2	A0A3B5JV16	ipo5
36		LLPS-Tar-0586	ENSTRUG00000008630.2	H2TAF8	kpna2
37		LLPS-Tar-0074	ENSTRUG00000000542.2	A0A3B5K7P8	kpnb1
38		LLPS-Tar-3043	ENSTRUG00000023625.1	A0A3B5KPC4	LOC101061771
39		LLPS-Tar-3942	ENSTRUG00000023619.1	A0A3B5KLW9	LOC101061790
40		LLPS-Tar-2836	ENSTRUG00000018585.2	H2VEE6	LOC101062087
41		LLPS-Tar-3791	ENSTRUG00000014739.2	H2UL58	LOC101063191
42		LLPS-Tar-1670	ENSTRUG00000018450.2	H2VDE0	LOC101066421
43		LLPS-Tar-3718	ENSTRUG00000021399.1	A0A3B5KDY3	LOC101068354
44		LLPS-Tar-0448	ENSTRUG00000016634.2	A0A3B5KGB7	LOC101069458
45		LLPS-Tar-0523	ENSTRUG00000011335.2	H2TV99	LOC101071804
46		LLPS-Tar-1200	ENSTRUG00000012645.2	H2U4Y5	LOC101072838
47		LLPS-Tar-1264	ENSTRUG00000005571.2	A0A3B5KIP3	LOC101073809
48		LLPS-Tar-0880	ENSTRUG00000005680.2	H2SMX5	LOC101074799
49		LLPS-Tar-a1000	ENSTRUG00000015442.2	H2URA5	LOC101076067
50		LLPS-Tar-0701	ENSTRUG00000004447.2	H2SDU3	LOC101077097
51		LLPS-Tar-0915	ENSTRUG00000021494.1	A0A3B5K0I1	LOC101079154
52		LLPS-Tar-2446	ENSTRUG00000001050.2	H2RQP7	LOC101079186
53		LLPS-Tar-0408	ENSTRUG00000016826.2	H2V1M8	LOC101079306
54		LLPS-Tar-3395	ENSTRUG00000004651.2	Q8QGU8	LOC445899
55		LLPS-Tar-1167	ENSTRUG00000021737.1	A0A3B5K599	lsm4
56		LLPS-Tar-0898	ENSTRUG00000025106.1	A0A3B5K5D5	map1lc3b
57		LLPS-Tar-0806	ENSTRUG00000017728.2	A0A3B5K239	nck1
58		LLPS-Tar-2510	ENSTRUG00000000175.2	H2RJP9	nphs1
59		LLPS-Tar-1843	ENSTRUG00000024003.1	A0A3B5KH27	npm1
60		LLPS-Tar-1566	ENSTRUG00000010163.2	H2TLM9	nup153
61		LLPS-Tar-0530	ENSTRUG00000017712.2	H2V866	NUP62
62		LLPS-Tar-0490	ENSTRUG00000002669.2	H2S1E7	nup98
63		LLPS-Tar-1585	ENSTRUG00000011577.2	H2TX53	pabpc1
64		LLPS-Tar-0811	ENSTRUG00000014787.2	H2ULF7	PLK1
65		LLPS-Tar-0428	ENSTRUG00000008121.2	H2T6K1	pnpla6
66		LLPS-Tar-1555	ENSTRUG00000016384.2	H2UY55	prkaa2
67		LLPS-Tar-1775	ENSTRUG00000012105.2	H2U127	prmt1
68		LLPS-Tar-1481	ENSTRUG00000011360.2	A0A3B5KGS0	ptbp1
69		LLPS-Tar-1018	ENSTRUG00000020940.1	A0A3B5K9S6	rbfox1
70		LLPS-Tar-0626	ENSTRUG00000017075.2	A0A3B5JY71	rpl23a
71		LLPS-Tar-2588	ENSTRUG00000000524.2	H2RM82	RPL5 (1 of many)
72		LLPS-Tar-2798	ENSTRUG00000018124.2	H2VB04	sgo1
73		LLPS-Tar-2387	ENSTRUG00000013953.2	H2UFF8	SOS1
74		LLPS-Tar-1640	ENSTRUG00000023872.1	A0A3B5KH04	surf6
75		LLPS-Tar-0141	ENSTRUG00000007778.2	H2T3U0	syn1
76		LLPS-Tar-0125	ENSTRUG00000012070.2	A0A3B5K5V3	tardbp
77		LLPS-Tar-0015	ENSTRUG00000015136.2	A0A3B5KTG3	tia1
78		LLPS-Tar-0803	ENSTRUG00000020927.1	A0A3B5K5P6	TIAL1
79		LLPS-Tar-1950	ENSTRUG00000018491.2	H2VDP0	tnpo1
80		LLPS-Tar-2919	ENSTRUG00000018309.2	H2VC98	ubxn6
81		LLPS-Tar-3313	ENSTRUG00000021140.1	A0A3B5JYG3	znf207