※ Droplet introduction

    Interactions between diverse synthetic, multivalent macromolecules (including multi-domain proteins and RNA) produce sharp liquid-liquid-demixing phase separations, generating micrometre-sized liquid droplets in aqueous solution (1-2). In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments. By now, various proteins have been purified and tested for their ability to phase separate and form liquid condensates in vitro (3).

References
1. Li P., Banjade S., Cheng H.C., et al. (2012) Phase transitions in the assembly of multivalent signalling proteins. Nature, 483, 336-40. PMID: 22398450
2. Kato M., Han T.W., Xie S., et al. (2012) Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Cell, 149, 753-67. PMID: 22579281
3. Alberti S., Saha S., Woodruff J.B., et al. (2018) A User’s Guide for Phase Separation Assays with Purified Proteins. J. Mol. Biol., 430, 4806-4820. PMID: 29944854

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No.	Status	DrLLPS ID	Ensemble Gene ID	UniProt Accession	Gene Name
1		LLPS-Tag-0073	ENSTGUG00000005438.1	H0Z4U8
2		LLPS-Tag-0598	ENSTGUG00000010459.1	H0ZJL6
3		LLPS-Tag-0984	ENSTGUG00000001123.1	H0YS97
4		LLPS-Tag-1334	ENSTGUG00000000869.1	H0YRI3
5		LLPS-Tag-2188	ENSTGUG00000001022.1	H0YRZ5
6		LLPS-Tag-2680	ENSTGUG00000003359.1	H0YYU1
7		LLPS-Tag-3002	ENSTGUG00000001080.1	H0YS55
8		LLPS-Tag-3649	ENSTGUG00000010138.1	H0ZIR8
9		LLPS-Tag-0037	ENSTGUG00000012652.1	H0ZQY8	AGO2
10		LLPS-Tag-2993	ENSTGUG00000001332.1	H0YSW4	AK2
11		LLPS-Tag-2371	ENSTGUG00000013557.1	H0ZTL2	APP
12		LLPS-Tag-1976	ENSTGUG00000006497.1	H0Z7Z3	ATXN2
13		LLPS-Tag-0143	ENSTGUG00000008457.1	H0ZDQ2	AURKA
14		LLPS-Tag-1205	ENSTGUG00000001401.1	H0YT34	C9orf72
15		LLPS-Tag-3304	ENSTGUG00000006748.1	H0Z8X5	CAMK2G
16		LLPS-Tag-1939	ENSTGUG00000014894.1	H0ZXA3	CBX1
17		LLPS-Tag-3083	ENSTGUG00000003161.1	H0YY78	CBX2
18		LLPS-Tag-2683	ENSTGUG00000015950.1	H1A084	CCNT1
19		LLPS-Tag-0161	ENSTGUG00000014190.1	H0ZVE4	CDK7
20		LLPS-Tag-0513	ENSTGUG00000003597.1	H0YZG4	CDK9
21		LLPS-Tag-0075	ENSTGUG00000000884.1	H0YRK3	CIRBP
22		LLPS-Tag-2149	ENSTGUG00000009822.1	H0ZHS2	CPEB2
23		LLPS-Tag-0802	ENSTGUG00000002253.1	H0YVM4	CSTF2
24		LLPS-Tag-1170	ENSTGUG00000005520.1	H0Z578	CTTN
25		LLPS-Tag-1875	ENSTGUG00000006752.1	H0Z8P3	DAB2IP
26		LLPS-Tag-1070	ENSTGUG00000012575.1	H0ZQQ6	DCP1B
27		LLPS-Tag-0090	ENSTGUG00000001035.1	H0YRZ8	DCP2
28		LLPS-Tag-0347	ENSTGUG00000002548.1	H0YWF8	DDX4
29		LLPS-Tag-0668	ENSTGUG00000017360.1	H1A423	DDX6
30		LLPS-Tag-1938	ENSTGUG00000010015.1	H0ZIB6	DLGAP1
31		LLPS-Tag-0956	ENSTGUG00000004960.1	H0Z3I7	DYRK1A
32		LLPS-Tag-0050	ENSTGUG00000004007.1	H0Z0N5	EDC3
33		LLPS-Tag-0486	ENSTGUG00000002122.1	H0YV91	EIF4G3
34		LLPS-Tag-1120	ENSTGUG00000011907.1	H0ZNU1	ESRP1
35		LLPS-Tag-1079	ENSTGUG00000010419.1	H0ZJJ7	EWSR1
36		LLPS-Tag-2305	ENSTGUG00000004735.1	H0Z2U8	FMR1
37		LLPS-Tag-2480	ENSTGUG00000011998.1	H0ZP43	FYN
38		LLPS-Tag-0537	ENSTGUG00000004185.1	H0Z164	GAR1
39		LLPS-Tag-0405	ENSTGUG00000001547.1	H0YTI9	GNL2
40		LLPS-Tag-1741	ENSTGUG00000008612.1	B5FZR7	GRB2
41		LLPS-Tag-1072	ENSTGUG00000005103.1	H0Z419	GSPT1
42		LLPS-Tag-1540	ENSTGUG00000001186.1	H0YSG3	HNRNPA0
43		LLPS-Tag-0517	ENSTGUG00000002869.1	H0YXD2	HNRNPA2B1
44		LLPS-Tag-0179	ENSTGUG00000002858.1	H0YXC8	HNRNPD
45		LLPS-Tag-0765	ENSTGUG00000002850.1	H0YXB3	HNRNPDL
46		LLPS-Tag-2682	ENSTGUG00000003609.1	H0YZK6	HNRNPH3
47		LLPS-Tag-0108	ENSTGUG00000011084.1	H0ZLG9	IPO5
48		LLPS-Tag-1130	ENSTGUG00000000495.1	H0YQH7	ITSN1
49		LLPS-Tag-1745	ENSTGUG00000004534.1	H0Z2A4	KPNA2
50		LLPS-Tag-0169	ENSTGUG00000004083.1	H0Z0X1	LBR
51		LLPS-Tag-0556	ENSTGUG00000013077.1	H0ZS69	LGALS3
52		LLPS-Tag-0253	ENSTGUG00000001252.1	H0YSN1	LOC100223131
53		LLPS-Tag-2966	ENSTGUG00000001027.1	H0YRZ2	LSM4
54		LLPS-Tag-0840	ENSTGUG00000003576.1	H0YZE2	MAP1LC3A
55		LLPS-Tag-0440	ENSTGUG00000007601.1	B5G485	MAP1LC3B
56		LLPS-Tag-1642	ENSTGUG00000001908.1	H0YUJ8	MAPT
57		LLPS-Tag-2422	ENSTGUG00000002873.1	H0YXD7	NBR1
58		LLPS-Tag-2320	ENSTGUG00000005917.1	H0Z6B1	NUP153
59		LLPS-Tag-1604	ENSTGUG00000006447.1	H0Z7U1	NUP62
60		LLPS-Tag-1117	ENSTGUG00000000556.1	H0YQL8	NUP98
61		LLPS-Tag-0703	ENSTGUG00000012067.1	H0ZPA7	PABPC1
62		LLPS-Tag-0182	ENSTGUG00000004376.1	H0Z1U5	PKM
63		LLPS-Tag-1999	ENSTGUG00000006112.1	H0Z6U1	PLK1
64		LLPS-Tag-2183	ENSTGUG00000009569.1	H0ZH19	PRKAA2
65		LLPS-Tag-0946	ENSTGUG00000011295.1	H0ZM27	PSPC1
66		LLPS-Tag-0649	ENSTGUG00000000641.1	H0YQV3	PTBP1
67		LLPS-Tag-3364	ENSTGUG00000004562.1	H0Z2C9	RBFOX1
68		LLPS-Tag-0688	ENSTGUG00000006170.1	H0Z6Z7	RPL23A
69		LLPS-Tag-0392	ENSTGUG00000005988.1	H0Z6H4	RPL5
70		LLPS-Tag-3399	ENSTGUG00000002040.1	H0YV12	SOS1
71		LLPS-Tag-0852	ENSTGUG00000003255.1	H0YYI1	TARDBP
72		LLPS-Tag-1121	ENSTGUG00000017307.1	H1A3X2	TIA1
73		LLPS-Tag-1092	ENSTGUG00000000813.1	H0YRC5	UBXN6
74		LLPS-Tag-1465	ENSTGUG00000004276.1	H0Z1K4	WASL
75		LLPS-Tag-1499	ENSTGUG00000006512.1	H0Z824	XPO1
76		LLPS-Tag-1560	ENSTGUG00000004789.1	H0Z306	ZNF207