※ Droplet introduction

    Interactions between diverse synthetic, multivalent macromolecules (including multi-domain proteins and RNA) produce sharp liquid-liquid-demixing phase separations, generating micrometre-sized liquid droplets in aqueous solution (1-2). In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments. By now, various proteins have been purified and tested for their ability to phase separate and form liquid condensates in vitro (3).

References
1. Li P., Banjade S., Cheng H.C., et al. (2012) Phase transitions in the assembly of multivalent signalling proteins. Nature, 483, 336-40. PMID: 22398450
2. Kato M., Han T.W., Xie S., et al. (2012) Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Cell, 149, 753-67. PMID: 22579281
3. Alberti S., Saha S., Woodruff J.B., et al. (2018) A User’s Guide for Phase Separation Assays with Purified Proteins. J. Mol. Biol., 430, 4806-4820. PMID: 29944854

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No.	Status	DrLLPS ID	Ensemble Gene ID	UniProt Accession	Gene Name
1		LLPS-Sus-2235	ENSSSCG00000036878.1	A0A286ZUP7
2		LLPS-Sus-0984	ENSSSCG00000005935.3	A0A287B5S7	AGO2
3		LLPS-Sus-4451	ENSSSCG00000032067.1	A0A287AH85	AK2
4		LLPS-Sus-1763	ENSSSCG00000012022.3	P79307	APP
5		LLPS-Sus-2301	ENSSSCG00000009834.3	A0A286ZI01	ATXN2
6		LLPS-Sus-0282	ENSSSCG00000021845.2	A0A287AV57	ATXN2L
7		LLPS-Sus-2126	ENSSSCG00000007493.4	A5GFW1	AURKA
8		LLPS-Sus-1847	ENSSSCG00000028924.2	I3LFX5	AURKB
9		LLPS-Sus-3662	ENSSSCG00000011010.3	A0A287AZU9	C9orf72
10		LLPS-Sus-3660	ENSSSCG00000010311.3	A0A286ZKT1	CAMK2G
11		LLPS-Sus-1468	ENSSSCG00000017529.3	F1RWH1	CBX1
12		LLPS-Sus-0148	ENSSSCG00000024261.2	I3LU16	CBX2
13		LLPS-Sus-1648	ENSSSCG00000039191.1	F2Z5T3	CBX5
14		LLPS-Sus-1743	ENSSSCG00000038181.1	A0A286ZQB6	CCNT1
15		LLPS-Sus-2344	ENSSSCG00000016961.3	C9K505	CDK7
16		LLPS-Sus-0302	ENSSSCG00000005621.3	C9E1C9	CDK9
17		LLPS-Sus-3389	ENSSSCG00000013427.3	A0A286ZNG8	CIRBP
18		LLPS-Sus-1834	ENSSSCG00000038410.1	A0A288CFW3	CPEB2
19		LLPS-Sus-3261	ENSSSCG00000012486.4	I3LE23	CSTF2
20		LLPS-Sus-3070	ENSSSCG00000010430.3	F1SD01	CSTF2T
21		LLPS-Sus-1466	ENSSSCG00000031769.1	A0A287BKR0	CTTN
22		LLPS-Sus-4214	ENSSSCG00000005524.3	A0A287AFX0	DAB2IP
23		LLPS-Sus-0794	ENSSSCG00000001509.3	F1RZS8	DAXX
24		LLPS-Sus-3359	ENSSSCG00000022528.2	I3LB68	DAZAP1
25		LLPS-Sus-1163	ENSSSCG00000023976.2	I3LE95	DCP1B
26		LLPS-Sus-1492	ENSSSCG00000014204.3	A0A287BGM6	DCP2
27		LLPS-Sus-0243	ENSSSCG00000012252.3	F1RX16	DDX3X
28		LLPS-Sus-1579	ENSSSCG00000016913.3	F1SLL4	DDX4
29		LLPS-Sus-1007	ENSSSCG00000015097.3	F1SAJ5	DDX6
30		LLPS-Sus-2253	ENSSSCG00000024285.2	I3LMS7	DLG4
31		LLPS-Sus-1558	ENSSSCG00000003690.3	A0A287B2P3	DLGAP1
32		LLPS-Sus-4545	ENSSSCG00000030480.2	I3L9V1	DYRK1A
33		LLPS-Sus-1781	ENSSSCG00000001903.3	F1SIE7	EDC3
34		LLPS-Sus-2923	ENSSSCG00000003512.3	A0A287AF76	EIF4G3
35		LLPS-Sus-2624	ENSSSCG00000006099.3	A0A286ZV42	ESRP1
36		LLPS-Sus-3401	ENSSSCG00000009974.3	A0A287APW0	EWSR1
37		LLPS-Sus-2561	ENSSSCG00000017013.3	F1RR91	FBLL1
38		LLPS-Sus-1217	ENSSSCG00000033299.1	A0A287A4W0	FLNA
39		LLPS-Sus-3591	ENSSSCG00000011471.3	A0A287BLE0	FLNB
40		LLPS-Sus-2044	ENSSSCG00000012727.4	K7GSF4	FMR1
41		LLPS-Sus-0675	ENSSSCG00000030798.2	A0A287BPC6	FUS
42		LLPS-Sus-2865	ENSSSCG00000004421.4	A1Y2K1	FYN
43		LLPS-Sus-1588	ENSSSCG00000009137.3	I3LVQ6	GAR1
44		LLPS-Sus-0968	ENSSSCG00000031631.1	A0A287AKR9	GNL2
45		LLPS-Sus-0356	ENSSSCG00000017206.4	B6E241	GRB2
46		LLPS-Sus-3967	ENSSSCG00000021118.2	I3LNK5	GSPT1
47		LLPS-Sus-3475	ENSSSCG00000000288.3	A0A286ZPE3	HNRNPA1
48		LLPS-Sus-2441	ENSSSCG00000036350.1	A0A286ZI52	HNRNPA2B1
49		LLPS-Sus-0964	ENSSSCG00000009249.3	F1RVC9	HNRNPD
50		LLPS-Sus-0207	ENSSSCG00000037245.1	A0A287B2L4	HNRNPDL
51		LLPS-Sus-1517	ENSSSCG00000014022.3	A0A287BL05	HNRNPH1
52		LLPS-Sus-3361	ENSSSCG00000033161.1	A0A286ZWW2	HNRNPH2
53		LLPS-Sus-4296	ENSSSCG00000010238.3	F2Z5W4	HNRNPH3
54		LLPS-Sus-1309	ENSSSCG00000013919.3	F1S8D2	HOMER3
55		LLPS-Sus-1182	ENSSSCG00000009509.3	F1RP44	IPO5
56		LLPS-Sus-2852	ENSSSCG00000024614.2	A0A287A536	ITSN1
57		LLPS-Sus-2201	ENSSSCG00000029005.2	I3LS70	KPNA2
58		LLPS-Sus-0099	ENSSSCG00000017514.3	F1RWJ5	KPNB1
59		LLPS-Sus-1484	ENSSSCG00000007811.4	K7GT47	LAT
60		LLPS-Sus-1782	ENSSSCG00000032397.1	M3VH35	LBR
61		LLPS-Sus-2697	ENSSSCG00000005055.4	K7GRV5	LGALS3
62		LLPS-Sus-4541	ENSSSCG00000000194.3	F1SHC1	LOC100127131
63		LLPS-Sus-0811	ENSSSCG00000034314.1	A0A287AKQ5	LSM4
64		LLPS-Sus-0797	ENSSSCG00000000492.4	A0A2C9F343	LYZ
65		LLPS-Sus-3088	ENSSSCG00000007281.3	D2KQR0	MAP1LC3A
66		LLPS-Sus-1201	ENSSSCG00000002655.3	A0A287B8I0	MAP1LC3B
67		LLPS-Sus-4419	ENSSSCG00000017311.3	A0A286ZXV0	MAPT
68		LLPS-Sus-3985	ENSSSCG00000030211.2	A0A287A9L9	NBR1
69		LLPS-Sus-3015	ENSSSCG00000025701.2	A0A287BMS5	NCK1
70		LLPS-Sus-3133	ENSSSCG00000029284.2	A0A286ZZA9	NPHS1
71		LLPS-Sus-3091	ENSSSCG00000016998.3	F1RRY2	NPM1
72		LLPS-Sus-1347	ENSSSCG00000026034.2	I3LEL0	NUP153
73		LLPS-Sus-0036	ENSSSCG00000040333.1	A0A287AZ57	NUP62
74		LLPS-Sus-0082	ENSSSCG00000014794.3	F1SUZ2	NUP98
75		LLPS-Sus-1508	ENSSSCG00000006063.3	F2Z557	PABPC1
76		LLPS-Sus-3324	ENSSSCG00000001930.3	A0A287B8G0	PKM
77		LLPS-Sus-2279	ENSSSCG00000026748.2	I3LII4	PLK1
78		LLPS-Sus-4826	ENSSSCG00000013572.3	A0A286ZW95	PNPLA6
79		LLPS-Sus-0402	ENSSSCG00000028278.2	I3LJR4	POLR2A
80		LLPS-Sus-2025	ENSSSCG00000036145.1	Q28948	PRKAA2
81		LLPS-Sus-0060	ENSSSCG00000003189.3	F1RHU6	PRMT1
82		LLPS-Sus-0940	ENSSSCG00000009569.3	A0A286ZKW2	PSPC1
83		LLPS-Sus-1420	ENSSSCG00000013421.3	A0A286ZKQ3	PTBP1
84		LLPS-Sus-0868	ENSSSCG00000017475.3	F1RXC9	RARA
85		LLPS-Sus-3601	ENSSSCG00000007917.3	A0A287BS62	RBFOX1
86		LLPS-Sus-1096	ENSSSCG00000038008.1	A0A287B401	RBM14
87		LLPS-Sus-0740	ENSSSCG00000021591.2	I3L5X7	RBM3
88		LLPS-Sus-2094	ENSSSCG00000035080.1	A0A286ZL51	RPL23A
89		LLPS-Sus-1496	ENSSSCG00000006899.3	A0A287ASD0	RPL5
90		LLPS-Sus-3880	ENSSSCG00000011207.3	F1RS59	SGO1
91		LLPS-Sus-0698	ENSSSCG00000031925.1	A0A287BEU5	SHANK3
92		LLPS-Sus-0981	ENSSSCG00000008478.3	F1S4T0	SOS1
93		LLPS-Sus-0262	ENSSSCG00000014016.3	F1S445	SQSTM1
94		LLPS-Sus-0683	ENSSSCG00000032106.1	A0A287B433	SURF6
95		LLPS-Sus-2950	ENSSSCG00000012276.3	B7TY10	SYN1
96		LLPS-Sus-2435	ENSSSCG00000023225.2	A0A287ALW8	SYN2
97		LLPS-Sus-2925	ENSSSCG00000001513.3	A0A287BNM2	SYNGAP1
98		LLPS-Sus-2766	ENSSSCG00000017707.3	F1S187	TAF15
99		LLPS-Sus-0261	ENSSSCG00000023126.2	I3LNA4	TARDBP
100		LLPS-Sus-0370	ENSSSCG00000023409.2	A0A287A0R0	TIA1
101		LLPS-Sus-0029	ENSSSCG00000010685.3	F2Z5F9	TIAL1
102		LLPS-Sus-0168	ENSSSCG00000027293.2	I3LM93	TNPO1
103		LLPS-Sus-4322	ENSSSCG00000000078.3	A0A287BNM0	TNRC6B
104		LLPS-Sus-0826	ENSSSCG00000017950.3	Q9TUB2	TP53
105		LLPS-Sus-4069	ENSSSCG00000035264.1	A0A286ZWA5	UBQLN2
106		LLPS-Sus-3735	ENSSSCG00000013510.3	F1S7L6	UBXN6
107		LLPS-Sus-4017	ENSSSCG00000015843.3	F1RX67	UBXN8
108		LLPS-Sus-3558	ENSSSCG00000016604.3	F1SMK2	WASL
109		LLPS-Sus-0947	ENSSSCG00000028228.2	A0A287BHC9	XPO1
110		LLPS-Sus-3899	ENSSSCG00000017732.3	A0A287AD24	ZNF207