※ Droplet introduction

    Interactions between diverse synthetic, multivalent macromolecules (including multi-domain proteins and RNA) produce sharp liquid-liquid-demixing phase separations, generating micrometre-sized liquid droplets in aqueous solution (1-2). In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments. By now, various proteins have been purified and tested for their ability to phase separate and form liquid condensates in vitro (3).

References
1. Li P., Banjade S., Cheng H.C., et al. (2012) Phase transitions in the assembly of multivalent signalling proteins. Nature, 483, 336-40. PMID: 22398450
2. Kato M., Han T.W., Xie S., et al. (2012) Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Cell, 149, 753-67. PMID: 22579281
3. Alberti S., Saha S., Woodruff J.B., et al. (2018) A User’s Guide for Phase Separation Assays with Purified Proteins. J. Mol. Biol., 430, 4806-4820. PMID: 29944854

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No.	Status	DrLLPS ID	Ensemble Gene ID	UniProt Accession	Gene Name
1		LLPS-Sei-0297	SETIT_003252mg
2		LLPS-Sei-0184	SETIT_035065mg	K4A859	101752574
3		LLPS-Sei-0230	SETIT_030203mg	K3ZUC2	101752761
4		LLPS-Sei-0167	SETIT_021165mg	K3Z3P7	101754033
5		LLPS-Sei-0642	SETIT_028746mg	K3ZQ66	101754537
6		LLPS-Sei-1392	SETIT_002441mg	K3XKL8	101757314
7		LLPS-Sei-2361	SETIT_022780mg	K3Z8B0	101760788
8		LLPS-Sei-0192	SETIT_017895mg	K3YUE9	101762213
9		LLPS-Sei-1100	SETIT_034064mg	K4A5B0	101763385
10		LLPS-Sei-0697	SETIT_002374mg	K3XKF1	101764495
11		LLPS-Sei-1381	SETIT_009571mg	K3Y5T2	101769033
12		LLPS-Sei-0070	SETIT_030149mg	K3ZU68	101773168
13		LLPS-Sei-0064	SETIT_017805mg	K3YU59	101774232
14		LLPS-Sei-1345	SETIT_030356mg	K3ZUS5	101776815
15		LLPS-Sei-2046	SETIT_025940mg	K3ZH88	101776833
16		LLPS-Sei-0756	SETIT_024149mg	K3ZC75	101780106
17		LLPS-Sei-0446	SETIT_011236mg	K3YAJ8	101781149
18		LLPS-Sei-1689	SETIT_022140mg	K3Z6H1	101781985
19		LLPS-Sei-1476	SETIT_039110mg	K4AJN9	101782013
20		LLPS-Sei-0010	SETIT_026888mg	K3ZJY4	101783998
21		LLPS-Sei-0345	SETIT_014096mg	K3YIM7	101784763
22		LLPS-Sei-0689	SETIT_023101mg	K3Z980	101785067
23		LLPS-Sei-1440	SETIT_016927mg	K3YRN3	101786376
24		LLPS-Sei-0185	SETIT_016856mg	K3YRG2	101786486
25		LLPS-Sei-1690	SETIT_016687mg	K3YQZ3	101786488
26		LLPS-Sei-0668	SETIT_028775mg	A0A368PXU9	SETIT_2G111900v2
27		LLPS-Sei-1801	SETIT_029883mg	A0A368Q148	SETIT_2G200500v2
28		LLPS-Sei-1203	SETIT_032662mg	A0A368Q795	SETIT_2G381300v2
29		LLPS-Sei-0362	SETIT_000965mg	A0A368RAH6	SETIT_5G305000v2
30		LLPS-Sei-0472	SETIT_000473mg	A0A368RDR3	SETIT_5G387300v2
31		LLPS-Sei-0144	SETIT_013525mg	A0A368RNS4	SETIT_6G212200v2
32		LLPS-Sei-0291	SETIT_012624mg	A0A368RXF6	SETIT_7G185500v2
33		LLPS-Sei-1316	SETIT_009305mg	A0A368S2Q7	SETIT_7G335200v2
34		LLPS-Sei-2194	SETIT_027602mg	A0A368S4I0	SETIT_8G054300v2
35		LLPS-Sei-2123	SETIT_027380mg	A0A368S4Y5	SETIT_8G067000v2
36		LLPS-Sei-0722	SETIT_028000mg	A0A368S7N6	SETIT_8G135500v2
37		LLPS-Sei-1018	SETIT_026655mg	A0A368S9L5	SETIT_8G198900v2
38		LLPS-Sei-1278	SETIT_036395mg	A0A368SLD7	SETIT_9G278400v2