※ Droplet introduction

    Interactions between diverse synthetic, multivalent macromolecules (including multi-domain proteins and RNA) produce sharp liquid-liquid-demixing phase separations, generating micrometre-sized liquid droplets in aqueous solution (1-2). In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments. By now, various proteins have been purified and tested for their ability to phase separate and form liquid condensates in vitro (3).

References
1. Li P., Banjade S., Cheng H.C., et al. (2012) Phase transitions in the assembly of multivalent signalling proteins. Nature, 483, 336-40. PMID: 22398450
2. Kato M., Han T.W., Xie S., et al. (2012) Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Cell, 149, 753-67. PMID: 22579281
3. Alberti S., Saha S., Woodruff J.B., et al. (2018) A User’s Guide for Phase Separation Assays with Purified Proteins. J. Mol. Biol., 430, 4806-4820. PMID: 29944854

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No.	Status	DrLLPS ID	Ensemble Gene ID	UniProt Accession	Gene Name
1		LLPS-Sem-0012	SELMODRAFT_100958	D8RT90	CDKD-1
2		LLPS-Sem-1399	SELMODRAFT_449602	D8RQ46	FK-1
3		LLPS-Sem-0743	SELMODRAFT_128761	D8SZU3	LSM4
4		LLPS-Sem-0907	SELMODRAFT_11479	D8R8N6	SELMODRAFT_11479
5		LLPS-Sem-2161	SELMODRAFT_115313	D8SF78	SELMODRAFT_115313
6		LLPS-Sem-0985	SELMODRAFT_125897	D8SVA8	SELMODRAFT_125897
7		LLPS-Sem-0872	SELMODRAFT_128226	D8SYZ7	SELMODRAFT_128226
8		LLPS-Sem-1277	SELMODRAFT_134716	D8T929	SELMODRAFT_134716
9		LLPS-Sem-0433	SELMODRAFT_140234	D8QP56	SELMODRAFT_140234
10		LLPS-Sem-0286	SELMODRAFT_140819	D8QSR9	SELMODRAFT_140819
11		LLPS-Sem-1284	SELMODRAFT_144528	D8R7T5	SELMODRAFT_144528
12		LLPS-Sem-2012	SELMODRAFT_154687	D8SE79	SELMODRAFT_154687
13		LLPS-Sem-1439	SELMODRAFT_155165	D8SGN2	SELMODRAFT_155165
14		LLPS-Sem-0846	SELMODRAFT_158150	D8STL4	SELMODRAFT_158150
15		LLPS-Sem-0119	SELMODRAFT_158648	D8SVC8	SELMODRAFT_158648
16		LLPS-Sem-0513	SELMODRAFT_171051	D8RFP8	SELMODRAFT_171051
17		LLPS-Sem-1088	SELMODRAFT_175519	D8RYY9	SELMODRAFT_175519
18		LLPS-Sem-0429	SELMODRAFT_178975	D8SE24	SELMODRAFT_178975
19		LLPS-Sem-1492	SELMODRAFT_233860	D8SF68	SELMODRAFT_233860
20		LLPS-Sem-1282	SELMODRAFT_233936	D8SFW6	SELMODRAFT_233936
21		LLPS-Sem-1041	SELMODRAFT_236158	D8T5P3	SELMODRAFT_236158
22		LLPS-Sem-0263	SELMODRAFT_266974	D8R5G8	SELMODRAFT_266974
23		LLPS-Sem-1508	SELMODRAFT_271663	D8SKB2	SELMODRAFT_271663
24		LLPS-Sem-2011	SELMODRAFT_28301	D8T893	SELMODRAFT_28301
25		LLPS-Sem-0430	SELMODRAFT_28398	D8SV82	SELMODRAFT_28398
26		LLPS-Sem-1709	SELMODRAFT_411870	D8RJA7	SELMODRAFT_411870
27		LLPS-Sem-2180	SELMODRAFT_42150	D8T5S0	SELMODRAFT_42150
28		LLPS-Sem-1570	SELMODRAFT_438877	D8R034	SELMODRAFT_438877
29		LLPS-Sem-2390	SELMODRAFT_441992	D8RPI5	SELMODRAFT_441992
30		LLPS-Sem-0075	SELMODRAFT_443985	D8S617	SELMODRAFT_443985
31		LLPS-Sem-0919	SELMODRAFT_44478	D8RFQ2	SELMODRAFT_44478
32		LLPS-Sem-0604	SELMODRAFT_446169	D8SP98	SELMODRAFT_446169
33		LLPS-Sem-1324	SELMODRAFT_74731	D8QMI1	SELMODRAFT_74731
34		LLPS-Sem-0954	SELMODRAFT_8465	D8RNS7	SELMODRAFT_8465
35		LLPS-Sem-0026	SELMODRAFT_450254	D8SY37	SNRK1.2-1
36		LLPS-Sem-1415	SELMODRAFT_449361	D8TFM6	TOR-2