※ Droplet introduction

    Interactions between diverse synthetic, multivalent macromolecules (including multi-domain proteins and RNA) produce sharp liquid-liquid-demixing phase separations, generating micrometre-sized liquid droplets in aqueous solution (1-2). In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments. By now, various proteins have been purified and tested for their ability to phase separate and form liquid condensates in vitro (3).

References
1. Li P., Banjade S., Cheng H.C., et al. (2012) Phase transitions in the assembly of multivalent signalling proteins. Nature, 483, 336-40. PMID: 22398450
2. Kato M., Han T.W., Xie S., et al. (2012) Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Cell, 149, 753-67. PMID: 22579281
3. Alberti S., Saha S., Woodruff J.B., et al. (2018) A User’s Guide for Phase Separation Assays with Purified Proteins. J. Mol. Biol., 430, 4806-4820. PMID: 29944854

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No.	Status	DrLLPS ID	Ensemble Gene ID	UniProt Accession	Gene Name
1		LLPS-Mae-0753	MANES_05G063400	A0A2C9VW93	LSM4
2		LLPS-Mae-0894	MANES_01G017000	A0A2C9WJF0	MANES_01G017000
3		LLPS-Mae-0157	MANES_01G151000	A0A251M2B5	MANES_01G151000
4		LLPS-Mae-1059	MANES_01G155200	A0A2C9WKZ6	MANES_01G155200
5		LLPS-Mae-2498	MANES_02G049300	A0A2C9WAX4	MANES_02G049300
6		LLPS-Mae-1342	MANES_03G031500	A0A2C9W6L6	MANES_03G031500
7		LLPS-Mae-1164	MANES_03G075400	A0A2C9W7K1	MANES_03G075400
8		LLPS-Mae-1295	MANES_03G112600	A0A2C9W6F8	MANES_03G112600
9		LLPS-Mae-0470	MANES_03G144500	A0A2C9W7E9	MANES_03G144500
10		LLPS-Mae-1380	MANES_04G104700	A0A2C9W1B9	MANES_04G104700
11		LLPS-Mae-0059	MANES_04G142700	A0A2C9W2D8	MANES_04G142700
12		LLPS-Mae-0337	MANES_05G037200	A0A2C9VT15	MANES_05G037200
13		LLPS-Mae-2256	MANES_05G143000	A0A2C9VW54	MANES_05G143000
14		LLPS-Mae-0795	MANES_06G009200	A0A2C9VLP0	MANES_06G009200
15		LLPS-Mae-0476	MANES_06G023400	A0A2C9VM73	MANES_06G023400
16		LLPS-Mae-2383	MANES_06G174000	A0A251KRJ6	MANES_06G174000
17		LLPS-Mae-1818	MANES_07G056600	A0A251JZB7	MANES_07G056600
18		LLPS-Mae-1489	MANES_07G100300	A0A2C9VLN6	MANES_07G100300
19		LLPS-Mae-0356	MANES_07G111000	A0A251KIT5	MANES_07G111000
20		LLPS-Mae-0364	MANES_08G095300	A0A2C9VGT3	MANES_08G095300
21		LLPS-Mae-0440	MANES_08G144000	A0A2C9VHW2	MANES_08G144000
22		LLPS-Mae-0884	MANES_08G163100	A0A2C9VJ81	MANES_08G163100
23		LLPS-Mae-2325	MANES_09G051800	A0A2C9V812	MANES_09G051800
24		LLPS-Mae-1805	MANES_11G016600	A0A2C9UX41	MANES_11G016600
25		LLPS-Mae-1823	MANES_13G016600	A0A2C9UN06	MANES_13G016600
26		LLPS-Mae-0690	MANES_13G120600	A0A2C9US98	MANES_13G120600
27		LLPS-Mae-0321	MANES_14G023700	A0A2C9UJ35	MANES_14G023700
28		LLPS-Mae-1337	MANES_14G168200	A0A2C9UMI7	MANES_14G168200
29		LLPS-Mae-0515	MANES_15G106400	A0A251J4V2	MANES_15G106400
30		LLPS-Mae-1734	MANES_15G108300	A0A2C9UGS7	MANES_15G108300
31		LLPS-Mae-0036	MANES_15G133200	A0A2C9UFU0	MANES_15G133200
32		LLPS-Mae-0295	MANES_15G145000	A0A2C9UG38	MANES_15G145000
33		LLPS-Mae-0812	MANES_15G163200	A0A2C9UGS2	MANES_15G163200
34		LLPS-Mae-2279	MANES_16G035000	A0A251IY90	MANES_16G035000
35		LLPS-Mae-0322	MANES_16G076400	A0A2C9U9X1	MANES_16G076400
36		LLPS-Mae-1326	MANES_16G093200	A0A2C9U9Y7	MANES_16G093200
37		LLPS-Mae-0678	MANES_18G093400	A0A2C9U1T2	MANES_18G093400
38		LLPS-Mae-0412	MANES_18G146300	A0A251ISY3	MANES_18G146300