※ Droplet introduction

    Interactions between diverse synthetic, multivalent macromolecules (including multi-domain proteins and RNA) produce sharp liquid-liquid-demixing phase separations, generating micrometre-sized liquid droplets in aqueous solution (1-2). In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments. By now, various proteins have been purified and tested for their ability to phase separate and form liquid condensates in vitro (3).

References
1. Li P., Banjade S., Cheng H.C., et al. (2012) Phase transitions in the assembly of multivalent signalling proteins. Nature, 483, 336-40. PMID: 22398450
2. Kato M., Han T.W., Xie S., et al. (2012) Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Cell, 149, 753-67. PMID: 22579281
3. Alberti S., Saha S., Woodruff J.B., et al. (2018) A User’s Guide for Phase Separation Assays with Purified Proteins. J. Mol. Biol., 430, 4806-4820. PMID: 29944854

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No.	Status	DrLLPS ID	Ensemble Gene ID	UniProt Accession	Gene Name
1		LLPS-Glm-2276	GLYMA_04G087100	A0A0R0KCT9	100775451
2		LLPS-Glm-1930	GLYMA_16G005700	K7MEE8	100778053
3		LLPS-Glm-1363	GLYMA_05G041600	K7KMT5	100778692
4		LLPS-Glm-1081	GLYMA_19G196300	I1NAR0	100780081
5		LLPS-Glm-1402	GLYMA_07G215300	I1KM25	100780329
6		LLPS-Glm-0192	GLYMA_12G028800	K7LSR9	100780819
7		LLPS-Glm-1630	GLYMA_13G278500	I1M3A3	100781028
8		LLPS-Glm-2754	GLYMA_20G136000	I1NG45	100781185
9		LLPS-Glm-1745	GLYMA_05G155100	A0A0R0JVZ4	100781743
10		LLPS-Glm-0156	GLYMA_07G128100	I1KJU3	100781777
11		LLPS-Glm-0862	GLYMA_05G020900	I1JZI4	100782808
12		LLPS-Glm-2073	GLYMA_05G243400	A0A0R0K0Z8	100784412
13		LLPS-Glm-2909	GLYMA_04G200700	I1MYV4	100789137
14		LLPS-Glm-2254	GLYMA_12G235000	I1LVB2	100789177
15		LLPS-Glm-1627	GLYMA_05G046100	C6TBP8	100790268
16		LLPS-Glm-0996	GLYMA_14G005800	I1M679	100790967
17		LLPS-Glm-1712	GLYMA_20G106300	I1NF81	100792702
18		LLPS-Glm-2082	GLYMA_18G262900	I1N4G7	100796687
19		LLPS-Glm-1219	GLYMA_16G041400	I1ML28	100799823
20		LLPS-Glm-0965	GLYMA_07G072900	I1KID2	100801402
21		LLPS-Glm-0596	GLYMA_11G092200	I1LII4	100802384
22		LLPS-Glm-1163	GLYMA_13G112100	I1LY79	100803322
23		LLPS-Glm-2419	GLYMA_07G223700	K7L395	100803349
24		LLPS-Glm-1222	GLYMA_08G114900	I1KSC8	100805849
25		LLPS-Glm-2246	GLYMA_20G172200	I1NH73	100806489
26		LLPS-Glm-2930	GLYMA_05G235400	I1K5Q4	100807576
27		LLPS-Glm-1005	GLYMA_07G224800	I1KMC2	100807615
28		LLPS-Glm-0305	GLYMA_02G074100	C6TF29	100807659
29		LLPS-Glm-2060	GLYMA_12G048600	I1LQ96	100813285
30		LLPS-Glm-0826	GLYMA_09G198500	I1L4R0	100816391
31		LLPS-Glm-0605	GLYMA_07G059900	I1KHZ4	100817746
32		LLPS-Glm-1105	GLYMA_08G025600	I1KPN2	100818831
33		LLPS-Glm-2802	GLYMA_08G049300	I1KQF1	100819009
34		LLPS-Glm-0520	GLYMA_04G076700	K7KIR9	GLYMA_04G076700
35		LLPS-Glm-1202	GLYMA_08G050900	I1KQG7	GLYMA_08G050900
36		LLPS-Glm-0229	GLYMA_08G223200	K7L852	GLYMA_08G223200
37		LLPS-Glm-0394	GLYMA_09G278900	I1L748	GLYMA_09G278900
38		LLPS-Glm-0988	GLYMA_13G097100	I1LVQ6	GLYMA_13G097100
39		LLPS-Glm-0351	GLYMA_13G332700	I1M4S4	GLYMA_13G332700
40		LLPS-Glm-2616	GLYMA_02G155300	A0A0R4J2Q5	LSM4