※ Droplet introduction

    Interactions between diverse synthetic, multivalent macromolecules (including multi-domain proteins and RNA) produce sharp liquid-liquid-demixing phase separations, generating micrometre-sized liquid droplets in aqueous solution (1-2). In vitro phase separation assays with purified proteins have become the standard way to investigate proteins that form membrane-less compartments. By now, various proteins have been purified and tested for their ability to phase separate and form liquid condensates in vitro (3).

References
1. Li P., Banjade S., Cheng H.C., et al. (2012) Phase transitions in the assembly of multivalent signalling proteins. Nature, 483, 336-40. PMID: 22398450
2. Kato M., Han T.W., Xie S., et al. (2012) Cell-free formation of RNA granules: low complexity sequence domains form dynamic fibers within hydrogels. Cell, 149, 753-67. PMID: 22579281
3. Alberti S., Saha S., Woodruff J.B., et al. (2018) A User’s Guide for Phase Separation Assays with Purified Proteins. J. Mol. Biol., 430, 4806-4820. PMID: 29944854

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No.	Status	DrLLPS ID	Ensemble Gene ID	UniProt Accession	Gene Name
1		LLPS-Ast-0054	ATEG_10203	Q0C7Y1	adk1
2		LLPS-Ast-0161	ATEG_00481	Q0D0Q3	ATEG_00481
3		LLPS-Ast-0163	ATEG_00625	Q0D0A9	ATEG_00625
4		LLPS-Ast-0778	ATEG_00680	Q0D054	ATEG_00680
5		LLPS-Ast-0936	ATEG_02027	Q0CWA7	ATEG_02027
6		LLPS-Ast-0060	ATEG_02848	Q0CTY6	ATEG_02848
7		LLPS-Ast-0583	ATEG_02938	Q0CTP6	ATEG_02938
8		LLPS-Ast-0294	ATEG_03054	Q0CTD0	ATEG_03054
9		LLPS-Ast-0417	ATEG_03250	Q0CST4	ATEG_03250
10		LLPS-Ast-0536	ATEG_03284	Q0CSQ0	ATEG_03284
11		LLPS-Ast-0020	ATEG_03685	Q0CRJ9	ATEG_03685
12		LLPS-Ast-1494	ATEG_03838	Q0CR46	ATEG_03838
13		LLPS-Ast-1032	ATEG_03971	Q0CQR3	ATEG_03971
14		LLPS-Ast-0032	ATEG_04232	Q0CQ02	ATEG_04232
15		LLPS-Ast-0236	ATEG_04386	Q0CPJ8	ATEG_04386
16		LLPS-Ast-1255	ATEG_05191	Q0CM93	ATEG_05191
17		LLPS-Ast-0125	ATEG_05200	Q0CM84	ATEG_05200
18		LLPS-Ast-0028	ATEG_05222	Q0CM62	ATEG_05222
19		LLPS-Ast-0378	ATEG_05322	Q0CLW2	ATEG_05322
20		LLPS-Ast-0668	ATEG_06018	Q0CJW6	ATEG_06018
21		LLPS-Ast-0869	ATEG_06171	Q0CJG3	ATEG_06171
22		LLPS-Ast-0170	ATEG_06864	Q0CHH8	ATEG_06864
23		LLPS-Ast-1263	ATEG_07632	Q0CFA2	ATEG_07632
24		LLPS-Ast-0763	ATEG_07853	Q0CEN1	ATEG_07853
25		LLPS-Ast-0387	ATEG_08222	Q0CDL2	ATEG_08222
26		LLPS-Ast-0169	ATEG_08266	Q0CDG8	ATEG_08266
27		LLPS-Ast-1168	ATEG_08341	Q0CD93	ATEG_08341
28		LLPS-Ast-0207	ATEG_08495	Q0CCT9	ATEG_08495
29		LLPS-Ast-0474	ATEG_08958	Q0CBH6	ATEG_08958
30		LLPS-Ast-0464	ATEG_09467	Q0CA17	ATEG_09467
31		LLPS-Ast-0128	ATEG_09560	Q0C9S4	ATEG_09560
32		LLPS-Ast-0981	ATEG_08993	Q0CBE1	dhh1
33		LLPS-Ast-0107	ATEG_01137	Q0CYU7	LSM4
34		LLPS-Ast-1496	ATEG_04633	Q0CNV1	pdcA