DrLLPS - Data resource of LLPS

LLPS-Tut-1167
NCBP2

Integrated Annotations

▼ OVERVIEW


Status:	Unreviewed
Protein Name:	Nuclear cap-binding protein subunit 2; 20 kDa nuclear cap-binding protein
Gene Name:	NCBP2
Ensembl Gene:	ENSTTRG00000013908.1
Ensembl Protein:	ENSTTRP00000013185.1
Organism:	Tursiops truncatus
Taxa ID:	9739
LLPS Type:	LLPS regulator

▼ PROPERTY

——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:

Condensate	Evidence	Orthologs
Nuclear speckle, Nucleolus, P-body	Predicted from orthologs	(View)

Organism	DrLLPS ID	UniProt ID	LLPS type	Condensate	Identity	E-value	Score
Caenorhabditis elegans	LLPS-Cae-0162	Q93594	Regulator	P-body	64.58	1e-64	201
Arabidopsis thaliana	LLPS-Art-0215	Q9XFD1	Client	Nucleolus	67.86	2e-51	172
Mus musculus	LLPS-Mum-0995	Q9CQ49	Client	Nuclear speckle	98.35	1e-81	245

▼ FUNCTION

Component of the cap-binding complex (CBC), which binds co-transcriptionally to the 5' cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus, leading to the recruitment of the mRNA export machinery to the 5' end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC), promoting the interaction between upf1 and upf2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with srrt/ars2, thereby being required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of parn, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, ncbp2/cbp20 recognizes and binds capped RNAs (m7GpppG-capped RNA) but requires ncbp1/cbp80 to stabilize the movement of its N-terminal loop and lock the CBC into a high affinity cap-binding state with the cap structure. The conventional cap-binding complex with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus.

▼ CROSS REFERENCE

Database	Nucleotide ID	Protein ID
Ensembl	ENSTTRT00000013904.1	ENSTTRP00000013185.1
UniProt		A0A2U3V5D3, A0A2U3V5D3_TURTR
RefSeq	, XM_004321608.2	, XP_004321656.2, XP_004321656.2

▼ SEQUENCE

Protein Sequence (FASTA)
1 MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF 60 61 SKSGDIKKII MGLDKMKKTA CGFCFVEYYL RADAENAMRY INGTRLDDRI IRTDWDAGFK 120 121 EGRQYGRGRS GGQVRDEYRQ DYDAGRGGYG KLAQNL 156
Nucleotide CDS Sequence (FASTA)
1 ATGTCGGGTG GCCTCCTGAA GGCGCTGCGC AGCGACTCCT ACGTCGAGCT GAGCCAGTAC 60 61 CGGGACCAGC ACTTCCGGGG TGACAATGAA GAACAGGAAA AATTGCTGAA GAAAAGCTGT 120 121 ACATTGTATG TTGGAAATCT TTCCTTTTAT ACAACTGAAG AACAAATCTA TGAACTCTTC 180 181 AGCAAAAGTG GTGACATAAA GAAAATCATC ATGGGCCTGG ATAAAATGAA GAAAACAGCA 240 241 TGTGGGTTCT GCTTTGTGGA ATACTATTTA AGAGCAGATG CAGAAAATGC CATGCGGTAC 300 301 ATAAATGGAA CTCGTCTGGA TGACCGGATC ATTCGCACAG ACTGGGACGC AGGCTTTAAG 360 361 GAGGGCAGGC AGTATGGCCG TGGGCGTTCT GGGGGCCAGG TACGAGATGA GTATCGTCAG 420 421 GACTATGATG CTGGGAGAGG AGGCTATGGA AAACTGGCCC AAAACCTGTG A 471

▼ KEYWORD

ID	Family
KW-0181	Complete proteome
KW-0507	mRNA processing
KW-0508	mRNA splicing
KW-0539	Nucleus
KW-1185	Reference proteome
KW-0694	RNA-binding

▼ GENE ONTOLOGY

ID	Classification	Description
GO:0005846	Cellular Component	Nuclear cap binding complex
GO:0005634	Cellular Component	Nucleus
GO:0000339	Molecular Function	RNA cap binding
GO:0045292	Biological Process	MRNA cis splicing, via spliceosome

▼ ORTHOLOGY

DrLLPS ID	Organism	Identity	E-value	Score
LLPS-Mal-2649	Mandrillus leucophaeus	100.0	1e-24	97.1
LLPS-Paa-1495	Papio anubis	100.0	1e-24	97.1
LLPS-Ova-1722	Ovis aries	99.35	1e-95	280
LLPS-Aim-0173	Ailuropoda melanoleuca	99.35	1e-95	280
LLPS-Mup-0342	Mustela putorius furo	99.35	2e-95	280
LLPS-Bot-0794	Bos taurus	99.35	1e-95	280
LLPS-Caj-1975	Callithrix jacchus	99.35	1e-95	280
LLPS-Maf-1843	Macaca fascicularis	99.35	1e-95	280
LLPS-Chs-1299	Chlorocebus sabaeus	99.35	1e-95	283
LLPS-Sus-0588	Sus scrofa	99.35	1e-95	280
LLPS-Aon-3563	Aotus nancymaae	99.35	1e-95	280
LLPS-Cea-2593	Cercocebus atys	99.35	1e-95	280
LLPS-Caf-2700	Canis familiaris	99.35	1e-95	280
LLPS-Poa-1440	Pongo abelii	99.35	2e-95	281
LLPS-Eqc-1456	Equus caballus	99.35	1e-95	280
LLPS-Mam-0605	Macaca mulatta	99.35	1e-95	283
LLPS-Otg-3097	Otolemur garnettii	99.35	1e-95	280
LLPS-Hos-0057	Homo sapiens	99.35	1e-95	280
LLPS-Urm-2377	Ursus maritimus	99.35	1e-95	280
LLPS-Fec-1137	Felis catus	99.35	1e-95	280
LLPS-Man-1691	Macaca nemestrina	99.35	1e-95	280
LLPS-Myl-2312	Myotis lucifugus	98.71	8e-95	278
LLPS-Cas-0356	Carlito syrichta	98.71	7e-95	278
LLPS-Orc-0112	Oryctolagus cuniculus	98.71	7e-95	278
LLPS-Loa-0908	Loxodonta africana	98.71	4e-95	279
LLPS-Mum-0995	Mus musculus	98.35	2e-81	244
LLPS-Ran-0192	Rattus norvegicus	98.05	2e-93	275
LLPS-Fud-1126	Fukomys damarensis	98.05	2e-93	275
LLPS-Mea-0745	Mesocricetus auratus	98.05	2e-93	275
LLPS-Cap-1626	Cavia porcellus	98.05	9e-94	275
LLPS-Gog-2133	Gorilla gorilla	97.71	1e-75	229
LLPS-Pap-0890	Pan paniscus	97.71	1e-75	229
LLPS-Dio-0587	Dipodomys ordii	97.42	2e-93	275
LLPS-Ict-0748	Ictidomys tridecemlineatus	94.41	2e-91	270
LLPS-Nol-0865	Nomascus leucogenys	93.33	2e-92	273
LLPS-Pat-0854	Pan troglodytes	92.9	8e-84	250
LLPS-Rhb-0222	Rhinopithecus bieti	92.73	3e-92	272
LLPS-Sah-0239	Sarcophilus harrisii	91.61	7e-87	258
LLPS-Mod-1583	Monodelphis domestica	91.61	7e-87	258
LLPS-Pes-2464	Pelodiscus sinensis	90.77	6e-69	211
LLPS-Anp-1813	Anas platyrhynchos	89.15	2e-66	205
LLPS-Gaga-0503	Gallus gallus	88.89	2e-82	246
LLPS-Tag-0824	Taeniopygia guttata	88.16	1e-81	244
LLPS-Fia-1808	Ficedula albicollis	87.58	2e-81	244
LLPS-Xet-1066	Xenopus tropicalis	86.09	8e-81	243
LLPS-Leo-1435	Lepisosteus oculatus	85.91	3e-79	239
LLPS-Lac-1185	Latimeria chalumnae	85.53	9e-82	245
LLPS-Scf-0663	Scleropages formosus	84.56	9e-78	236
LLPS-Xim-1902	Xiphophorus maculatus	83.89	4e-77	233
LLPS-Asm-1920	Astyanax mexicanus	83.89	3e-77	234
LLPS-Orn-3112	Oreochromis niloticus	83.89	3e-78	236
LLPS-Pof-3137	Poecilia formosa	83.89	7e-78	235
LLPS-Scm-1577	Scophthalmus maximus	83.78	2e-76	231
LLPS-Tar-0372	Takifugu rubripes	83.33	2e-77	234
LLPS-Orl-1046	Oryzias latipes	83.22	3e-77	234
LLPS-Ten-0820	Tetraodon nigroviridis	83.22	1e-76	232
LLPS-Dar-1308	Danio rerio	82.55	2e-76	231
LLPS-Gaa-0147	Gasterosteus aculeatus	81.21	4e-75	228
LLPS-Meg-0977	Meleagris gallopavo	79.84	2e-60	190
LLPS-Drm-1377	Drosophila melanogaster	78.26	3e-66	206
LLPS-Anc-2097	Anolis carolinensis	75.84	3e-69	213
LLPS-Mua-0751	Musa acuminata	74.49	2e-34	122
LLPS-Cii-0326	Ciona intestinalis	73.15	2e-65	204
LLPS-Asfu-0083	Aspergillus fumigatus	72.41	6e-45	152
LLPS-Nef-0529	Neosartorya fischeri	72.41	2e-44	151
LLPS-Nec-0124	Neurospora crassa	72.27	4e-46	155
LLPS-Trr-0075	Trichoderma reesei	71.9	2e-46	155
LLPS-Prp-0811	Prunus persica	71.74	2e-53	176
LLPS-Asni-0223	Aspergillus niger	71.55	2e-44	150
LLPS-Mao-0170	Magnaporthe oryzae	71.43	2e-45	153
LLPS-Cis-0169	Ciona savignyi	71.33	1e-65	205
LLPS-Ori-0914	Oryza indica	71.3	3e-41	140
LLPS-Tra-2558	Triticum aestivum	71.22	7e-52	171
LLPS-Zem-0150	Zea mays	71.22	1e-53	176
LLPS-Sob-1236	Sorghum bicolor	71.22	4e-53	174
LLPS-Asc-0561	Aspergillus clavatus	71.15	2e-38	134
LLPS-Ora-0034	Ornithorhynchus anatinus	71.08	7e-35	124
LLPS-Trv-0399	Trichoderma virens	70.83	3e-45	152
LLPS-Mae-1382	Manihot esculenta	70.71	4e-54	177
LLPS-Gor-0509	Gossypium raimondii	70.71	2e-52	175
LLPS-Asf-0618	Aspergillus flavus	70.69	6e-45	152
LLPS-Tru-1127	Triticum urartu	70.54	3e-46	154
LLPS-Hov-1134	Hordeum vulgare	70.5	2e-51	171
LLPS-Brd-1345	Brachypodium distachyon	70.5	2e-51	171
LLPS-Glm-2677	Glycine max	70.5	7e-52	172
LLPS-Met-0493	Medicago truncatula	70.0	2e-52	173
LLPS-Ved-0829	Verticillium dahliae	70.0	4e-46	155
LLPS-Phn-0599	Phaeosphaeria nodorum	69.84	5e-47	156
LLPS-Orbr-0583	Oryza brachyantha	69.78	3e-52	172
LLPS-Mel-1091	Melampsora laricipopulina	69.35	4e-44	150
LLPS-Org-1010	Oryza glaberrima	69.29	2e-52	173
LLPS-Ors-1330	Oryza sativa	69.29	2e-52	173
LLPS-Lem-0043	Leptosphaeria maculans	69.11	1e-45	154
LLPS-Cogr-0024	Colletotrichum graminicola	69.11	2e-45	154
LLPS-Phv-1185	Phaseolus vulgaris	69.06	1e-50	169
LLPS-Viv-0300	Vitis vinifera	68.99	5e-46	153
LLPS-Ast-0124	Aspergillus terreus	68.48	1e-29	110
LLPS-Orb-0591	Oryza barthii	68.46	4e-45	154
LLPS-Coc-0130	Corchorus capsularis	68.46	5e-44	152
LLPS-Pyt-0021	Pyrenophora teres	68.25	2e-46	155
LLPS-Pytr-0461	Pyrenophora triticirepentis	68.25	2e-46	155
LLPS-Vir-0307	Vigna radiata	68.22	2e-45	152
LLPS-Dac-0304	Daucus carota	68.09	4e-49	165
LLPS-Arl-1128	Arabidopsis lyrata	67.86	1e-50	169
LLPS-Art-0215	Arabidopsis thaliana	67.86	1e-50	169
LLPS-Pot-0800	Populus trichocarpa	67.86	2e-52	173
LLPS-Tum-0904	Tuber melanosporum	67.8	8e-41	140
LLPS-Bro-0100	Brassica oleracea	67.69	1e-44	154
LLPS-Amt-0397	Amborella trichopoda	67.44	5e-45	152
LLPS-Scj-0264	Schizosaccharomyces japonicus	67.24	2e-42	145
LLPS-Fuv-0331	Fusarium verticillioides	67.2	3e-45	152
LLPS-Pug-0646	Puccinia graminis	67.2	5e-43	147
LLPS-Beb-0084	Beauveria bassiana	67.19	6e-46	154
LLPS-Sol-0004	Solanum lycopersicum	66.9	2e-49	166
LLPS-Coo-0460	Colletotrichum orbiculare	66.67	1e-45	153
LLPS-Yal-0428	Yarrowia lipolytica	66.39	6e-41	141
LLPS-Abg-0128	Absidia glauca	66.19	2e-50	167
LLPS-Blg-0011	Blumeria graminis	66.18	2e-45	153
LLPS-Dos-0575	Dothistroma septosporum	66.15	1e-43	149
LLPS-Sei-0464	Setaria italica	66.04	4e-43	150
LLPS-Php-0167	Physcomitrella patens	65.96	6e-45	154
LLPS-Brr-1002	Brassica rapa	65.28	1e-46	159
LLPS-Gag-1118	Gaeumannomyces graminis	65.19	2e-45	153
LLPS-Orgl-0582	Oryza glumaepatula	65.09	9e-40	147
LLPS-Lep-1258	Leersia perrieri	65.09	7e-43	147
LLPS-Orni-1726	Oryza nivara	65.09	2e-40	148
LLPS-Orr-1541	Oryza rufipogon	65.09	3e-40	148
LLPS-Orm-1365	Oryza meridionalis	65.09	1e-39	147
LLPS-Orp-0343	Oryza punctata	65.09	1e-42	147
LLPS-Cus-0000	Cucumis sativus	65.07	5e-47	159
LLPS-Gas-0477	Galdieria sulphuraria	65.0	5e-51	168
LLPS-Fus-0234	Fusarium solani	64.66	2e-44	150
LLPS-Cae-0162	Caenorhabditis elegans	64.58	5e-53	172
LLPS-Thc-0141	Theobroma cacao	64.49	3e-41	145
LLPS-Sem-0030	Selaginella moellendorffii	64.34	7e-43	145
LLPS-Hea-0728	Helianthus annuus	63.77	6e-43	149
LLPS-Brn-1197	Brassica napus	63.33	1e-46	159
LLPS-Spr-0841	Sporisorium reilianum	63.31	2e-43	150
LLPS-Cog-0546	Colletotrichum gloeosporioides	63.04	2e-46	155
LLPS-Chc-0022	Chondrus crispus	62.86	6e-47	159
LLPS-Sac-1308	Saccharomyces cerevisiae	62.83	4e-34	125
LLPS-Scc-1334	Schizosaccharomyces cryophilus	62.59	9e-46	154
LLPS-Nia-0735	Nicotiana attenuata	62.59	8e-46	156
LLPS-Chr-0249	Chlamydomonas reinhardtii	62.32	2e-43	151
LLPS-Asg-0618	Ashbya gossypii	62.16	8e-33	121
LLPS-Crn-0143	Cryptococcus neoformans	61.87	1e-41	144
LLPS-Usm-0442	Ustilago maydis	61.15	4e-39	139
LLPS-Map-0288	Magnaporthe poae	60.14	4e-45	152
LLPS-Fuo-0090	Fusarium oxysporum	59.71	1e-41	143
LLPS-Osl-0693	Ostreococcus lucimarinus	58.57	6e-42	144
LLPS-Miv-0176	Microbotryum violaceum	58.52	4e-40	140
LLPS-Scp-0244	Schizosaccharomyces pombe	57.97	1e-38	135
LLPS-Ere-0343	Erinaceus europaeus	55.65	8e-42	143
LLPS-Asn-1291	Aspergillus nidulans	51.02	1e-30	115
LLPS-Cym-0233	Cyanidioschyzon merolae	49.56	3e-34	123
LLPS-Kop-0155	Komagataella pastoris	45.65	5e-20	88.6
LLPS-Icp-3746	Ictalurus punctatus	38.37	2e-10	60.8
LLPS-Aso-0626	Aspergillus oryzae	35.06	6e-09	55.5

LLPS-Tut-1167NCBP2

Protein Sequence

Nucleotide Sequence

LLPS-Tut-1167
NCBP2