LLPS-Trv-0370
NCS2
Integrated Annotations
▼ OVERVIEW
| Status: | Unreviewed |
| Protein Name: | Cytoplasmic tRNA 2-thiolation protein 2 |
| Gene Name: | NCS2, CTU2, TRIVIDRAFT_52093 |
| Ensembl Gene: | TRIVIDRAFT_52093 |
| Ensembl Protein: | EHK21277 |
| Organism: | Trichoderma virens |
| Taxa ID: | 413071 |
| LLPS Type: | Others |
▼ Classification
Condensates:
| Condensate | Evidence | Orthologs |
|---|---|---|
| Stress granule | Predicted from orthologs | (View) |
▼ FUNCTION
| Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by forming a heterodimer with NCS6 that ligates sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. Prior mcm(5) tRNA modification by the elongator complex is required for 2-thiolation. May also be involved in protein urmylation. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | EHK21277 | EHK21277 |
| UniProt | G9MWE0, G9MWE0_HYPVG | |
| GeneBank | ABDF02000073 | EHK21277.1 |
| RefSeq | XM_014099995.1 | XP_013955470.1 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MAGSQVPSRP CMRCKAPDAA FELRNVATCQ FTVLQRETRT STKPEPRRYV AGLSFGPSST 60 61 VLTQILDKTA KYHASRKGSS PFEPLVVHID TDLSNRTDSQ DTPAQRLLAK YRERFPNVSF 120 121 ECIHLSKVLS VKTIDWTALP VPAGESDAER LQLFFSSLPS VTSRADSLRL FIRHLLLDVA 180 181 VERSHSALLF GHSTTALAAL TLSEVANGRG FAVPWQINDG LFTVCTYRPS ADPASAGEAS 240 241 QEDAKIQFPI YYPLREILKA EVMTYLGTTP SVQELVPSAD GAASSVVSHK DQSIEEVMAR 300 301 YFDGVEGPYS GIVTNVVRTA GKLDRLVTSH YCRLCGITLD QEGDSTWAGE LGDDLVQDAG 360 361 SADTGKLCYG CKRSMGG 377 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGGCGGGCT CACAAGTCCC CAGCCGGCCA TGCATGCGCT GCAAGGCACC AGATGCCGCA 60 61 TTTGAACTGC GCAATGTTGC AACCTGCCAA TTCACCGTTC TCCAGCGGGA AACCAGAACT 120 121 TCTACAAAGC CTGAGCCGCG GAGATACGTC GCTGGCCTTT CCTTTGGACC ATCCTCTACT 180 181 GTTCTCACCC AAATTCTGGA CAAGACGGCC AAATATCATG CATCAAGAAA GGGATCATCA 240 241 CCCTTTGAGC CCCTCGTGGT TCATATTGAT ACAGATCTTT CCAACCGCAC AGACTCCCAA 300 301 GATACCCCCG CACAGAGACT TTTAGCCAAG TACCGCGAGA GATTCCCCAA CGTCTCGTTT 360 361 GAGTGCATAC ACTTGAGCAA GGTTCTCAGT GTCAAGACGA TTGACTGGAC TGCACTACCG 420 421 GTACCTGCGG GTGAAAGCGA TGCAGAGCGG CTACAGCTGT TCTTTTCCTC GCTGCCGTCA 480 481 GTGACTTCGC GCGCTGATAG TCTGAGACTG TTCATTCGCC ATCTTCTCCT CGACGTGGCC 540 541 GTGGAAAGAT CTCATTCTGC CTTGCTGTTT GGCCACAGCA CCACTGCGCT GGCCGCCCTG 600 601 ACCTTGTCCG AGGTGGCAAA CGGCAGAGGC TTCGCTGTGC CTTGGCAGAT CAACGACGGA 660 661 CTCTTCACCG TCTGCACCTA TCGGCCGAGT GCAGATCCGG CTAGTGCCGG TGAGGCAAGC 720 721 CAAGAAGATG CCAAGATTCA GTTTCCCATC TACTATCCCC TGCGAGAGAT TCTAAAGGCC 780 781 GAAGTCATGA CCTATCTTGG CACGACGCCC TCTGTCCAGG AACTGGTCCC CTCTGCCGAC 840 841 GGAGCAGCGT CTAGCGTAGT TTCTCACAAG GACCAGAGCA TTGAAGAGGT CATGGCTCGC 900 901 TATTTCGACG GCGTGGAGGG CCCTTACTCG GGCATTGTGA CCAATGTCGT CCGCACGGCG 960 961 GGTAAGCTGG ACCGGCTGGT TACTTCGCAC TATTGTCGCT TATGCGGCAT CACCCTGGAC 1020 1021 CAAGAGGGCG ATTCAACATG GGCTGGCGAG CTGGGGGATG ACCTGGTACA AGACGCTGGG 1080 1081 AGCGCTGATA CGGGGAAGCT GTGTTACGGA TGCAAGCGTT CCATGGGTGG ATAG 1134 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Cytoplasm | |
Molecular Function | Nucleotidyltransferase activity | |
Molecular Function | TRNA binding | |
Biological Process | Protein urmylation | |
Biological Process | TRNA thio-modification | |
Biological Process | TRNA wobble uridine modification |
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Trr-1285 | Trichoderma reesei | 79.37 | 0.0 | 602 |
| LLPS-Fus-1476 | Fusarium solani | 55.93 | 1e-140 | 412 |
| LLPS-Beb-0158 | Beauveria bassiana | 51.37 | 1e-142 | 418 |
| LLPS-Ved-1499 | Verticillium dahliae | 41.53 | 4e-78 | 252 |
| LLPS-Phn-1462 | Phaeosphaeria nodorum | 32.66 | 6e-37 | 143 |
| LLPS-Lem-1028 | Leptosphaeria maculans | 32.24 | 1e-42 | 160 |
| LLPS-Blg-0422 | Blumeria graminis | 31.7 | 6e-34 | 134 |
| LLPS-Scj-1141 | Schizosaccharomyces japonicus | 31.09 | 5e-18 | 88.6 |
| LLPS-Pyt-1000 | Pyrenophora teres | 30.88 | 2e-36 | 141 |
| LLPS-Asg-1288 | Ashbya gossypii | 29.69 | 7e-12 | 70.5 |
| LLPS-Pytr-1288 | Pyrenophora triticirepentis | 29.43 | 2e-36 | 141 |
| LLPS-Tum-1515 | Tuber melanosporum | 29.36 | 3e-39 | 149 |
| LLPS-Asni-0195 | Aspergillus niger | 28.32 | 3e-32 | 129 |
| LLPS-Ast-0616 | Aspergillus terreus | 27.92 | 9e-24 | 104 |
| LLPS-Zyt-1362 | Zymoseptoria tritici | 27.88 | 2e-25 | 108 |
| LLPS-Dos-1484 | Dothistroma septosporum | 27.79 | 8e-30 | 122 |
| LLPS-Asc-1554 | Aspergillus clavatus | 27.75 | 5e-32 | 129 |
| LLPS-Nef-1586 | Neosartorya fischeri | 27.57 | 5e-26 | 111 |
| LLPS-Asn-1530 | Aspergillus nidulans | 27.11 | 2e-20 | 94.0 |
| LLPS-Kop-0030 | Komagataella pastoris | 26.9 | 6e-15 | 80.1 |
| LLPS-Dar-2867 | Danio rerio | 26.67 | 4e-08 | 58.9 |
| LLPS-Aso-0437 | Aspergillus oryzae | 26.57 | 2e-26 | 112 |
| LLPS-Asf-0774 | Aspergillus flavus | 26.57 | 2e-26 | 112 |
| LLPS-Scc-0397 | Schizosaccharomyces cryophilus | 26.0 | 1e-16 | 84.3 |
| LLPS-Yal-1371 | Yarrowia lipolytica | 24.8 | 7e-18 | 88.6 |
| LLPS-Scp-1628 | Schizosaccharomyces pombe | 24.32 | 9e-16 | 81.6 |
| LLPS-Sac-0470 | Saccharomyces cerevisiae | 23.44 | 7e-11 | 67.8 |