LLPS-Scp-1452
trm12
Integrated Annotations
▼ OVERVIEW
| Status: | Unreviewed |
| Protein Name: | tRNA wybutosine-synthesizing protein 2 |
| Gene Name: | trm12, tyw2, SPAC4G8.06c |
| Ensembl Gene: | SPAC4G8.06c |
| Ensembl Protein: | SPAC4G8.06c.1 |
| Organism: | Schizosaccharomyces pombe |
| Taxa ID: | 284812 |
| LLPS Type: | LLPS regulator |
▼ Classification
Condensates:
| Condensate | Evidence | Orthologs |
|---|---|---|
| Stress granule | Predicted from orthologs | (View) |
▼ FUNCTION
| S-adenosyl-L-methionine-dependent transferase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to the C-7 position of 4-demethylwyosine (imG-14) to produce wybutosine-86 (By similarity). |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | SPAC4G8.06c.1 | SPAC4G8.06c.1 |
| UniProt | Q09832, TYW2_SCHPO | |
| GeneBank | CU329670 | CAA91207.1 |
| RefSeq | NM_001018464.2 | NP_593066.1 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MISFLVQKSE AKYWKDVVQN KNKFLKNIGI ISGPKLVDTY SNQFEADFLK KCVLIPTSYN 60 61 KEDLPKDILD CPRIYLIEEE VKSVQTDEKL QIKVLRYVSQ ILHTTVSEEQ YPVPSRFVIY 120 121 PPLALLSLNS FGTKEWHLFF QSYANVDVKA GLFDIFAREW NVSHIAINEP IPVGDVMRRP 180 181 LSLKPLYGDF GVLIDGNPSE QDFQKAFWVS CRQNGIYQTW APLYTMFSRG NSIEKARVLN 240 241 FSYIKDEIIA DLYAGIGYFT FSYVKAGAST VFCWEINPWS VEALRRAALK NGWSIYVVRN 300 301 GENYEFKVGT HRIVVFLESN VYAAERFSKM NISARHINLG MLPSSEKSWS TATSILKRES 360 361 HSFIHVHENV KDEDIETYSS EVNSCFSKML AKNTVCVTNC VKSFSPRVSH IVYDIETV 418 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGATTTCTT TTCTAGTACA AAAGTCTGAA GCAAAGTACT GGAAGGATGT TGTTCAAAAT 60 61 AAAAATAAGT TTCTTAAAAA TATTGGGATC ATTTCGGGAC CTAAATTAGT TGACACCTAC 120 121 AGCAATCAAT TTGAAGCAGA TTTTCTCAAA AAGTGCGTTC TTATTCCAAC TTCTTATAAC 180 181 AAAGAAGACC TTCCTAAAGA TATATTAGAT TGCCCAAGAA TTTATTTAAT CGAAGAGGAG 240 241 GTAAAATCTG TTCAAACAGA TGAAAAATTG CAGATTAAAG TTCTTCGCTA TGTATCTCAA 300 301 ATCCTTCACA CAACTGTGAG TGAAGAACAA TATCCCGTTC CATCTAGGTT TGTCATTTAT 360 361 CCTCCGTTAG CTCTATTATC TTTAAACAGT TTTGGCACTA AAGAATGGCA TCTTTTTTTT 420 421 CAGTCTTATG CAAACGTCGA TGTCAAAGCT GGTTTATTTG ATATTTTTGC TAGAGAATGG 480 481 AACGTATCAC ATATTGCTAT TAATGAACCT ATTCCTGTGG GGGATGTAAT GAGAAGACCA 540 541 TTGTCATTGA AACCGCTATA CGGTGACTTT GGAGTGCTAA TTGATGGCAA CCCCTCTGAA 600 601 CAGGATTTTC AAAAAGCATT TTGGGTAAGT TGTCGTCAAA ACGGTATTTA TCAGACATGG 660 661 GCTCCTCTTT ATACGATGTT CTCTCGTGGT AATTCGATTG AAAAGGCCAG AGTACTTAAT 720 721 TTTAGTTACA TTAAAGACGA AATTATTGCA GATCTTTACG CTGGTATAGG GTACTTTACA 780 781 TTTTCTTACG TTAAAGCGGG AGCTTCAACA GTTTTTTGCT GGGAAATTAA TCCATGGAGT 840 841 GTTGAAGCGT TAAGGAGAGC TGCTTTGAAA AATGGCTGGT CTATTTATGT AGTTCGAAAT 900 901 GGAGAAAACT ATGAATTTAA AGTAGGAACT CATCGTATTG TAGTTTTTTT GGAAAGCAAC 960 961 GTTTATGCAG CTGAAAGGTT CTCCAAAATG AATATTTCTG CTCGACATAT AAATTTGGGA 1020 1021 ATGCTCCCCT CTAGTGAAAA ATCCTGGTCA ACCGCTACTT CAATTTTAAA GCGAGAATCA 1080 1081 CATTCCTTTA TACATGTTCA TGAGAACGTA AAAGATGAAG ATATCGAAAC ATACTCTTCT 1140 1141 GAAGTGAATT CATGTTTTTC AAAAATGTTG GCTAAAAATA CAGTTTGTGT TACAAATTGT 1200 1201 GTCAAATCAT TTTCACCACG AGTGTCACAT ATCGTTTACG ATATAGAGAC GGTTTAG 1257 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Cytoplasm | |
Cellular Component | Cytosol | |
Cellular Component | Nucleus | |
Molecular Function | S-adenosylmethionine-dependent methyltransferase activity | |
Molecular Function | TRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity | |
Molecular Function | TRNA methyltransferase activity | |
Biological Process | TRNA methylation | |
Biological Process | Wybutosine biosynthetic process |
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Scj-0079 | Schizosaccharomyces japonicus | 46.56 | 8e-132 | 393 |
| LLPS-Cog-1259 | Colletotrichum gloeosporioides | 42.57 | 4e-67 | 224 |
| LLPS-Fus-0753 | Fusarium solani | 41.0 | 3e-71 | 235 |
| LLPS-Trr-1517 | Trichoderma reesei | 40.7 | 5e-69 | 231 |
| LLPS-Fuv-1033 | Fusarium verticillioides | 40.24 | 3e-69 | 230 |
| LLPS-Blg-0598 | Blumeria graminis | 39.83 | 1e-72 | 240 |
| LLPS-Kop-0558 | Komagataella pastoris | 39.56 | 8e-71 | 236 |
| LLPS-Beb-0774 | Beauveria bassiana | 39.47 | 1e-64 | 218 |
| LLPS-Nec-1299 | Neurospora crassa | 39.38 | 6e-24 | 107 |
| LLPS-Mao-1552 | Magnaporthe oryzae | 39.32 | 2e-63 | 217 |
| LLPS-Trv-1534 | Trichoderma virens | 39.17 | 8e-71 | 235 |
| LLPS-Fuo-0462 | Fusarium oxysporum | 39.04 | 3e-67 | 225 |
| LLPS-Gag-0976 | Gaeumannomyces graminis | 38.98 | 4e-60 | 207 |
| LLPS-Cogr-1137 | Colletotrichum graminicola | 38.83 | 1e-68 | 229 |
| LLPS-Zyt-1116 | Zymoseptoria tritici | 38.72 | 2e-66 | 223 |
| LLPS-Ast-1176 | Aspergillus terreus | 38.67 | 4e-68 | 229 |
| LLPS-Dos-0766 | Dothistroma septosporum | 38.44 | 5e-64 | 216 |
| LLPS-Pes-0514 | Pelodiscus sinensis | 38.18 | 7e-22 | 98.2 |
| LLPS-Tum-1585 | Tuber melanosporum | 37.92 | 1e-56 | 199 |
| LLPS-Asc-0262 | Aspergillus clavatus | 37.85 | 1e-63 | 217 |
| LLPS-Nef-0999 | Neosartorya fischeri | 37.57 | 4e-61 | 211 |
| LLPS-Asfu-1267 | Aspergillus fumigatus | 37.36 | 2e-58 | 203 |
| LLPS-Pytr-1289 | Pyrenophora triticirepentis | 37.09 | 2e-68 | 232 |
| LLPS-Tag-1315 | Taeniopygia guttata | 36.97 | 6e-19 | 92.0 |
| LLPS-Phn-0969 | Phaeosphaeria nodorum | 36.87 | 1e-62 | 214 |
| LLPS-Yal-1001 | Yarrowia lipolytica | 36.8 | 5e-58 | 201 |
| LLPS-Coo-0636 | Colletotrichum orbiculare | 36.77 | 1e-61 | 211 |
| LLPS-Pyt-1120 | Pyrenophora teres | 36.69 | 1e-67 | 230 |
| LLPS-Ved-1457 | Verticillium dahliae | 36.43 | 3e-63 | 216 |
| LLPS-Aso-0828 | Aspergillus oryzae | 36.34 | 6e-69 | 231 |
| LLPS-Asf-1157 | Aspergillus flavus | 36.34 | 6e-69 | 231 |
| LLPS-Asni-1450 | Aspergillus niger | 36.04 | 1e-61 | 211 |
| LLPS-Asn-1528 | Aspergillus nidulans | 35.81 | 4e-64 | 218 |
| LLPS-Fia-1118 | Ficedula albicollis | 35.54 | 1e-19 | 92.4 |
| LLPS-Scs-0316 | Sclerotinia sclerotiorum | 35.0 | 2e-45 | 166 |
| LLPS-Meg-1120 | Meleagris gallopavo | 34.55 | 1e-13 | 76.3 |
| LLPS-Gaa-0838 | Gasterosteus aculeatus | 34.07 | 6e-24 | 105 |
| LLPS-Asg-0554 | Ashbya gossypii | 33.64 | 1e-54 | 193 |
| LLPS-Ten-2689 | Tetraodon nigroviridis | 32.78 | 2e-21 | 99.0 |
| LLPS-Orn-2148 | Oreochromis niloticus | 32.64 | 3e-22 | 101 |
| LLPS-Sac-0507 | Saccharomyces cerevisiae | 32.4 | 3e-51 | 185 |
| LLPS-Lem-1538 | Leptosphaeria maculans | 32.13 | 1e-55 | 201 |
| LLPS-Pof-2070 | Poecilia formosa | 31.67 | 1e-16 | 85.5 |
| LLPS-Tar-3961 | Takifugu rubripes | 31.54 | 4e-21 | 98.6 |
| LLPS-Orl-1489 | Oryzias latipes | 31.38 | 2e-21 | 99.0 |
| LLPS-Mel-0735 | Melampsora laricipopulina | 31.05 | 1e-31 | 129 |
| LLPS-Ora-3370 | Ornithorhynchus anatinus | 30.91 | 2e-09 | 63.2 |
| LLPS-Icp-4180 | Ictalurus punctatus | 30.87 | 8e-18 | 89.0 |
| LLPS-Myl-1502 | Myotis lucifugus | 30.56 | 7e-21 | 98.2 |
| LLPS-Scm-3230 | Scophthalmus maximus | 30.45 | 3e-20 | 95.9 |
| LLPS-Mup-1002 | Mustela putorius furo | 30.31 | 4e-20 | 95.9 |
| LLPS-Asm-3912 | Astyanax mexicanus | 30.25 | 2e-16 | 84.7 |
| LLPS-Xet-0080 | Xenopus tropicalis | 30.21 | 2e-19 | 93.6 |
| LLPS-Fud-0916 | Fukomys damarensis | 30.2 | 4e-23 | 104 |
| LLPS-Dar-2998 | Danio rerio | 30.13 | 1e-17 | 88.2 |
| LLPS-Pug-1469 | Puccinia graminis | 29.82 | 6e-32 | 130 |
| LLPS-Poa-3389 | Pongo abelii | 29.82 | 3e-18 | 90.5 |
| LLPS-Lac-1787 | Latimeria chalumnae | 29.79 | 1e-17 | 89.0 |
| LLPS-Hos-4174 | Homo sapiens | 29.72 | 2e-18 | 90.9 |
| LLPS-Loa-1752 | Loxodonta africana | 29.45 | 6e-21 | 98.2 |
| LLPS-Aim-4267 | Ailuropoda melanoleuca | 29.37 | 1e-19 | 94.4 |
| LLPS-Mam-3472 | Macaca mulatta | 29.37 | 5e-18 | 89.7 |
| LLPS-Mum-3254 | Mus musculus | 29.33 | 9e-19 | 92.0 |
| LLPS-Gaga-2603 | Gallus gallus | 29.17 | 2e-16 | 85.5 |
| LLPS-Ran-2106 | Rattus norvegicus | 29.12 | 8e-21 | 97.8 |
| LLPS-Caf-3823 | Canis familiaris | 29.12 | 9e-21 | 97.4 |
| LLPS-Chs-3691 | Chlorocebus sabaeus | 29.02 | 2e-17 | 88.2 |
| LLPS-Orc-3156 | Oryctolagus cuniculus | 29.01 | 5e-22 | 101 |
| LLPS-Cap-2865 | Cavia porcellus | 28.87 | 1e-18 | 91.7 |
| LLPS-Otg-2969 | Otolemur garnettii | 28.57 | 3e-20 | 95.9 |
| LLPS-Osl-0830 | Ostreococcus lucimarinus | 28.53 | 2e-18 | 89.0 |
| LLPS-Scf-1078 | Scleropages formosus | 28.21 | 5e-24 | 106 |
| LLPS-Xim-0194 | Xiphophorus maculatus | 27.94 | 8e-19 | 91.7 |
| LLPS-Bot-2270 | Bos taurus | 27.89 | 2e-19 | 93.6 |
| LLPS-Cis-0403 | Ciona savignyi | 27.85 | 1e-12 | 72.0 |
| LLPS-Caj-3914 | Callithrix jacchus | 27.25 | 2e-19 | 94.0 |
| LLPS-Sah-3768 | Sarcophilus harrisii | 27.01 | 4e-19 | 92.8 |
| LLPS-Ict-1736 | Ictidomys tridecemlineatus | 26.57 | 1e-19 | 94.7 |
| LLPS-Mod-0131 | Monodelphis domestica | 26.5 | 5e-18 | 89.7 |
| LLPS-Sem-2108 | Selaginella moellendorffii | 26.43 | 1e-18 | 90.5 |
| LLPS-Ere-0997 | Erinaceus europaeus | 24.03 | 2e-19 | 94.0 |