LLPS-Sac-1276
PDC1

▼ OVERVIEW

Status: Unreviewed
Protein Name: Pyruvate decarboxylase isozyme 1
Gene Name: PDC1, YLR044C, L2104
Ensembl Gene: YLR044C
Ensembl Protein: YLR044C_mRNA
Organism: Saccharomyces cerevisiae
Taxa ID: 559292
LLPS Type: Scaffold
PDB: 1PYD (A) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
Droplet, P-bodyPredicted from orthologs(View)

▼ FUNCTION

Major of three pyruvate decarboxylases (PDC1, PDC5, PDC6) implicated in the nonoxidative conversion of pyruvate to acetaldehyde and carbon dioxide during alcoholic fermentation. Most of the produced acetaldehyde is subsequently reduced to ethanol, but some is required for cytosolic acetyl-CoA production for biosynthetic pathways. The enzyme is also one of five 2-oxo acid decarboxylases (PDC1, PDC5, PDC6, ARO10, and THI3) able to decarboxylate more complex 2-oxo acids (alpha-ketoacids) than pyruvate, which seem mainly involved in amino acid catabolism. Here the enzyme catalyzes the decarboxylation of amino acids, which, in a first step, have been transaminated to the corresponding 2-oxo acids. In a third step, the resulting aldehydes are reduced to alcohols, collectively referred to as fusel oils or alcohols. Its preferred substrates are the transaminated amino acids derived from threonine (2-oxobutanoate), norvaline (2-oxopentanoate), valine (3-methyl-2-oxobutanoate, also alpha-keto-isovalerate), isoleucine ((3S)-3-methyl-2-oxopentanoate, also alpha-keto-beta-methylvalerate), phenylalanine (phenylpyruvate), and tryptophan (3-(indol-3-yl)pyruvate), whereas transaminated leucine is no substrate. In a side-reaction the carbanionic intermediate (or active aldehyde) generated by decarboxylation or by activation of an aldehyde can react with an aldehyde via condensation (or carboligation) yielding a 2-hydroxy ketone, collectively called acyloins.

▼ SEQUENCE

Protein Sequence (FASTA)
1     MSEITLGKYL  FERLKQVNVN  TVFGLPGDFN  LSLLDKIYEV  EGMRWAGNAN  ELNAAYAADG  60
61    YARIKGMSCI  ITTFGVGELS  ALNGIAGSYA  EHVGVLHVVG  VPSISAQAKQ  LLLHHTLGNG  120
121   DFTVFHRMSA  NISETTAMIT  DIATAPAEID  RCIRTTYVTQ  RPVYLGLPAN  LVDLNVPAKL  180
181   LQTPIDMSLK  PNDAESEKEV  IDTILALVKD  AKNPVILADA  CCSRHDVKAE  TKKLIDLTQF  240
241   PAFVTPMGKG  SIDEQHPRYG  GVYVGTLSKP  EVKEAVESAD  LILSVGALLS  DFNTGSFSYS  300
301   YKTKNIVEFH  SDHMKIRNAT  FPGVQMKFVL  QKLLTTIADA  AKGYKPVAVP  ARTPANAAVP  360
361   ASTPLKQEWM  WNQLGNFLQE  GDVVIAETGT  SAFGINQTTF  PNNTYGISQV  LWGSIGFTTG  420
421   ATLGAAFAAE  EIDPKKRVIL  FIGDGSLQLT  VQEISTMIRW  GLKPYLFVLN  NDGYTIEKLI  480
481   HGPKAQYNEI  QGWDHLSLLP  TFGAKDYETH  RVATTGEWDK  LTQDKSFNDN  SKIRMIEIML  540
541   PVFDAPQNLV  EQAKLTAATN  AKQ  563
Nucleotide CDS Sequence (FASTA)
1     ATGTCTGAAA  TTACTTTGGG  TAAATATTTG  TTCGAAAGAT  TAAAGCAAGT  CAACGTTAAC  60
61    ACCGTTTTCG  GTTTGCCAGG  TGACTTCAAC  TTGTCCTTGT  TGGACAAGAT  CTACGAAGTT  120
121   GAAGGTATGA  GATGGGCTGG  TAACGCCAAC  GAATTGAACG  CTGCTTACGC  CGCTGATGGT  180
181   TACGCTCGTA  TCAAGGGTAT  GTCTTGTATC  ATCACCACCT  TCGGTGTCGG  TGAATTGTCT  240
241   GCTTTGAACG  GTATTGCCGG  TTCTTACGCT  GAACACGTCG  GTGTTTTGCA  CGTTGTTGGT  300
301   GTCCCATCCA  TCTCTGCTCA  AGCTAAGCAA  TTGTTGTTGC  ACCACACCTT  GGGTAACGGT  360
361   GACTTCACTG  TTTTCCACAG  AATGTCTGCC  AACATTTCTG  AAACCACTGC  TATGATCACT  420
421   GACATTGCTA  CCGCCCCAGC  TGAAATTGAC  AGATGTATCA  GAACCACTTA  CGTCACCCAA  480
481   AGACCAGTCT  ACTTAGGTTT  GCCAGCTAAC  TTGGTCGACT  TGAACGTCCC  AGCTAAGTTG  540
541   TTGCAAACTC  CAATTGACAT  GTCTTTGAAG  CCAAACGATG  CTGAATCCGA  AAAGGAAGTC  600
601   ATTGACACCA  TCTTGGCTTT  GGTCAAGGAT  GCTAAGAACC  CAGTTATCTT  GGCTGATGCT  660
661   TGTTGTTCCA  GACACGACGT  CAAGGCTGAA  ACTAAGAAGT  TGATTGACTT  GACTCAATTC  720
721   CCAGCTTTCG  TCACCCCAAT  GGGTAAGGGT  TCCATTGACG  AACAACACCC  AAGATACGGT  780
781   GGTGTTTACG  TCGGTACCTT  GTCCAAGCCA  GAAGTTAAGG  AAGCCGTTGA  ATCTGCTGAC  840
841   TTGATTTTGT  CTGTCGGTGC  TTTGTTGTCT  GATTTCAACA  CCGGTTCTTT  CTCTTACTCT  900
901   TACAAGACCA  AGAACATTGT  CGAATTCCAC  TCCGACCACA  TGAAGATCAG  AAACGCCACT  960
961   TTCCCAGGTG  TCCAAATGAA  ATTCGTTTTG  CAAAAGTTGT  TGACCACTAT  TGCTGACGCC  1020
1021  GCTAAGGGTT  ACAAGCCAGT  TGCTGTCCCA  GCTAGAACTC  CAGCTAACGC  TGCTGTCCCA  1080
1081  GCTTCTACCC  CATTGAAGCA  AGAATGGATG  TGGAACCAAT  TGGGTAACTT  CTTGCAAGAA  1140
1141  GGTGATGTTG  TCATTGCTGA  AACCGGTACC  TCCGCTTTCG  GTATCAACCA  AACCACTTTC  1200
1201  CCAAACAACA  CCTACGGTAT  CTCTCAAGTC  TTATGGGGTT  CCATTGGTTT  CACCACTGGT  1260
1261  GCTACCTTGG  GTGCTGCTTT  CGCTGCTGAA  GAAATTGATC  CAAAGAAGAG  AGTTATCTTA  1320
1321  TTCATTGGTG  ACGGTTCTTT  GCAATTGACT  GTTCAAGAAA  TCTCCACCAT  GATCAGATGG  1380
1381  GGCTTGAAGC  CATACTTGTT  CGTCTTGAAC  AACGATGGTT  ACACCATTGA  AAAGTTGATT  1440
1441  CACGGTCCAA  AGGCTCAATA  CAACGAAATT  CAAGGTTGGG  ACCACCTATC  CTTGTTGCCA  1500
1501  ACTTTCGGTG  CTAAGGACTA  TGAAACCCAC  AGAGTCGCTA  CCACCGGTGA  ATGGGACAAG  1560
1561  TTGACCCAAG  ACAAGTCTTT  CAACGACAAC  TCTAAGATCA  GAATGATTGA  AATCATGTTG  1620
1621  CCAGTCTTCG  ATGCTCCACA  AAACTTGGTT  GAACAAGCTA  AGTTGACTGC  TGCTACCAAC  1680
1681  GCTAAGCAAT  AA  1692

▼ KEYWORD

ID
Family
3D-structure
Acetylation
Allosteric enzyme
Branched-chain amino acid catabolism
Complete proteome
Cytoplasm
Decarboxylase
Direct protein sequencing
Isopeptide bond
Lyase
Magnesium
Metal-binding
Nucleus
Phenylalanine catabolism
Phosphoprotein
Reference proteome
Thiamine pyrophosphate
Tryptophan catabolism
Ubl conjugation

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cytoplasm
Cellular Component
Cytosol
Cellular Component
Nucleus
Molecular Function
Branched-chain-2-oxoacid decarboxylase activity
Molecular Function
Carboxy-lyase activity
Molecular Function
Indolepyruvate decarboxylase activity
Molecular Function
Magnesium ion binding
Molecular Function
Phenylpyruvate decarboxylase activity
Molecular Function
Pyruvate decarboxylase activity
Molecular Function
Thiamine pyrophosphate binding
Biological Process
Aromatic amino acid family catabolic process to alcohol via Ehrlich pathway
Biological Process
Branched-chain amino acid catabolic process
Biological Process
Ethanol metabolic process
Biological Process
Glycolytic fermentation to ethanol
Biological Process
L-phenylalanine catabolic process
Biological Process
Pyruvate metabolic process
Biological Process
Tryptophan catabolic process

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Asg-1470Ashbya gossypii83.480.0 952
LLPS-Kop-0705Komagataella pastoris64.070.0 731
LLPS-Yal-1288Yarrowia lipolytica57.520.0 618
LLPS-Tum-0917Tuber melanosporum51.960.0 556
LLPS-Scc-1376Schizosaccharomyces cryophilus51.590.0 568
LLPS-Scj-0857Schizosaccharomyces japonicus50.350.0 568
LLPS-Scp-0158Schizosaccharomyces pombe49.740.0 535
LLPS-Scs-1117Sclerotinia sclerotiorum49.650.0 547
LLPS-Lem-1265Leptosphaeria maculans49.363e-174 513
LLPS-Trv-1665Trichoderma virens48.580.0 533
LLPS-Asf-0927Aspergillus flavus48.43e-177 520
LLPS-Aso-1063Aspergillus oryzae48.43e-177 520
LLPS-Ast-1496Aspergillus terreus48.235e-174 512
LLPS-Trr-1032Trichoderma reesei47.952e-179 526
LLPS-Pyt-1435Pyrenophora teres47.874e-170 502
LLPS-Cogr-1345Colletotrichum graminicola47.873e-179 526
LLPS-Beb-1205Beauveria bassiana47.864e-180 528
LLPS-Pytr-1526Pyrenophora triticirepentis47.641e-167 496
LLPS-Fus-0260Fusarium solani47.518e-175 514
LLPS-Fuo-1284Fusarium oxysporum47.514e-173 509
LLPS-Asn-1498Aspergillus nidulans47.316e-172 506
LLPS-Nef-1513Neosartorya fischeri47.236e-174 512
LLPS-Asc-1662Aspergillus clavatus47.227e-171 504
LLPS-Asfu-1478Aspergillus fumigatus47.053e-173 510
LLPS-Ved-1362Verticillium dahliae47.02e-179 526
LLPS-Fuv-0501Fusarium verticillioides46.982e-172 508
LLPS-Phn-1049Phaeosphaeria nodorum46.841e-168 498
LLPS-Nec-0300Neurospora crassa46.521e-180 529
LLPS-Cog-1022Colletotrichum gloeosporioides45.733e-162 492
LLPS-Dos-1099Dothistroma septosporum42.184e-145 437
LLPS-Abg-1325Absidia glauca41.132e-131 402
LLPS-Via-1502Vigna angularis40.484e-33 134
LLPS-Dac-2093Daucus carota39.561e-1892.4
LLPS-Asni-1098Aspergillus niger37.853e-121 376
LLPS-Ori-1974Oryza indica36.123e-98 316
LLPS-Sot-0345Solanum tuberosum34.388e-75 255
LLPS-Cus-1224Cucumis sativus33.912e-80 270
LLPS-Mao-1295Magnaporthe oryzae33.672e-83 278
LLPS-Ors-2225Oryza sativa33.13e-78 265
LLPS-Hov-2154Hordeum vulgare33.042e-77 265
LLPS-Orni-2296Oryza nivara32.861e-77 263
LLPS-Orb-2412Oryza barthii32.865e-78 264
LLPS-Orgl-1334Oryza glumaepatula32.867e-78 264
LLPS-Sob-0196Sorghum bicolor32.861e-77 263
LLPS-Orm-1840Oryza meridionalis32.864e-78 264
LLPS-Orr-1020Oryza rufipogon32.865e-78 264
LLPS-Tra-0541Triticum aestivum32.692e-78 265
LLPS-Sei-0038Setaria italica32.699e-77 261
LLPS-Orp-0661Oryza punctata32.695e-78 264
LLPS-Zem-2629Zea mays32.694e-78 264
LLPS-Hea-2383Helianthus annuus32.631e-76 259
LLPS-Mae-2164Manihot esculenta32.459e-78 263
LLPS-Prp-1008Prunus persica32.42e-76 259
LLPS-Brd-2488Brachypodium distachyon32.341e-73 255
LLPS-Mua-2603Musa acuminata30.533e-64 226
LLPS-Nia-2210Nicotiana attenuata28.191e-0964.7
LLPS-Sol-0725Solanum lycopersicum27.483e-0963.9
LLPS-Met-2586Medicago truncatula27.113e-0860.8
LLPS-Arl-2569Arabidopsis lyrata26.837e-1168.9
LLPS-Gas-0520Galdieria sulphuraria25.952e-0964.7
LLPS-Amt-0176Amborella trichopoda25.431e-0861.6
LLPS-Pot-2266Populus trichocarpa25.335e-0653.5
LLPS-Art-0854Arabidopsis thaliana25.285e-1272.8
LLPS-Viv-1697Vitis vinifera25.12e-0861.2
LLPS-Php-0305Physcomitrella patens25.03e-0757.4
LLPS-Leo-2029Lepisosteus oculatus25.02e-1581.3
LLPS-Gor-2737Gossypium raimondii24.933e-0963.9
LLPS-Brn-1007Brassica napus24.856e-1272.4
LLPS-Coo-0889Colletotrichum orbiculare24.841e-0862.0
LLPS-Thc-1495Theobroma cacao24.77e-1065.9
LLPS-Map-0593Magnaporthe poae24.581e-0861.6
LLPS-Brr-1159Brassica rapa24.51e-0965.5
LLPS-Glm-0904Glycine max24.312e-0655.1
LLPS-Vir-1551Vigna radiata24.213e-0757.8
LLPS-Pof-2691Poecilia formosa24.025e-1685.5
LLPS-Spr-0712Sporisorium reilianum24.08e-0756.2
LLPS-Osl-0617Ostreococcus lucimarinus23.993e-0963.9
LLPS-Blg-0441Blumeria graminis23.873e-0860.8
LLPS-Crn-0225Cryptococcus neoformans23.864e-1170.1
LLPS-Gag-0030Gaeumannomyces graminis23.862e-0758.2
LLPS-Chr-0999Chlamydomonas reinhardtii23.835e-0963.2
LLPS-Phv-1420Phaseolus vulgaris23.791e-0758.5
LLPS-Mel-1230Melampsora laricipopulina23.64e-1169.7
LLPS-Pug-0711Puccinia graminis23.63e-1170.5
LLPS-Coc-2263Corchorus capsularis23.585e-0860.1
LLPS-Bro-1680Brassica oleracea23.442e-1067.8
LLPS-Zyt-0994Zymoseptoria tritici23.25e-0860.1
LLPS-Miv-0294Microbotryum violaceum23.082e-0964.7
LLPS-Put-0112Puccinia triticina22.842e-1170.9
LLPS-Chc-0693Chondrus crispus22.449e-1168.6
LLPS-Sem-0167Selaginella moellendorffii22.369e-0858.9
LLPS-Otg-3475Otolemur garnettii22.153e-1376.3
LLPS-Lac-3565Latimeria chalumnae20.825e-1169.3