LLPS-Sac-0927
PRP40

▼ OVERVIEW

Status: Unreviewed
Protein Name: Pre-mRNA-processing protein PRP40
Gene Name: PRP40, YKL012W, YKL165
Ensembl Gene: YKL012W
Ensembl Protein: YKL012W_mRNA
Organism: Saccharomyces cerevisiae
Taxa ID: 559292
LLPS Type: Others
PDB: 2B7E (A) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
NucleolusPredicted from orthologs(View)

▼ FUNCTION

Required for pre-spliceosome formation, which is the first step of pre-mRNA splicing. This protein is associated with snRNP U1. Two commitment complexes, CC1 and CC2, have been defined in yeast. CC1 is a basal complex dependent only on the 5' splice site. CC2 is a complex of lower mobility and is dependent on a branchpoint as well as a 5' splice site region. This protein is involved in CC2 formation where it binds to the branchpoint binding protein MSL5, bridging the U1 snRNP-associated 5' splice site and the MSL5-associated branch point 3' intron splice site.

▼ CROSS REFERENCE

▼ SEQUENCE

Protein Sequence (FASTA)
1     MSIWKEAKDA  SGRIYYYNTL  TKKSTWEKPK  ELISQEELLL  RENGWKAAKT  ADGKVYYYNP  60
61    TTRETSWTIP  AFEKKVEPIA  EQKHDTVSHA  QVNGNRIALT  AGEKQEPGRT  INEEESQYAN  120
121   NSKLLNVRRR  TKEEAEKEFI  TMLKENQVDS  TWSFSRIISE  LGTRDPRYWM  VDDDPLWKKE  180
181   MFEKYLSNRS  ADQLLKEHNE  TSKFKEAFQK  MLQNNSHIKY  YTRWPTAKRL  IADEPIYKHS  240
241   VVNEKTKRQT  FQDYIDTLID  TQKESKKKLK  TQALKELREY  LNGIITTSSS  ETFITWQQLL  300
301   NHYVFDKSKR  YMANRHFKVL  THEDVLNEYL  KIVNTIENDL  QNKLNELRLR  NYTRDRIARD  360
361   NFKSLLREVP  IKIKANTRWS  DIYPHIKSDP  RFLHMLGRNG  SSCLDLFLDF  VDEQRMYIFA  420
421   QRSIAQQTLI  DQNFEWNDAD  SDEITKQNIE  KVLENDRKFD  KVDKEDISLI  VDGLIKQRNE  480
481   KIQQKLQNER  RILEQKKHYF  WLLLQRTYTK  TGKPKPSTWD  LASKELGESL  EYKALGDEDN  540
541   IRRQIFEDFK  PESSAPTAES  ATANLTLTAS  KKRHLTPAVE  LDY  583
Nucleotide CDS Sequence (FASTA)
1     ATGTCTATTT  GGAAGGAAGC  TAAAGATGCA  AGTGGAAGGA  TATATTATTA  CAATACTTTG  60
61    ACAAAGAAAT  CTACGTGGGA  GAAGCCCAAG  GAACTAATTT  CTCAGGAGGA  GCTACTTCTT  120
121   CGAGAAAATG  GCTGGAAGGC  GGCCAAGACG  GCAGACGGCA  AAGTATACTA  TTATAATCCA  180
181   ACAACAAGAG  AAACCAGCTG  GACTATTCCG  GCCTTCGAGA  AGAAAGTAGA  ACCCATCGCA  240
241   GAACAAAAAC  ATGATACAGT  ATCCCATGCA  CAGGTTAATG  GAAATAGAAT  AGCCCTTACG  300
301   GCTGGAGAAA  AACAAGAGCC  GGGACGAACT  ATAAACGAGG  AGGAAAGCCA  ATATGCTAAT  360
361   AACTCTAAAC  TGCTTAATGT  CAGGAGAAGG  ACTAAAGAAG  AAGCAGAGAA  GGAATTTATT  420
421   ACCATGCTGA  AGGAAAATCA  AGTAGACTCT  ACTTGGTCAT  TCAGTAGAAT  TATTTCAGAA  480
481   CTGGGGACCA  GAGATCCAAG  GTATTGGATG  GTCGATGATG  ACCCCTTATG  GAAGAAAGAA  540
541   ATGTTTGAGA  AATATCTTTC  CAATAGATCA  GCCGATCAAC  TTCTTAAGGA  ACACAATGAA  600
601   ACAAGCAAAT  TCAAAGAAGC  CTTTCAGAAA  ATGTTGCAAA  ACAATTCTCA  TATAAAATAT  660
661   TACACCCGTT  GGCCTACCGC  AAAGAGACTA  ATTGCCGACG  AACCAATATA  CAAACACTCC  720
721   GTGGTCAATG  AAAAGACAAA  GAGACAGACC  TTTCAAGATT  ATATAGATAC  CCTCATCGAC  780
781   ACTCAGAAAG  AATCAAAAAA  AAAATTGAAA  ACACAGGCCC  TAAAAGAACT  AAGAGAGTAT  840
841   TTAAACGGTA  TTATAACAAC  ATCATCCTCT  GAAACTTTCA  TAACCTGGCA  GCAGCTTTTA  900
901   AATCACTATG  TTTTTGATAA  GAGTAAGAGA  TATATGGCGA  ACCGGCACTT  CAAAGTCTTA  960
961   ACCCACGAAG  ATGTTTTAAA  CGAGTATCTG  AAAATAGTAA  ATACGATTGA  AAACGATCTT  1020
1021  CAAAACAAAC  TAAATGAGCT  CCGACTGCGC  AATTATACCA  GAGACCGTAT  TGCTAGAGAT  1080
1081  AACTTTAAAA  GCTTATTAAG  AGAAGTGCCA  ATCAAAATCA  AAGCAAATAC  TAGATGGTCA  1140
1141  GATATTTATC  CTCATATAAA  GTCTGATCCG  CGCTTTTTAC  ATATGCTTGG  AAGGAATGGC  1200
1201  TCGTCCTGCC  TTGATTTATT  TTTAGATTTT  GTTGATGAAC  AAAGGATGTA  CATCTTTGCA  1260
1261  CAAAGATCAA  TAGCCCAACA  GACGTTGATA  GATCAAAATT  TTGAATGGAA  TGATGCCGAT  1320
1321  AGCGACGAGA  TCACCAAGCA  AAACATAGAA  AAAGTTCTGG  AAAATGACCG  GAAATTTGAC  1380
1381  AAGGTGGATA  AAGAAGACAT  CAGTTTGATT  GTTGATGGTT  TGATAAAGCA  AAGAAACGAA  1440
1441  AAGATACAAC  AGAAACTCCA  AAATGAGCGT  AGGATATTGG  AGCAAAAGAA  GCACTATTTT  1500
1501  TGGTTACTTT  TGCAAAGGAC  ATATACAAAA  ACCGGTAAGC  CCAAGCCTAG  TACGTGGGAT  1560
1561  TTAGCTTCCA  AAGAGCTTGG  CGAATCTCTT  GAATACAAGG  CACTAGGCGA  TGAAGATAAC  1620
1621  ATAAGAAGAC  AAATTTTCGA  GGATTTTAAG  CCTGAAAGCT  CTGCACCGAC  TGCCGAAAGC  1680
1681  GCTACTGCAA  ACTTAACGTT  GACCGCGTCA  AAAAAGAGGC  ATTTAACTCC  GGCTGTGGAA  1740
1741  TTGGACTATT  GA  1752

▼ KEYWORD

ID
Family
3D-structure
Complete proteome
Direct protein sequencing
mRNA processing
mRNA splicing
Nucleus
Phosphoprotein
Reference proteome
Repeat
Ribonucleoprotein

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Nucleus
Cellular Component
U1 snRNP
Cellular Component
U2-type prespliceosome
Molecular Function
RNA binding
Biological Process
MRNA cis splicing, via spliceosome
Biological Process
MRNA splicing, via spliceosome

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Xet-3656Xenopus tropicalis55.071e-1481.6
LLPS-Lac-3771Latimeria chalumnae53.629e-1478.6
LLPS-Ora-3518Ornithorhynchus anatinus52.173e-1377.0
LLPS-Chs-0264Chlorocebus sabaeus52.174e-1376.6
LLPS-Maf-3907Macaca fascicularis52.174e-1376.6
LLPS-Aon-4169Aotus nancymaae52.175e-1376.6
LLPS-Cea-1688Cercocebus atys52.174e-1376.6
LLPS-Sah-1930Sarcophilus harrisii52.174e-1376.6
LLPS-Sus-2042Sus scrofa52.175e-1376.6
LLPS-Gog-4469Gorilla gorilla52.174e-1376.6
LLPS-Caj-1070Callithrix jacchus52.174e-1376.6
LLPS-Fud-0755Fukomys damarensis52.173e-1377.0
LLPS-Asm-3780Astyanax mexicanus52.177e-1475.1
LLPS-Bot-4169Bos taurus52.174e-1377.0
LLPS-Dio-0073Dipodomys ordii52.174e-1377.0
LLPS-Aim-4312Ailuropoda melanoleuca52.174e-1376.6
LLPS-Mup-0613Mustela putorius furo52.174e-1377.0
LLPS-Ova-3552Ovis aries52.173e-1377.0
LLPS-Myl-3936Myotis lucifugus52.173e-1377.0
LLPS-Mal-2101Mandrillus leucophaeus52.174e-1376.6
LLPS-Paa-4031Papio anubis52.174e-1376.6
LLPS-Fec-2244Felis catus52.174e-1376.6
LLPS-Mod-0890Monodelphis domestica52.175e-1376.6
LLPS-Pat-4017Pan troglodytes52.174e-1376.6
LLPS-Pap-0846Pan paniscus52.174e-1376.6
LLPS-Hos-3065Homo sapiens52.174e-1376.6
LLPS-Anp-2886Anas platyrhynchos52.175e-1376.6
LLPS-Man-4047Macaca nemestrina52.174e-1376.6
LLPS-Pes-2498Pelodiscus sinensis52.175e-1376.6
LLPS-Otg-3242Otolemur garnettii52.173e-1377.4
LLPS-Rhb-0628Rhinopithecus bieti52.174e-1376.6
LLPS-Eqc-4604Equus caballus52.174e-1376.6
LLPS-Poa-1471Pongo abelii52.175e-1376.6
LLPS-Caf-4077Canis familiaris52.174e-1376.6
LLPS-Mam-1065Macaca mulatta52.174e-1377.0
LLPS-Gaga-2381Gallus gallus52.175e-1376.3
LLPS-Cas-3960Carlito syrichta52.174e-1377.0
LLPS-Ict-1251Ictidomys tridecemlineatus52.173e-1377.0
LLPS-Mea-3712Mesocricetus auratus51.471e-1275.5
LLPS-Mum-1666Mus musculus51.471e-1275.1
LLPS-Ran-3584Rattus norvegicus51.471e-1275.1
LLPS-Cap-0667Cavia porcellus51.471e-1275.5
LLPS-Art-0900Arabidopsis thaliana50.722e-1170.9
LLPS-Brr-2695Brassica rapa50.721e-1172.0
LLPS-Ten-1362Tetraodon nigroviridis50.729e-1375.5
LLPS-Bro-2586Brassica oleracea50.721e-1172.0
LLPS-Sol-1559Solanum lycopersicum49.283e-1170.9
LLPS-Tut-1690Tursiops truncatus49.288e-1272.4
LLPS-Arl-1118Arabidopsis lyrata49.288e-1169.3
LLPS-Urm-0302Ursus maritimus49.286e-1272.8
LLPS-Coc-1672Corchorus capsularis49.283e-1170.9
LLPS-Prp-1105Prunus persica49.281e-1172.4
LLPS-Drm-1669Drosophila melanogaster49.255e-1273.2
LLPS-Cus-0538Cucumis sativus47.835e-1170.1
LLPS-Via-1118Vigna angularis47.149e-1272.4
LLPS-Dac-1863Daucus carota46.388e-1169.3
LLPS-Thc-1748Theobroma cacao46.382e-1068.6
LLPS-Met-0083Medicago truncatula46.384e-1067.0
LLPS-Amt-2308Amborella trichopoda46.382e-1068.2
LLPS-Mae-0623Manihot esculenta46.383e-1067.4
LLPS-Glm-2141Glycine max46.273e-1067.4
LLPS-Cii-2357Ciona intestinalis46.271e-1171.6
LLPS-Phv-1110Phaseolus vulgaris45.712e-1170.9
LLPS-Hea-1917Helianthus annuus44.934e-0963.9
LLPS-Mua-0699Musa acuminata44.931e-1068.9
LLPS-Tra-2137Triticum aestivum44.781e-1068.6
LLPS-Tru-0857Triticum urartu44.781e-1068.6
LLPS-Asg-1304Ashbya gossypii43.652e-151 454
LLPS-Zem-2184Zea mays43.481e-0965.9
LLPS-Brd-1151Brachypodium distachyon43.281e-0965.9
LLPS-Orl-3308Oryzias latipes42.593e-1377.4
LLPS-Sem-1461Selaginella moellendorffii42.57e-0859.7
LLPS-Gag-1589Gaeumannomyces graminis42.031e-1068.6
LLPS-Map-1204Magnaporthe poae42.038e-1066.2
LLPS-Gor-2613Gossypium raimondii41.432e-0964.7
LLPS-Miv-0439Microbotryum violaceum41.433e-0860.8
LLPS-Nia-0915Nicotiana attenuata39.774e-0653.5
LLPS-Put-0180Puccinia triticina39.474e-0757.4
LLPS-Viv-0440Vitis vinifera39.222e-1067.8
LLPS-Orp-0904Oryza punctata39.183e-1170.5
LLPS-Chc-0037Chondrus crispus39.085e-1169.7
LLPS-Gaa-2508Gasterosteus aculeatus38.828e-0858.9
LLPS-Icp-0183Ictalurus punctatus38.464e-0653.5
LLPS-Nec-1513Neurospora crassa38.367e-1066.2
LLPS-Mao-0252Magnaporthe oryzae37.684e-1067.0
LLPS-Orn-2558Oreochromis niloticus37.663e-0653.9
LLPS-Vir-0758Vigna radiata37.187e-0756.2
LLPS-Beb-0734Beauveria bassiana37.141e-0862.4
LLPS-Usm-0889Ustilago maydis36.994e-0860.5
LLPS-Gas-1018Galdieria sulphuraria36.842e-0861.2
LLPS-Pof-0624Poecilia formosa36.716e-0756.2
LLPS-Lep-0499Leersia perrieri36.542e-1171.6
LLPS-Brn-0922Brassica napus36.522e-1068.6
LLPS-Sei-2313Setaria italica35.347e-1169.7
LLPS-Ori-0874Oryza indica35.292e-1068.6
LLPS-Orb-2211Oryza barthii35.291e-1068.6
LLPS-Orni-1435Oryza nivara35.291e-1068.6
LLPS-Orgl-1761Oryza glumaepatula35.291e-1068.9
LLPS-Orr-1134Oryza rufipogon35.291e-1068.6
LLPS-Sob-0449Sorghum bicolor34.755e-1067.0
LLPS-Coo-0074Colletotrichum orbiculare34.674e-0860.5
LLPS-Chr-0836Chlamydomonas reinhardtii34.578e-1169.3
LLPS-Pot-1426Populus trichocarpa34.267e-1066.2
LLPS-Xim-2670Xiphophorus maculatus33.041e-0758.5
LLPS-Mel-1456Melampsora laricipopulina32.616e-0653.5
LLPS-Orbr-1389Oryza brachyantha31.782e-1067.8
LLPS-Pug-0614Puccinia graminis31.537e-0756.6
LLPS-Scp-0681Schizosaccharomyces pombe30.492e-48 184
LLPS-Trv-1536Trichoderma virens29.489e-51 191
LLPS-Blg-1168Blumeria graminis29.083e-51 192
LLPS-Trr-0197Trichoderma reesei28.264e-48 183
LLPS-Tum-0573Tuber melanosporum28.256e-56 206
LLPS-Scc-0594Schizosaccharomyces cryophilus28.116e-41 161
LLPS-Asn-1112Aspergillus nidulans28.042e-47 181
LLPS-Phn-1350Phaeosphaeria nodorum28.038e-42 164
LLPS-Aso-0327Aspergillus oryzae27.898e-50 189
LLPS-Asf-0040Aspergillus flavus27.898e-50 189
LLPS-Scj-1135Schizosaccharomyces japonicus27.896e-37 150
LLPS-Zyt-0047Zymoseptoria tritici27.577e-38 153
LLPS-Abg-0116Absidia glauca27.488e-33 137
LLPS-Pytr-0931Pyrenophora triticirepentis27.382e-47 181
LLPS-Asc-1154Aspergillus clavatus27.248e-49 186
LLPS-Lem-0394Leptosphaeria maculans27.19e-45 173
LLPS-Dos-1044Dothistroma septosporum27.12e-36 149
LLPS-Nef-0657Neosartorya fischeri27.081e-35 146
LLPS-Fus-1073Fusarium solani27.072e-44 172
LLPS-Fuo-1106Fusarium oxysporum26.961e-46 179
LLPS-Fuv-1468Fusarium verticillioides26.763e-45 175
LLPS-Asfu-1486Aspergillus fumigatus26.367e-51 191
LLPS-Pyt-1518Pyrenophora teres26.193e-41 163
LLPS-Ast-0712Aspergillus terreus26.091e-34 143
LLPS-Leo-1379Lepisosteus oculatus26.045e-33 139
LLPS-Cogr-1530Colletotrichum graminicola26.025e-42 166
LLPS-Cog-0555Colletotrichum gloeosporioides25.817e-43 168
LLPS-Anc-3301Anolis carolinensis25.043e-29 127
LLPS-Php-2295Physcomitrella patens24.951e-1378.2
LLPS-Ved-1527Verticillium dahliae24.923e-41 164
LLPS-Tag-2187Taeniopygia guttata24.593e-28 124
LLPS-Crn-0101Cryptococcus neoformans24.235e-22 104
LLPS-Fia-1693Ficedula albicollis24.189e-21 100
LLPS-Osl-0535Ostreococcus lucimarinus23.399e-1168.9
LLPS-Spr-1383Sporisorium reilianum23.187e-30 128
LLPS-Ors-0437Oryza sativa22.483e-0654.3
LLPS-Cae-1041Caenorhabditis elegans21.592e-1583.6