DrLLPS - Data resource of LLPS

LLPS-Sac-0690
TRF5

▼ OVERVIEW


Status:	Reviewed
Protein Name:	Poly(A) RNA polymerase protein 1; Topoisomerase 1-related protein TRF5
Gene Name:	TRF5, YNL299W, N0440
Ensembl Gene:	YNL299W
Ensembl Protein:	YNL299W_mRNA
Organism:	Saccharomyces cerevisiae
Taxa ID:	559292
LLPS Type:	Client

▼ PROPERTY

——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:

Condensate	Description	Tissue/Cell	PMIDs
Nucleolus	"...Out of the 142 yeast nucleolar proteins that have at least one human homologue, 124 are found in the updated nucleolar proteome."	Saccharomyces cerevisiae	15635413

▼ FUNCTION

Catalytic subunit of the TRAMP5 complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates like cryptic transcripts generated by RNA polymerase II and III, or hypomethylated pre-tRNAi-Met. Polyadenylates RNA processing and degradation intermediates of snRNAs, snoRNAs and mRNAs that accumulate in strains lacking a functional exosome. TRF5 is also required for proper nuclear division in mitosis and sister chromatid cohesion. Involved in the regulation of histone mRNA levels. May mediate mitotic chromosome condensation.

▼ CROSS REFERENCE

Database	Nucleotide ID	Protein ID
Ensembl	YNL299W_mRNA	YNL299W_mRNA
UniProt		P48561, TRF5_YEAST, D6W0P5
GeneBank	BK006947, U47282, Z71575, U23084	AAC49397.1, DAA10261.1, CAA96217.1, AAC49099.1
RefSeq	NM_001183137.1	NP_014100.2
Entrez	855417

▼ SEQUENCE

Protein Sequence (FASTA)
1 MTRLKAKYSP TKGKRKEDKH TKRMRKSSFT RTQKMLEVFN DNRSHFNKYE SLAIDVEDDD 60 61 TFGNLVLMEN DKSDVDIPVI EEVTSSEDEQ RAESSKRNNS LEDNQDFIAF SDSSEDETEQ 120 121 IKEDDDERSS FLLTDEHEVS KLTSQQSLNT ESACNVEYPW IRNHCHSKQR RIADWLTSEI 180 181 KDFVHYISPS KNEIKCRNRT IDKLRRAVKE LWSDADLHVF GSFATDLYLP GSDIDCVVNS 240 241 RNRDKEDRNY IYELARHLKN KGLAIRMEVI VKTRVPIIKF IEPQSQLHID VSFERTNGLE 300 301 AAKLIREWLR DSPGLRELVL IIKQFLHSRR LNNVHTGGLG GFTVICLVYS FLNMHPRIKS 360 361 NDIDVLDNLG VLLIDFFELY GKNFGYDDVA ISISDGYASY IPKSCWRTLE PSRSKFSLAI 420 421 QDPGDPNNNI SRGSFNMKDI KKAFAGAFEL LVNKCWELNS ATFKDRVGKS ILGNVIKYRG 480 481 QKRDFNDERD LVQNKAIIEN ERYHKRRTRI VQEDLFINDT EDLPVEEIYK LDEPAKKKQK 540 541 AKKDKREGEI KKSAIPSPPP DFGVSRSKLK RKVKKTDQGS LLHQNNLSID DLMGLSENDQ 600 601 ESDQDQKGRD TPSGQDEKSP LETKTVDAQT RRDYWLSKGQ AL 642
Nucleotide CDS Sequence (FASTA)
1 ATGACAAGGC TCAAAGCAAA ATATTCACCT ACAAAAGGTA AAAGAAAAGA AGATAAACAT 60 61 ACAAAACGTA TGCGTAAATC ATCCTTCACA AGAACGCAAA AAATGCTTGA AGTATTTAAC 120 121 GATAATCGCT CGCATTTTAA CAAGTACGAG AGCCTCGCTA TCGACGTTGA AGACGATGAC 180 181 ACTTTCGGAA ACCTAGTCTT AATGGAAAAT GATAAAAGTG ATGTCGACAT ACCTGTAATA 240 241 GAAGAAGTCA CATCAAGTGA AGATGAACAA AGGGCAGAAT CTAGCAAAAG AAACAATTCT 300 301 CTCGAAGATA ATCAAGATTT TATTGCTTTT TCGGACAGCT CTGAAGATGA AACAGAACAA 360 361 ATAAAGGAAG ATGATGATGA AAGAAGCAGT TTTCTGTTGA CCGATGAACA TGAAGTTTCT 420 421 AAGCTTACTT CACAGCAATC TCTGAACACT GAAAGCGCTT GCAATGTGGA GTATCCTTGG 480 481 ATAAGAAATC ATTGTCATTC GAAACAAAGG AGAATAGCAG ATTGGCTAAC TTCAGAAATC 540 541 AAAGATTTCG TTCATTATAT CTCTCCAAGC AAGAACGAAA TCAAGTGCAG AAATAGAACG 600 601 ATCGATAAAC TACGCCGGGC GGTCAAGGAA CTGTGGTCGG ATGCAGATTT GCATGTGTTT 660 661 GGTTCTTTTG CCACCGATTT ATATTTGCCG GGTTCTGACA TTGACTGTGT CGTAAACAGC 720 721 CGGAATCGCG ATAAAGAGGA CAGAAATTAC ATTTATGAAT TGGCGAGACA TTTGAAAAAC 780 781 AAAGGGCTAG CTATAAGAAT GGAGGTTATT GTTAAAACGC GAGTGCCTAT TATCAAATTC 840 841 ATAGAGCCCC AATCACAGTT ACATATCGAC GTTTCATTTG AAAGAACGAA CGGGTTAGAG 900 901 GCTGCAAAAC TCATCAGGGA ATGGCTAAGA GACTCTCCAG GGTTACGTGA ACTGGTACTG 960 961 ATAATAAAAC AATTTTTGCA TTCAAGACGA TTGAACAATG TGCACACAGG AGGATTGGGA 1020 1021 GGTTTCACAG TAATCTGTTT GGTTTATTCT TTTTTAAATA TGCATCCGCG AATAAAATCA 1080 1081 AACGATATCG ACGTTCTAGA TAACCTGGGT GTTCTACTGA TTGACTTCTT CGAACTTTAC 1140 1141 GGAAAAAATT TTGGTTATGA TGACGTTGCT ATAAGCATAT CTGATGGTTA CGCCTCTTAT 1200 1201 ATTCCTAAAT CGTGTTGGAG AACTTTGGAA CCTTCAAGGA GTAAATTCTC GCTAGCTATT 1260 1261 CAAGATCCTG GTGATCCTAA CAATAATATA AGCAGAGGCT CCTTTAATAT GAAGGATATA 1320 1321 AAAAAGGCCT TTGCTGGTGC CTTCGAACTG TTGGTCAATA AATGCTGGGA ATTGAATTCT 1380 1381 GCTACATTCA AAGACCGCGT TGGAAAAAGT ATACTCGGAA ACGTGATCAA ATACCGTGGG 1440 1441 CAAAAGAGAG ATTTCAACGA CGAAAGAGAT TTAGTGCAGA ATAAAGCCAT CATTGAAAAC 1500 1501 GAGCGCTACC ACAAGAGGCG CACGAGGATA GTTCAAGAAG ATTTATTTAT AAACGACACC 1560 1561 GAAGACTTGC CAGTAGAGGA GATCTACAAA CTTGACGAAC CTGCAAAGAA GAAACAGAAG 1620 1621 GCAAAAAAGG ATAAGAGAGA AGGGGAAATC AAAAAGAGTG CCATTCCATC TCCACCTCCG 1680 1681 GATTTTGGAG TGAGCAGAAG CAAATTGAAA CGCAAAGTGA AAAAAACTGA TCAGGGCTCT 1740 1741 CTCCTACACC AAAACAACCT CAGCATAGAT GACTTGATGG GACTTTCTGA GAATGATCAA 1800 1801 GAATCCGATC AAGATCAAAA AGGAAGAGAT ACCCCTTCGG GACAAGATGA GAAATCACCA 1860 1861 CTGGAAACTA AGACAGTAGA TGCTCAAACG AGAAGGGACT ACTGGCTCTC TAAAGGCCAG 1920 1921 GCTCTTTAA 1929

▼ KEYWORD

ID	Family
KW-0131	Cell cycle
KW-0132	Cell division
KW-0181	Complete proteome
KW-0460	Magnesium
KW-0464	Manganese
KW-0479	Metal-binding
KW-0498	Mitosis
KW-0548	Nucleotidyltransferase
KW-0539	Nucleus
KW-0597	Phosphoprotein
KW-1185	Reference proteome
KW-0808	Transferase

▼ GENE ONTOLOGY

ID	Classification	Description
GO:0005730	Cellular Component	Nucleolus
GO:0031499	Cellular Component	TRAMP complex
GO:0046872	Molecular Function	Metal ion binding
GO:0004652	Molecular Function	Polynucleotide adenylyltransferase activity
GO:0007049	Biological Process	Cell cycle
GO:0051301	Biological Process	Cell division
GO:0071044	Biological Process	Histone mRNA catabolic process
GO:0043629	Biological Process	NcRNA polyadenylation
GO:0071039	Biological Process	Nuclear polyadenylation-dependent CUT catabolic process
GO:0071042	Biological Process	Nuclear polyadenylation-dependent mRNA catabolic process
GO:0071035	Biological Process	Nuclear polyadenylation-dependent rRNA catabolic process
GO:0071036	Biological Process	Nuclear polyadenylation-dependent snoRNA catabolic process
GO:0071037	Biological Process	Nuclear polyadenylation-dependent snRNA catabolic process
GO:0071038	Biological Process	Nuclear polyadenylation-dependent tRNA catabolic process
GO:0071051	Biological Process	Polyadenylation-dependent snoRNA 3'-end processing
GO:0071050	Biological Process	SnoRNA polyadenylation
GO:0034475	Biological Process	U4 snRNA 3'-end processing

▼ KEGG

sce:YNL299W

▼ ANNOTATION

Disorder
IUPred2A

Domain
SMART	Pfam	InterPro	LCR-eXXXplorer	CDD
Gene3D

PTM
EPSD	PLMD	dbPTM

Interaction
HINT	Mentha	PINA	IID	iRefIndex
MIST

Physicochemical
Compute pI/Mw	AAindex

Localization
NLSdb

▼ ORTHOLOGY

DrLLPS ID	Organism	Identity	E-value	Score
LLPS-Asg-0154	Ashbya gossypii	51.66	0.0	602
LLPS-Kop-0879	Komagataella pastoris	50.46	3e-146	445
LLPS-Yal-1418	Yarrowia lipolytica	50.3	5e-110	354
LLPS-Drm-1571	Drosophila melanogaster	38.41	4e-53	201
LLPS-Tum-0328	Tuber melanosporum	38.22	3e-58	214
LLPS-Tra-2645	Triticum aestivum	38.19	2e-53	198
LLPS-Gaa-1130	Gasterosteus aculeatus	38.11	1e-53	198
LLPS-Scj-0840	Schizosaccharomyces japonicus	37.99	9e-69	242
LLPS-Tar-3066	Takifugu rubripes	37.99	4e-52	197
LLPS-Abg-1908	Absidia glauca	37.88	5e-56	205
LLPS-Hov-0395	Hordeum vulgare	37.74	6e-54	197
LLPS-Xim-1351	Xiphophorus maculatus	37.7	6e-58	214
LLPS-Pof-0128	Poecilia formosa	37.65	1e-53	200
LLPS-Crn-1107	Cryptococcus neoformans	37.61	5e-68	242
LLPS-Caj-2378	Callithrix jacchus	37.58	1e-51	194
LLPS-Gog-3177	Gorilla gorilla	37.58	1e-51	194
LLPS-Sus-2181	Sus scrofa	37.58	1e-51	194
LLPS-Cea-0185	Cercocebus atys	37.58	2e-51	194
LLPS-Rhb-2562	Rhinopithecus bieti	37.58	1e-51	194
LLPS-Man-1459	Macaca nemestrina	37.58	1e-51	194
LLPS-Hos-2274	Homo sapiens	37.58	1e-51	194
LLPS-Pat-0681	Pan troglodytes	37.58	1e-51	194
LLPS-Nol-1671	Nomascus leucogenys	37.58	2e-51	194
LLPS-Caf-1542	Canis familiaris	37.58	1e-51	194
LLPS-Brd-0455	Brachypodium distachyon	37.54	2e-52	196
LLPS-Blg-0285	Blumeria graminis	37.5	4e-50	191
LLPS-Orbr-0128	Oryza brachyantha	37.42	3e-56	203
LLPS-Usm-0377	Ustilago maydis	37.39	5e-65	233
LLPS-Pes-3105	Pelodiscus sinensis	37.38	4e-54	198
LLPS-Scm-0854	Scophthalmus maximus	37.28	2e-49	186
LLPS-Mel-0780	Melampsora laricipopulina	37.25	2e-60	223
LLPS-Sem-1573	Selaginella moellendorffii	37.17	3e-51	185
LLPS-Eqc-3362	Equus caballus	37.05	3e-50	191
LLPS-Mao-1620	Magnaporthe oryzae	36.95	2e-54	202
LLPS-Lac-0405	Latimeria chalumnae	36.76	1e-53	200
LLPS-Sot-0519	Solanum tuberosum	36.73	8e-57	206
LLPS-Osl-0600	Ostreococcus lucimarinus	36.72	2e-53	192
LLPS-Aon-0346	Aotus nancymaae	36.71	1e-51	194
LLPS-Mum-2041	Mus musculus	36.71	1e-51	194
LLPS-Mea-0592	Mesocricetus auratus	36.71	2e-51	194
LLPS-Ova-1316	Ovis aries	36.71	6e-53	195
LLPS-Aim-0179	Ailuropoda melanoleuca	36.71	9e-52	194
LLPS-Loa-1829	Loxodonta africana	36.71	4e-53	196
LLPS-Pap-1066	Pan paniscus	36.71	5e-52	194
LLPS-Cap-0232	Cavia porcellus	36.71	2e-51	194
LLPS-Otg-2235	Otolemur garnettii	36.69	5e-52	195
LLPS-Ran-1453	Rattus norvegicus	36.65	2e-52	195
LLPS-Gas-0148	Galdieria sulphuraria	36.65	3e-47	177
LLPS-Hea-2555	Helianthus annuus	36.62	4e-58	210
LLPS-Mod-0508	Monodelphis domestica	36.59	4e-53	197
LLPS-Orl-0642	Oryzias latipes	36.57	1e-53	200
LLPS-Scf-0117	Scleropages formosus	36.56	4e-50	186
LLPS-Php-2092	Physcomitrella patens	36.54	3e-52	196
LLPS-Bot-3272	Bos taurus	36.51	6e-52	195
LLPS-Fec-0059	Felis catus	36.51	8e-52	195
LLPS-Ora-0363	Ornithorhynchus anatinus	36.45	3e-54	197
LLPS-Mal-1625	Mandrillus leucophaeus	36.39	3e-52	194
LLPS-Amt-2250	Amborella trichopoda	36.39	8e-52	192
LLPS-Poa-2152	Pongo abelii	36.39	8e-53	194
LLPS-Myl-0190	Myotis lucifugus	36.36	2e-37	149
LLPS-Mua-0091	Musa acuminata	36.28	1e-53	197
LLPS-Dar-2639	Danio rerio	36.28	1e-53	199
LLPS-Ten-2491	Tetraodon nigroviridis	36.27	4e-34	139
LLPS-Dac-1578	Daucus carota	36.25	4e-53	196
LLPS-Orn-1118	Oreochromis niloticus	36.22	3e-53	197
LLPS-Fud-0581	Fukomys damarensis	36.19	2e-52	194
LLPS-Orc-0248	Oryctolagus cuniculus	36.19	3e-53	195
LLPS-Fia-2083	Ficedula albicollis	36.19	2e-53	196
LLPS-Anp-0502	Anas platyrhynchos	36.19	6e-54	198
LLPS-Meg-1291	Meleagris gallopavo	36.19	4e-54	198
LLPS-Tag-1874	Taeniopygia guttata	36.19	2e-53	196
LLPS-Gaga-1675	Gallus gallus	36.19	1e-52	197
LLPS-Gag-0687	Gaeumannomyces graminis	36.18	4e-58	214
LLPS-Sol-0131	Solanum lycopersicum	36.16	3e-57	211
LLPS-Nec-1505	Neurospora crassa	36.16	1e-55	207
LLPS-Miv-1244	Microbotryum violaceum	36.11	2e-25	115
LLPS-Dio-1925	Dipodomys ordii	36.08	1e-52	195
LLPS-Urm-2170	Ursus maritimus	36.06	4e-53	197
LLPS-Icp-0648	Ictalurus punctatus	35.87	1e-50	191
LLPS-Leo-0117	Lepisosteus oculatus	35.87	8e-52	194
LLPS-Tut-2237	Tursiops truncatus	35.87	2e-51	190
LLPS-Paa-1093	Papio anubis	35.86	2e-52	197
LLPS-Nef-1182	Neosartorya fischeri	35.81	4e-47	181
LLPS-Asfu-1020	Aspergillus fumigatus	35.81	5e-48	183
LLPS-Brn-0412	Brassica napus	35.71	2e-52	194
LLPS-Brr-2760	Brassica rapa	35.71	2e-52	194
LLPS-Bro-1965	Brassica oleracea	35.71	2e-52	194
LLPS-Asm-1643	Astyanax mexicanus	35.56	1e-49	188
LLPS-Sob-1569	Sorghum bicolor	35.48	4e-54	199
LLPS-Nia-0085	Nicotiana attenuata	35.47	2e-56	205
LLPS-Map-1229	Magnaporthe poae	35.45	2e-54	203
LLPS-Mae-1751	Manihot esculenta	35.4	2e-51	191
LLPS-Prp-1359	Prunus persica	35.28	1e-55	203
LLPS-Mam-3402	Macaca mulatta	35.26	8e-53	198
LLPS-Zyt-0724	Zymoseptoria tritici	35.24	3e-44	171
LLPS-Thc-0858	Theobroma cacao	35.22	8e-50	187
LLPS-Mup-0940	Mustela putorius furo	35.22	1e-51	192
LLPS-Pot-0770	Populus trichocarpa	35.22	2e-46	177
LLPS-Art-2976	Arabidopsis thaliana	35.22	1e-49	186
LLPS-Asni-0638	Aspergillus niger	35.2	3e-47	180
LLPS-Trv-1301	Trichoderma virens	35.19	6e-51	192
LLPS-Xet-0344	Xenopus tropicalis	35.18	2e-43	168
LLPS-Aso-0343	Aspergillus oryzae	35.09	2e-43	165
LLPS-Via-0777	Vigna angularis	35.09	2e-47	179
LLPS-Anc-0889	Anolis carolinensis	34.94	8e-54	200
LLPS-Scc-1045	Schizosaccharomyces cryophilus	34.91	6e-60	218
LLPS-Chs-0877	Chlorocebus sabaeus	34.88	2e-51	191
LLPS-Viv-0774	Vitis vinifera	34.88	2e-56	204
LLPS-Ict-1996	Ictidomys tridecemlineatus	34.88	3e-51	191
LLPS-Cas-0375	Carlito syrichta	34.88	3e-51	191
LLPS-Asn-0034	Aspergillus nidulans	34.86	6e-51	192
LLPS-Cus-1639	Cucumis sativus	34.82	2e-55	202
LLPS-Scs-0150	Sclerotinia sclerotiorum	34.81	3e-49	187
LLPS-Maf-4393	Macaca fascicularis	34.8	3e-43	169
LLPS-Phv-1453	Phaseolus vulgaris	34.78	8e-48	181
LLPS-Cii-1006	Ciona intestinalis	34.74	9e-51	186
LLPS-Fuv-1209	Fusarium verticillioides	34.74	4e-51	192
LLPS-Fuo-1366	Fusarium oxysporum	34.74	4e-51	192
LLPS-Lep-0699	Leersia perrieri	34.68	1e-54	201
LLPS-Arl-2022	Arabidopsis lyrata	34.59	7e-49	184
LLPS-Scp-1416	Schizosaccharomyces pombe	34.54	1e-68	243
LLPS-Cis-1810	Ciona savignyi	34.47	4e-47	181
LLPS-Asc-1117	Aspergillus clavatus	34.44	2e-45	176
LLPS-Asf-0138	Aspergillus flavus	34.44	1e-44	173
LLPS-Sah-1583	Sarcophilus harrisii	34.26	4e-52	194
LLPS-Met-1238	Medicago truncatula	34.25	3e-52	193
LLPS-Ast-0186	Aspergillus terreus	34.25	1e-46	179
LLPS-Coo-1171	Colletotrichum orbiculare	34.2	3e-47	181
LLPS-Fus-0732	Fusarium solani	33.89	2e-49	187
LLPS-Trr-1352	Trichoderma reesei	33.88	1e-51	194
LLPS-Spr-0753	Sporisorium reilianum	33.74	3e-48	184
LLPS-Gor-1870	Gossypium raimondii	33.65	6e-49	184
LLPS-Pug-1083	Puccinia graminis	33.51	2e-46	180
LLPS-Sei-1625	Setaria italica	33.42	4e-54	199
LLPS-Zem-1190	Zea mays	33.42	5e-52	194
LLPS-Cogr-1548	Colletotrichum graminicola	33.33	1e-45	177
LLPS-Beb-0450	Beauveria bassiana	32.35	2e-43	169
LLPS-Chc-0424	Chondrus crispus	32.25	4e-43	171
LLPS-Dos-1053	Dothistroma septosporum	32.13	3e-41	164
LLPS-Cae-0277	Caenorhabditis elegans	31.92	6e-42	166
LLPS-Coc-0260	Corchorus capsularis	31.89	4e-36	149
LLPS-Ved-1305	Verticillium dahliae	31.47	9e-35	144
LLPS-Phn-1339	Phaeosphaeria nodorum	31.06	7e-42	165
LLPS-Orp-0320	Oryza punctata	30.79	1e-51	193
LLPS-Vir-1711	Vigna radiata	30.43	2e-26	118
LLPS-Chr-1509	Chlamydomonas reinhardtii	30.43	3e-40	162
LLPS-Ors-0976	Oryza sativa	30.06	1e-55	204
LLPS-Ori-0115	Oryza indica	29.84	4e-52	194
LLPS-Orb-0477	Oryza barthii	29.75	2e-51	192
LLPS-Orm-0500	Oryza meridionalis	29.75	1e-51	193
LLPS-Pytr-0658	Pyrenophora triticirepentis	29.72	1e-39	157
LLPS-Orni-1780	Oryza nivara	29.7	3e-52	195
LLPS-Orgl-1786	Oryza glumaepatula	29.64	5e-52	194
LLPS-Glm-2279	Glycine max	29.62	4e-45	173
LLPS-Orr-0419	Oryza rufipogon	29.54	1e-50	190
LLPS-Put-0233	Puccinia triticina	28.8	4e-29	127
LLPS-Pyt-0384	Pyrenophora teres	28.34	7e-40	159
LLPS-Cog-1504	Colletotrichum gloeosporioides	26.77	2e-17	91.3
LLPS-Lem-0518	Leptosphaeria maculans	26.5	4e-39	157

LLPS-Sac-0690TRF5

Protein Sequence

Nucleotide Sequence

LLPS-Sac-0690
TRF5