LLPS-Sac-0506
BUD23

▼ OVERVIEW

Status: Reviewed
Protein Name: 18S rRNA (guanine(1575)-N(7))-methyltransferase; Bud site selection protein 23
Gene Name: BUD23, YCR047C, YCR47C
Ensembl Gene: YCR047C
Ensembl Protein: YCR047C_mRNA
Organism: Saccharomyces cerevisiae
Taxa ID: 559292
LLPS Type: Others
PDB: 4QTU (A, B) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Nucleolus
"...Out of the 142 yeast nucleolar proteins that have at least one human homologue, 124 are found in the updated nucleolar proteome."
Saccharomyces cerevisiae15635413

▼ FUNCTION

S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the N(7) position of guanine 1575 (m7G1575) in 18S rRNA. Requires the methyltransferase adapter protein TRM112 for full rRNA methyltransferase activity. Important for biogenesis end export of the 40S ribosomal subunit independent on its methyltransferase activity. Required for efficient cleavage of the primary 35S precursor rRNA at site A2. Involved in positioning the proximal bud pole signal.

▼ CROSS REFERENCE

▼ SEQUENCE

Protein Sequence (FASTA)
1     MSRPEELAPP  EIFYNDSEAH  KYTGSTRVQH  IQAKMTLRAL  ELLNLQPCSF  ILDIGCGSGL  60
61    SGEILTQEGD  HVWCGLDISP  SMLATGLSRE  LEGDLMLQDM  GTGIPFRAGS  FDAAISISAI  120
121   QWLCNADTSY  NDPKQRLMRF  FNTLYAALKK  GGKFVAQFYP  KNDDQVDDIL  QSAKVAGFSG  180
181   GLVVDDPESK  KNKKYYLVLS  SGAPPQGEEQ  VNLDGVTMDE  ENVNLKKQLR  QRLKGGKDKE  240
241   SAKSFILRKK  ELMKRRGRKV  AKDSKFTGRK  RRHRF  275
Nucleotide CDS Sequence (FASTA)
1     ATGTCACGTC  CTGAGGAGTT  GGCACCACCG  GAGATTTTCT  ATAATGATAG  CGAAGCACAC  60
61    AAGTACACGG  GTTCGACCAG  AGTGCAGCAT  ATCCAGGCGA  AGATGACGCT  GAGGGCGTTG  120
121   GAGCTTTTGA  ATCTGCAGCC  GTGCAGTTTC  ATTCTGGATA  TCGGGTGCGG  GTCCGGACTG  180
181   TCTGGGGAGA  TTTTGACGCA  GGAGGGAGAC  CATGTGTGGT  GTGGTTTGGA  TATATCGCCC  240
241   AGCATGCTTG  CGACCGGTCT  TAGTAGAGAG  CTGGAGGGCG  ACTTGATGTT  GCAGGATATG  300
301   GGCACCGGGA  TACCGTTCCG  GGCGGGCTCG  TTTGACGCGG  CTATTAGTAT  CAGTGCGATC  360
361   CAATGGCTGT  GCAATGCGGA  CACTTCATAC  AACGATCCTA  AACAGCGGTT  GATGAGGTTT  420
421   TTCAACACAT  TGTATGCTGC  ACTGAAGAAG  GGAGGGAAAT  TTGTGGCCCA  GTTCTACCCG  480
481   AAAAACGACG  ACCAGGTGGA  CGACATACTG  CAGTCTGCCA  AGGTGGCAGG  GTTCAGTGGC  540
541   GGGCTTGTGG  TGGACGACCC  AGAGTCTAAA  AAGAATAAGA  AGTACTACCT  TGTGTTGAGC  600
601   AGTGGGGCCC  CACCGCAGGG  GGAGGAGCAG  GTGAATTTGG  ACGGTGTGAC  CATGGACGAG  660
661   GAGAACGTCA  ACTTGAAGAA  ACAACTGCGC  CAGCGCTTGA  AGGGAGGCAA  AGACAAGGAG  720
721   TCTGCCAAGA  GTTTCATTCT  AAGAAAGAAG  GAGCTCATGA  AAAGACGTGG  GAGGAAAGTT  780
781   GCGAAGGACT  CCAAGTTCAC  CGGGAGGAAA  AGAAGACACA  GGTTCTAG  828

▼ KEYWORD

ID
Family
3D-structure
Complete proteome
Cytoplasm
Methyltransferase
Nucleus
Reference proteome
rRNA processing
S-adenosyl-L-methionine
Transferase

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cytoplasm
Cellular Component
Nucleolus
Cellular Component
Nucleus
Molecular Function
RRNA (guanine) methyltransferase activity
Molecular Function
S-adenosylmethionine-dependent methyltransferase activity
Biological Process
Cellular bud site selection
Biological Process
Endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Biological Process
Ribosomal small subunit export from nucleus
Biological Process
RRNA (guanine-N7)-methylation

▼ KEGG


▼ ANNOTATION

Disorder
IUPred2A
Physicochemical
Compute pI/MwAAindex
Function
PIRSF
Structure
PDB
Localization
NLSdb

▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Asg-0720Ashbya gossypii77.821e-156 445
LLPS-Kop-0286Komagataella pastoris68.681e-133 386
LLPS-Yal-1016Yarrowia lipolytica66.916e-130 377
LLPS-Fuv-0559Fusarium verticillioides62.31e-75 236
LLPS-Dos-1049Dothistroma septosporum56.324e-101 303
LLPS-Ved-0664Verticillium dahliae55.972e-83 258
LLPS-Eqc-0409Equus caballus55.696e-57 192
LLPS-Nec-0158Neurospora crassa55.444e-97 293
LLPS-Cogr-0172Colletotrichum graminicola55.283e-100 301
LLPS-Blg-0084Blumeria graminis54.842e-97 294
LLPS-Mao-0423Magnaporthe oryzae54.87e-94 285
LLPS-Cog-0513Colletotrichum gloeosporioides54.778e-99 298
LLPS-Abg-0866Absidia glauca54.525e-93 283
LLPS-Scp-0274Schizosaccharomyces pombe54.512e-99 299
LLPS-Coo-1097Colletotrichum orbiculare54.421e-99 300
LLPS-Asc-0309Aspergillus clavatus54.425e-98 296
LLPS-Gag-0130Gaeumannomyces graminis54.268e-100 300
LLPS-Scs-0692Sclerotinia sclerotiorum54.265e-96 291
LLPS-Spr-0080Sporisorium reilianum54.181e-96 293
LLPS-Trv-0156Trichoderma virens53.96e-97 293
LLPS-Tum-0512Tuber melanosporum53.821e-82 260
LLPS-Ast-0168Aspergillus terreus53.716e-96 290
LLPS-Scj-0090Schizosaccharomyces japonicus53.625e-96 290
LLPS-Lem-0151Leptosphaeria maculans53.521e-97 295
LLPS-Pyt-1176Pyrenophora teres53.527e-92 280
LLPS-Pytr-0026Pyrenophora triticirepentis53.527e-92 280
LLPS-Asn-0985Aspergillus nidulans53.362e-95 289
LLPS-Fuo-0763Fusarium oxysporum53.211e-93 284
LLPS-Trr-0928Trichoderma reesei53.197e-96 290
LLPS-Fus-0613Fusarium solani53.193e-96 291
LLPS-Pug-1452Puccinia graminis53.028e-96 291
LLPS-Asf-0153Aspergillus flavus53.012e-79 247
LLPS-Asfu-0750Aspergillus fumigatus53.011e-78 245
LLPS-Aso-0084Aspergillus oryzae53.01e-96 292
LLPS-Asni-0557Aspergillus niger53.06e-95 288
LLPS-Beb-1169Beauveria bassiana53.02e-94 287
LLPS-Crn-0141Cryptococcus neoformans52.926e-93 283
LLPS-Zyt-0440Zymoseptoria tritici52.865e-95 288
LLPS-Vir-0626Vigna radiata52.851e-54 183
LLPS-Nef-0628Neosartorya fischeri52.211e-77 242
LLPS-Amt-0274Amborella trichopoda52.035e-73 233
LLPS-Gas-0112Galdieria sulphuraria52.011e-89 274
LLPS-Scc-0424Schizosaccharomyces cryophilus51.998e-86 264
LLPS-Usm-0387Ustilago maydis51.975e-95 289
LLPS-Lac-2451Latimeria chalumnae51.797e-88 270
LLPS-Phn-0015Phaeosphaeria nodorum51.582e-83 258
LLPS-Phv-0052Phaseolus vulgaris51.384e-88 271
LLPS-Miv-1018Microbotryum violaceum51.343e-93 284
LLPS-Scf-1458Scleropages formosus51.068e-85 265
LLPS-Via-0080Vigna angularis51.036e-86 265
LLPS-Thc-1219Theobroma cacao51.039e-86 265
LLPS-Nia-0268Nicotiana attenuata51.038e-85 263
LLPS-Leo-2220Lepisosteus oculatus50.716e-86 265
LLPS-Icp-0745Ictalurus punctatus50.71e-81 254
LLPS-Mae-0865Manihot esculenta50.691e-80 252
LLPS-Php-0156Physcomitrella patens50.685e-88 271
LLPS-Sol-0367Solanum lycopersicum50.681e-84 262
LLPS-Mel-1090Melampsora laricipopulina50.659e-95 288
LLPS-Arl-0211Arabidopsis lyrata50.525e-85 263
LLPS-Art-0711Arabidopsis thaliana50.524e-86 266
LLPS-Drm-0558Drosophila melanogaster50.184e-88 270
LLPS-Orl-0439Oryzias latipes50.189e-84 259
LLPS-Brn-0110Brassica napus50.174e-84 260
LLPS-Bro-0098Brassica oleracea50.174e-84 260
LLPS-Glm-2061Glycine max50.07e-85 263
LLPS-Anc-0396Anolis carolinensis50.01e-86 267
LLPS-Cus-0958Cucumis sativus49.837e-86 265
LLPS-Sot-0176Solanum tuberosum49.834e-83 258
LLPS-Met-0067Medicago truncatula49.824e-85 263
LLPS-Mea-0178Mesocricetus auratus49.824e-84 260
LLPS-Gaga-2020Gallus gallus49.826e-90 275
LLPS-Dar-1954Danio rerio49.828e-88 270
LLPS-Viv-0027Vitis vinifera49.664e-82 256
LLPS-Pot-0751Populus trichocarpa49.668e-85 262
LLPS-Tar-1626Takifugu rubripes49.622e-79 249
LLPS-Zem-0017Zea mays49.52e-83 259
LLPS-Brr-1036Brassica rapa49.494e-81 257
LLPS-Fud-0080Fukomys damarensis49.467e-85 262
LLPS-Cap-2705Cavia porcellus49.462e-83 259
LLPS-Gor-0457Gossypium raimondii49.312e-82 256
LLPS-Pat-2980Pan troglodytes49.298e-83 257
LLPS-Fia-0355Ficedula albicollis49.236e-76 239
LLPS-Mum-0485Mus musculus49.123e-83 258
LLPS-Scm-0437Scophthalmus maximus49.121e-81 254
LLPS-Ten-1568Tetraodon nigroviridis49.113e-82 255
LLPS-Orn-1013Oreochromis niloticus49.13e-82 256
LLPS-Mod-0820Monodelphis domestica49.14e-87 268
LLPS-Orgl-0870Oryza glumaepatula49.012e-81 258
LLPS-Ori-0724Oryza indica49.014e-83 258
LLPS-Orm-0188Oryza meridionalis49.012e-81 258
LLPS-Orr-1026Oryza rufipogon49.011e-81 258
LLPS-Orbr-0352Oryza brachyantha49.017e-82 255
LLPS-Ors-1179Oryza sativa49.014e-83 258
LLPS-Orni-0422Oryza nivara49.04e-82 259
LLPS-Orp-0272Oryza punctata49.06e-82 255
LLPS-Brd-0749Brachypodium distachyon48.994e-83 258
LLPS-Tra-1755Triticum aestivum48.981e-81 254
LLPS-Orb-1326Oryza barthii48.844e-82 259
LLPS-Org-1751Oryza glaberrima48.842e-83 259
LLPS-Sem-0850Selaginella moellendorffii48.812e-86 266
LLPS-Xet-0319Xenopus tropicalis48.771e-86 267
LLPS-Ran-2849Rattus norvegicus48.762e-83 258
LLPS-Xim-0111Xiphophorus maculatus48.765e-85 263
LLPS-Pof-0207Poecilia formosa48.764e-85 263
LLPS-Dac-0123Daucus carota48.757e-74 243
LLPS-Ict-1919Ictidomys tridecemlineatus48.751e-84 261
LLPS-Tag-1393Taeniopygia guttata48.75e-83 257
LLPS-Cas-0502Carlito syrichta48.573e-81 253
LLPS-Mup-3022Mustela putorius furo48.475e-75 236
LLPS-Poa-1719Pongo abelii48.41e-81 254
LLPS-Urm-1505Ursus maritimus48.396e-81 252
LLPS-Hov-0393Hordeum vulgare48.35e-80 251
LLPS-Osl-0584Ostreococcus lucimarinus48.083e-82 256
LLPS-Asm-0825Astyanax mexicanus48.064e-86 265
LLPS-Sei-0398Setaria italica48.01e-81 254
LLPS-Sob-0856Sorghum bicolor48.05e-81 253
LLPS-Pes-0929Pelodiscus sinensis47.812e-70 224
LLPS-Chr-0132Chlamydomonas reinhardtii47.71e-84 262
LLPS-Lep-0495Leersia perrieri47.74e-75 240
LLPS-Cii-0049Ciona intestinalis47.696e-82 255
LLPS-Chs-2865Chlorocebus sabaeus47.675e-82 255
LLPS-Otg-1010Otolemur garnettii47.679e-82 254
LLPS-Orc-1745Oryctolagus cuniculus47.519e-72 229
LLPS-Caf-0528Canis familiaris47.53e-78 248
LLPS-Meg-1338Meleagris gallopavo47.428e-54 179
LLPS-Gaa-1036Gasterosteus aculeatus47.352e-80 251
LLPS-Myl-0905Myotis lucifugus47.311e-79 249
LLPS-Fec-1729Felis catus47.315e-80 249
LLPS-Mua-0428Musa acuminata47.285e-80 250
LLPS-Prp-0262Prunus persica47.241e-77 244
LLPS-Aim-2014Ailuropoda melanoleuca46.982e-77 243
LLPS-Bot-1080Bos taurus46.955e-81 252
LLPS-Hea-1176Helianthus annuus46.95e-78 245
LLPS-Dio-0365Dipodomys ordii46.792e-79 248
LLPS-Coc-0410Corchorus capsularis46.766e-78 245
LLPS-Mal-0558Mandrillus leucophaeus46.68e-80 250
LLPS-Anp-0938Anas platyrhynchos46.464e-71 227
LLPS-Loa-0082Loxodonta africana46.431e-81 254
LLPS-Caj-0763Callithrix jacchus46.372e-81 254
LLPS-Aon-1470Aotus nancymaae46.282e-79 249
LLPS-Chc-0734Chondrus crispus46.183e-74 235
LLPS-Pap-2387Pan paniscus46.134e-80 251
LLPS-Paa-0020Papio anubis46.089e-80 249
LLPS-Maf-1692Macaca fascicularis46.089e-80 249
LLPS-Man-0097Macaca nemestrina46.089e-80 249
LLPS-Ova-0579Ovis aries46.042e-77 243
LLPS-Cis-0621Ciona savignyi46.044e-78 245
LLPS-Ora-0372Ornithorhynchus anatinus45.782e-38 139
LLPS-Hos-1207Homo sapiens45.762e-80 251
LLPS-Cea-1679Cercocebus atys45.736e-79 248
LLPS-Sus-1148Sus scrofa45.641e-81 254
LLPS-Gog-1531Gorilla gorilla45.056e-77 243
LLPS-Cae-0760Caenorhabditis elegans44.843e-73 232
LLPS-Mam-2940Macaca mulatta44.181e-71 229
LLPS-Rhb-1857Rhinopithecus bieti43.883e-65 212
LLPS-Sah-2869Sarcophilus harrisii42.312e-57 191
LLPS-Cym-0296Cyanidioschyzon merolae40.328e-72 230
LLPS-Nol-0206Nomascus leucogenys36.593e-48 167