LLPS-Ran-2027
Padi4

▼ OVERVIEW

Status: Unreviewed
Protein Name: Protein-arginine deiminase type-4; PAD-R4; Peptidylarginine deiminase IV; Peptidylarginine deiminase type alpha; Protein-arginine deiminase type IV
Gene Name: Padi4, Pad4, Pdi4
Ensembl Gene: ENSRNOG00000006855.2
Ensembl Protein: ENSRNOP00000009081.1
Organism: Rattus norvegicus
Taxa ID: 10116
LLPS Type: Others


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
Stress granulePredicted from orthologs(View)

▼ FUNCTION

Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1 (By similarity).

▼ CROSS REFERENCE

▼ SEQUENCE

Protein Sequence (FASTA)
1     MAQGAVIHVA  PEEPTHAVCV  VGTATPLDIR  GSAPRGSTSF  SITASPEVVV  DVIHGPPSKK  60
61    STTGASKWPL  DPKLEVTLQM  KAASSRIDDQ  KVRISYYGPK  TSSTQALLYL  TGVELSLSAD  120
121   VTRTGKAKPA  PAGKDQSTWT  WGPDGHGAIL  LVNCDKEDPK  SSGMDFEDDK  VLDNKDLQDM  180
181   SPMTLSTKTP  KDFFDKYQLV  LQVPKAKMNK  VRVFRATRGK  LPSRYKVVLG  PQQFSHRLEL  240
241   LGGQHSTDFY  VEGLAFPDAD  FKGLIPLTIS  LLDKSNPELP  EALVFQDTVM  FRVAPWIMTP  300
301   NTQPPQEVYV  CRFSDNEDFL  KSLATFTKKA  KCKLTVCPEE  ENQDDQWMQD  EMEIGYIQAP  360
361   HKTLPVVFDS  PRDRGLKDFP  VKRVMGPNFG  YVTRGLYRAE  VTGLDAFGNL  EVSPPVTVRG  420
421   KEYPLGRILI  GSSGYSSSES  RDMHQILQDF  LGAQQVQAPV  RLFSDWLFVG  HVDEFLSFVP  480
481   ARGKQGFRLL  LSSPRACYQM  FQELQTEGHG  EASLFEGLKR  KRQTISDILS  SQKLRDQNAY  540
541   VESCIDWNRE  VLKRELGLTE  GDIIDIPQLF  RIVGNSRGNP  KAEAFFPNMV  NMLVLGKHLG  600
601   IPKPFGPIIN  GRCCLEEKVC  SLLEPLGLHC  TFINDFYSYH  MYHGEVHCGT  NVRRKPFAFK  660
661   WWHMVP  666
Nucleotide CDS Sequence (FASTA)
1     ATGGCCCAGG  GTGCGGTGAT  CCATGTGGCC  CCTGAGGAGC  CCACCCATGC  CGTGTGTGTG  60
61    GTGGGCACGG  CGACCCCGCT  GGATATCCGT  GGCTCTGCTC  CAAGGGGCTC  CACGTCCTTC  120
121   AGCATCACAG  CCTCCCCAGA  AGTTGTTGTA  GATGTCATCC  ATGGTCCTCC  ATCCAAGAAG  180
181   AGTACCACGG  GGGCCTCCAA  ATGGCCTCTG  GACCCCAAGC  TGGAGGTGAC  CCTACAGATG  240
241   AAAGCAGCCA  GCAGTAGGAT  AGATGACCAG  AAGGTTCGAA  TTTCCTATTA  CGGACCCAAG  300
301   ACCTCATCAA  CCCAAGCCCT  GCTCTACCTC  ACCGGGGTGG  AACTCTCCCT  GAGTGCGGAT  360
361   GTCACCCGCA  CTGGCAAAGC  GAAGCCAGCC  CCAGCTGGGA  AGGATCAGAG  CACCTGGACC  420
421   TGGGGCCCGG  ACGGTCACGG  CGCCATCCTG  TTGGTGAACT  GTGACAAGGA  GGATCCCAAG  480
481   TCCTCCGGAA  TGGACTTCGA  GGACGACAAG  GTCTTAGACA  ACAAAGACCT  GCAGGACATG  540
541   TCACCAATGA  CCCTAAGCAC  GAAGACTCCC  AAAGACTTCT  TTGACAAGTA  TCAGTTGGTG  600
601   CTGCAGGTGC  CGAAGGCCAA  GATGAACAAA  GTGAGAGTCT  TCCGGGCCAC  ACGGGGCAAA  660
661   CTGCCATCCA  GATACAAGGT  GGTCCTAGGA  CCACAGCAGT  TCTCCCACCG  CCTAGAGCTG  720
721   CTGGGCGGCC  AGCACAGCAC  GGACTTCTAT  GTGGAAGGCC  TTGCTTTCCC  TGACGCCGAC  780
781   TTCAAGGGGC  TCATTCCCCT  AACGATCTCC  CTGTTGGACA  AATCTAACCC  GGAGCTCCCT  840
841   GAAGCCCTGG  TGTTCCAAGA  CACCGTGATG  TTCCGTGTGG  CCCCCTGGAT  CATGACGCCC  900
901   AACACTCAGC  CCCCCCAGGA  GGTATACGTG  TGCAGGTTTT  CCGACAATGA  AGACTTCCTA  960
961   AAGTCACTGG  CTACTTTCAC  CAAGAAAGCC  AAGTGCAAAC  TGACTGTGTG  CCCAGAGGAG  1020
1021  GAAAATCAAG  ATGACCAGTG  GATGCAGGAT  GAAATGGAGA  TTGGCTACAT  ACAGGCCCCC  1080
1081  CACAAGACGC  TGCCTGTGGT  CTTTGACTCC  CCGAGGGACA  GAGGCCTGAA  GGATTTCCCT  1140
1141  GTCAAACGAG  TCATGGGTCC  AAATTTTGGC  TATGTGACCC  GAGGGCTCTA  TAGAGCAGAG  1200
1201  GTCACTGGGC  TGGATGCCTT  TGGGAACCTG  GAGGTGAGTC  CCCCAGTCAC  TGTCAGAGGG  1260
1261  AAGGAATACC  CACTGGGCAG  GATTCTCATC  GGGAGCAGCG  GTTATTCCAG  CAGTGAGAGC  1320
1321  CGGGACATGC  ACCAGATCCT  GCAGGACTTC  CTGGGTGCCC  AGCAGGTGCA  GGCCCCTGTG  1380
1381  AGACTCTTCT  CCGATTGGCT  CTTTGTGGGT  CACGTGGATG  AGTTCTTGAG  CTTCGTCCCG  1440
1441  GCACGGGGCA  AGCAGGGCTT  TCGGCTGCTA  CTGTCCAGCC  CCAGAGCTTG  CTACCAGATG  1500
1501  TTCCAGGAGC  TGCAGACCGA  GGGCCACGGG  GAGGCATCAC  TGTTCGAAGG  ACTCAAGAGG  1560
1561  AAAAGGCAGA  CAATCAGTGA  CATTCTGTCC  AGCCAGAAAC  TAAGAGACCA  GAATGCCTAC  1620
1621  GTGGAGAGCT  GTATTGACTG  GAACCGTGAG  GTGCTGAAGC  GAGAGTTGGG  CCTGACGGAG  1680
1681  GGCGACATCA  TTGATATACC  CCAGCTCTTC  AGGATTGTGG  GAAACTCCAG  AGGGAACCCG  1740
1741  AAGGCTGAGG  CCTTCTTCCC  AAACATGGTG  AACATGCTGG  TACTGGGCAA  GCACCTGGGC  1800
1801  ATCCCCAAGC  CCTTTGGGCC  CATCATCAAC  GGCCGCTGCT  GCCTGGAGGA  GAAGGTGTGC  1860
1861  TCCCTGCTGG  AGCCGCTGGG  TCTGCACTGC  ACCTTCATCA  ACGACTTCTA  CAGCTATCAC  1920
1921  ATGTACCACG  GAGAGGTTCA  CTGTGGCACC  AACGTGCGCA  GGAAGCCCTT  CGCCTTCAAG  1980
1981  TGGTGGCACA  TGGTTCCCTG  A  2001

▼ KEYWORD

ID
Family
Calcium
Chromatin regulator
Citrullination
Complete proteome
Cytoplasm
Hydrolase
Immunity
Innate immunity
Metal-binding
Nucleus
Reference proteome
Transcription
Transcription regulation

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cytoplasm
Cellular Component
Nucleus
Cellular Component
Protein-containing complex
Molecular Function
Arginine binding
Molecular Function
Arginine deiminase activity
Molecular Function
Calcium ion binding
Molecular Function
Protein homodimerization activity
Molecular Function
Protein-arginine deiminase activity
Biological Process
Arginine deiminase pathway
Biological Process
Chromatin organization
Biological Process
Chromatin remodeling
Biological Process
Histone citrullination
Biological Process
Histone H3-R26 citrullination
Biological Process
Innate immune response
Biological Process
Nucleosome assembly
Biological Process
Protein citrullination
Biological Process
Stem cell population maintenance

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Mum-2909Mus musculus89.190.01233
LLPS-Otg-3873Otolemur garnettii76.610.01047
LLPS-Ict-3934Ictidomys tridecemlineatus75.980.0 968
LLPS-Dio-1228Dipodomys ordii75.260.01012
LLPS-Eqc-4645Equus caballus74.960.01041
LLPS-Pap-0903Pan paniscus74.360.01013
LLPS-Gog-1287Gorilla gorilla74.360.01013
LLPS-Cas-0062Carlito syrichta74.320.01021
LLPS-Hos-1506Homo sapiens74.210.01011
LLPS-Pat-1053Pan troglodytes74.210.01011
LLPS-Nol-3314Nomascus leucogenys74.210.01014
LLPS-Caj-0508Callithrix jacchus74.060.01017
LLPS-Urm-1060Ursus maritimus74.020.01027
LLPS-Rhb-3597Rhinopithecus bieti73.610.01021
LLPS-Mam-3543Macaca mulatta72.860.0 999
LLPS-Cap-3217Cavia porcellus72.840.01012
LLPS-Maf-1420Macaca fascicularis72.710.0 994
LLPS-Cea-2867Cercocebus atys72.710.0 993
LLPS-Aim-1614Ailuropoda melanoleuca72.680.0 944
LLPS-Mup-2247Mustela putorius furo72.670.01003
LLPS-Chs-0642Chlorocebus sabaeus72.410.0 993
LLPS-Fec-2094Felis catus72.070.0 983
LLPS-Mal-1395Mandrillus leucophaeus71.770.0 978
LLPS-Loa-0636Loxodonta africana71.640.0 989
LLPS-Myl-0901Myotis lucifugus71.60.0 976
LLPS-Sus-0318Sus scrofa71.530.0 773
LLPS-Caf-1600Canis familiaris71.220.0 782
LLPS-Man-2522Macaca nemestrina71.170.0 973
LLPS-Aon-0488Aotus nancymaae71.130.0 962
LLPS-Bot-3135Bos taurus71.060.0 971
LLPS-Paa-3240Papio anubis70.310.0 950
LLPS-Fud-4370Fukomys damarensis68.520.0 895
LLPS-Orc-1756Oryctolagus cuniculus67.90.0 891
LLPS-Poa-1189Pongo abelii66.570.0 875
LLPS-Mod-1392Monodelphis domestica64.670.0 899
LLPS-Sah-2965Sarcophilus harrisii62.480.0 869
LLPS-Anp-3335Anas platyrhynchos55.360.0 758
LLPS-Gaga-2374Gallus gallus55.060.0 747
LLPS-Ved-1431Verticillium dahliae28.982e-40 160
LLPS-Fus-1231Fusarium solani28.882e-45 175
LLPS-Beb-1191Beauveria bassiana27.272e-41 163