LLPS-Ran-0091
Vps4a
▼ OVERVIEW
| Status: | Unreviewed |
| Protein Name: | Vacuolar protein sorting-associated protein 4A |
| Gene Name: | Vps4a, vps4-A |
| Ensembl Gene: | ENSRNOG00000020351.8 |
| Ensembl Protein: | ENSRNOP00000046690.4 |
| Organism: | Rattus norvegicus |
| Taxa ID: | 10116 |
| LLPS Type: | LLPS regulator |
▼ Classification
Condensates:
| Condensate | Evidence | Orthologs |
|---|---|---|
| Nucleolus, P-body, Postsynaptic density | Predicted from orthologs | (View) |
▼ FUNCTION
| Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis. Involved in cytokinesis: retained at the midbody by ZFYVE19/ANCHR and CHMP4C until abscission checkpoint signaling is terminated at late cytokinesis. It is then released following dephosphorylation of CHMP4C, leading to abscission. VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (By similarity). |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | ENSRNOT00000050612.5 | ENSRNOP00000046690.4 |
| UniProt | Q793F9, VPS4A_RAT | |
| GeneBank | AB076398 | BAC00961.1 |
| RefSeq | NM_145678.1 | NP_663711.1 |
| Entrez | 246772 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MAAERQPRDS GWAALGRAPR SPSDSAPTAS GSPARAESRP ARLLSSGPTT SAPPGRTEAA 60 61 SSTAGCGADP GYEMTTSTLQ KAIDLVTKAT EEDKAKNYEE ALRLYQHAVE YFLHAIKYEA 120 121 HSDKAKESIR AKCMQYLDRA EKLKDYLRNK EKHGKKPVKE NQSEGKGSDS DSEGDNPEKK 180 181 KLQEQLMGAV VMEKPNIRWN DVAGLEGAKE ALKEAVILPI KFPHLFTGKR TPWRGILLFG 240 241 PPGTGKSYLA KAVATEANNS TFFSVSSSDL MSKWLGESEK LVKNLFELAR QHKPSIIFID 300 301 EVDSLCGSRN ENESEAARRI KTEFLVQMQG VGNNNDGTLV LGATNIPWVL DSAIRRRFEK 360 361 RIYIPLPEEA ARAQMFRLHL GSTPHNLTDA NIHELARKTE GYSGADISII VRDSLMQPVR 420 421 KVQSATHFKK VCGPSRTNPS VMIDDLLTPC SPGDPGAIEM TWMDVPGDKL LEPVVCMSDM 480 481 LRSLATTRPT VNADDLLKVK KFSEDFGQES 510 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGGCCGCAG AGCGGCAGCC GCGTGACTCG GGCTGGGCCG CTCTGGGCCG CGCTCCTCGT 60 61 TCACCGTCGG ACTCGGCTCC GACTGCTAGT GGCAGCCCTG CCCGCGCCGA GAGTCGACCT 120 121 GCCAGGCTCC TGAGCAGCGG ACCTACCACC TCGGCTCCTC CGGGCCGGAC CGAGGCCGCA 180 181 AGCAGCACCG CGGGGTGTGG GGCGGACCCA GGATATGAAA TGACAACGTC AACCCTCCAG 240 241 AAAGCCATCG ATCTGGTGAC AAAAGCCACA GAAGAGGACA AAGCCAAGAA CTACGAGGAG 300 301 GCACTTCGGC TCTACCAGCA TGCTGTGGAG TATTTCCTCC ACGCCATCAA ATATGAAGCC 360 361 CACAGTGACA AAGCCAAGGA GAGCATTCGA GCAAAGTGCA TGCAGTACCT AGACAGAGCG 420 421 GAGAAGCTGA AGGATTATTT AAGAAACAAA GAGAAACATG GCAAAAAGCC AGTCAAAGAG 480 481 AATCAGAGTG AAGGCAAGGG TAGTGACAGT GACAGCGAAG GGGATAATCC AGAGAAAAAG 540 541 AAATTACAAG AACAGTTGAT GGGTGCTGTT GTGATGGAGA AGCCCAACAT TCGGTGGAAT 600 601 GATGTAGCTG GACTGGAGGG AGCTAAAGAA GCTCTCAAAG AAGCTGTGAT TTTGCCGATA 660 661 AAATTTCCAC ACTTATTCAC AGGCAAACGT ACTCCTTGGC GAGGCATACT GCTCTTTGGG 720 721 CCCCCAGGCA CAGGCAAGTC CTACCTGGCC AAAGCCGTGG CAACAGAAGC CAACAACTCT 780 781 ACCTTCTTCT CTGTGTCCTC CTCGGATCTG ATGTCTAAGT GGTTGGGGGA AAGCGAGAAG 840 841 CTAGTCAAAA ACCTGTTTGA GTTGGCCAGA CAGCACAAGC CTTCCATCAT CTTCATTGAT 900 901 GAGGTGGATT CCCTCTGCGG GTCACGGAAT GAAAATGAGA GCGAGGCAGC CCGAAGGATC 960 961 AAAACAGAGT TCTTGGTCCA AATGCAGGGA GTGGGGAATA ATAATGATGG GACTCTTGTT 1020 1021 CTTGGTGCCA CAAACATCCC CTGGGTGTTG GATTCAGCCA TCAGGAGGAG GTTTGAAAAG 1080 1081 CGAATTTATA TCCCATTGCC AGAGGAAGCG GCCCGTGCCC AGATGTTTCG GTTACACCTG 1140 1141 GGAAGCACAC CTCACAACCT CACAGATGCT AACATCCATG AGCTGGCCCG CAAGACAGAA 1200 1201 GGCTACTCAG GAGCAGATAT CAGCATTATC GTGCGGGACT CTCTCATGCA GCCAGTCAGA 1260 1261 AAAGTACAGT CAGCAACACA CTTCAAGAAG GTTTGTGGTC CTTCTCGAAC CAACCCTAGT 1320 1321 GTTATGATTG ATGATCTTCT GACCCCATGT TCTCCAGGAG ATCCAGGGGC TATAGAGATG 1380 1381 ACTTGGATGG ATGTCCCTGG TGATAAACTC TTAGAGCCTG TGGTTTGCAT GTCGGACATG 1440 1441 CTCCGGTCTC TGGCCACTAC CCGGCCCACA GTGAATGCAG ATGACCTCCT GAAAGTAAAG 1500 1501 AAATTCTCTG AGGACTTTGG ACAGGAGAGT TAA 1533 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Early endosome | |
Cellular Component | Late endosome membrane | |
Cellular Component | Midbody | |
Cellular Component | Nucleus | |
Molecular Function | ATP binding | |
Molecular Function | ATPase activity | |
Molecular Function | Rho GTPase binding | |
Biological Process | Abscission | |
Biological Process | Cell division | |
Biological Process | Endosomal transport | |
Biological Process | Mitotic cytokinesis checkpoint | |
Biological Process | Negative regulation of cytokinesis | |
Biological Process | Protein transport | |
Biological Process | Vacuole organization |
▼ ANNOTATION
| Physicochemical | ||||
| Compute pI/Mw | AAindex | |||
| Function | ||||
| PIRSF | ||||
| Localization | ||||
| COMPARTMENTS | ||||
| Expression | ||||
| ArrayExpress | TISSUES | |||
| Element | ||||
| miRWalk | RAID | microRNA.org | ||
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Mum-1587 | Mus musculus | 100.0 | 0.0 | 880 |
| LLPS-Mup-2478 | Mustela putorius furo | 99.31 | 0.0 | 878 |
| LLPS-Chs-2127 | Chlorocebus sabaeus | 99.31 | 0.0 | 875 |
| LLPS-Fud-0688 | Fukomys damarensis | 99.31 | 0.0 | 876 |
| LLPS-Caf-0571 | Canis familiaris | 99.31 | 0.0 | 874 |
| LLPS-Cas-0902 | Carlito syrichta | 99.31 | 0.0 | 878 |
| LLPS-Caj-0879 | Callithrix jacchus | 99.08 | 0.0 | 874 |
| LLPS-Fec-0830 | Felis catus | 99.08 | 0.0 | 875 |
| LLPS-Hos-1146 | Homo sapiens | 99.08 | 0.0 | 873 |
| LLPS-Mam-3655 | Macaca mulatta | 99.07 | 0.0 | 863 |
| LLPS-Bot-0837 | Bos taurus | 99.01 | 0.0 | 809 |
| LLPS-Cap-2750 | Cavia porcellus | 98.86 | 0.0 | 872 |
| LLPS-Poa-0095 | Pongo abelii | 98.86 | 0.0 | 872 |
| LLPS-Ict-3080 | Ictidomys tridecemlineatus | 98.63 | 0.0 | 868 |
| LLPS-Otg-0663 | Otolemur garnettii | 98.38 | 0.0 | 855 |
| LLPS-Mea-3071 | Mesocricetus auratus | 98.18 | 0.0 | 861 |
| LLPS-Urm-0919 | Ursus maritimus | 98.18 | 0.0 | 867 |
| LLPS-Sus-0550 | Sus scrofa | 98.17 | 0.0 | 868 |
| LLPS-Loa-3531 | Loxodonta africana | 98.15 | 0.0 | 853 |
| LLPS-Aim-2510 | Ailuropoda melanoleuca | 97.95 | 0.0 | 868 |
| LLPS-Eqc-1220 | Equus caballus | 97.77 | 0.0 | 801 |
| LLPS-Pap-1307 | Pan paniscus | 97.72 | 0.0 | 859 |
| LLPS-Orc-0586 | Oryctolagus cuniculus | 97.59 | 0.0 | 876 |
| LLPS-Tut-1477 | Tursiops truncatus | 97.47 | 0.0 | 856 |
| LLPS-Cea-0130 | Cercocebus atys | 94.89 | 0.0 | 931 |
| LLPS-Paa-0674 | Papio anubis | 94.7 | 0.0 | 931 |
| LLPS-Mod-1502 | Monodelphis domestica | 94.44 | 0.0 | 826 |
| LLPS-Sah-1239 | Sarcophilus harrisii | 94.43 | 0.0 | 824 |
| LLPS-Nol-0499 | Nomascus leucogenys | 94.31 | 0.0 | 929 |
| LLPS-Maf-1127 | Macaca fascicularis | 94.3 | 0.0 | 910 |
| LLPS-Man-1490 | Macaca nemestrina | 94.3 | 0.0 | 910 |
| LLPS-Pat-3409 | Pan troglodytes | 94.14 | 0.0 | 927 |
| LLPS-Dio-0319 | Dipodomys ordii | 94.05 | 0.0 | 831 |
| LLPS-Aon-3738 | Aotus nancymaae | 93.41 | 0.0 | 917 |
| LLPS-Ora-0436 | Ornithorhynchus anatinus | 93.27 | 0.0 | 820 |
| LLPS-Anc-0212 | Anolis carolinensis | 93.12 | 0.0 | 828 |
| LLPS-Myl-2869 | Myotis lucifugus | 93.0 | 0.0 | 843 |
| LLPS-Mal-1726 | Mandrillus leucophaeus | 92.37 | 0.0 | 863 |
| LLPS-Leo-1432 | Lepisosteus oculatus | 91.3 | 0.0 | 820 |
| LLPS-Scf-0592 | Scleropages formosus | 91.28 | 0.0 | 820 |
| LLPS-Xim-0156 | Xiphophorus maculatus | 90.84 | 0.0 | 763 |
| LLPS-Gaga-1094 | Gallus gallus | 90.83 | 0.0 | 818 |
| LLPS-Xet-0204 | Xenopus tropicalis | 90.37 | 0.0 | 820 |
| LLPS-Orn-1736 | Oreochromis niloticus | 90.37 | 0.0 | 821 |
| LLPS-Pof-1682 | Poecilia formosa | 89.91 | 0.0 | 800 |
| LLPS-Meg-0044 | Meleagris gallopavo | 89.75 | 0.0 | 738 |
| LLPS-Lac-0057 | Latimeria chalumnae | 89.33 | 0.0 | 802 |
| LLPS-Dar-0141 | Danio rerio | 89.11 | 0.0 | 750 |
| LLPS-Ten-3539 | Tetraodon nigroviridis | 89.1 | 0.0 | 795 |
| LLPS-Tar-0557 | Takifugu rubripes | 89.04 | 0.0 | 796 |
| LLPS-Scm-1823 | Scophthalmus maximus | 88.53 | 0.0 | 808 |
| LLPS-Orl-3324 | Oryzias latipes | 88.3 | 0.0 | 803 |
| LLPS-Asm-0401 | Astyanax mexicanus | 88.17 | 0.0 | 793 |
| LLPS-Gaa-0811 | Gasterosteus aculeatus | 87.61 | 0.0 | 802 |
| LLPS-Icp-1474 | Ictalurus punctatus | 81.34 | 0.0 | 701 |
| LLPS-Gog-3079 | Gorilla gorilla | 80.68 | 0.0 | 734 |
| LLPS-Rhb-3669 | Rhinopithecus bieti | 80.45 | 0.0 | 734 |
| LLPS-Ova-1169 | Ovis aries | 80.23 | 0.0 | 735 |
| LLPS-Ere-0016 | Erinaceus europaeus | 80.1 | 0.0 | 659 |
| LLPS-Anp-3138 | Anas platyrhynchos | 79.72 | 0.0 | 690 |
| LLPS-Fia-0651 | Ficedula albicollis | 79.54 | 0.0 | 714 |
| LLPS-Pes-0723 | Pelodiscus sinensis | 79.08 | 0.0 | 689 |
| LLPS-Tag-2911 | Taeniopygia guttata | 78.57 | 0.0 | 706 |
| LLPS-Put-0907 | Puccinia triticina | 77.99 | 9e-83 | 264 |
| LLPS-Drm-1454 | Drosophila melanogaster | 73.23 | 0.0 | 649 |
| LLPS-Cis-1612 | Ciona savignyi | 70.72 | 5e-112 | 338 |
| LLPS-Cii-1044 | Ciona intestinalis | 67.97 | 0.0 | 606 |
| LLPS-Cae-0121 | Caenorhabditis elegans | 67.43 | 0.0 | 602 |
| LLPS-Crn-0895 | Cryptococcus neoformans | 59.77 | 6e-179 | 518 |
| LLPS-Tum-1042 | Tuber melanosporum | 59.36 | 3e-178 | 515 |
| LLPS-Mel-0130 | Melampsora laricipopulina | 58.96 | 2e-174 | 506 |
| LLPS-Hov-0585 | Hordeum vulgare | 58.75 | 3e-136 | 411 |
| LLPS-Asg-0429 | Ashbya gossypii | 58.72 | 3e-175 | 508 |
| LLPS-Pug-0580 | Puccinia graminis | 58.62 | 5e-176 | 510 |
| LLPS-Sac-1212 | Saccharomyces cerevisiae | 58.28 | 1e-176 | 511 |
| LLPS-Asni-0022 | Aspergillus niger | 58.24 | 2e-172 | 501 |
| LLPS-Scc-1154 | Schizosaccharomyces cryophilus | 58.16 | 1e-166 | 486 |
| LLPS-Asf-0380 | Aspergillus flavus | 57.95 | 7e-172 | 499 |
| LLPS-Asfu-0833 | Aspergillus fumigatus | 57.85 | 2e-171 | 498 |
| LLPS-Kop-0338 | Komagataella pastoris | 57.83 | 2e-173 | 503 |
| LLPS-Asn-0968 | Aspergillus nidulans | 57.82 | 5e-176 | 510 |
| LLPS-Scp-1222 | Schizosaccharomyces pombe | 57.74 | 9e-166 | 484 |
| LLPS-Nec-1292 | Neurospora crassa | 57.72 | 9e-175 | 507 |
| LLPS-Miv-1422 | Microbotryum violaceum | 57.63 | 1e-173 | 504 |
| LLPS-Spr-0962 | Sporisorium reilianum | 57.45 | 1e-178 | 518 |
| LLPS-Nef-0231 | Neosartorya fischeri | 57.4 | 1e-170 | 496 |
| LLPS-Asc-0067 | Aspergillus clavatus | 57.34 | 5e-173 | 502 |
| LLPS-Gas-0335 | Galdieria sulphuraria | 57.21 | 3e-170 | 496 |
| LLPS-Chr-1513 | Chlamydomonas reinhardtii | 57.21 | 7e-167 | 486 |
| LLPS-Lem-0542 | Leptosphaeria maculans | 57.05 | 8e-168 | 489 |
| LLPS-Usm-0594 | Ustilago maydis | 57.02 | 6e-178 | 516 |
| LLPS-Yal-0634 | Yarrowia lipolytica | 56.98 | 2e-170 | 495 |
| LLPS-Dos-0949 | Dothistroma septosporum | 56.92 | 1e-170 | 496 |
| LLPS-Cym-0316 | Cyanidioschyzon merolae | 56.87 | 2e-127 | 389 |
| LLPS-Blg-0019 | Blumeria graminis | 56.86 | 7e-174 | 504 |
| LLPS-Pytr-0885 | Pyrenophora triticirepentis | 56.49 | 6e-162 | 474 |
| LLPS-Ast-0832 | Aspergillus terreus | 56.36 | 6e-172 | 499 |
| LLPS-Aso-1228 | Aspergillus oryzae | 56.04 | 3e-168 | 491 |
| LLPS-Pyt-0568 | Pyrenophora teres | 55.81 | 1e-157 | 463 |
| LLPS-Zyt-0226 | Zymoseptoria tritici | 55.78 | 1e-167 | 488 |
| LLPS-Gag-0359 | Gaeumannomyces graminis | 55.26 | 6e-158 | 465 |
| LLPS-Mao-0131 | Magnaporthe oryzae | 55.14 | 1e-160 | 471 |
| LLPS-Scj-1478 | Schizosaccharomyces japonicus | 54.44 | 7e-161 | 471 |
| LLPS-Map-0592 | Magnaporthe poae | 54.27 | 4e-163 | 478 |
| LLPS-Ved-0260 | Verticillium dahliae | 54.04 | 5e-159 | 466 |
| LLPS-Cog-0741 | Colletotrichum gloeosporioides | 53.88 | 1e-157 | 462 |
| LLPS-Orr-2069 | Oryza rufipogon | 53.78 | 4e-146 | 446 |
| LLPS-Orb-0309 | Oryza barthii | 53.53 | 2e-146 | 446 |
| LLPS-Mua-2472 | Musa acuminata | 53.51 | 2e-156 | 459 |
| LLPS-Bro-0918 | Brassica oleracea | 53.51 | 7e-154 | 453 |
| LLPS-Pot-0885 | Populus trichocarpa | 53.36 | 3e-155 | 457 |
| LLPS-Brr-2381 | Brassica rapa | 53.26 | 2e-152 | 449 |
| LLPS-Brn-1915 | Brassica napus | 53.24 | 2e-153 | 452 |
| LLPS-Glm-2644 | Glycine max | 53.14 | 3e-151 | 447 |
| LLPS-Osl-0483 | Ostreococcus lucimarinus | 53.14 | 6e-150 | 444 |
| LLPS-Beb-1235 | Beauveria bassiana | 53.08 | 1e-154 | 455 |
| LLPS-Fus-0219 | Fusarium solani | 53.08 | 1e-154 | 455 |
| LLPS-Cogr-1560 | Colletotrichum graminicola | 53.05 | 3e-155 | 457 |
| LLPS-Orgl-1181 | Oryza glumaepatula | 53.03 | 4e-147 | 447 |
| LLPS-Ori-0496 | Oryza indica | 53.03 | 6e-152 | 448 |
| LLPS-Orni-0044 | Oryza nivara | 53.03 | 5e-146 | 447 |
| LLPS-Coo-1010 | Colletotrichum orbiculare | 53.03 | 4e-156 | 459 |
| LLPS-Org-1671 | Oryza glaberrima | 53.03 | 6e-152 | 448 |
| LLPS-Orp-1303 | Oryza punctata | 53.03 | 6e-152 | 448 |
| LLPS-Orbr-1690 | Oryza brachyantha | 53.03 | 1e-151 | 447 |
| LLPS-Trr-0807 | Trichoderma reesei | 52.95 | 6e-155 | 456 |
| LLPS-Art-2900 | Arabidopsis thaliana | 52.89 | 8e-153 | 451 |
| LLPS-Thc-2121 | Theobroma cacao | 52.88 | 1e-152 | 450 |
| LLPS-Via-0535 | Vigna angularis | 52.81 | 3e-151 | 447 |
| LLPS-Vir-0212 | Vigna radiata | 52.81 | 2e-151 | 447 |
| LLPS-Coc-0217 | Corchorus capsularis | 52.78 | 1e-151 | 448 |
| LLPS-Php-1576 | Physcomitrella patens | 52.77 | 4e-154 | 454 |
| LLPS-Nia-2320 | Nicotiana attenuata | 52.69 | 3e-153 | 452 |
| LLPS-Phv-2579 | Phaseolus vulgaris | 52.69 | 1e-148 | 440 |
| LLPS-Arl-2110 | Arabidopsis lyrata | 52.67 | 2e-152 | 450 |
| LLPS-Gor-0325 | Gossypium raimondii | 52.65 | 8e-153 | 451 |
| LLPS-Chc-0879 | Chondrus crispus | 52.64 | 7e-151 | 446 |
| LLPS-Fuv-1141 | Fusarium verticillioides | 52.62 | 5e-154 | 454 |
| LLPS-Fuo-0279 | Fusarium oxysporum | 52.62 | 2e-154 | 454 |
| LLPS-Viv-0676 | Vitis vinifera | 52.58 | 2e-155 | 457 |
| LLPS-Sot-1975 | Solanum tuberosum | 52.48 | 2e-151 | 447 |
| LLPS-Hea-2551 | Helianthus annuus | 52.47 | 1e-149 | 442 |
| LLPS-Amt-0912 | Amborella trichopoda | 52.46 | 1e-153 | 453 |
| LLPS-Cus-1022 | Cucumis sativus | 52.36 | 3e-152 | 449 |
| LLPS-Zem-2303 | Zea mays | 52.35 | 3e-152 | 449 |
| LLPS-Trv-0993 | Trichoderma virens | 52.27 | 3e-153 | 452 |
| LLPS-Mae-1865 | Manihot esculenta | 52.24 | 2e-154 | 454 |
| LLPS-Sei-2178 | Setaria italica | 52.22 | 4e-152 | 449 |
| LLPS-Prp-0208 | Prunus persica | 52.13 | 1e-153 | 453 |
| LLPS-Sol-2004 | Solanum lycopersicum | 52.11 | 8e-149 | 441 |
| LLPS-Dac-0537 | Daucus carota | 51.99 | 9e-149 | 441 |
| LLPS-Sob-1314 | Sorghum bicolor | 51.79 | 3e-152 | 449 |
| LLPS-Tra-0488 | Triticum aestivum | 51.78 | 3e-153 | 452 |
| LLPS-Met-0938 | Medicago truncatula | 51.78 | 3e-150 | 444 |
| LLPS-Brd-1758 | Brachypodium distachyon | 51.56 | 7e-152 | 448 |
| LLPS-Sem-1833 | Selaginella moellendorffii | 51.45 | 2e-150 | 445 |
| LLPS-Lep-2095 | Leersia perrieri | 51.23 | 4e-150 | 444 |
| LLPS-Orm-0701 | Oryza meridionalis | 51.15 | 1e-143 | 427 |
| LLPS-Ors-0361 | Oryza sativa | 51.02 | 3e-144 | 429 |
| LLPS-Phn-0529 | Phaeosphaeria nodorum | 50.91 | 5e-137 | 410 |
| LLPS-Tru-1688 | Triticum urartu | 45.4 | 2e-80 | 267 |