LLPS-Ran-0005
Eif4a2

▼ OVERVIEW

Status: Reviewed
Protein Name: Eukaryotic initiation factor 4A-II
Gene Name: Eif4a2
Ensembl Gene: ENSRNOG00000001815.6
Ensembl Protein: ENSRNOP00000002484.5
Organism: Rattus norvegicus
Taxa ID: 10116
LLPS Type: LLPS regulator


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Stress granule
"...We analyzed SGs by immunofluorescence colocalization of granule-associated protein T-cell internal antigen-1 with eIF3b, eIF4E, and ribosomal protein S6 and studied eIF2 and eIF4F complex. The results showed that IR stress induced SG formation in the CA1 region after 3-day reperfusion, consistent with TI and DND in CA1. SGs were formed independently of eIF2α phosphorylation, and their appearance was correlated with a decrease in the levels of eIF4F compounds, the cap-binding protein eIF4E, and eIF4B, suggesting that remodeling of the eIF4F complex was required for SG formation."
Adult male Wistar rats27836976
Nucleolus, Postsynaptic densityPredicted from orthologsN/A(View)

▼ FUNCTION

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon (By similarity).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MSGGSADYNR  EHGGPEGMDP  DGVIESNWNE  IVDNFDDMNL  KESLLRGIYA  YGFEKPSAIQ  60
61    QRAIIPCIKG  YDVIAQAQSG  TGKTATFAIS  ILQQLEIEFK  ETQALVLAPT  RELAQQIQKV  120
121   ILALGDYMGA  TCHACIGGTN  VRNEMQKLQA  EAPHIVVGTP  GRVFDMLNRR  YLSPKWIKMF  180
181   VLDEADEMLS  RGFKDQIYEI  FQKLNTSIQV  VLLSATMPTD  VLEVTKKFMR  DPIRILVKKE  240
241   ELTLEGIKQF  YINVEREEWK  LDTLCDLYET  LTITQAVIFL  NTRRKVDWLT  EKMHARDFTV  300
301   SALHGDMDQK  ERDVIMREFR  SGSSRVLITT  DLLARGIDVQ  QVSLVINYDL  PTNRENYIHR  360
361   IGRGGRFGRK  GVAINFVTEE  DKRILRDIET  FYNTTVEEMP  MNVADLI  407
Nucleotide CDS Sequence (FASTA)
1     ATGTCTGGTG  GCTCCGCGGA  TTACAACAGA  GAACATGGCG  GCCCAGAGGG  AATGGACCCC  60
61    GATGGTGTCA  TCGAGAGCAA  CTGGAATGAA  ATTGTTGATA  ACTTTGATGA  TATGAATTTA  120
121   AAGGAGTCCC  TTCTTCGAGG  CATCTATGCT  TATGGTTTTG  AGAAGCCTTC  AGCTATTCAG  180
181   CAGAGAGCTA  TAATCCCATG  TATTAAAGGA  TATGATGTGA  TTGCTCAAGC  TCAGTCAGGT  240
241   ACTGGCAAGA  CAGCCACATT  TGCTATTTCC  ATCCTGCAAC  AGTTGGAGAT  TGAGTTCAAG  300
301   GAGACCCAAG  CACTAGTATT  GGCCCCCACC  AGAGAACTGG  CTCAACAGAT  CCAAAAGGTC  360
361   ATTTTGGCTC  TTGGAGATTA  TATGGGAGCA  ACTTGTCATG  CTTGCATTGG  AGGAACAAAT  420
421   GTTCGAAATG  AAATGCAGAA  GTTGCAGGCT  GAAGCCCCTC  ATATTGTTGT  TGGCACTCCT  480
481   GGGAGAGTAT  TTGATATGCT  AAACAGAAGA  TACCTTTCTC  CAAAATGGAT  CAAAATGTTT  540
541   GTTTTGGATG  AAGCAGATGA  AATGTTGAGC  CGAGGGTTTA  AGGATCAGAT  CTATGAGATT  600
601   TTCCAAAAAT  TAAATACAAG  CATTCAGGTT  GTGTTACTTT  CTGCCACAAT  GCCAACTGAT  660
661   GTCCTAGAAG  TGACCAAGAA  ATTCATGAGA  GATCCAATTC  GAATTCTGGT  GAAGAAGGAA  720
721   GAATTGACCC  TTGAAGGAAT  TAAACAATTT  TATATTAATG  TTGAGCGAGA  GGAGTGGAAG  780
781   CTGGACACTC  TTTGTGACTT  GTATGAGACT  TTGACTATCA  CACAAGCAGT  TATTTTTCTC  840
841   AATACAAGGC  GCAAGGTGGA  CTGGCTCACG  GAGAAAATGC  ATGCCAGGGA  CTTCACAGTT  900
901   TCTGCTCTGC  ATGGTGACAT  GGACCAGAAG  GAAAGAGATG  TCATCATGAG  GGAATTCCGA  960
961   TCAGGGTCAA  GCCGTGTTCT  GATCACTACT  GACTTGTTGG  CTCGTGGGAT  TGATGTGCAA  1020
1021  CAAGTGTCCT  TGGTTATAAA  CTATGATCTA  CCTACCAATC  GTGAAAACTA  TATTCACAGA  1080
1081  ATTGGCAGAG  GGGGTCGATT  TGGGAGGAAA  GGTGTGGCTA  TAAACTTTGT  TACTGAAGAA  1140
1141  GACAAGAGGA  TTCTTCGTGA  CATTGAGACT  TTCTACAATA  CTACAGTGGA  GGAAATGCCC  1200
1201  ATGAATGTGG  CTGACCTAAT  TTAA  1224

▼ KEYWORD

ID
Family
ATP-binding
Complete proteome
Direct protein sequencing
Helicase
Hydrolase
Initiation factor
Nucleotide-binding
Phosphoprotein
Protein biosynthesis
Reference proteome
RNA-binding

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Perinuclear region of cytoplasm
Molecular Function
ATP binding
Molecular Function
ATP-dependent RNA helicase activity
Molecular Function
Translation initiation factor activity
Biological Process
Cellular response to leukemia inhibitory factor
Biological Process
Negative regulation of RNA-directed 5'-3' RNA polymerase activity

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Chs-0017Chlorocebus sabaeus100.00.0 748
LLPS-Cea-2370Cercocebus atys100.00.0 815
LLPS-Sus-0086Sus scrofa100.00.0 815
LLPS-Gog-0745Gorilla gorilla100.00.0 815
LLPS-Fud-0629Fukomys damarensis100.00.0 815
LLPS-Dio-3485Dipodomys ordii100.00.0 748
LLPS-Bot-1221Bos taurus100.00.0 815
LLPS-Mal-2259Mandrillus leucophaeus100.00.0 815
LLPS-Mup-0432Mustela putorius furo100.00.0 748
LLPS-Pap-0552Pan paniscus100.00.0 815
LLPS-Urm-1221Ursus maritimus100.00.0 815
LLPS-Fec-1541Felis catus100.00.0 815
LLPS-Loa-2282Loxodonta africana100.00.0 795
LLPS-Otg-1452Otolemur garnettii100.00.0 815
LLPS-Orc-2473Oryctolagus cuniculus100.00.0 815
LLPS-Poa-0769Pongo abelii100.00.0 815
LLPS-Caf-2831Canis familiaris100.00.0 815
LLPS-Eqc-0161Equus caballus100.00.0 795
LLPS-Cap-1449Cavia porcellus100.00.0 815
LLPS-Cas-0113Carlito syrichta100.00.0 815
LLPS-Maf-2353Macaca fascicularis99.750.0 811
LLPS-Mum-4271Mus musculus99.750.0 811
LLPS-Aon-0787Aotus nancymaae99.750.0 811
LLPS-Caj-2333Callithrix jacchus99.750.0 811
LLPS-Paa-1394Papio anubis99.750.0 811
LLPS-Aim-0711Ailuropoda melanoleuca99.750.0 811
LLPS-Pat-2795Pan troglodytes99.750.0 811
LLPS-Hos-0639Homo sapiens99.750.0 811
LLPS-Man-0241Macaca nemestrina99.750.0 811
LLPS-Ict-0382Ictidomys tridecemlineatus99.750.0 795
LLPS-Rhb-0024Rhinopithecus bieti99.510.0 803
LLPS-Nol-1872Nomascus leucogenys99.510.0 808
LLPS-Mea-1224Mesocricetus auratus99.50.0 801
LLPS-Myl-1979Myotis lucifugus99.260.0 806
LLPS-Mam-1480Macaca mulatta98.530.0 798
LLPS-Xet-0198Xenopus tropicalis98.280.0 803
LLPS-Gaga-1395Gallus gallus98.210.0 763
LLPS-Anp-1216Anas platyrhynchos98.030.0 801
LLPS-Anc-1175Anolis carolinensis98.030.0 795
LLPS-Ova-0347Ovis aries97.790.0 798
LLPS-Fia-0701Ficedula albicollis97.790.0 800
LLPS-Tag-1189Taeniopygia guttata97.790.0 800
LLPS-Pes-0010Pelodiscus sinensis97.560.0 792
LLPS-Leo-0248Lepisosteus oculatus97.080.0 795
LLPS-Dar-1932Danio rerio95.620.0 787
LLPS-Xim-0442Xiphophorus maculatus95.130.0 782
LLPS-Scf-0893Scleropages formosus95.130.0 781
LLPS-Orl-1039Oryzias latipes94.890.0 780
LLPS-Asm-0673Astyanax mexicanus94.650.0 781
LLPS-Icp-0210Ictalurus punctatus94.650.0 779
LLPS-Mod-0511Monodelphis domestica94.590.0 753
LLPS-Pof-0430Poecilia formosa94.440.0 776
LLPS-Orn-0422Oreochromis niloticus94.40.0 777
LLPS-Lac-1643Latimeria chalumnae93.640.0 755
LLPS-Scm-0767Scophthalmus maximus93.220.0 734
LLPS-Meg-2838Meleagris gallopavo92.980.0 727
LLPS-Gaa-1998Gasterosteus aculeatus92.910.0 759
LLPS-Tar-1057Takifugu rubripes92.670.0 757
LLPS-Sah-0483Sarcophilus harrisii89.930.0 739
LLPS-Tut-1572Tursiops truncatus89.880.0 742
LLPS-Ere-0487Erinaceus europaeus89.880.0 742
LLPS-Ora-3138Ornithorhynchus anatinus80.810.0 615
LLPS-Cis-1713Ciona savignyi80.210.0 632
LLPS-Cii-0503Ciona intestinalis80.10.0 640
LLPS-Abg-1766Absidia glauca79.170.0 610
LLPS-Gag-0704Gaeumannomyces graminis78.350.0 609
LLPS-Map-0309Magnaporthe poae78.350.0 609
LLPS-Coo-0455Colletotrichum orbiculare78.120.0 602
LLPS-Fuv-1217Fusarium verticillioides78.090.0 609
LLPS-Blg-0118Blumeria graminis78.090.0 610
LLPS-Nef-0114Neosartorya fischeri78.090.0 599
LLPS-Fus-1205Fusarium solani78.090.0 609
LLPS-Fuo-0017Fusarium oxysporum78.090.0 609
LLPS-Cogr-1035Colletotrichum graminicola78.090.0 610
LLPS-Mao-1548Magnaporthe oryzae77.840.0 608
LLPS-Beb-0407Beauveria bassiana77.840.0 607
LLPS-Trr-0339Trichoderma reesei77.840.0 610
LLPS-Trv-1482Trichoderma virens77.840.0 609
LLPS-Scs-0229Sclerotinia sclerotiorum77.780.0 603
LLPS-Miv-1473Microbotryum violaceum77.690.0 599
LLPS-Asfu-0400Aspergillus fumigatus77.660.0 592
LLPS-Pyt-0800Pyrenophora teres77.580.0 602
LLPS-Nec-1472Neurospora crassa77.580.0 605
LLPS-Cog-0451Colletotrichum gloeosporioides77.580.0 607
LLPS-Pytr-0699Pyrenophora triticirepentis77.580.0 602
LLPS-Asc-1469Aspergillus clavatus77.320.0 599
LLPS-Phn-0194Phaeosphaeria nodorum77.320.0 602
LLPS-Usm-0562Ustilago maydis77.260.0 604
LLPS-Aso-0030Aspergillus oryzae77.20.0 589
LLPS-Ved-0239Verticillium dahliae77.00.0 599
LLPS-Asf-1130Aspergillus flavus76.880.0 586
LLPS-Ten-1272Tetraodon nigroviridis76.850.0 585
LLPS-Asn-0694Aspergillus nidulans76.80.0 595
LLPS-Spr-0452Sporisorium reilianum76.740.0 602
LLPS-Scj-1227Schizosaccharomyces japonicus76.230.0 597
LLPS-Asni-0716Aspergillus niger76.030.0 592
LLPS-Scc-1071Schizosaccharomyces cryophilus75.970.0 593
LLPS-Scp-0609Schizosaccharomyces pombe75.970.0 593
LLPS-Ast-0957Aspergillus terreus75.650.0 584
LLPS-Crn-0653Cryptococcus neoformans75.510.0 595
LLPS-Mel-0769Melampsora laricipopulina75.310.0 592
LLPS-Tum-0606Tuber melanosporum75.310.0 594
LLPS-Hov-0380Hordeum vulgare74.610.0 566
LLPS-Brd-2317Brachypodium distachyon74.610.0 565
LLPS-Orbr-0570Oryza brachyantha74.610.0 564
LLPS-Orb-0573Oryza barthii74.350.0 566
LLPS-Orni-0034Oryza nivara74.350.0 564
LLPS-Orgl-1091Oryza glumaepatula74.350.0 566
LLPS-Sei-1565Setaria italica74.350.0 564
LLPS-Ors-0142Oryza sativa74.350.0 566
LLPS-Pot-1200Populus trichocarpa74.350.0 565
LLPS-Orp-0021Oryza punctata74.350.0 566
LLPS-Orr-1618Oryza rufipogon74.350.0 566
LLPS-Org-0150Oryza glaberrima74.350.0 566
LLPS-Tra-0833Triticum aestivum74.090.0 567
LLPS-Chc-0540Chondrus crispus74.090.0 578
LLPS-Tru-1309Triticum urartu74.090.0 565
LLPS-Ori-0021Oryza indica74.080.0 563
LLPS-Thc-0080Theobroma cacao74.080.0 561
LLPS-Sot-1434Solanum tuberosum74.080.0 563
LLPS-Sob-0683Sorghum bicolor74.080.0 565
LLPS-Amt-0707Amborella trichopoda74.080.0 565
LLPS-Orm-0024Oryza meridionalis74.080.0 563
LLPS-Drm-0038Drosophila melanogaster73.860.0 599
LLPS-Sol-1120Solanum lycopersicum73.820.0 560
LLPS-Hea-0707Helianthus annuus73.820.0 560
LLPS-Php-0301Physcomitrella patens73.820.0 557
LLPS-Via-1041Vigna angularis73.820.0 556
LLPS-Met-0986Medicago truncatula73.820.0 565
LLPS-Vir-0938Vigna radiata73.820.0 556
LLPS-Viv-0946Vitis vinifera73.820.0 563
LLPS-Gor-0905Gossypium raimondii73.820.0 560
LLPS-Phv-2542Phaseolus vulgaris73.820.0 557
LLPS-Mae-0189Manihot esculenta73.820.0 562
LLPS-Prp-0320Prunus persica73.820.0 561
LLPS-Zem-0311Zea mays73.820.0 562
LLPS-Glm-2109Glycine max73.560.0 558
LLPS-Nia-2480Nicotiana attenuata73.560.0 561
LLPS-Sem-1838Selaginella moellendorffii73.40.0 563
LLPS-Mua-0617Musa acuminata73.30.0 561
LLPS-Cus-0456Cucumis sativus73.30.0 561
LLPS-Brr-1242Brassica rapa73.30.0 553
LLPS-Arl-1993Arabidopsis lyrata73.040.0 555
LLPS-Art-0263Arabidopsis thaliana73.040.0 553
LLPS-Bro-0062Brassica oleracea73.040.0 555
LLPS-Brn-0658Brassica napus72.510.0 551
LLPS-Cae-0890Caenorhabditis elegans72.050.0 562
LLPS-Put-0968Puccinia triticina71.740.0 556
LLPS-Osl-0029Ostreococcus lucimarinus69.660.0 558