• Disorder▼
  • Domain▼
  • PTM▼
  • Variation▼
  • Mutation▼
  • Interaction▼
  • Disease▼
  • Drug▼
  • Physicochemical▼
  • Function▼
  • Proteomics▼
  • Structure▼
  • Localization▼
  • Expression▼
  • Element▼
  • Methylation▼

LLPS-Ova-1652

Integrated Annotations

▼ OVERVIEW

Status: Unreviewed
Protein Name: DNA topoisomerase I; DNA topoisomerase 1
Ensembl Gene: ENSOARG00000008628.1
Ensembl Protein: ENSOARP00000009259.1
Organism: Ovis aries
Taxa ID: 9940
LLPS Type: Others


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
NucleolusPredicted from orthologs(View)

▼ FUNCTION

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

▼ CROSS REFERENCE

DatabaseNucleotide IDProtein ID
EnsemblENSOART00000009395.1ENSOARP00000009259.1
UniProtW5PFP4, W5PFP4_SHEEP
GeneBankAMGL01071526

▼ SEQUENCE

Protein Sequence (FASTA)
1     MSGDHLHNDS  QIEVDFRLND  SHKHKDKHKD  REHRHREHKK  DKEEDLEKSK  HSNSEHKDSE  60
61    KKHKEKEKTK  HKDKHKDKHK  DRDKEKRKEE  KIKASGDAKI  KKEKENGFSS  PPRIKDEPED  120
121   DGYFASPKED  IKPLKRPRDE  DDADFKPKKI  KTEDIKKEKK  RKLEEEEDGK  LKKTKNKDKD  180
181   KKVPEPDSKK  KKPKKEEEQK  WKWWEEERYP  EGIKWKFLEH  KGPVFAPPYE  PLPENVKFYY  240
241   DGKVMKLSPK  AEEVATFFAK  MLNHEYTTKE  IFRKNFFKDW  RKEMTNEEKN  IITNLSKCDF  300
301   TQMSQYFKAQ  TEARKQMSKE  EKLKIKEENE  KLLKEYGFCV  MDNHRERIAN  FKIEPPGLFR  360
361   GRGNHPKMGM  LKRRIMPEDI  IINCSKDAKV  PSPPPGHKWK  EVRHDNKVTW  LVSWTENIQG  420
421   SIKYIMLNPS  SRIKGEKDWQ  KYETARRLKK  CVDKIRNQYR  EDWKSKEMKV  RQRAVALCFI  480
481   DKLALRAGNE  KEEGETADTV  GCCSLRVEHI  NLHPELDGQE  YVVEFDFLGK  DSIRYYNKVP  540
541   VEKRVFKNLQ  LFMENKQPED  DLFDRLNTGI  LNKHLQDLME  GLTAKVFRTY  NASITLQQQL  600
601   KELTA  605
Nucleotide CDS Sequence (FASTA)
1     ATGAGTGGGG  ACCATCTCCA  CAACGATTCC  CAGATCGAGG  TGGATTTCCG  ACTGAATGAT  60
61    TCTCATAAAC  ACAAAGATAA  GCATAAAGAT  CGAGAACACC  GGCACAGAGA  GCACAAGAAG  120
121   GACAAGGAGG  AGGACCTAGA  GAAGTCCAAG  CACAGCAACA  GTGAACATAA  AGATTCTGAA  180
181   AAGAAACACA  AAGAGAAGGA  GAAAACCAAA  CACAAAGATA  AGCATAAAGA  CAAGCACAAA  240
241   GACAGAGACA  AGGAAAAACG  AAAGGAAGAA  AAGATTAAAG  CTTCTGGGGA  TGCAAAAATA  300
301   AAAAAAGAGA  AGGAAAATGG  CTTTTCGAGT  CCACCACGGA  TTAAAGATGA  ACCTGAAGAT  360
361   GATGGCTATT  TTGCTTCTCC  TAAAGAGGAT  ATAAAGCCAT  TAAAGAGACC  TCGGGATGAG  420
421   GATGATGCTG  ATTTTAAACC  TAAGAAAATT  AAAACAGAAG  ATATCAAGAA  AGAGAAGAAA  480
481   CGAAAACTAG  AGGAAGAAGA  GGATGGTAAA  TTGAAAAAAA  CCAAGAATAA  AGATAAAGAT  540
541   AAAAAAGTTC  CTGAGCCAGA  TAGCAAGAAA  AAGAAGCCGA  AGAAGGAAGA  GGAGCAGAAA  600
601   TGGAAATGGT  GGGAAGAAGA  ACGCTATCCT  GAAGGCATCA  AATGGAAATT  CCTAGAACAT  660
661   AAAGGTCCTG  TATTCGCTCC  ACCATATGAA  CCTCTTCCAG  AGAATGTCAA  GTTTTACTAT  720
721   GATGGTAAAG  TCATGAAGCT  GAGCCCCAAA  GCAGAAGAAG  TAGCTACGTT  CTTTGCAAAA  780
781   ATGCTCAACC  ATGAATATAC  TACTAAGGAA  ATATTTAGGA  AAAATTTCTT  TAAAGACTGG  840
841   AGAAAGGAAA  TGACCAATGA  AGAGAAGAAT  ATTATCACCA  ACCTCAGCAA  ATGTGATTTT  900
901   ACCCAGATGA  GCCAGTATTT  CAAAGCCCAG  ACAGAAGCTC  GGAAGCAGAT  GAGCAAGGAA  960
961   GAGAAACTGA  AAATCAAAGA  GGAGAATGAA  AAATTACTGA  AAGAATATGG  CTTTTGTGTT  1020
1021  ATGGACAACC  ACAGAGAGAG  GATCGCCAAC  TTCAAGATAG  AACCTCCTGG  GCTTTTCCGT  1080
1081  GGCCGCGGCA  ACCACCCCAA  GATGGGCATG  CTGAAGAGAC  GAATCATGCC  TGAGGATATA  1140
1141  ATCATCAACT  GTAGCAAAGA  TGCCAAGGTT  CCTTCTCCTC  CTCCAGGTCA  TAAGTGGAAA  1200
1201  GAAGTCCGAC  ACGATAACAA  GGTTACTTGG  CTAGTCTCCT  GGACAGAGAA  TATCCAAGGT  1260
1261  TCTATTAAAT  ACATCATGCT  GAACCCCAGC  TCACGAATCA  AGGGTGAGAA  AGACTGGCAG  1320
1321  AAATATGAGA  CTGCTCGGAG  GCTGAAGAAG  TGTGTGGATA  AGATCCGGAA  CCAATATCGG  1380
1381  GAAGACTGGA  AGTCCAAAGA  GATGAAAGTC  CGGCAGAGAG  CTGTAGCCCT  GTGCTTCATT  1440
1441  GATAAGCTTG  CTCTGCGAGC  AGGCAACGAG  AAGGAGGAAG  GAGAAACAGC  GGACACTGTA  1500
1501  GGTTGCTGCT  CGCTTCGTGT  GGAGCACATC  AATCTGCACC  CAGAGCTGGA  TGGTCAGGAG  1560
1561  TATGTGGTGG  AGTTTGACTT  CCTGGGAAAG  GACTCAATCA  GATACTATAA  CAAAGTCCCC  1620
1621  GTGGAGAAGC  GAGTTTTTAA  GAACTTACAA  CTATTTATGG  AAAACAAACA  GCCTGAGGAT  1680
1681  GACCTTTTTG  ATAGACTCAA  TACTGGTATC  CTAAATAAAC  ATCTCCAGGA  TCTCATGGAA  1740
1741  GGCTTGACAG  CCAAGGTGTT  CCGTACATAT  AATGCCTCCA  TCACGCTACA  GCAGCAGTTA  1800
1801  AAGGAACTCA  CAGCT  1815

▼ KEYWORD

ID
Family
Coiled coil
Complete proteome
DNA-binding
Isomerase
Reference proteome
Topoisomerase

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Chromosome
Molecular Function
DNA binding
Molecular Function
DNA topoisomerase type I activity
Biological Process
DNA topological change

▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Bot-0142Bos taurus99.50.0 794
LLPS-Hos-1442Homo sapiens98.990.0 792
LLPS-Pap-1007Pan paniscus98.990.0 792
LLPS-Pat-2164Pan troglodytes98.990.0 792
LLPS-Gog-1352Gorilla gorilla98.990.0 792
LLPS-Urm-0041Ursus maritimus98.740.0 788
LLPS-Caf-0801Canis familiaris98.740.0 788
LLPS-Nol-0829Nomascus leucogenys98.740.0 790
LLPS-Sus-0640Sus scrofa98.740.0 790
LLPS-Tut-0222Tursiops truncatus98.740.0 791
LLPS-Aim-0343Ailuropoda melanoleuca98.740.0 789
LLPS-Man-2337Macaca nemestrina98.490.0 788
LLPS-Rhb-0291Rhinopithecus bieti98.490.0 788
LLPS-Mam-0540Macaca mulatta98.490.0 788
LLPS-Aon-1989Aotus nancymaae98.490.0 789
LLPS-Chs-0162Chlorocebus sabaeus98.490.0 789
LLPS-Maf-1880Macaca fascicularis98.490.0 788
LLPS-Mup-0709Mustela putorius furo98.490.0 787
LLPS-Mal-0165Mandrillus leucophaeus98.490.0 788
LLPS-Loa-2967Loxodonta africana98.240.0 786
LLPS-Cap-2837Cavia porcellus98.240.0 786
LLPS-Cea-0878Cercocebus atys98.240.0 788
LLPS-Caj-0741Callithrix jacchus98.240.0 788
LLPS-Paa-1011Papio anubis98.240.0 787
LLPS-Cas-0050Carlito syrichta97.990.0 784
LLPS-Fud-1048Fukomys damarensis97.990.0 785
LLPS-Fec-2542Felis catus97.670.0 865
LLPS-Myl-1935Myotis lucifugus97.670.0 867
LLPS-Otg-0723Otolemur garnettii97.490.0 779
LLPS-Mod-0876Monodelphis domestica97.240.0 780
LLPS-Poa-0572Pongo abelii97.240.0 779
LLPS-Ran-0362Rattus norvegicus97.080.0 862
LLPS-Mum-0700Mus musculus96.890.0 859
LLPS-Dio-2015Dipodomys ordii96.890.0 864
LLPS-Sah-3464Sarcophilus harrisii96.730.0 778
LLPS-Mea-0203Mesocricetus auratus96.690.0 860
LLPS-Orc-3131Oryctolagus cuniculus96.480.0 778
LLPS-Ict-2300Ictidomys tridecemlineatus96.39e-24 110
LLPS-Meg-2160Meleagris gallopavo95.970.0 769
LLPS-Gaga-0563Gallus gallus95.970.0 771
LLPS-Anp-0067Anas platyrhynchos95.720.0 771
LLPS-Fia-0231Ficedula albicollis95.720.0 772
LLPS-Tag-0367Taeniopygia guttata95.470.0 771
LLPS-Ora-1464Ornithorhynchus anatinus95.230.0 766
LLPS-Lac-2401Latimeria chalumnae91.210.0 738
LLPS-Eqc-0178Equus caballus89.980.0 841
LLPS-Leo-1256Lepisosteus oculatus89.20.0 737
LLPS-Scf-2098Scleropages formosus88.440.0 725
LLPS-Pes-2429Pelodiscus sinensis88.240.0 817
LLPS-Orl-0201Oryzias latipes88.190.0 723
LLPS-Dar-0038Danio rerio88.160.0 726
LLPS-Gaa-0071Gasterosteus aculeatus87.690.0 716
LLPS-Scm-0605Scophthalmus maximus87.410.0 715
LLPS-Orn-0149Oreochromis niloticus87.190.0 720
LLPS-Ten-0765Tetraodon nigroviridis87.190.0 716
LLPS-Tar-0854Takifugu rubripes87.190.0 714
LLPS-Pof-2889Poecilia formosa87.150.0 723
LLPS-Xim-1009Xiphophorus maculatus87.150.0 719
LLPS-Asm-1515Astyanax mexicanus86.430.0 711
LLPS-Anc-0092Anolis carolinensis86.130.0 799
LLPS-Icp-0822Ictalurus punctatus84.890.0 703
LLPS-Xet-0888Xenopus tropicalis80.10.0 647
LLPS-Cis-0286Ciona savignyi74.050.0 593
LLPS-Drm-0041Drosophila melanogaster70.810.0 572
LLPS-Cii-0007Ciona intestinalis68.825e-158 470
LLPS-Cae-0577Caenorhabditis elegans66.420.0 540
LLPS-Abg-1221Absidia glauca60.05e-46 176
LLPS-Trv-0179Trichoderma virens54.461e-132 417
LLPS-Scc-0569Schizosaccharomyces cryophilus54.453e-132 414
LLPS-Sac-0024Saccharomyces cerevisiae54.185e-133 414
LLPS-Trr-0678Trichoderma reesei54.074e-134 421
LLPS-Scp-0401Schizosaccharomyces pombe53.443e-129 406
LLPS-Hea-0802Helianthus annuus53.063e-121 386
LLPS-Nia-0032Nicotiana attenuata53.032e-124 395
LLPS-Sol-0382Solanum lycopersicum53.031e-124 396
LLPS-Asg-0037Ashbya gossypii52.913e-125 393
LLPS-Pug-0198Puccinia graminis52.822e-131 416
LLPS-Dac-1194Daucus carota52.788e-117 374
LLPS-Sot-0087Solanum tuberosum52.533e-123 393
LLPS-Pytr-0692Pyrenophora triticirepentis52.496e-127 402
LLPS-Gas-0014Galdieria sulphuraria52.432e-125 395
LLPS-Yal-0350Yarrowia lipolytica52.387e-125 393
LLPS-Map-0654Magnaporthe poae52.365e-126 400
LLPS-Crn-0655Cryptococcus neoformans52.34e-118 380
LLPS-Cogr-0227Colletotrichum graminicola52.232e-126 401
LLPS-Mel-0956Melampsora laricipopulina52.173e-124 396
LLPS-Cus-0295Cucumis sativus52.163e-117 376
LLPS-Prp-1027Prunus persica52.033e-116 371
LLPS-Phn-0072Phaeosphaeria nodorum51.999e-126 399
LLPS-Viv-1208Vitis vinifera51.792e-114 369
LLPS-Pyt-0166Pyrenophora teres51.744e-125 397
LLPS-Lem-1229Leptosphaeria maculans51.741e-125 402
LLPS-Tum-0273Tuber melanosporum51.662e-126 402
LLPS-Cog-0169Colletotrichum gloeosporioides51.621e-123 393
LLPS-Gag-0312Gaeumannomyces graminis51.615e-122 390
LLPS-Blg-0112Blumeria graminis51.614e-126 402
LLPS-Scj-1143Schizosaccharomyces japonicus51.515e-123 390
LLPS-Sem-2233Selaginella moellendorffii51.452e-119 372
LLPS-Kop-0227Komagataella pastoris51.417e-128 401
LLPS-Php-1960Physcomitrella patens51.392e-120 384
LLPS-Beb-1429Beauveria bassiana51.358e-124 394
LLPS-Mua-0764Musa acuminata51.264e-114 367
LLPS-Brr-2053Brassica rapa51.153e-113 366
LLPS-Bro-0855Brassica oleracea51.155e-114 368
LLPS-Brn-2309Brassica napus51.152e-114 368
LLPS-Sei-0917Setaria italica51.146e-118 379
LLPS-Mao-0644Magnaporthe oryzae51.128e-123 392
LLPS-Sob-0391Sorghum bicolor51.012e-117 379
LLPS-Coo-0704Colletotrichum orbiculare50.993e-124 395
LLPS-Pot-0497Populus trichocarpa50.893e-113 365
LLPS-Ors-0740Oryza sativa50.892e-115 372
LLPS-Art-1206Arabidopsis thaliana50.897e-112 363
LLPS-Orbr-0790Oryza brachyantha50.894e-116 374
LLPS-Orr-0809Oryza rufipogon50.892e-115 372
LLPS-Orm-1714Oryza meridionalis50.896e-116 375
LLPS-Ori-1250Oryza indica50.891e-115 373
LLPS-Orb-0471Oryza barthii50.892e-115 372
LLPS-Orni-1203Oryza nivara50.891e-115 373
LLPS-Orgl-0085Oryza glumaepatula50.892e-115 372
LLPS-Arl-0426Arabidopsis lyrata50.897e-108 352
LLPS-Hov-1353Hordeum vulgare50.891e-112 366
LLPS-Put-0273Puccinia triticina50.641e-125 399
LLPS-Orp-0149Oryza punctata50.632e-115 372
LLPS-Tru-1082Triticum urartu50.638e-113 366
LLPS-Zem-0111Zea mays50.632e-117 378
LLPS-Lep-0068Leersia perrieri50.632e-114 370
LLPS-Gor-0232Gossypium raimondii50.381e-112 364
LLPS-Tra-2038Triticum aestivum50.382e-112 365
LLPS-Brd-1783Brachypodium distachyon50.384e-115 371
LLPS-Mae-1784Manihot esculenta50.136e-116 373
LLPS-Glm-0422Glycine max50.133e-114 369
LLPS-Met-0016Medicago truncatula50.132e-114 369
LLPS-Fus-0334Fusarium solani50.122e-120 385
LLPS-Zyt-0133Zymoseptoria tritici50.123e-121 387
LLPS-Nec-0941Neurospora crassa50.121e-120 386
LLPS-Asfu-0136Aspergillus fumigatus49.886e-118 381
LLPS-Asn-0001Aspergillus nidulans49.882e-121 387
LLPS-Coc-1255Corchorus capsularis49.873e-111 361
LLPS-Fuo-0214Fusarium oxysporum49.759e-120 384
LLPS-Ast-0483Aspergillus terreus49.636e-120 383
LLPS-Dos-0835Dothistroma septosporum49.635e-121 387
LLPS-Asni-0175Aspergillus niger49.631e-119 383
LLPS-Aso-0623Aspergillus oryzae49.639e-121 385
LLPS-Asf-0388Aspergillus flavus49.639e-121 385
LLPS-Amt-0088Amborella trichopoda49.626e-112 362
LLPS-Thc-1572Theobroma cacao49.621e-111 362
LLPS-Nef-0103Neosartorya fischeri49.389e-119 380
LLPS-Ved-0262Verticillium dahliae49.383e-109 356
LLPS-Fuv-0179Fusarium verticillioides49.263e-119 382
LLPS-Asc-0022Aspergillus clavatus49.135e-119 381
LLPS-Phv-0993Phaseolus vulgaris48.852e-111 362
LLPS-Chc-0687Chondrus crispus48.323e-113 361
LLPS-Osl-0705Ostreococcus lucimarinus47.811e-98 317
LLPS-Chr-0521Chlamydomonas reinhardtii47.635e-103 333
LLPS-Miv-0739Microbotryum violaceum47.364e-104 343
LLPS-Vir-0484Vigna radiata46.561e-106 345
LLPS-Cym-0044Cyanidioschyzon merolae46.553e-110 353
LLPS-Via-0266Vigna angularis46.314e-105 343
LLPS-Spr-0617Sporisorium reilianum38.721e-79 277
LLPS-Usm-0494Ustilago maydis38.249e-79 275