LLPS-Mum-3555
S100a9

▼ OVERVIEW

Status: Unreviewed
Protein Name: Protein S100-A9; Calgranulin-B; Leukocyte L1 complex heavy chain; Migration inhibitory factor-related protein 14; MRP-14; p14; S100 calcium-binding protein A9
Gene Name: S100a9, Cagb, Mrp14
Ensembl Gene: ENSMUSG00000056071.12
Ensembl Protein: ENSMUSP00000112843.1
Organism: Mus musculus
Taxa ID: 10090
LLPS Type: Client


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
Nucleolus, P-body, Stress granulePredicted from orthologs(View)

▼ FUNCTION

S100A9 is a calcium- and zinc-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. It can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2 and can induce degranulation of neutrophils by a MAPK-dependent mechanism. Predominantly found as calprotectin (S100A8/A9) which has a wide plethora of intra- and extracellular functions. The intracellular functions include: facilitating leukocyte arachidonic acid trafficking and metabolism, modulation of the tubulin-dependent cytoskeleton during migration of phagocytes and activation of the neutrophilic NADPH-oxidase. Activates NADPH-oxidase by facilitating the enzyme complex assembly at the cell membrane, transferring arachidonic acid, an essential cofactor, to the enzyme complex and S100A8 contributes to the enzyme assembly by directly binding to NCF2/P67PHOX. The extracellular functions involve proinflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities. Its proinflammatory activity includes recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to pattern recognition receptors such as Toll-like receptor 4 (TLR4) and receptor for advanced glycation endproducts (AGER). Binding to TLR4 and AGER activates the MAP-kinase and NF-kappa-B signaling pathways resulting in the amplification of the proinflammatory cascade. Has antimicrobial activity towards bacteria and fungi and exerts its antimicrobial activity probably via chelation of Zn(2+) which is essential for microbial growth. Can induce cell death via autophagy and apoptosis and this occurs through the cross-talk of mitochondria and lysosomes via reactive oxygen species (ROS) and the process involves BNIP3. Can regulate neutrophil number and apoptosis by an anti-apoptotic effect; regulates cell survival via ITGAM/ITGB and TLR4 and a signaling mechanism involving MEK-ERK. Its role as an oxidant scavenger has a protective role in preventing exaggerated tissue damage by scavenging oxidants. The iNOS-S100A8/A9 transnitrosylase complex is proposed to direct selective inflammatory stimulus-dependent S-nitrosylation of multiple targets such as GAPDH, NXA5, EZR, MSN and VIM by recognizing a [IL]-x-C-x-x-[DE] motif (By similarity).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MANKAPSQME  RSITTIIDTF  HQYSRKEGHP  DTLSKKEFRQ  MVEAQLATFM  KKEKRNEALI  60
61    NDIMEDLDTN  QDNQLSFEEC  MMLMAKLIFA  CHEKLHENNP  RGHGHSHGKG  CGK  113
Nucleotide CDS Sequence (FASTA)
1     ATGGCCAACA  AAGCACCTTC  TCAGATGGAG  CGCAGCATAA  CCACCATCAT  CGACACCTTC  60
61    CATCAATACT  CTAGGAAGGA  AGGACACCCT  GACACCCTGA  GCAAGAAGGA  ATTCAGACAA  120
121   ATGGTGGAAG  CACAGTTGGC  AACCTTTATG  AAGAAAGAGA  AGAGAAATGA  AGCCCTCATA  180
181   AATGACATCA  TGGAGGACCT  GGACACAAAC  CAGGACAATC  AGCTGAGCTT  TGAGGAGTGT  240
241   ATGATGCTGA  TGGCAAAGTT  GATCTTTGCC  TGTCATGAGA  AGCTGCATGA  GAACAACCCA  300
301   CGTGGGCATG  GCCACAGTCA  TGGCAAAGGC  TGTGGGAAGT  AA  342

▼ KEYWORD

ID
Family
Acetylation
Antimicrobial
Antioxidant
Apoptosis
Autophagy
Calcium
Cell membrane
Chemotaxis
Complete proteome
Cytoplasm
Cytoskeleton
Direct protein sequencing
Immunity
Inflammatory response
Innate immunity
Membrane
Metal-binding
Methylation
Phosphoprotein
Reference proteome
Repeat
Secreted
Zinc

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cell junction
Cellular Component
Cytoplasm
Cellular Component
Cytoskeleton
Cellular Component
Cytosol
Cellular Component
Extracellular space
Cellular Component
Nucleoplasm
Cellular Component
Plasma membrane
Molecular Function
Antioxidant activity
Molecular Function
Arachidonic acid binding
Molecular Function
Calcium ion binding
Molecular Function
Microtubule binding
Molecular Function
RAGE receptor binding
Molecular Function
Toll-like receptor 4 binding
Molecular Function
Zinc ion binding
Biological Process
Actin cytoskeleton reorganization
Biological Process
Activation of cysteine-type endopeptidase activity involved in apoptotic process
Biological Process
Antimicrobial humoral immune response mediated by antimicrobial peptide
Biological Process
Apoptotic process
Biological Process
Astrocyte development
Biological Process
Autophagy
Biological Process
Innate immune response
Biological Process
Leukocyte chemotaxis
Biological Process
Leukocyte migration involved in inflammatory response
Biological Process
Modification of morphology or physiology of other organism
Biological Process
Neutrophil aggregation
Biological Process
Neutrophil chemotaxis
Biological Process
Peptidyl-cysteine S-nitrosylation
Biological Process
Peptidyl-cysteine S-trans-nitrosylation
Biological Process
Positive regulation of blood coagulation
Biological Process
Positive regulation of inflammatory response
Biological Process
Positive regulation of intrinsic apoptotic signaling pathway
Biological Process
Positive regulation of neuron projection development
Biological Process
Positive regulation of peptide secretion
Biological Process
Regulation of integrin biosynthetic process
Biological Process
Regulation of translation

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Ran-2176Rattus norvegicus77.231e-52 168
LLPS-Cap-1828Cavia porcellus65.621e-36 127
LLPS-Maf-1753Macaca fascicularis64.527e-38 130
LLPS-Cea-4340Cercocebus atys64.527e-38 130
LLPS-Mal-4432Mandrillus leucophaeus64.527e-38 130
LLPS-Man-4565Macaca nemestrina64.527e-38 130
LLPS-Mam-3232Macaca mulatta64.527e-38 130
LLPS-Chs-0746Chlorocebus sabaeus63.441e-37 129
LLPS-Paa-0684Papio anubis63.444e-37 128
LLPS-Orc-4368Oryctolagus cuniculus62.771e-35 124
LLPS-Mea-0521Mesocricetus auratus62.382e-40 136
LLPS-Gog-2888Gorilla gorilla62.372e-35 123
LLPS-Hos-1408Homo sapiens62.372e-35 123
LLPS-Pap-1755Pan paniscus62.371e-35 124
LLPS-Pat-0931Pan troglodytes62.371e-35 124
LLPS-Bot-4565Bos taurus61.74e-34 121
LLPS-Rhb-3297Rhinopithecus bieti61.298e-36 124
LLPS-Nol-4164Nomascus leucogenys61.299e-35 122
LLPS-Poa-0072Pongo abelii61.291e-34 121
LLPS-Loa-4020Loxodonta africana61.051e-36 127
LLPS-Ict-0707Ictidomys tridecemlineatus60.876e-33 117
LLPS-Aon-1577Aotus nancymaae60.222e-34 122
LLPS-Sus-4043Sus scrofa59.571e-30 112
LLPS-Otg-3562Otolemur garnettii59.576e-34 120
LLPS-Caj-0502Callithrix jacchus59.141e-33 119
LLPS-Eqc-2229Equus caballus57.892e-35 125
LLPS-Cas-3323Carlito syrichta56.528e-33 117
LLPS-Ova-2783Ovis aries56.382e-30 111
LLPS-Myl-1335Myotis lucifugus55.918e-31 113
LLPS-Aim-3728Ailuropoda melanoleuca55.322e-31 114
LLPS-Urm-1801Ursus maritimus55.323e-31 113
LLPS-Dio-1643Dipodomys ordii55.12e-31 113
LLPS-Caf-3908Canis familiaris54.264e-28 107
LLPS-Ere-0234Erinaceus europaeus53.261e-28 106
LLPS-Mup-2435Mustela putorius furo53.194e-27 104
LLPS-Fec-3751Felis catus53.192e-29 109
LLPS-Tut-2502Tursiops truncatus52.132e-27 103
LLPS-Fud-3450Fukomys damarensis50.09e-28 104
LLPS-Mod-2856Monodelphis domestica44.571e-2188.6
LLPS-Anc-1044Anolis carolinensis42.223e-2084.0
LLPS-Xim-3437Xiphophorus maculatus41.772e-1366.2
LLPS-Pof-2469Poecilia formosa41.772e-1366.2
LLPS-Sah-2192Sarcophilus harrisii41.36e-1981.3
LLPS-Gaga-3149Gallus gallus39.784e-1878.6
LLPS-Tag-1628Taeniopygia guttata39.786e-1878.2
LLPS-Asm-4022Astyanax mexicanus39.777e-1980.9
LLPS-Ten-3472Tetraodon nigroviridis39.771e-1880.1
LLPS-Meg-1223Meleagris gallopavo39.569e-1877.4
LLPS-Orl-1850Oryzias latipes39.242e-1263.5
LLPS-Fia-3805Ficedula albicollis39.133e-1777.8
LLPS-Scf-2115Scleropages formosus38.752e-1162.0
LLPS-Icp-3248Ictalurus punctatus38.646e-1980.9
LLPS-Ora-0742Ornithorhynchus anatinus38.21e-1879.7
LLPS-Scm-2763Scophthalmus maximus37.973e-1160.1
LLPS-Gaa-1871Gasterosteus aculeatus36.369e-1570.1
LLPS-Dar-1570Danio rerio35.964e-1365.5
LLPS-Orn-1200Oreochromis niloticus35.428e-1570.1
LLPS-Anp-0090Anas platyrhynchos35.422e-1571.2
LLPS-Tar-2969Takifugu rubripes35.356e-1262.4
LLPS-Xet-3185Xenopus tropicalis30.771e-1059.7