LLPS-Mum-2643
Brsk2

▼ OVERVIEW

Status: Reviewed
Protein Name: Serine/threonine-protein kinase BRSK2
Gene Name: Brsk2, Kiaa4256, Sada
Ensembl Gene: ENSMUSG00000053046.16
Ensembl Protein: ENSMUSP00000133750.1
Organism: Mus musculus
Taxa ID: 10090
LLPS Type: Others
PDB: 4YOM (B, A) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Postsynaptic density
"...We report the first direct comparison of the proteome of triplicate isolates of mouse and human cortical postsynaptic densities. The mouse postsynaptic density comprised 1556 proteins and the human one 1461."
Mouse cortex23071613

▼ FUNCTION

Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-175 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-261 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmic reticulum (ER) stress.

▼ SEQUENCE

Protein Sequence (FASTA)
1     MTSTGKDGGG  AQHAQYVGPY  RLEKTLGKGQ  TGLVKLGIHC  VTCQKVAIKI  VNREKLSESV  60
61    LMKVEREIAI  LKLIEHPHVL  KLHDVYENKK  YLYLVLEHVS  GGELFDYLVK  KGRLTPKEAR  120
121   KFFRQIISAL  DFCHSHSICH  RDLKPENLLL  DERNNIRIAD  FGMASLQVGD  SLLETSCGSP  180
181   HYACPEVIRG  EKYDGRKADV  WSCGVILFAL  LVGALPFDDD  NLRQLLEKVK  RGVFHMPHFI  240
241   PPDCQSLLRG  MIEVDAARRL  TLEHIQKHIW  YIGGKNEPEP  EQPIPRKVQI  RSLPSLEDID  300
301   PDVLDSMHSL  GCFRDRNKLL  QDLLSEEENQ  EKMIYFLLLD  RKERYPSHED  EDLPPRNEID  360
361   PPRKRVDSPM  LNRHGKRRPE  RKSMEVLSVT  DGGSPVPARR  AIEMAQHGQR  SRSISGASSG  420
421   LSTSPLSSPR  VTPHPSPRGS  PLPTPKGTPV  HTPKESPAGT  PNPTPPSSPS  VGGVPWRTRL  480
481   NSIKNSFLGS  PRFHRRKLQV  PTPEEMSNLT  PESSPELAKK  SWFGNFINLE  KEEQIFVVIK  540
541   DKPLSSIKAD  IVHAFLSIPS  LSHSVISQTS  FRAEYKATGG  PAVFQKPVKF  QVDITYTEGG  600
601   EAQKENGIYS  VTFTLLSGPS  RRFKRVVETI  QAQLLSTHDQ  PSAQHLSDTT  NCMEVMTGRL  660
661   SKCGTPLSNF  FDVIKQLFSD  EKNGQAAQAP  STPAKRSAHG  PLGDSAAAGP  GGDTEYPMGK  720
721   DMAKMGPPAA  RREQP  735
Nucleotide CDS Sequence (FASTA)
1     ATGACATCGA  CGGGGAAGGA  CGGCGGCGGG  GCGCAGCACG  CGCAGTATGT  GGGGCCCTAC  60
61    CGGCTGGAGA  AGACGCTGGG  CAAGGGGCAG  ACAGGCTTGG  TGAAGCTGGG  AATCCACTGT  120
121   GTCACTTGCC  AGAAGGTCGC  CATCAAAATC  GTGAACCGTG  AGAAGCTCAG  TGAGTCGGTG  180
181   CTGATGAAGG  TGGAGCGAGA  GATTGCCATC  TTGAAGCTCA  TCGAGCATCC  ACATGTACTG  240
241   AAGCTGCATG  ACGTCTATGA  AAACAAAAAA  TATTTATACC  TGGTGCTAGA  ACATGTGTCT  300
301   GGGGGAGAGC  TGTTCGACTA  CCTGGTGAAG  AAGGGCCGGC  TGACCCCCAA  GGAGGCCCGC  360
361   AAGTTCTTCC  GGCAGATCAT  CTCTGCACTG  GACTTCTGTC  ACAGCCACTC  CATATGCCAT  420
421   AGAGACTTGA  AGCCAGAGAA  CCTGCTGCTA  GATGAGAGGA  ACAACATCCG  TATTGCAGAC  480
481   TTTGGCATGG  CATCCCTGCA  GGTGGGAGAC  AGCCTGCTGG  AGACCAGCTG  CGGATCTCCA  540
541   CACTATGCCT  GTCCGGAAGT  GATTCGGGGC  GAGAAGTATG  ATGGCCGCAA  GGCAGATGTG  600
601   TGGAGCTGTG  GTGTGATCCT  GTTCGCCTTG  CTGGTGGGGG  CTCTGCCTTT  TGATGATGAC  660
661   AACCTGCGGC  AGTTGCTGGA  GAAGGTCAAG  CGTGGTGTGT  TCCACATGCC  ACACTTTATC  720
721   CCACCAGACT  GCCAGAGTCT  CCTGCGTGGC  ATGATTGAGG  TGGATGCAGC  TCGGCGCCTC  780
781   ACGCTAGAGC  ACATTCAGAA  ACACATATGG  TATATAGGTG  GCAAGAATGA  GCCAGAGCCC  840
841   GAACAGCCCA  TCCCACGCAA  GGTGCAGATC  CGCTCACTAC  CCAGCTTGGA  AGACATTGAC  900
901   CCTGATGTGT  TGGACAGCAT  GCACTCACTG  GGCTGCTTCC  GAGACCGCAA  CAAGCTGCTG  960
961   CAGGATCTGC  TATCTGAGGA  GGAGAATCAG  GAAAAGATGA  TTTATTTCCT  CCTCCTGGAT  1020
1021  CGGAAAGAAC  GGTATCCAAG  CCATGAGGAT  GAGGACCTGC  CCCCCAGGAA  TGAGATAGAC  1080
1081  CCTCCCCGGA  AGCGTGTGGA  TTCCCCGATG  CTGAACCGGC  ATGGCAAGCG  GCGACCTGAG  1140
1141  CGCAAGTCCA  TGGAAGTGCT  CAGTGTGACA  GATGGTGGCT  CCCCAGTGCC  TGCACGGAGA  1200
1201  GCCATTGAGA  TGGCCCAGCA  TGGCCAGAGA  TCTCGATCCA  TCAGTGGTGC  GTCCTCAGGC  1260
1261  CTTTCTACAA  GTCCACTCAG  CAGTCCTCGG  GTGACCCCTC  ACCCCTCACC  AAGGGGTAGT  1320
1321  CCCCTTCCTA  CCCCCAAAGG  GACGCCTGTC  CACACGCCAA  AGGAGAGCCC  AGCTGGCACA  1380
1381  CCCAACCCCA  CACCACCATC  CAGCCCTAGT  GTTGGAGGAG  TGCCCTGGCG  GACACGACTG  1440
1441  AACTCCATCA  AGAACAGCTT  CCTGGGCTCA  CCTCGATTCC  ACCGCCGGAA  ACTCCAAGTT  1500
1501  CCCACGCCAG  AGGAGATGTC  CAACCTGACC  CCAGAATCCT  CTCCAGAGCT  GGCCAAGAAA  1560
1561  TCGTGGTTCG  GGAACTTCAT  CAACCTGGAG  AAGGAGGAGC  AGATCTTTGT  GGTGATCAAG  1620
1621  GACAAGCCCC  TGAGCTCCAT  CAAGGCTGAC  ATCGTTCATG  CCTTCCTGTC  GATCCCCAGC  1680
1681  CTCAGCCACA  GCGTTATTTC  CCAGACAAGC  TTCAGGGCTG  AATACAAGGC  CACAGGGGGC  1740
1741  CCAGCAGTGT  TCCAGAAGCC  GGTCAAGTTC  CAGGTGGACA  TCACCTACAC  TGAGGGCGGA  1800
1801  GAGGCCCAGA  AGGAGAATGG  CATCTACTCA  GTCACATTCA  CTTTACTCTC  AGGCCCCAGT  1860
1861  CGCCGCTTCA  AGAGGGTGGT  GGAGACCATC  CAGGCCCAGC  TGTTAAGCAC  CCATGACCAG  1920
1921  CCATCAGCCC  AGCACCTGTC  AGGAATTATC  CCGAAAAGTT  AA  1962

▼ KEYWORD

ID
Family
3D-structure
Alternative splicing
Apoptosis
ATP-binding
Cell cycle
Cell division
Complete proteome
Cytoplasm
Cytoskeleton
Endoplasmic reticulum
Exocytosis
Kinase
Magnesium
Metal-binding
Mitosis
Neurogenesis
Nucleotide-binding
Phosphoprotein
Reference proteome
Serine/threonine-protein kinase
Transferase
Ubl conjugation

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Centrosome
Cellular Component
Cytoplasm
Cellular Component
Distal axon
Cellular Component
Endoplasmic reticulum
Cellular Component
Nucleus
Cellular Component
Perinuclear region of cytoplasm
Molecular Function
ATP binding
Molecular Function
ATPase binding
Molecular Function
Magnesium ion binding
Molecular Function
Protein kinase binding
Molecular Function
Protein serine/threonine kinase activity
Molecular Function
Tau-protein kinase activity
Biological Process
Actin cytoskeleton reorganization
Biological Process
Axonogenesis
Biological Process
Cell division
Biological Process
ERAD pathway
Biological Process
Establishment of cell polarity
Biological Process
Exocytosis
Biological Process
G2/M transition of mitotic cell cycle
Biological Process
Intracellular signal transduction
Biological Process
Intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
Biological Process
Microtubule cytoskeleton organization involved in establishment of planar polarity
Biological Process
Neuron differentiation
Biological Process
Neuron projection morphogenesis
Biological Process
Peptidyl-serine phosphorylation
Biological Process
Protein phosphorylation
Biological Process
Regulation of axonogenesis
Biological Process
Regulation of insulin secretion involved in cellular response to glucose stimulus
Biological Process
Regulation of neuron projection development

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Mea-1904Mesocricetus auratus99.860.01420
LLPS-Sah-2215Sarcophilus harrisii97.971e-85 292
LLPS-Ict-4406Ictidomys tridecemlineatus96.310.01296
LLPS-Paa-2925Papio anubis95.620.01285
LLPS-Fud-3629Fukomys damarensis95.370.01251
LLPS-Cea-4536Cercocebus atys95.330.01279
LLPS-Mam-4591Macaca mulatta95.160.01147
LLPS-Maf-3134Macaca fascicularis95.080.01283
LLPS-Ora-0749Ornithorhynchus anatinus94.975e-133 399
LLPS-Caf-1862Canis familiaris94.750.01096
LLPS-Eqc-2831Equus caballus94.750.01246
LLPS-Pap-1368Pan paniscus94.490.01184
LLPS-Man-1124Macaca nemestrina94.480.01263
LLPS-Chs-0261Chlorocebus sabaeus94.460.01244
LLPS-Otg-1266Otolemur garnettii94.130.01277
LLPS-Fec-2087Felis catus94.120.01253
LLPS-Poa-0762Pongo abelii93.790.01147
LLPS-Cap-1284Cavia porcellus93.680.01159
LLPS-Meg-2931Meleagris gallopavo93.490.01212
LLPS-Fia-3045Ficedula albicollis93.380.01211
LLPS-Pof-3346Poecilia formosa92.930.0 806
LLPS-Anp-3223Anas platyrhynchos92.830.01216
LLPS-Anc-2055Anolis carolinensis92.690.01087
LLPS-Sus-3672Sus scrofa92.250.01233
LLPS-Hos-4329Homo sapiens92.210.01283
LLPS-Icp-2359Ictalurus punctatus91.810.01155
LLPS-Tar-3259Takifugu rubripes91.50.01118
LLPS-Tag-2963Taeniopygia guttata91.270.01200
LLPS-Scf-2812Scleropages formosus91.090.01173
LLPS-Nol-2269Nomascus leucogenys91.060.01192
LLPS-Leo-3908Lepisosteus oculatus90.920.01204
LLPS-Gaga-3933Gallus gallus90.910.01246
LLPS-Aim-3236Ailuropoda melanoleuca90.30.01112
LLPS-Lac-2561Latimeria chalumnae90.260.0 724
LLPS-Xet-3392Xenopus tropicalis90.210.0 723
LLPS-Orl-3469Oryzias latipes89.730.0 783
LLPS-Scm-2990Scophthalmus maximus89.640.01115
LLPS-Mup-0811Mustela putorius furo89.210.01096
LLPS-Xim-2785Xiphophorus maculatus88.490.01148
LLPS-Rhb-4324Rhinopithecus bieti87.640.01132
LLPS-Orn-3476Oreochromis niloticus86.970.0 728
LLPS-Gaa-0036Gasterosteus aculeatus86.070.0 967
LLPS-Dar-4064Danio rerio85.40.0 733
LLPS-Asm-1148Astyanax mexicanus85.390.01078
LLPS-Ran-2977Rattus norvegicus85.330.01128
LLPS-Mod-1773Monodelphis domestica83.80.0 984
LLPS-Dio-2754Dipodomys ordii83.220.01115
LLPS-Aon-1667Aotus nancymaae83.190.01052
LLPS-Myl-1433Myotis lucifugus82.770.01056
LLPS-Ova-3991Ovis aries82.430.0 646
LLPS-Pat-0479Pan troglodytes82.090.01025
LLPS-Orc-4310Oryctolagus cuniculus81.719e-92 291
LLPS-Mal-3574Mandrillus leucophaeus80.650.01008
LLPS-Gog-2353Gorilla gorilla76.610.01052
LLPS-Drm-2336Drosophila melanogaster76.150.0 584
LLPS-Ten-3632Tetraodon nigroviridis75.320.01038
LLPS-Caj-2553Callithrix jacchus75.140.01003
LLPS-Bot-0880Bos taurus73.40.0 909
LLPS-Cae-0542Caenorhabditis elegans73.020.0 560
LLPS-Loa-3394Loxodonta africana72.180.0 991
LLPS-Cas-2885Carlito syrichta71.930.0 900
LLPS-Cii-0003Ciona intestinalis71.354e-180 539
LLPS-Urm-2178Ursus maritimus71.080.0 930
LLPS-Cis-0958Ciona savignyi54.410.0 658
LLPS-Put-1237Puccinia triticina54.146e-89 305
LLPS-Gas-0506Galdieria sulphuraria53.231e-107 343
LLPS-Chc-0095Chondrus crispus52.238e-90 297
LLPS-Abg-0262Absidia glauca51.852e-106 355
LLPS-Trr-1185Trichoderma reesei50.398e-85 288
LLPS-Tum-1067Tuber melanosporum48.778e-92 300
LLPS-Kop-0822Komagataella pastoris48.752e-96 330
LLPS-Pug-0494Puccinia graminis48.458e-106 353
LLPS-Miv-1599Microbotryum violaceum47.381e-98 334
LLPS-Crn-0764Cryptococcus neoformans47.312e-104 349
LLPS-Yal-1230Yarrowia lipolytica47.181e-94 325
LLPS-Cym-0631Cyanidioschyzon merolae47.126e-102 333
LLPS-Usm-1470Ustilago maydis45.222e-96 330
LLPS-Sem-0026Selaginella moellendorffii44.983e-87 290
LLPS-Art-2601Arabidopsis thaliana44.882e-89 296
LLPS-Mae-2498Manihot esculenta44.681e-88 294
LLPS-Glm-2082Glycine max44.613e-92 304
LLPS-Arl-1065Arabidopsis lyrata44.583e-88 293
LLPS-Gor-0929Gossypium raimondii44.581e-87 294
LLPS-Phv-2505Phaseolus vulgaris44.415e-91 301
LLPS-Scc-0225Schizosaccharomyces cryophilus44.211e-84 285
LLPS-Brn-1367Brassica napus44.191e-88 294
LLPS-Orni-0188Oryza nivara44.183e-89 299
LLPS-Bro-2943Brassica oleracea44.073e-87 290
LLPS-Asg-1531Ashbya gossypii44.039e-89 309
LLPS-Orbr-0158Oryza brachyantha43.883e-89 296
LLPS-Hea-0165Helianthus annuus43.755e-87 289
LLPS-Sei-0362Setaria italica43.671e-85 287
LLPS-Pot-2455Populus trichocarpa43.538e-89 295
LLPS-Dac-0262Daucus carota43.443e-89 296
LLPS-Zem-1597Zea mays43.443e-90 305
LLPS-Hov-1180Hordeum vulgare43.393e-87 292
LLPS-Sol-1999Solanum lycopersicum43.364e-87 290
LLPS-Orb-2279Oryza barthii43.33e-90 299
LLPS-Lep-2156Leersia perrieri43.32e-90 299
LLPS-Orgl-0300Oryza glumaepatula43.35e-90 301
LLPS-Ors-2364Oryza sativa43.31e-90 299
LLPS-Orr-1376Oryza rufipogon43.31e-90 299
LLPS-Tra-2482Triticum aestivum43.14e-87 290
LLPS-Tru-1629Triticum urartu43.18e-86 289
LLPS-Brd-1892Brachypodium distachyon43.023e-87 290
LLPS-Orm-0713Oryza meridionalis43.025e-90 298
LLPS-Ere-0560Erinaceus europaeus42.93e-88 292
LLPS-Mel-1357Melampsora laricipopulina42.895e-98 332
LLPS-Met-1164Medicago truncatula42.824e-86 287
LLPS-Php-1689Physcomitrella patens42.532e-87 292
LLPS-Nia-2028Nicotiana attenuata42.482e-86 288
LLPS-Sob-2229Sorghum bicolor42.451e-89 296
LLPS-Scp-1189Schizosaccharomyces pombe42.198e-85 286
LLPS-Brr-2260Brassica rapa41.843e-88 293
LLPS-Spr-1166Sporisorium reilianum41.534e-94 324
LLPS-Gag-0208Gaeumannomyces graminis41.56e-86 300
LLPS-Pes-1866Pelodiscus sinensis40.919e-85 290
LLPS-Fus-0835Fusarium solani40.752e-85 298