LLPS-Mum-2502
Uba3
▼ OVERVIEW
| Status: | Reviewed |
| Protein Name: | NEDD8-activating enzyme E1 catalytic subunit; NEDD8-activating enzyme E1C; Ubiquitin-activating enzyme E1C; Ubiquitin-like modifier-activating enzyme 3; Ubiquitin-activating enzyme 3 |
| Gene Name: | Uba3, Ube1c |
| Ensembl Gene: | ENSMUSG00000030061.16 |
| Ensembl Protein: | ENSMUSP00000130954.1 |
| Organism: | Mus musculus |
| Taxa ID: | 10090 |
| LLPS Type: | Others |
▼ Classification
Condensates:
| Condensate | Description | Tissue/Cell | PMIDs |
|---|
▼ FUNCTION
| Catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Down-regulates steroid receptor activity. Necessary for cell cycle progression. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | ENSMUST00000164744.7 | ENSMUSP00000130954.1 |
| Ensembl | ENSMUST00000204056.1 | ENSMUSP00000145309.1 |
| Ensembl | ENSMUST00000089287.6 | ENSMUSP00000086701.5 |
| UniProt | Q8C878, UBA3_MOUSE, O88598, Q3TG68, Q9D6B7 | |
| GeneBank | AK048148, AY029181, AF077330, CH466523, BC002002, AK168859, AK159381, AK014433, AK032514 | AAK33015.1, BAE40680.1, BAB29346.1, BAC27905.1, AAH02002.1, BAE35036.1, BAC33258.1, EDK99352.1, AAC27323.1 |
| RefSeq | NM_001111106.2, NM_001301857.1, NM_001301858.1, NM_001301859.1, NM_011666.3, XM_006505913.3, XM_011241290.2 | NP_001104576.1, NP_001288786.1, NP_001288787.1, NP_001288788.1, NP_035796.2, XP_006505976.1, XP_011239592.1 |
| Entrez | 22200 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MADGEEPEKK RRRIEELLAE KMAVDGGCGD TGDWEGRWNH VKKFLERSGP FTHPDFEPST 60 61 ESLQFLLDTC KVLVIGAGGL GCELLKNLAL SGFRQIHVID MDTIDVSNLN RQFLFRPKDV 120 121 GRPKAEVAAE FLNDRVPNCN VVPHFNKIQD FNDTFYRQFH IIVCGLDSII ARRWINGMLI 180 181 SLLNYEDGVL DPSSIVPLID GGTEGFKGNA RVILPGMTAC IECTLELYPP QVNFPMCTIA 240 241 SMPRLPEHCI EYVRMLQWPK EQPFGDGVPL DGDDPEHIQW IFQKSIERAS QYNIRGVTYR 300 301 LTQGVVKRII PAVASTNAVI AAVCATEVFK IATSAYIPLN NYLVFNDVDG LYTYTFEAER 360 361 KENCPACSQL PQNIQFSPSA KLQEVLDYLT NSASLQMKSP AITATLEGKN RTLYLQSVTS 420 421 IEERTRPNLS KTLKELGLVD GQELAVADVT TPQTVLFKLH FT 462 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGGCGGATG GCGAGGAGCC GGAGAAGAAA AGAAGGAGAA TAGAGGAGCT GCTGGCTGAG 60 61 AAAATGGCTG TTGATGGTGG GTGTGGGGAC ACTGGAGACT GGGAAGGTCG CTGGAACCAT 120 121 GTAAAGAAGT TCCTCGAGCG GTCTGGACCC TTCACACACC CCGATTTCGA ACCAAGCACT 180 181 GAATCACTCC AGTTCTTGTT AGATACATGT AAAGTTCTAG TCATTGGAGC TGGTGGCTTA 240 241 GGATGTGAGC TTCTGAAAAA TCTGGCATTA TCTGGTTTTA GACAGATTCA TGTTATAGAC 300 301 ATGGACACTA TAGATGTTTC CAATTTAAAT AGACAGTTTT TATTTAGGCC TAAAGATGTC 360 361 GGAAGACCCA AGGCTGAAGT TGCTGCAGAA TTCCTAAATG ACAGAGTTCC TAACTGCAAC 420 421 GTGGTACCAC ATTTCAACAA GATACAAGAT TTTAACGACA CTTTCTACCG ACAATTTCAT 480 481 ATCATTGTAT GTGGCCTGGA CTCTATCATA GCGAGAAGAT GGATCAATGG AATGCTGATA 540 541 TCTCTTCTAA ATTATGAAGA TGGTGTGTTG GATCCAAGCT CCATTGTACC TTTGATAGAT 600 601 GGGGGGACAG AAGGCTTTAA AGGGAATGCC CGAGTGATTT TGCCTGGAAT GACCGCTTGT 660 661 ATTGAGTGCA CTCTGGAACT TTACCCACCA CAGGTCAATT TCCCCATGTG TACCATTGCA 720 721 TCTATGCCCA GGCTCCCAGA ACACTGTATC GAGTATGTGA GGATGTTGCA ATGGCCTAAA 780 781 GAGCAGCCTT TTGGAGATGG GGTTCCATTA GATGGAGATG ACCCTGAACA TATTCAGTGG 840 841 ATTTTCCAAA AGTCCATAGA GAGAGCATCA CAATATAATA TTAGAGGCGT TACCTACAGA 900 901 CTCACTCAAG GGGTGGTAAA ACGAATCATT CCTGCAGTAG CTTCTACAAA TGCAGTCATT 960 961 GCAGCTGTGT GTGCCACTGA GGTTTTCAAG ATAGCTACAA GTGCGTACAT TCCCCTTAAT 1020 1021 AACTACCTGG TATTCAATGA TGTAGATGGG CTGTACACTT ACACGTTTGA AGCAGAGAGA 1080 1081 AAGGAAAACT GTCCAGCATG TAGCCAACTT CCTCAAAACA TTCAGTTTTC CCCATCAGCT 1140 1141 AAACTACAGG AGGTCTTAGA CTACCTAACC AACAGTGCTT CTCTGCAAAT GAAGTCTCCG 1200 1201 GCTATCACAG CCACATTAGA GGGGAAGAAC AGGACACTTT ACTTACAGTC AGTAACGTCT 1260 1261 ATTGAAGAAC GAACCAGGCC CAATCTTTCC AAAACATTAA AAGAACTGGG ACTAGTTGAT 1320 1321 GGACAAGAAC TGGCTGTTGC TGATGTCACT ACACCACAGA CTGTACTATT CAAACTTCAT 1380 1381 TTTACTTAA 1389 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Cytoplasm | |
Cellular Component | Nucleus | |
Cellular Component | Protein-containing complex | |
Molecular Function | ATP binding | |
Molecular Function | Identical protein binding | |
Molecular Function | NEDD8 activating enzyme activity | |
Molecular Function | Nuclear receptor binding | |
Molecular Function | Protein heterodimerization activity | |
Biological Process | Endomitotic cell cycle | |
Biological Process | Mitotic cell cycle | |
Biological Process | Negative regulation of transcription, DNA-templated | |
Biological Process | Protein modification by small protein conjugation | |
Biological Process | Protein neddylation | |
Biological Process | Regulation of cell cycle |
▼ ANNOTATION
| Mutation | ||||
| CGAP | ||||
| Physicochemical | ||||
| Compute pI/Mw | AAindex | |||
| Function | ||||
| PIRSF | ||||
| Localization | ||||
| COMPARTMENTS | ||||
| Expression | ||||
| ArrayExpress | GXD | TISSUES | ||
| Element | ||||
| miRWalk | RAID | microRNA.org | ||
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Mea-3997 | Mesocricetus auratus | 100.0 | 8e-61 | 208 |
| LLPS-Ran-4413 | Rattus norvegicus | 99.78 | 0.0 | 922 |
| LLPS-Hos-4884 | Homo sapiens | 99.35 | 0.0 | 920 |
| LLPS-Pat-1409 | Pan troglodytes | 99.35 | 0.0 | 920 |
| LLPS-Man-4519 | Macaca nemestrina | 99.35 | 0.0 | 920 |
| LLPS-Rhb-1888 | Rhinopithecus bieti | 99.35 | 0.0 | 920 |
| LLPS-Maf-1738 | Macaca fascicularis | 99.35 | 0.0 | 920 |
| LLPS-Cea-4156 | Cercocebus atys | 99.35 | 0.0 | 920 |
| LLPS-Paa-0522 | Papio anubis | 99.35 | 0.0 | 920 |
| LLPS-Mam-2423 | Macaca mulatta | 99.32 | 0.0 | 915 |
| LLPS-Aon-4156 | Aotus nancymaae | 99.13 | 0.0 | 919 |
| LLPS-Gog-4390 | Gorilla gorilla | 99.13 | 0.0 | 916 |
| LLPS-Caj-0791 | Callithrix jacchus | 99.13 | 0.0 | 919 |
| LLPS-Caf-2636 | Canis familiaris | 99.09 | 0.0 | 915 |
| LLPS-Fec-4813 | Felis catus | 98.92 | 0.0 | 917 |
| LLPS-Orc-3614 | Oryctolagus cuniculus | 98.92 | 0.0 | 917 |
| LLPS-Poa-1450 | Pongo abelii | 98.92 | 0.0 | 916 |
| LLPS-Eqc-0897 | Equus caballus | 98.92 | 0.0 | 916 |
| LLPS-Chs-4549 | Chlorocebus sabaeus | 98.92 | 0.0 | 919 |
| LLPS-Sus-2739 | Sus scrofa | 98.92 | 0.0 | 916 |
| LLPS-Urm-3569 | Ursus maritimus | 98.87 | 0.0 | 912 |
| LLPS-Otg-2467 | Otolemur garnettii | 98.7 | 0.0 | 915 |
| LLPS-Mup-0422 | Mustela putorius furo | 98.65 | 0.0 | 907 |
| LLPS-Cas-3286 | Carlito syrichta | 98.64 | 0.0 | 912 |
| LLPS-Bot-2282 | Bos taurus | 98.64 | 0.0 | 911 |
| LLPS-Aim-2127 | Ailuropoda melanoleuca | 98.41 | 0.0 | 910 |
| LLPS-Myl-3680 | Myotis lucifugus | 98.27 | 0.0 | 909 |
| LLPS-Fud-3781 | Fukomys damarensis | 97.96 | 0.0 | 907 |
| LLPS-Loa-2788 | Loxodonta africana | 97.64 | 0.0 | 908 |
| LLPS-Ova-3369 | Ovis aries | 97.53 | 0.0 | 904 |
| LLPS-Ict-3856 | Ictidomys tridecemlineatus | 95.92 | 0.0 | 877 |
| LLPS-Pap-2208 | Pan paniscus | 95.69 | 0.0 | 875 |
| LLPS-Mal-2535 | Mandrillus leucophaeus | 95.69 | 0.0 | 873 |
| LLPS-Sah-3212 | Sarcophilus harrisii | 95.46 | 0.0 | 888 |
| LLPS-Mod-3346 | Monodelphis domestica | 95.45 | 0.0 | 890 |
| LLPS-Ora-2119 | Ornithorhynchus anatinus | 93.72 | 0.0 | 881 |
| LLPS-Dio-1779 | Dipodomys ordii | 93.51 | 0.0 | 847 |
| LLPS-Lac-4061 | Latimeria chalumnae | 91.16 | 0.0 | 863 |
| LLPS-Pes-3812 | Pelodiscus sinensis | 90.93 | 0.0 | 853 |
| LLPS-Anp-3307 | Anas platyrhynchos | 90.91 | 0.0 | 858 |
| LLPS-Anc-2517 | Anolis carolinensis | 90.7 | 0.0 | 853 |
| LLPS-Gaga-3808 | Gallus gallus | 90.48 | 0.0 | 851 |
| LLPS-Nol-2093 | Nomascus leucogenys | 90.48 | 0.0 | 818 |
| LLPS-Meg-1821 | Meleagris gallopavo | 90.26 | 0.0 | 853 |
| LLPS-Xet-0435 | Xenopus tropicalis | 89.82 | 0.0 | 846 |
| LLPS-Fia-0889 | Ficedula albicollis | 89.61 | 0.0 | 852 |
| LLPS-Tag-2292 | Taeniopygia guttata | 89.39 | 0.0 | 850 |
| LLPS-Leo-2650 | Lepisosteus oculatus | 88.44 | 0.0 | 840 |
| LLPS-Icp-3922 | Ictalurus punctatus | 87.07 | 0.0 | 818 |
| LLPS-Dar-2358 | Danio rerio | 87.05 | 0.0 | 830 |
| LLPS-Asm-1082 | Astyanax mexicanus | 86.68 | 0.0 | 768 |
| LLPS-Scm-3498 | Scophthalmus maximus | 86.65 | 0.0 | 809 |
| LLPS-Pof-4129 | Poecilia formosa | 86.2 | 0.0 | 809 |
| LLPS-Ten-3076 | Tetraodon nigroviridis | 86.17 | 0.0 | 808 |
| LLPS-Orn-1277 | Oreochromis niloticus | 85.97 | 0.0 | 801 |
| LLPS-Scf-2362 | Scleropages formosus | 85.52 | 0.0 | 810 |
| LLPS-Gaa-2927 | Gasterosteus aculeatus | 85.26 | 0.0 | 814 |
| LLPS-Tar-0537 | Takifugu rubripes | 85.03 | 0.0 | 796 |
| LLPS-Orl-1902 | Oryzias latipes | 83.45 | 0.0 | 795 |
| LLPS-Cap-4472 | Cavia porcellus | 82.09 | 0.0 | 601 |
| LLPS-Xim-1713 | Xiphophorus maculatus | 81.86 | 0.0 | 794 |
| LLPS-Cis-1755 | Ciona savignyi | 68.6 | 0.0 | 652 |
| LLPS-Cii-0794 | Ciona intestinalis | 68.33 | 0.0 | 655 |
| LLPS-Drm-2139 | Drosophila melanogaster | 63.87 | 0.0 | 570 |
| LLPS-Map-1291 | Magnaporthe poae | 54.44 | 5e-156 | 457 |
| LLPS-Kop-0930 | Komagataella pastoris | 54.41 | 9e-134 | 396 |
| LLPS-Fuv-1566 | Fusarium verticillioides | 54.4 | 3e-142 | 419 |
| LLPS-Mao-1491 | Magnaporthe oryzae | 54.19 | 2e-159 | 465 |
| LLPS-Fuo-1454 | Fusarium oxysporum | 54.13 | 2e-142 | 420 |
| LLPS-Trr-0551 | Trichoderma reesei | 53.6 | 2e-143 | 422 |
| LLPS-Nec-0859 | Neurospora crassa | 53.33 | 1e-152 | 448 |
| LLPS-Cae-1927 | Caenorhabditis elegans | 53.27 | 2e-153 | 451 |
| LLPS-Mel-1469 | Melampsora laricipopulina | 53.06 | 2e-154 | 453 |
| LLPS-Gag-0576 | Gaeumannomyces graminis | 53.01 | 3e-154 | 452 |
| LLPS-Gas-0914 | Galdieria sulphuraria | 53.01 | 1e-165 | 482 |
| LLPS-Fus-1619 | Fusarium solani | 52.33 | 2e-156 | 459 |
| LLPS-Coo-0916 | Colletotrichum orbiculare | 52.16 | 6e-165 | 480 |
| LLPS-Spr-1370 | Sporisorium reilianum | 51.98 | 7e-156 | 456 |
| LLPS-Ved-0779 | Verticillium dahliae | 51.96 | 2e-150 | 442 |
| LLPS-Beb-1443 | Beauveria bassiana | 51.95 | 3e-156 | 457 |
| LLPS-Cogr-1193 | Colletotrichum graminicola | 51.87 | 4e-156 | 457 |
| LLPS-Trv-0713 | Trichoderma virens | 51.75 | 2e-158 | 463 |
| LLPS-Yal-1372 | Yarrowia lipolytica | 51.46 | 1e-144 | 428 |
| LLPS-Ast-1449 | Aspergillus terreus | 51.29 | 7e-152 | 446 |
| LLPS-Asni-0086 | Aspergillus niger | 51.29 | 4e-150 | 441 |
| LLPS-Miv-0124 | Microbotryum violaceum | 51.13 | 4e-150 | 442 |
| LLPS-Usm-1328 | Ustilago maydis | 51.05 | 6e-151 | 443 |
| LLPS-Pug-1246 | Puccinia graminis | 51.04 | 5e-143 | 426 |
| LLPS-Put-0622 | Puccinia triticina | 51.03 | 9e-150 | 445 |
| LLPS-Asfu-1252 | Aspergillus fumigatus | 50.94 | 1e-148 | 437 |
| LLPS-Cog-1170 | Colletotrichum gloeosporioides | 50.92 | 6e-159 | 466 |
| LLPS-Blg-1421 | Blumeria graminis | 50.71 | 2e-124 | 374 |
| LLPS-Tum-1415 | Tuber melanosporum | 50.65 | 2e-139 | 414 |
| LLPS-Amt-2292 | Amborella trichopoda | 50.37 | 1e-129 | 390 |
| LLPS-Nef-1030 | Neosartorya fischeri | 50.23 | 1e-147 | 435 |
| LLPS-Asc-0116 | Aspergillus clavatus | 50.12 | 7e-146 | 431 |
| LLPS-Php-2344 | Physcomitrella patens | 49.88 | 4e-137 | 409 |
| LLPS-Dac-1999 | Daucus carota | 49.88 | 2e-128 | 387 |
| LLPS-Met-0344 | Medicago truncatula | 49.88 | 4e-129 | 389 |
| LLPS-Phn-1432 | Phaeosphaeria nodorum | 49.75 | 3e-135 | 403 |
| LLPS-Glm-1423 | Glycine max | 49.75 | 2e-129 | 389 |
| LLPS-Lem-1393 | Leptosphaeria maculans | 49.66 | 5e-150 | 442 |
| LLPS-Chc-1170 | Chondrus crispus | 49.64 | 3e-143 | 425 |
| LLPS-Zyt-0491 | Zymoseptoria tritici | 49.53 | 2e-143 | 424 |
| LLPS-Ors-1739 | Oryza sativa | 49.5 | 4e-130 | 391 |
| LLPS-Ori-1808 | Oryza indica | 49.5 | 4e-130 | 391 |
| LLPS-Sob-2080 | Sorghum bicolor | 49.38 | 2e-130 | 392 |
| LLPS-Chr-0929 | Chlamydomonas reinhardtii | 49.29 | 3e-129 | 389 |
| LLPS-Sei-0268 | Setaria italica | 49.26 | 4e-129 | 391 |
| LLPS-Tra-2444 | Triticum aestivum | 49.25 | 8e-129 | 388 |
| LLPS-Bro-0226 | Brassica oleracea | 49.13 | 2e-128 | 387 |
| LLPS-Thc-2051 | Theobroma cacao | 49.11 | 2e-125 | 379 |
| LLPS-Pyt-1163 | Pyrenophora teres | 49.08 | 1e-147 | 436 |
| LLPS-Sem-0953 | Selaginella moellendorffii | 49.05 | 5e-136 | 406 |
| LLPS-Via-1047 | Vigna angularis | 49.01 | 2e-123 | 374 |
| LLPS-Cus-2029 | Cucumis sativus | 49.0 | 1e-124 | 377 |
| LLPS-Brd-0447 | Brachypodium distachyon | 49.0 | 9e-126 | 380 |
| LLPS-Crn-0650 | Cryptococcus neoformans | 48.94 | 3e-139 | 414 |
| LLPS-Scs-0635 | Sclerotinia sclerotiorum | 48.94 | 3e-136 | 407 |
| LLPS-Phv-0149 | Phaseolus vulgaris | 48.77 | 4e-127 | 384 |
| LLPS-Coc-1913 | Corchorus capsularis | 48.74 | 2e-124 | 377 |
| LLPS-Abg-1028 | Absidia glauca | 48.62 | 3e-130 | 393 |
| LLPS-Orp-1124 | Oryza punctata | 48.56 | 5e-130 | 391 |
| LLPS-Hea-2621 | Helianthus annuus | 48.54 | 5e-124 | 376 |
| LLPS-Prp-2514 | Prunus persica | 48.51 | 1e-125 | 380 |
| LLPS-Brr-2748 | Brassica rapa | 48.39 | 1e-124 | 377 |
| LLPS-Art-3054 | Arabidopsis thaliana | 48.39 | 2e-124 | 377 |
| LLPS-Pytr-1290 | Pyrenophora triticirepentis | 48.39 | 5e-145 | 429 |
| LLPS-Brn-2838 | Brassica napus | 48.39 | 3e-124 | 377 |
| LLPS-Mua-0235 | Musa acuminata | 48.39 | 1e-123 | 375 |
| LLPS-Mae-2663 | Manihot esculenta | 48.38 | 2e-127 | 384 |
| LLPS-Lep-1350 | Leersia perrieri | 48.32 | 1e-127 | 385 |
| LLPS-Osl-0466 | Ostreococcus lucimarinus | 48.15 | 3e-124 | 376 |
| LLPS-Sol-1765 | Solanum lycopersicum | 48.14 | 8e-124 | 375 |
| LLPS-Arl-2166 | Arabidopsis lyrata | 48.14 | 1e-124 | 377 |
| LLPS-Aso-1226 | Aspergillus oryzae | 47.99 | 6e-141 | 419 |
| LLPS-Sot-2149 | Solanum tuberosum | 47.89 | 1e-123 | 375 |
| LLPS-Hov-0908 | Hordeum vulgare | 47.84 | 2e-125 | 380 |
| LLPS-Viv-2507 | Vitis vinifera | 47.56 | 8e-124 | 375 |
| LLPS-Gor-0499 | Gossypium raimondii | 47.49 | 4e-120 | 365 |
| LLPS-Orbr-2269 | Oryza brachyantha | 47.36 | 4e-123 | 374 |
| LLPS-Orm-1077 | Oryza meridionalis | 46.77 | 2e-116 | 355 |
| LLPS-Pot-0484 | Populus trichocarpa | 46.59 | 3e-123 | 374 |
| LLPS-Vir-1353 | Vigna radiata | 46.43 | 2e-94 | 296 |
| LLPS-Tru-1878 | Triticum urartu | 45.82 | 5e-90 | 288 |
| LLPS-Nia-1299 | Nicotiana attenuata | 45.73 | 2e-44 | 169 |
| LLPS-Dos-1104 | Dothistroma septosporum | 45.52 | 5e-133 | 398 |
| LLPS-Zem-1528 | Zea mays | 45.12 | 1e-123 | 376 |
| LLPS-Asg-0553 | Ashbya gossypii | 44.97 | 3e-85 | 271 |
| LLPS-Orr-1157 | Oryza rufipogon | 44.55 | 2e-111 | 343 |
| LLPS-Orb-0892 | Oryza barthii | 44.55 | 2e-111 | 343 |
| LLPS-Orni-1852 | Oryza nivara | 44.55 | 2e-111 | 343 |
| LLPS-Orgl-1260 | Oryza glumaepatula | 44.55 | 2e-111 | 344 |
| LLPS-Org-1552 | Oryza glaberrima | 44.12 | 3e-44 | 169 |
| LLPS-Scj-1051 | Schizosaccharomyces japonicus | 43.72 | 1e-119 | 364 |
| LLPS-Scp-1023 | Schizosaccharomyces pombe | 43.44 | 4e-115 | 352 |
| LLPS-Asf-1296 | Aspergillus flavus | 43.33 | 4e-101 | 315 |
| LLPS-Asn-0635 | Aspergillus nidulans | 43.06 | 4e-117 | 355 |
| LLPS-Sac-1657 | Saccharomyces cerevisiae | 42.68 | 1e-81 | 261 |
| LLPS-Scc-1489 | Schizosaccharomyces cryophilus | 42.66 | 1e-111 | 344 |
| LLPS-Cym-0672 | Cyanidioschyzon merolae | 36.32 | 2e-76 | 252 |