DrLLPS - Data resource of LLPS

Overview
Property
Classification
Function
Cross Reference
Sequence
GO
Annotation
Orthology
TOP

Disorder▼
- IUPred2A
Domain▼
PTM▼
Variation▼
Mutation▼
- CGAP
Interaction▼
- HINT
- RISE
- RAIN
- Mentha
- IID
- MIST
Disease▼
Drug▼
Physicochemical▼
- Compute pI/Mw
- AAindex
Function▼
- CGDB
- PIRSF
Proteomics▼
Structure▼
Localization▼
- COMPARTMENTS
Expression▼
Element▼
Methylation▼

LLPS-Mum-1531
Nudt21

▼ OVERVIEW


Status:	Reviewed
Protein Name:	Cleavage and polyadenylation specificity factor subunit 5; Nucleoside diphosphate-linked moiety X motif 21; Nudix motif 21; Nudix hydrolase 21
Gene Name:	Nudt21, Cpsf5
Ensembl Gene:	ENSMUSG00000031754.10
Ensembl Protein:	ENSMUSP00000148500.1
Organism:	Mus musculus
Taxa ID:	10090
LLPS Type:	Others

▼ PROPERTY

——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:

Condensate	Description	Tissue/Cell	PMIDs

▼ FUNCTION

Component of the cleavage factor Im (CFIm) complex that functions as an activator of the pre-mRNA 3'-end cleavage and polyadenylation processing required for the maturation of pre-mRNA into functional mRNAs. CFIm contributes to the recruitment of multiprotein complexes on specific sequences on the pre-mRNA 3'-end, so called cleavage and polyadenylation signals (pA signals). Most pre-mRNAs contain multiple pA signals, resulting in alternative cleavage and polyadenylation (APA) producing mRNAs with variable 3'-end formation. The CFIm complex acts as a key regulator of cleavage and polyadenylation site choice during APA through its binding to 5'-UGUA-3' elements localized in the 3'-untranslated region (UTR) for a huge number of pre-mRNAs. NUDT21/CPSF5 activates indirectly the mRNA 3'-processing machinery by recruiting CPSF6 and/or CPSF7. Binds to 5'-UGUA-3' elements localized upstream of pA signals that act as enhancers of pre-mRNA 3'-end processing. The homodimer mediates simultaneous sequence-specific recognition of two 5'-UGUA-3' elements within the pre-mRNA (By similarity). Plays a role in somatic cell fate transitions and pluripotency by regulating widespread changes in gene expression through an APA-dependent function(PubMed:29249356). Binds to chromatin (PubMed:18032416). Binds to, but does not hydrolyze mono- and di-adenosine nucleotides (By similarity).

▼ CROSS REFERENCE

Database	Nucleotide ID	Protein ID
Ensembl	ENSMUST00000212911.1	ENSMUSP00000148500.1
Ensembl	ENSMUST00000034204.10	ENSMUSP00000034204.9
Ensembl	ENSMUST00000212981.1	ENSMUSP00000148597.1
Ensembl	ENSMUST00000212622.1	ENSMUSP00000148485.1
UniProt		Q9CQF3, CPSF5_MOUSE, Q3UJK1
GeneBank	BC008270, AK011688, AK147061, BC090834, AK019433, AK146419, AK160147	BAB27778.1, BAE27645.1, BAB31718.1, AAH90834.1, AAH08270.1, BAE27154.1, BAE35655.1
RefSeq	NM_026623.3	NP_080899.1
Entrez	68219

▼ SEQUENCE

Protein Sequence (FASTA)
1 MSVVPPNRSQ TGWPRGVNQF GNKYIQQTKP LTLERTINLY PLTNYTFGTK EPLYEKDSSV 60 61 AARFQRMREE FDKIGMRRTV EGVLIVHEHR LPHVLLLQLG TTFFKLPGGE LNPGEDEVEG 120 121 LKRLMTEILG RQDGVLQDWV IDDCIGNWWR PNFEPPQYPY IPAHITKPKE HKKLFLVQLQ 180 181 EKALFAVPKN YKLVAAPLFE LYDNAPGYGP IISSLPQLLS RFNFIYN 227
Nucleotide CDS Sequence (FASTA)
1 ATGTCTGTGG TGCCGCCCAA TCGCTCGCAG ACGGGCTGGC CCCGGGGGGT CAACCAGTTC 60 61 GGCAACAAGT ACATCCAGCA GACCAAGCCC CTCACCCTGG AGCGCACCAT TAATCTGTAC 120 121 CCGCTTACCA ATTATACTTT TGGTACAAAG GAGCCCCTCT ATGAGAAGGA CAGCTCTGTT 180 181 GCAGCCAGAT TTCAGCGCAT GAGGGAGGAA TTTGATAAGA TTGGGATGAG AAGGACTGTA 240 241 GAAGGGGTTC TGATTGTTCA TGAACACCGC CTGCCCCACG TGCTCCTGCT GCAGCTGGGG 300 301 ACAACTTTCT TCAAATTACC TGGTGGGGAA CTTAACCCAG GAGAAGATGA AGTTGAAGGA 360 361 CTAAAACGCT TAATGACAGA GATACTTGGT CGTCAAGATG GAGTCCTGCA AGACTGGGTC 420 421 ATTGATGACT GCATTGGGAA CTGGTGGAGA CCAAATTTTG AACCTCCTCA GTATCCGTAT 480 481 ATTCCTGCAC ATATAACAAA ACCCAAGGAA CATAAGAAGT TGTTTCTGGT TCAGCTTCAA 540 541 GAGAAAGCCT TGTTTGCAGT CCCTAAAAAT TACAAGCTGG TAGCTGCACC ATTGTTTGAG 600 601 CTGTATGACA ATGCACCGGG GTATGGACCC ATCATTTCTA GTCTTCCTCA GCTGCTGAGC 660 661 AGGTTCAATT TTATATACAA CTGA 684

▼ KEYWORD

ID	Family
KW-0007	Acetylation
KW-0181	Complete proteome
KW-0963	Cytoplasm
KW-0221	Differentiation
KW-0488	Methylation
KW-0507	mRNA processing
KW-0539	Nucleus
KW-0597	Phosphoprotein
KW-1185	Reference proteome
KW-0694	RNA-binding

▼ GENE ONTOLOGY

ID	Classification	Description
GO:0005813	Cellular Component	Centrosome
GO:0005737	Cellular Component	Cytoplasm
GO:0005815	Cellular Component	Microtubule organizing center
GO:0005847	Cellular Component	MRNA cleavage and polyadenylation specificity factor complex
GO:0005849	Cellular Component	MRNA cleavage factor complex
GO:0016604	Cellular Component	Nuclear body
GO:0005634	Cellular Component	Nucleus
GO:0042382	Cellular Component	Paraspeckles
GO:0017091	Molecular Function	AU-rich element binding
GO:0003682	Molecular Function	Chromatin binding
GO:0042826	Molecular Function	Histone deacetylase binding
GO:0016787	Molecular Function	Hydrolase activity
GO:0042802	Molecular Function	Identical protein binding
GO:0003729	Molecular Function	MRNA binding
GO:0042803	Molecular Function	Protein homodimerization activity
GO:0030154	Biological Process	Cell differentiation
GO:1990120	Biological Process	Messenger ribonucleoprotein complex assembly
GO:0110104	Biological Process	MRNA alternative polyadenylation
GO:0006379	Biological Process	MRNA cleavage
GO:0006378	Biological Process	MRNA polyadenylation
GO:0006397	Biological Process	MRNA processing
GO:0031439	Biological Process	Positive regulation of mRNA cleavage
GO:1900365	Biological Process	Positive regulation of mRNA polyadenylation
GO:2000975	Biological Process	Positive regulation of pro-B cell differentiation
GO:2000738	Biological Process	Positive regulation of stem cell differentiation
GO:0010608	Biological Process	Posttranscriptional regulation of gene expression
GO:0098789	Biological Process	Pre-mRNA cleavage required for polyadenylation
GO:0051290	Biological Process	Protein heterotetramerization
GO:0051262	Biological Process	Protein tetramerization

▼ KEGG

mmu:68219

▼ ANNOTATION

Disorder
IUPred2A

Domain
SMART	Pfam	InterPro	CDD	PROSITE
Gene3D

PTM
EPSD	PLMD	PhosphoSitePlus	dbPTM	BioGRID
mUbiSiDa	PTMcode

Mutation
CGAP

Interaction
HINT	RISE	RAIN	Mentha	IID
MIST

Physicochemical
Compute pI/Mw	AAindex

Function
CGDB	PIRSF

Localization
COMPARTMENTS

Expression
ArrayExpress	GXD	TISSUES

Element
miRWalk	RAID	microRNA.org

▼ ORTHOLOGY

DrLLPS ID	Organism	Identity	E-value	Score
LLPS-Myl-2361	Myotis lucifugus	100.0	2e-167	468
LLPS-Mup-1785	Mustela putorius furo	100.0	2e-167	468
LLPS-Ere-0158	Erinaceus europaeus	100.0	2e-167	468
LLPS-Ran-3031	Rattus norvegicus	100.0	2e-167	468
LLPS-Dio-3006	Dipodomys ordii	100.0	2e-167	468
LLPS-Tut-1637	Tursiops truncatus	100.0	2e-167	468
LLPS-Fud-2283	Fukomys damarensis	100.0	2e-167	468
LLPS-Sah-0490	Sarcophilus harrisii	100.0	2e-167	468
LLPS-Mea-4435	Mesocricetus auratus	100.0	2e-167	468
LLPS-Cas-1956	Carlito syrichta	100.0	2e-167	468
LLPS-Eqc-3955	Equus caballus	100.0	2e-167	468
LLPS-Caf-0767	Canis familiaris	100.0	2e-167	468
LLPS-Orc-3518	Oryctolagus cuniculus	100.0	2e-167	468
LLPS-Otg-1270	Otolemur garnettii	100.0	2e-167	468
LLPS-Loa-3426	Loxodonta africana	100.0	2e-167	468
LLPS-Fec-0941	Felis catus	100.0	2e-167	468
LLPS-Mod-2512	Monodelphis domestica	100.0	2e-167	468
LLPS-Urm-2024	Ursus maritimus	100.0	2e-167	468
LLPS-Ova-3068	Ovis aries	99.56	1e-166	466
LLPS-Aim-1134	Ailuropoda melanoleuca	99.56	1e-164	461
LLPS-Mal-1480	Mandrillus leucophaeus	99.56	1e-166	466
LLPS-Paa-1763	Papio anubis	99.56	1e-166	466
LLPS-Bot-0986	Bos taurus	99.56	1e-166	466
LLPS-Caj-1310	Callithrix jacchus	99.56	1e-166	466
LLPS-Gog-2851	Gorilla gorilla	99.56	1e-166	466
LLPS-Sus-3064	Sus scrofa	99.56	7e-167	467
LLPS-Cea-1264	Cercocebus atys	99.56	1e-166	466
LLPS-Aon-0093	Aotus nancymaae	99.56	1e-166	466
LLPS-Chs-2819	Chlorocebus sabaeus	99.56	1e-166	466
LLPS-Maf-0315	Macaca fascicularis	99.56	1e-166	466
LLPS-Nol-2000	Nomascus leucogenys	99.56	1e-166	466
LLPS-Ict-2105	Ictidomys tridecemlineatus	99.56	1e-166	466
LLPS-Mam-1178	Macaca mulatta	99.56	1e-166	466
LLPS-Poa-1865	Pongo abelii	99.56	1e-166	466
LLPS-Rhb-2838	Rhinopithecus bieti	99.56	1e-166	466
LLPS-Fia-2963	Ficedula albicollis	99.56	2e-166	466
LLPS-Man-2949	Macaca nemestrina	99.56	1e-166	466
LLPS-Hos-2016	Homo sapiens	99.56	1e-166	466
LLPS-Pat-1085	Pan troglodytes	99.56	1e-166	466
LLPS-Gaga-0789	Gallus gallus	99.55	5e-161	461
LLPS-Ora-0943	Ornithorhynchus anatinus	99.52	1e-150	426
LLPS-Meg-1049	Meleagris gallopavo	99.47	1e-135	386
LLPS-Pes-3023	Pelodiscus sinensis	99.38	5e-114	330
LLPS-Xet-1582	Xenopus tropicalis	99.12	6e-166	464
LLPS-Lac-0648	Latimeria chalumnae	99.12	3e-166	465
LLPS-Pap-1257	Pan paniscus	99.12	5e-164	459
LLPS-Anc-0351	Anolis carolinensis	98.68	7e-165	462
LLPS-Cap-0649	Cavia porcellus	98.24	1e-161	453
LLPS-Leo-2685	Lepisosteus oculatus	97.81	7e-162	454
LLPS-Dar-1314	Danio rerio	97.81	1e-162	456
LLPS-Scf-1609	Scleropages formosus	97.37	6e-162	456
LLPS-Anp-3343	Anas platyrhynchos	97.31	7e-131	374
LLPS-Asm-3382	Astyanax mexicanus	96.93	1e-160	451
LLPS-Tag-1428	Taeniopygia guttata	96.48	3e-161	452
LLPS-Icp-3441	Ictalurus punctatus	95.56	1e-155	439
LLPS-Tar-0829	Takifugu rubripes	94.76	1e-156	441
LLPS-Ten-0725	Tetraodon nigroviridis	94.76	8e-157	441
LLPS-Xim-2910	Xiphophorus maculatus	94.32	3e-154	435
LLPS-Pof-2484	Poecilia formosa	94.32	1e-154	436
LLPS-Orn-1214	Oreochromis niloticus	94.3	9e-154	433
LLPS-Orl-2409	Oryzias latipes	93.89	9e-154	434
LLPS-Scm-1532	Scophthalmus maximus	93.33	4e-152	430
LLPS-Gaa-0211	Gasterosteus aculeatus	90.79	7e-150	424
LLPS-Cii-2037	Ciona intestinalis	77.59	1e-125	363
LLPS-Drm-0430	Drosophila melanogaster	77.49	4e-127	366
LLPS-Cis-1485	Ciona savignyi	77.49	6e-125	361
LLPS-Cae-0967	Caenorhabditis elegans	71.5	7e-102	302
LLPS-Cus-0141	Cucumis sativus	62.11	2e-81	249
LLPS-Thc-1818	Theobroma cacao	62.11	6e-81	248
LLPS-Mae-1513	Manihot esculenta	62.11	3e-81	248
LLPS-Met-0744	Medicago truncatula	61.98	5e-82	250
LLPS-Vir-1069	Vigna radiata	61.46	2e-81	249
LLPS-Via-1234	Vigna angularis	61.46	2e-81	249
LLPS-Phv-1194	Phaseolus vulgaris	61.46	3e-81	248
LLPS-Php-1103	Physcomitrella patens	61.05	9e-79	243
LLPS-Pot-0342	Populus trichocarpa	61.05	1e-80	247
LLPS-Coc-1004	Corchorus capsularis	60.8	4e-72	225
LLPS-Amt-0537	Amborella trichopoda	60.51	9e-82	249
LLPS-Gor-0633	Gossypium raimondii	60.51	3e-81	248
LLPS-Nia-0663	Nicotiana attenuata	60.32	1e-79	244
LLPS-Sob-0679	Sorghum bicolor	60.22	5e-76	235
LLPS-Arl-0682	Arabidopsis lyrata	60.0	2e-82	251
LLPS-Mua-0603	Musa acuminata	60.0	3e-81	248
LLPS-Brn-1773	Brassica napus	60.0	3e-83	253
LLPS-Bro-0176	Brassica oleracea	60.0	3e-83	253
LLPS-Prp-0444	Prunus persica	60.0	1e-80	247
LLPS-Brr-0354	Brassica rapa	60.0	4e-83	253
LLPS-Sol-0830	Solanum lycopersicum	59.79	9e-79	242
LLPS-Sei-1167	Setaria italica	59.68	8e-76	234
LLPS-Ori-0481	Oryza indica	59.68	8e-76	234
LLPS-Zem-1010	Zea mays	59.68	8e-76	234
LLPS-Org-0067	Oryza glaberrima	59.68	8e-76	234
LLPS-Orbr-1140	Oryza brachyantha	59.68	8e-76	234
LLPS-Ors-1579	Oryza sativa	59.68	8e-76	234
LLPS-Hea-1186	Helianthus annuus	59.47	4e-78	240
LLPS-Tru-0792	Triticum urartu	59.17	1e-67	214
LLPS-Art-2272	Arabidopsis thaliana	58.97	7e-82	250
LLPS-Hov-0507	Hordeum vulgare	58.95	2e-77	238
LLPS-Lep-0635	Leersia perrieri	58.95	1e-77	239
LLPS-Tra-0936	Triticum aestivum	57.73	1e-77	239
LLPS-Viv-1507	Vitis vinifera	57.71	1e-79	246
LLPS-Brd-1388	Brachypodium distachyon	56.99	1e-71	226
LLPS-Chr-0959	Chlamydomonas reinhardtii	56.91	4e-72	226
LLPS-Asf-1189	Aspergillus flavus	56.06	6e-73	229
LLPS-Aso-0252	Aspergillus oryzae	56.06	6e-73	229
LLPS-Asni-0628	Aspergillus niger	56.06	2e-72	228
LLPS-Asfu-1180	Aspergillus fumigatus	56.06	1e-72	231
LLPS-Nef-0612	Neosartorya fischeri	56.06	6e-73	229
LLPS-Put-0330	Puccinia triticina	55.61	3e-75	233
LLPS-Asc-0960	Aspergillus clavatus	55.56	1e-72	229
LLPS-Mel-0575	Melampsora laricipopulina	55.39	2e-76	236
LLPS-Pug-0931	Puccinia graminis	55.39	3e-76	236
LLPS-Tum-0124	Tuber melanosporum	55.1	5e-71	224
LLPS-Blg-1157	Blumeria graminis	54.55	2e-70	223
LLPS-Spr-0836	Sporisorium reilianum	53.77	1e-72	226
LLPS-Usm-0481	Ustilago maydis	53.77	4e-72	225
LLPS-Cog-1241	Colletotrichum gloeosporioides	53.61	3e-71	225
LLPS-Pyt-0426	Pyrenophora teres	53.54	8e-70	221
LLPS-Coo-0291	Colletotrichum orbiculare	53.33	1e-70	223
LLPS-Map-0200	Magnaporthe poae	53.27	5e-71	224
LLPS-Mao-0865	Magnaporthe oryzae	53.27	1e-70	223
LLPS-Gag-1190	Gaeumannomyces graminis	53.27	8e-71	224
LLPS-Ved-1046	Verticillium dahliae	53.27	3e-70	223
LLPS-Cogr-0283	Colletotrichum graminicola	53.06	2e-71	225
LLPS-Lem-1541	Leptosphaeria maculans	53.03	7e-70	222
LLPS-Phn-0135	Phaeosphaeria nodorum	53.03	2e-69	220
LLPS-Osl-0891	Ostreococcus lucimarinus	52.08	1e-62	201
LLPS-Asn-0940	Aspergillus nidulans	51.01	2e-57	190
LLPS-Yal-0815	Yarrowia lipolytica	50.51	2e-60	197
LLPS-Orni-0904	Oryza nivara	50.27	2e-53	192
LLPS-Orb-1233	Oryza barthii	50.27	1e-53	192
LLPS-Orp-1306	Oryza punctata	50.0	5e-56	183
LLPS-Beb-1426	Beauveria bassiana	49.74	5e-65	209
LLPS-Pytr-0616	Pyrenophora triticirepentis	49.49	2e-58	192
LLPS-Miv-0983	Microbotryum violaceum	49.23	1e-58	196
LLPS-Dos-1080	Dothistroma septosporum	49.22	5e-62	201
LLPS-Ast-0646	Aspergillus terreus	48.13	5e-52	173
LLPS-Orgl-2272	Oryza glumaepatula	48.09	5e-30	124
LLPS-Orr-2039	Oryza rufipogon	48.09	5e-30	124
LLPS-Nec-0494	Neurospora crassa	47.9	6e-71	224
LLPS-Trv-1124	Trichoderma virens	47.23	3e-68	217
LLPS-Trr-1213	Trichoderma reesei	47.23	1e-68	218
LLPS-Fuv-0295	Fusarium verticillioides	47.03	6e-70	221
LLPS-Fuo-0046	Fusarium oxysporum	47.03	3e-70	223
LLPS-Fus-1072	Fusarium solani	47.01	7e-71	224
LLPS-Gas-0954	Galdieria sulphuraria	45.64	3e-53	177
LLPS-Zyt-0382	Zymoseptoria tritici	45.26	2e-63	205
LLPS-Dac-1156	Daucus carota	43.62	3e-48	164
LLPS-Glm-1337	Glycine max	43.45	4e-46	159
LLPS-Sot-1754	Solanum tuberosum	43.16	9e-52	172
LLPS-Chc-0829	Chondrus crispus	42.36	8e-43	154
LLPS-Crn-0134	Cryptococcus neoformans	42.23	1e-50	171
LLPS-Orm-1588	Oryza meridionalis	40.7	6e-39	140

LLPS-Mum-1531Nudt21

Protein Sequence

Nucleotide Sequence

LLPS-Mum-1531
Nudt21