LLPS-Mum-0269
Rif1

▼ OVERVIEW

Status: Reviewed
Protein Name: Telomere-associated protein RIF1; Rap1-interacting factor 1 homolog; mRif1
Gene Name: Rif1
Ensembl Gene: ENSMUSG00000036202.16
Ensembl Protein: ENSMUSP00000153998.1
Organism: Mus musculus
Taxa ID: 10090
LLPS Type: Others


▼ PROPERTY


▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs

▼ FUNCTION

Key regulator of TP53BP1 that plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage: acts by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs (PubMed:23333305, PubMed:23306437, PubMed:23306439). In response to DNA damage, interacts with ATM-phosphorylated TP53BP1 (PubMed:23333305, PubMed:23306437, PubMed:23306439). Interaction with TP53BP1 leads to dissociate the interaction between NUDT16L1/TIRR and TP53BP1, thereby unmasking the tandem Tudor-like domain of TP53BP1 and allowing recruitment to DNA DSBs (By similarity). Once recruited to DSBs, RIF1 and TP53BP1 act by promoting NHEJ-mediated repair of DSBs (PubMed:23333305, PubMed:23306437). In the same time, RIF1 and TP53BP1 specifically counteract the function of BRCA1 by blocking DSBs resection via homologous recombination (HR) during G1 phase (PubMed:23333305, PubMed:23306437). Also required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs (PubMed:23333305, PubMed:23333306, PubMed:23306439). Promotes NHEJ of dysfunctional telomeres (PubMed:23333305).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MTAPGRSPLE  PLLETWEDPS  VPPGEQTDAY  LTLTSRMTGE  EGKEVIAEIE  KNLSRLYTVL  60
61    KAHISSQNSE  LSSAALQALG  FCLYNPRITS  GLSEANIQEL  LLTLNGIIKS  SDKNVCTRAL  120
121   WVISKQTFPA  ELVSKMVSSI  IDSLEVILSK  GEIHSAVVDF  EALNVIISHW  FLHCRLIEQA  180
181   PVQMGEESVR  WAKLVIPLVV  HSAQKVHLRG  ATALEMGMPL  LLQKQQEIAL  ITEHLMTTKL  240
241   ISELQKLFKN  KNETYVLKLW  PLFVKLLGKT  LHRSGSFINS  LLQLEELGFR  SGTPMIKKIA  300
301   FIAWKSLIDN  FALNPDILCS  AKRLKLLMQP  LSSIHVRTET  LALTKLEVWW  YLLMRLGPQL  360
361   PANFEQVCVP  LIQSTISVDS  IPSPQGNSSR  GSASPGLSPL  TPGHKGASPY  GSPRGNLSSN  420
421   TGGMAAIPSI  QLLGLEMMLH  FLLGPEVLSF  AKQHKIVLSL  EPLEHPLISS  PSFFSKYAHT  480
481   LITAVHDSFV  SVGKDASDAV  VSAIWKELIS  LVKSVTEAGN  RKEKSGSEVL  TLLLKSLENI  540
541   VKSEVFPVSK  TLVLMEITVK  GLPPKVLGSP  AYQVANMDIL  NGTPALFLIQ  LIFNNNLLEC  600
601   GVEDEKFFLN  LETLVGCVLS  GPTSPLAFSD  SVLTVINQNA  KQLVNKEHLW  RMWSMIVSPL  660
661   TDVIHQTNEV  NQGDALEHNF  SAIYGALTLP  INHIFSAQTF  PTGTMKALLK  TWSELYRAFT  720
721   RCASLVATAE  ENLCCEELSS  KIMCSLEDEV  LSDLLFLDRI  SHIIIVMVDC  IDFSPYNKKY  780
781   QPKIKSPQRS  SDWSRKKKEP  LGKLASLFKL  IVKVIDTFHT  LSLKETFSDT  LLAIGNSIIS  840
841   MLSNVFGHIS  LPSMIREIFA  TFTRPLALLY  ENSKLDEAPK  VYTSLNNKLE  KLLGEIVACL  900
901   QFSYLGAYDS  ELLEHLSPLL  CVIFLHKNKQ  IRKQSALLWN  ATFAKATALV  YPEELKPILR  960
961   QAKQKILLLL  PGLENVEMMD  ESSEPYSEST  ENSQLNVKIS  GMERKSSGKR  DSILAHTKDK  1020
1021  KKKVKLSAKL  KLESSSPKIK  SGKLLEEEKS  TDFVFIPPEG  KETKARVLTE  HQKEVLKTKR  1080
1081  CDIPALYNNL  DASQDTLFSA  QFSQEESMES  LTLTEKPKED  AKIIKEEQME  STIFIHQDAP  1140
1141  ENCGIDEHSE  NASLPNCGGS  VAETNPETLI  TGFDARKEVL  ISSKILSAES  SSSTETSVVS  1200
1201  SSSVSNATFS  GTPPQPTSRR  QTFITLEKFD  GSETRPFSPS  PLNNISSTVT  VRNNQDNTTN  1260
1261  TDMPPKARKR  EVTNSKSDSE  NLANAGKKSS  RRWSKAEQSV  TKKSKPSLTS  EQEEHSSENN  1320
1321  SPDLLSPTEH  VSENDDHPSE  ATLEHKDGDP  KPAVENASLE  DLTTEEKNVG  INMESKESTA  1380
1381  SVVARTEQIV  NEDSQAAALA  PNPKTLRRSS  RRRSEAVDSC  SDSQERESGQ  QKKERRKEEE  1440
1441  KIISKSPLRI  KDDKLPTQKL  TDESPIQENL  TEKGNTLPER  TSGEPSVNAE  IDQNRRKPDL  1500
1501  ENVSSEGGGG  TLDNLDKSSE  KPLRGRTRYQ  TRRASQGLIS  AVENSESDSS  EAKEEVSRKK  1560
1561  RSGKWKNRSS  DSVDIEEQEE  KKAEEEVMKT  ANQTLDGQAV  PDVDVNAAAQ  VCEKSTNNNR  1620
1621  VILQDSAGPA  DSLQAPPKGE  EKSKINKCVD  SSFVSLPVPE  SNLRTRNASK  RLLYKQDNDS  1680
1681  NVRVSDSSLS  PEKFTQVECQ  HKRSRRVRRS  KSCDCCGEKS  QSQEKSFIGL  KNTESYAIKS  1740
1741  VEKKKTDLQV  PETAPETREA  RDHAETKLAG  EEPLVNFHVG  LKEENCTTGD  SVKSEAELQE  1800
1801  ASLPPEIVTV  KEKTYDTDAS  EAVSEIQGPC  SENHSPAEDP  GLSECKDISQ  KQLSENGELD  1860
1861  ISDVGKACKV  IAGSSPEGVE  TMELNVRNDA  FVAADSEKST  QMDVSVDVAT  EEDNKKDECE  1920
1921  AVTTEVNVEG  VATEDFNSGM  DLSDTPIPVS  KDVETEHAAS  GEIEGESNES  DSGSCEEMNK  1980
1981  EMGSHKAQMS  TEIDSARVKE  TDILASASKS  EEALIGRLDV  NTQSFVSDIE  MSSGERTVNC  2040
2041  KTETSIELNK  LDEAKLSGNE  ATVGNDTLQE  VCFTSEKVEK  LPQCLLVQVA  SELGAESNTT  2100
2101  SPEKLELDSF  GSVNESPSGM  QQARCVWSPL  ASPSTSILKR  GLKRSQEDEI  SPVNKIRRVS  2160
2161  FADPIYQAGL  ADDIDRRCSV  VRSHSSNSSP  IIKSVKTSPT  SHSKHNTTSA  KGFLSPGSQS  2220
2221  SKFKSPKKCL  ITEMAQESML  SPTESVYPAL  VNCAASVDII  LPQITSNMWA  RGLGQLIRAK  2280
2281  NIKTIGDLST  LTASEIKTLP  IRSPKVFNVK  KALRVYHEQQ  MKSRGLEEIP  IFDISEKAVN  2340
2341  GVESRTVSTD  EERFASDLIE  PVTLDTPLSK  NLVAQISALA  LQLDSEDLYS  YTGSQLFEMH  2400
2401  EKLGTMANSI  IRNLQSRWRS  PAHENS  2426
Nucleotide CDS Sequence (FASTA)
1     NNGGAACCCA  GTGTTAATGC  TGAAATTGAC  CAAAATAGAA  GAAAACCAGA  CCTTGAGAAT  60
61    GTTAGTTCTG  AAGGAGGTGG  TGGTACCCTG  GACAATCTAG  ACAAGTCGTC  TGAGAAACCT  120
121   TTAAGAGGAC  GGACACGTTA  TCAAACAAGA  AGAGCTTCGC  AGGGTTTGAT  TTCTGCTGTT  180
181   GAAAACTCAG  AATCTGACAG  TTCTGAGGCA  AAGGAAGAAG  TTTCTAGAAA  GAAACGATCA  240
241   GGGAAATGGA  AAAATAGAAG  CAGTGACAGT  GTTGACATTG  AAGAACAAGA  AGAAAAAAAG  300
301   GCTGAAGAGG  AAGTTATGAA  AACTGCAAAT  CAGACACTCG  ATGGCCAGGC  AGTTCCTGAT  360
361   GTTGATGTAA  ATGCAGCGGC  TCAGGTTTGT  GAAAAAAGTA  CAAATAACAA  CAGGGTCATC  420
421   CTCCAGGATT  CTGCTGGGCC  TGCAGATTCA  CTGCAAGCTC  CACCCAAAGG  CGAGGAGAAA  480
481   AGTAAGATTA  ACAAATGTGT  AGACAGTTCA  TTTGTAAGTC  TACCTGTGCC  AGAGTCAAAC  540
541   CTCAGGACTA  GGAATGCCAG  TAAGAGATTA  TTATATAAAC  AAGATAATGA  TAGTAATGTG  600
601   AGGGTATCAG  ACAGCTCTCT  GTCTCCCGAA  AAATTCACCC  AAGTTGAATG  CCAACACAAG  660
661   AGAAGTAGGA  GAGTCAGGAG  ATCTAAAAGT  TGTGACTGCT  GTGGCGAAAA  ATCACAGTCC  720
721   CAGGAAAAGT  CATTTATTGG  GTTAAAGAAC  ACAGAAAGTT  ATGCTATAAA  GAGTGTGGAG  780
781   AAAAAAAAGA  CAGATCTACA  AGTACCTGAG  ACTGCCCCTG  AAACTCGTGA  AGCTCGTGAC  840
841   CATGCTGAAA  CAAAGTTGGC  AGGCGAAGAG  CCTCTTGTGA  ATTTTCATGT  GGGTCTTAAA  900
901   GAAGAGAATT  GTACTACTGG  TGATTCAGTT  AAGTCTGAGG  CTGAGTTGCA  AGAAGCTTCC  960
961   CTTCCACCTG  AAATAGTAAC  TGTGAAAGAG  AAGACTTACG  ATACAGACGC  TAGTGAAGCA  1020
1021  GTGTCTGAAA  TCCAAGGGCC  ATGTAGTGAG  AACCACAGCC  CTGCTGAGGA  CCCAGGCTTA  1080
1081  AGTGAGTGCA  AAGACATTTC  ACAGAAGCAG  CTTTCAGAGA  ACGGAGAGCT  TGACATCAGC  1140
1141  GATGTAGGGA  AGGCATGCAA  AGTCATAGCT  GGGTCCAGTC  CAGAGGGAGT  TGAAACTATG  1200
1201  GAATTAAATG  TAAGAAATGA  TGCATTTGTA  GCTGCTGACT  CTGAAAAGAG  TACCCAAATG  1260
1261  GATGTTTCTG  TAGATGTGGC  AACAGAGGAA  GATAATAAAA  AAGATGAATG  TGAAGCAGTC  1320
1321  ACAACTGAAG  TGAATGTTGA  AGGAGTAGCC  ACTGAGGACT  TTAATTCAGG  TATGGATCTT  1380
1381  TCTGATACTC  CCATACCTGT  AAGCAAGGAT  GTTGAGACTG  AGCACGCAGC  TAGTGGGGAA  1440
1441  ATAGAGGGGG  AGAGCAATGA  ATCTGATTCA  GGCTCATGTG  AGGAAATGAA  CAAAGAAATG  1500
1501  GGAAGTCACA  AAGCCCAGAT  GAGCACTGAA  ATTGACAGTG  CCAGGGTAAA  GGAGACGGAC  1560
1561  ATACTTGCCA  GTGCCTCTAA  ATCTGAAGAA  GCGCTGATTG  GAAGACTAGA  CGTCAACACC  1620
1621  CAGAGCTTTG  TTAGTGACAT  TGAAATGAGT  TCTGGAGAAA  GAACTGTTAA  TTGCAAAACT  1680
1681  GAGACATCCA  TTGAACTTAA  TAAATTAGAT  GAAGCAAAAT  TAAGTGGCAA  TGAAGCTACA  1740
1741  GTGGGAAATG  ATACTCTGCA  GGAAGTTTGC  TTTACTTCAG  AGAAAGTGGA  GAAATTACCA  1800
1801  CAGTGTCTGC  TAGTTCAAGT  GGCTTCTGAA  CTTGGAGCAG  AAAGCAATAC  TACGTCTCCC  1860
1861  GAAAAGTTAG  AGCTGGATTC  TTTTGGATCA  GTAAATGAAA  GTCCTAGTGG  TATGCAGCAG  1920
1921  GCACGTTGTG  TCTGGTCACC  TTTGGCTTCT  CCATCCACAA  GCATTTTAAA  GAGAGGACTA  1980
1981  AAAAGATCCC  AAGAAGATGA  GATCTCACCA  GTTAACAAGA  TTAGACGTGT  CTCGTTTGCA  2040
2041  GATCCAATAT  ACCAAGCAGG  ATTGGCAGAT  GACATTGATA  GGCGTTGCTC  TGTTGTTAGA  2100
2101  TCTCATTCTT  CAAATAGCTC  TCCCATAATA  AAAAGTGTTA  AAACTTCACC  TACTTCACAC  2160
2161  TCTAAGAAAT  GGCCCAAGAA  TCCATGCTGT  CCCCAACAGA  AAGTGTTTAC  CCAGCTTTGG  2220
2221  TGA  2223

▼ KEYWORD

ID
Family
Alternative splicing
Cell cycle
Chromosome
Complete proteome
Cytoplasm
Cytoskeleton
DNA damage
DNA repair
Nucleus
Phosphoprotein
Reference proteome
Telomere

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Chromosome, telomeric region
Cellular Component
Condensed chromosome
Cellular Component
Cytoplasm
Cellular Component
Female pronucleus
Cellular Component
Male pronucleus
Cellular Component
Nuclear body
Cellular Component
Nuclear chromatin
Cellular Component
Nuclear membrane
Cellular Component
Nucleus
Cellular Component
Plasma membrane
Cellular Component
Site of double-strand break
Cellular Component
Spindle midzone
Biological Process
Cell cycle
Biological Process
Cellular response to DNA damage stimulus
Biological Process
Cellular response to leukemia inhibitory factor
Biological Process
Chromatin silencing at telomere
Biological Process
DNA repair
Biological Process
Negative regulation of double-strand break repair via homologous recombination
Biological Process
Negative regulation of transcription by RNA polymerase II
Biological Process
Positive regulation of double-strand break repair via nonhomologous end joining
Biological Process
Positive regulation of histone H3-K9 methylation
Biological Process
Positive regulation of isotype switching
Biological Process
Stem cell population maintenance
Biological Process
Telomere maintenance
Biological Process
Telomere maintenance in response to DNA damage

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Mea-1405Mesocricetus auratus94.260.0 711
LLPS-Ran-0837Rattus norvegicus86.180.03756
LLPS-Gog-3553Gorilla gorilla84.820.01175
LLPS-Cas-3201Carlito syrichta81.722e-35 153
LLPS-Ora-1220Ornithorhynchus anatinus72.780.01444
LLPS-Ict-1541Ictidomys tridecemlineatus71.180.03013
LLPS-Mal-0156Mandrillus leucophaeus69.690.02984
LLPS-Cea-1384Cercocebus atys69.610.02986
LLPS-Aim-1107Ailuropoda melanoleuca69.60.02998
LLPS-Poa-3570Pongo abelii69.590.02992
LLPS-Man-4606Macaca nemestrina69.570.02984
LLPS-Chs-2799Chlorocebus sabaeus69.560.02976
LLPS-Pat-1753Pan troglodytes69.560.02993
LLPS-Tut-2385Tursiops truncatus69.550.02960
LLPS-Hos-2931Homo sapiens69.550.02999
LLPS-Maf-0046Macaca fascicularis69.530.02979
LLPS-Mam-3964Macaca mulatta69.530.02982
LLPS-Pap-2149Pan paniscus69.440.02984
LLPS-Paa-4625Papio anubis69.410.02977
LLPS-Sus-1776Sus scrofa69.180.02928
LLPS-Ova-4260Ovis aries69.080.02894
LLPS-Fud-4286Fukomys damarensis69.030.02931
LLPS-Aon-4576Aotus nancymaae68.850.02961
LLPS-Bot-4636Bos taurus68.760.02673
LLPS-Mup-0713Mustela putorius furo68.70.02919
LLPS-Eqc-4642Equus caballus68.60.02977
LLPS-Rhb-2662Rhinopithecus bieti68.450.02929
LLPS-Fec-4098Felis catus68.240.02917
LLPS-Caj-2999Callithrix jacchus68.170.02924
LLPS-Otg-3878Otolemur garnettii68.160.02931
LLPS-Loa-1212Loxodonta africana68.140.02909
LLPS-Sah-2934Sarcophilus harrisii68.072e-134 476
LLPS-Nol-2463Nomascus leucogenys67.880.02874
LLPS-Caf-4016Canis familiaris67.670.02877
LLPS-Orc-4067Oryctolagus cuniculus67.630.02865
LLPS-Cap-1374Cavia porcellus66.880.02770
LLPS-Myl-0500Myotis lucifugus66.520.02489
LLPS-Pes-1492Pelodiscus sinensis66.240.01258
LLPS-Tag-3159Taeniopygia guttata64.30.01323
LLPS-Anp-2838Anas platyrhynchos62.822e-112 405
LLPS-Lac-3974Latimeria chalumnae62.321e-103 377
LLPS-Fia-0172Ficedula albicollis62.052e-101 369
LLPS-Meg-2802Meleagris gallopavo61.195e-103 375
LLPS-Gaga-1061Gallus gallus57.761e-107 389
LLPS-Anc-2453Anolis carolinensis56.128e-88 325
LLPS-Mod-1861Monodelphis domestica55.40.02223
LLPS-Xet-2057Xenopus tropicalis55.10.01144
LLPS-Scf-1619Scleropages formosus54.690.01137
LLPS-Dar-1122Danio rerio54.040.01119
LLPS-Gaa-2824Gasterosteus aculeatus53.283e-48 195
LLPS-Icp-2559Ictalurus punctatus51.840.01133
LLPS-Leo-0037Lepisosteus oculatus51.580.01134
LLPS-Asm-0751Astyanax mexicanus50.870.01130
LLPS-Pof-4072Poecilia formosa50.70.0 958
LLPS-Orn-1169Oreochromis niloticus49.920.01132
LLPS-Ten-1311Tetraodon nigroviridis48.54e-65 251
LLPS-Scm-1747Scophthalmus maximus48.470.01082
LLPS-Tar-3252Takifugu rubripes47.360.01047
LLPS-Xim-3480Xiphophorus maculatus46.890.01009
LLPS-Drm-0724Drosophila melanogaster42.596e-1172.8
LLPS-Orl-3810Oryzias latipes41.541e-51 206
LLPS-Sem-0861Selaginella moellendorffii27.357e-0759.3
LLPS-Zem-2146Zea mays27.132e-0967.8
LLPS-Mae-0666Manihot esculenta25.636e-0966.2
LLPS-Hov-1645Hordeum vulgare25.316e-1482.4
LLPS-Tra-2952Triticum aestivum25.01e-1381.3
LLPS-Orbr-0007Oryza brachyantha25.03e-0967.0
LLPS-Orp-0014Oryza punctata24.863e-1173.6
LLPS-Dac-2407Daucus carota24.623e-1483.6
LLPS-Brd-1703Brachypodium distachyon24.073e-1173.6
LLPS-Hea-0242Helianthus annuus24.05e-1585.9
LLPS-Orgl-1269Oryza glumaepatula23.981e-1071.2
LLPS-Orb-1844Oryza barthii23.982e-1070.9
LLPS-Org-1047Oryza glaberrima23.982e-1070.9
LLPS-Lep-2044Leersia perrieri23.958e-1172.4
LLPS-Orm-0509Oryza meridionalis23.895e-1069.3
LLPS-Prp-0742Prunus persica23.881e-1072.0
LLPS-Nia-0559Nicotiana attenuata23.879e-0965.5
LLPS-Sei-0751Setaria italica23.817e-1069.3
LLPS-Orni-1479Oryza nivara23.685e-1069.7
LLPS-Ori-2133Oryza indica23.683e-1070.5
LLPS-Orr-1854Oryza rufipogon23.683e-1070.5
LLPS-Ors-2146Oryza sativa23.683e-1070.5
LLPS-Gor-1766Gossypium raimondii23.515e-1276.3
LLPS-Sot-2164Solanum tuberosum23.266e-1172.8
LLPS-Lem-0761Leptosphaeria maculans23.263e-0863.9
LLPS-Coc-0235Corchorus capsularis23.043e-1277.0
LLPS-Met-2021Medicago truncatula22.993e-1070.5
LLPS-Sol-2509Solanum lycopersicum22.971e-1175.1
LLPS-Glm-2416Glycine max22.773e-1070.1
LLPS-Phv-2225Phaseolus vulgaris22.743e-1070.5
LLPS-Pot-2185Populus trichocarpa22.459e-1172.0
LLPS-Via-2354Vigna angularis22.296e-0965.9
LLPS-Viv-2494Vitis vinifera22.281e-1071.2
LLPS-Thc-1740Theobroma cacao21.38e-1068.9