LLPS-Mum-0182
Emg1
▼ OVERVIEW
| Status: | Reviewed |
| Protein Name: | Ribosomal RNA small subunit methyltransferase NEP1; 18S rRNA (pseudouridine(1248)-N1)-methyltransferase; 18S rRNA Psi1248 methyltransferase; Nucleolar protein EMG1 homolog; Protein C2f; Ribosome biogenesis protein NEP1 |
| Gene Name: | Emg1, C2f, Grcc2f |
| Ensembl Gene: | ENSMUSG00000004268.11 |
| Ensembl Protein: | ENSMUSP00000004379.5 |
| Organism: | Mus musculus |
| Taxa ID: | 10090 |
| LLPS Type: | Client |
▼ Classification
Condensates:
| Condensate | Description | Tissue/Cell | PMIDs |
|---|
▼ FUNCTION
| S-adenosyl-L-methionine-dependent pseudouridine N(1)-methyltransferase that methylates pseudouridine at position 1248 (Psi1248) in 18S rRNA. Involved the biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi) conserved in eukaryotic 18S rRNA. Is not able to methylate uridine at this position. Has also an essential role in 40S ribosomal subunit biogenesis independent on its methyltransferase activity, facilitating the incorporation of ribosomal protein S19 during the formation of pre-ribosomes. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | ENSMUST00000004379.7 | ENSMUSP00000004379.5 |
| Ensembl | ENSMUST00000203238.1 | ENSMUSP00000144730.1 |
| UniProt | O35130, NEP1_MOUSE | |
| GeneBank | BC002004, AC002397 | AAC36006.1, AAH02004.1 |
| RefSeq | NM_013536.2 | NP_038564.1 |
| Entrez | 14791 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MSAASGGFQP RERRFSVQEQ DWETTPPKKL RLGAGSKCGG RRLIVVLEGA SLETVKVGKT 60 61 YELLNCDRHK SMLLKNGRDP GEVRPDITHQ SLLMLMDSPL NRAGLLQVYI HTQKNVLIEV 120 121 NPQTRIPRTF DRFCGLMVQL LHKLSVRAAD GPQKLLKVIK NPVSDHFPVG CMKIGTSFSV 180 181 EDISDIRELV PSSDPVVFVV GAFAHGKVSV EYTEKMVSIS NYPLSAALTC AKVTTAFEEV 240 241 WGVI 244 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGTCTGCGG CCAGTGGTGG CTTCCAACCT CGTGAGCGGC GATTTTCAGT GCAGGAGCAG 60 61 GACTGGGAGA CTACGCCGCC TAAGAAGCTC CGGCTTGGGG CAGGAAGCAA GTGCGGAGGC 120 121 CGGAGGCTCA TTGTGGTGCT GGAAGGGGCC AGTCTGGAGA CAGTCAAGGT AGGGAAAACT 180 181 TACGAGCTAC TCAACTGTGA CAGGCACAAG TCCATGTTGT TGAAGAATGG ACGGGACCCA 240 241 GGGGAAGTCA GACCAGACAT CACCCACCAG AGCCTGCTGA TGCTTATGGA CAGCCCCCTG 300 301 AACCGAGCTG GCTTGCTACA GGTTTACATC CACACACAGA AGAACGTGCT GATTGAAGTG 360 361 AACCCCCAGA CTCGAATTCC TAGAACCTTT GACCGATTTT GTGGCCTCAT GGTTCAGCTT 420 421 TTACACAAAC TGAGCGTCCG AGCAGCCGAC GGCCCTCAGA AGCTATTGAA GGTAATTAAG 480 481 AATCCAGTGT CCGACCACTT CCCAGTTGGC TGTATGAAAA TTGGCACTTC CTTTTCTGTT 540 541 GAAGACATCA GTGACATTCG AGAGTTGGTG CCCAGTAGTG ACCCAGTTGT GTTTGTGGTG 600 601 GGGGCCTTTG CCCATGGCAA GGTCAGTGTG GAGTACACAG AAAAGATGGT GTCCATCAGC 660 661 AACTATCCAC TCTCTGCTGC GCTTACCTGT GCTAAAGTCA CCACAGCTTT TGAAGAAGTA 720 721 TGGGGTGTCA TTTGA 735 |
▼ KEYWORD
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Nucleolus | |
Cellular Component | Nucleus | |
Cellular Component | Small-subunit processome | |
Molecular Function | RRNA (pseudouridine) methyltransferase activity | |
Molecular Function | RRNA binding | |
Biological Process | Blastocyst development | |
Biological Process | Nucleologenesis | |
Biological Process | RRNA base methylation |
▼ ANNOTATION
| Mutation | ||||
| CGAP | ||||
| Physicochemical | ||||
| Compute pI/Mw | AAindex | |||
| Function | ||||
| CellMarker | ||||
| Localization | ||||
| COMPARTMENTS | ||||
| Expression | ||||
| ArrayExpress | GXD | TISSUES | ||
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Ran-3484 | Rattus norvegicus | 96.72 | 9e-176 | 491 |
| LLPS-Cas-1192 | Carlito syrichta | 93.38 | 5e-102 | 300 |
| LLPS-Mea-1565 | Mesocricetus auratus | 91.8 | 3e-169 | 474 |
| LLPS-Eqc-0075 | Equus caballus | 90.57 | 6e-163 | 458 |
| LLPS-Sus-2698 | Sus scrofa | 90.16 | 8e-163 | 458 |
| LLPS-Bot-0226 | Bos taurus | 90.16 | 2e-161 | 458 |
| LLPS-Ict-1669 | Ictidomys tridecemlineatus | 90.16 | 2e-162 | 457 |
| LLPS-Otg-0008 | Otolemur garnettii | 89.75 | 5e-161 | 453 |
| LLPS-Pap-0406 | Pan paniscus | 89.75 | 2e-163 | 459 |
| LLPS-Pat-0924 | Pan troglodytes | 89.75 | 2e-163 | 459 |
| LLPS-Cap-2468 | Cavia porcellus | 89.75 | 8e-163 | 458 |
| LLPS-Poa-1664 | Pongo abelii | 89.75 | 2e-163 | 459 |
| LLPS-Chs-0512 | Chlorocebus sabaeus | 89.34 | 8e-163 | 458 |
| LLPS-Maf-0706 | Macaca fascicularis | 89.34 | 8e-163 | 458 |
| LLPS-Cea-0588 | Cercocebus atys | 89.34 | 8e-163 | 458 |
| LLPS-Ere-0341 | Erinaceus europaeus | 89.34 | 2e-162 | 457 |
| LLPS-Mal-0302 | Mandrillus leucophaeus | 89.34 | 3e-163 | 459 |
| LLPS-Rhb-2103 | Rhinopithecus bieti | 89.34 | 4e-162 | 456 |
| LLPS-Man-1775 | Macaca nemestrina | 89.34 | 8e-163 | 458 |
| LLPS-Mam-1990 | Macaca mulatta | 89.34 | 8e-163 | 458 |
| LLPS-Tag-2786 | Taeniopygia guttata | 89.32 | 7e-138 | 393 |
| LLPS-Myl-1737 | Myotis lucifugus | 89.02 | 2e-160 | 452 |
| LLPS-Gog-0933 | Gorilla gorilla | 88.93 | 1e-160 | 452 |
| LLPS-Paa-2875 | Papio anubis | 88.93 | 3e-162 | 456 |
| LLPS-Orc-0713 | Oryctolagus cuniculus | 88.93 | 4e-161 | 454 |
| LLPS-Hos-1280 | Homo sapiens | 88.93 | 1e-161 | 455 |
| LLPS-Loa-1751 | Loxodonta africana | 88.93 | 1e-159 | 449 |
| LLPS-Nol-1302 | Nomascus leucogenys | 88.93 | 2e-161 | 454 |
| LLPS-Caf-0480 | Canis familiaris | 88.93 | 2e-160 | 452 |
| LLPS-Fia-1773 | Ficedula albicollis | 88.83 | 9e-137 | 392 |
| LLPS-Ova-0455 | Ovis aries | 88.62 | 7e-161 | 453 |
| LLPS-Aim-1170 | Ailuropoda melanoleuca | 88.52 | 7e-161 | 453 |
| LLPS-Urm-0909 | Ursus maritimus | 88.52 | 4e-160 | 451 |
| LLPS-Caj-0387 | Callithrix jacchus | 88.11 | 6e-160 | 451 |
| LLPS-Fud-1758 | Fukomys damarensis | 88.11 | 7e-160 | 451 |
| LLPS-Mup-0564 | Mustela putorius furo | 88.11 | 9e-160 | 450 |
| LLPS-Fec-1898 | Felis catus | 88.11 | 1e-160 | 452 |
| LLPS-Aon-0322 | Aotus nancymaae | 87.35 | 1e-157 | 444 |
| LLPS-Meg-0707 | Meleagris gallopavo | 86.89 | 1e-134 | 386 |
| LLPS-Pes-2442 | Pelodiscus sinensis | 86.76 | 4e-134 | 385 |
| LLPS-Sah-0923 | Sarcophilus harrisii | 85.25 | 4e-150 | 426 |
| LLPS-Anp-0258 | Anas platyrhynchos | 85.19 | 3e-122 | 353 |
| LLPS-Mod-1265 | Monodelphis domestica | 84.84 | 3e-150 | 426 |
| LLPS-Anc-2731 | Anolis carolinensis | 84.62 | 9e-134 | 384 |
| LLPS-Xet-0346 | Xenopus tropicalis | 81.28 | 3e-122 | 353 |
| LLPS-Gaga-0485 | Gallus gallus | 78.72 | 9e-137 | 391 |
| LLPS-Xim-1188 | Xiphophorus maculatus | 77.45 | 3e-116 | 339 |
| LLPS-Dar-1051 | Danio rerio | 75.98 | 8e-115 | 336 |
| LLPS-Dio-0403 | Dipodomys ordii | 74.18 | 6e-126 | 364 |
| LLPS-Scm-1464 | Scophthalmus maximus | 73.66 | 1e-116 | 340 |
| LLPS-Tar-1747 | Takifugu rubripes | 73.21 | 2e-117 | 343 |
| LLPS-Orl-0153 | Oryzias latipes | 72.77 | 5e-116 | 339 |
| LLPS-Pof-3225 | Poecilia formosa | 71.43 | 1e-116 | 340 |
| LLPS-Ten-0726 | Tetraodon nigroviridis | 70.76 | 2e-118 | 345 |
| LLPS-Icp-0214 | Ictalurus punctatus | 70.21 | 2e-89 | 272 |
| LLPS-Gaa-0497 | Gasterosteus aculeatus | 69.62 | 4e-115 | 337 |
| LLPS-Leo-2054 | Lepisosteus oculatus | 69.14 | 1e-119 | 348 |
| LLPS-Asm-2238 | Astyanax mexicanus | 69.07 | 5e-115 | 336 |
| LLPS-Orn-0119 | Oreochromis niloticus | 68.85 | 3e-117 | 342 |
| LLPS-Cii-0056 | Ciona intestinalis | 67.65 | 3e-112 | 329 |
| LLPS-Cis-0318 | Ciona savignyi | 67.24 | 2e-111 | 327 |
| LLPS-Scf-2225 | Scleropages formosus | 65.71 | 3e-111 | 327 |
| LLPS-Drm-1048 | Drosophila melanogaster | 59.58 | 6e-97 | 291 |
| LLPS-Glm-0468 | Glycine max | 54.85 | 1e-71 | 227 |
| LLPS-Abg-0549 | Absidia glauca | 54.63 | 2e-77 | 241 |
| LLPS-Cae-0983 | Caenorhabditis elegans | 54.59 | 2e-84 | 258 |
| LLPS-Sem-1157 | Selaginella moellendorffii | 54.37 | 2e-77 | 240 |
| LLPS-Vir-0003 | Vigna radiata | 54.37 | 1e-70 | 224 |
| LLPS-Via-0763 | Vigna angularis | 54.37 | 1e-70 | 224 |
| LLPS-Phv-1904 | Phaseolus vulgaris | 54.37 | 6e-72 | 228 |
| LLPS-Scj-0601 | Schizosaccharomyces japonicus | 54.25 | 6e-69 | 221 |
| LLPS-Usm-0965 | Ustilago maydis | 53.54 | 8e-71 | 228 |
| LLPS-Spr-0546 | Sporisorium reilianum | 53.51 | 1e-70 | 228 |
| LLPS-Scp-0802 | Schizosaccharomyces pombe | 52.83 | 3e-67 | 218 |
| LLPS-Php-0487 | Physcomitrella patens | 52.71 | 8e-77 | 239 |
| LLPS-Prp-0674 | Prunus persica | 52.68 | 1e-68 | 219 |
| LLPS-Ori-0227 | Oryza indica | 52.61 | 5e-66 | 213 |
| LLPS-Ors-0459 | Oryza sativa | 52.61 | 5e-66 | 213 |
| LLPS-Org-0686 | Oryza glaberrima | 52.61 | 9e-66 | 212 |
| LLPS-Met-0612 | Medicago truncatula | 52.43 | 1e-70 | 224 |
| LLPS-Amt-0491 | Amborella trichopoda | 52.43 | 2e-72 | 229 |
| LLPS-Scc-0066 | Schizosaccharomyces cryophilus | 52.36 | 3e-67 | 218 |
| LLPS-Sac-1198 | Saccharomyces cerevisiae | 52.34 | 1e-69 | 221 |
| LLPS-Yal-1165 | Yarrowia lipolytica | 52.13 | 2e-67 | 215 |
| LLPS-Sob-0184 | Sorghum bicolor | 52.13 | 1e-67 | 217 |
| LLPS-Zem-0242 | Zea mays | 52.13 | 2e-67 | 216 |
| LLPS-Crn-0942 | Cryptococcus neoformans | 52.05 | 8e-70 | 224 |
| LLPS-Tra-0318 | Triticum aestivum | 51.96 | 7e-66 | 212 |
| LLPS-Hov-0512 | Hordeum vulgare | 51.96 | 1e-65 | 211 |
| LLPS-Tru-0694 | Triticum urartu | 51.96 | 7e-66 | 212 |
| LLPS-Hea-0205 | Helianthus annuus | 51.94 | 1e-67 | 217 |
| LLPS-Brn-0185 | Brassica napus | 51.94 | 1e-69 | 222 |
| LLPS-Gor-0188 | Gossypium raimondii | 51.94 | 3e-70 | 224 |
| LLPS-Brr-0381 | Brassica rapa | 51.94 | 2e-69 | 222 |
| LLPS-Bro-0732 | Brassica oleracea | 51.94 | 1e-69 | 222 |
| LLPS-Lep-0377 | Leersia perrieri | 51.66 | 1e-66 | 214 |
| LLPS-Orbr-1268 | Oryza brachyantha | 51.66 | 4e-66 | 213 |
| LLPS-Viv-0584 | Vitis vinifera | 51.23 | 4e-63 | 205 |
| LLPS-Brd-1108 | Brachypodium distachyon | 51.18 | 1e-64 | 210 |
| LLPS-Osl-1196 | Ostreococcus lucimarinus | 51.13 | 2e-74 | 232 |
| LLPS-Chr-0527 | Chlamydomonas reinhardtii | 51.12 | 6e-76 | 236 |
| LLPS-Nia-0234 | Nicotiana attenuata | 50.97 | 5e-68 | 218 |
| LLPS-Asg-0375 | Ashbya gossypii | 50.93 | 1e-67 | 216 |
| LLPS-Tum-1207 | Tuber melanosporum | 50.93 | 6e-63 | 204 |
| LLPS-Mua-1374 | Musa acuminata | 50.73 | 1e-64 | 209 |
| LLPS-Miv-0521 | Microbotryum violaceum | 50.68 | 4e-62 | 204 |
| LLPS-Sei-1178 | Setaria italica | 50.54 | 5e-61 | 197 |
| LLPS-Sol-0564 | Solanum lycopersicum | 50.49 | 3e-68 | 218 |
| LLPS-Mae-1589 | Manihot esculenta | 50.25 | 3e-63 | 206 |
| LLPS-Arl-0162 | Arabidopsis lyrata | 50.24 | 4e-63 | 206 |
| LLPS-Asni-0095 | Aspergillus niger | 50.23 | 3e-64 | 207 |
| LLPS-Asn-0140 | Aspergillus nidulans | 50.0 | 4e-65 | 209 |
| LLPS-Dac-1853 | Daucus carota | 50.0 | 1e-66 | 214 |
| LLPS-Asf-0027 | Aspergillus flavus | 50.0 | 2e-64 | 207 |
| LLPS-Aso-0358 | Aspergillus oryzae | 50.0 | 2e-64 | 207 |
| LLPS-Zyt-0061 | Zymoseptoria tritici | 49.78 | 4e-66 | 212 |
| LLPS-Dos-0323 | Dothistroma septosporum | 49.77 | 4e-64 | 207 |
| LLPS-Ast-0195 | Aspergillus terreus | 49.54 | 7e-65 | 209 |
| LLPS-Art-0047 | Arabidopsis thaliana | 49.52 | 7e-66 | 213 |
| LLPS-Coc-0307 | Corchorus capsularis | 49.51 | 4e-66 | 213 |
| LLPS-Sot-1248 | Solanum tuberosum | 49.3 | 5e-69 | 220 |
| LLPS-Asc-0220 | Aspergillus clavatus | 49.08 | 7e-64 | 206 |
| LLPS-Nef-0113 | Neosartorya fischeri | 49.08 | 2e-64 | 208 |
| LLPS-Asfu-0517 | Aspergillus fumigatus | 49.08 | 2e-64 | 207 |
| LLPS-Thc-1116 | Theobroma cacao | 49.03 | 1e-64 | 209 |
| LLPS-Pug-0361 | Puccinia graminis | 48.67 | 1e-61 | 202 |
| LLPS-Fus-0873 | Fusarium solani | 48.65 | 5e-63 | 204 |
| LLPS-Fuv-0481 | Fusarium verticillioides | 48.62 | 1e-63 | 206 |
| LLPS-Fuo-0867 | Fusarium oxysporum | 48.62 | 1e-63 | 206 |
| LLPS-Orni-0803 | Oryza nivara | 48.34 | 1e-54 | 183 |
| LLPS-Orb-0208 | Oryza barthii | 48.34 | 9e-54 | 182 |
| LLPS-Orgl-0365 | Oryza glumaepatula | 48.34 | 7e-54 | 182 |
| LLPS-Orr-1695 | Oryza rufipogon | 48.34 | 1e-54 | 183 |
| LLPS-Mel-0646 | Melampsora laricipopulina | 48.18 | 1e-59 | 195 |
| LLPS-Kop-0689 | Komagataella pastoris | 48.15 | 5e-70 | 222 |
| LLPS-Nec-0512 | Neurospora crassa | 47.96 | 3e-62 | 202 |
| LLPS-Cus-1304 | Cucumis sativus | 47.8 | 2e-60 | 199 |
| LLPS-Pot-1413 | Populus trichocarpa | 47.66 | 2e-61 | 201 |
| LLPS-Scs-0351 | Sclerotinia sclerotiorum | 47.51 | 2e-62 | 202 |
| LLPS-Beb-0617 | Beauveria bassiana | 47.51 | 8e-63 | 204 |
| LLPS-Coo-0119 | Colletotrichum orbiculare | 47.49 | 3e-59 | 196 |
| LLPS-Orp-1062 | Oryza punctata | 47.39 | 4e-55 | 184 |
| LLPS-Trv-0122 | Trichoderma virens | 47.3 | 6e-61 | 199 |
| LLPS-Cogr-0512 | Colletotrichum graminicola | 47.27 | 3e-60 | 197 |
| LLPS-Cog-0574 | Colletotrichum gloeosporioides | 46.82 | 6e-60 | 196 |
| LLPS-Map-0227 | Magnaporthe poae | 46.4 | 1e-60 | 198 |
| LLPS-Lem-0133 | Leptosphaeria maculans | 46.36 | 9e-59 | 194 |
| LLPS-Chc-0343 | Chondrus crispus | 46.29 | 9e-62 | 204 |
| LLPS-Phn-0305 | Phaeosphaeria nodorum | 46.12 | 1e-58 | 193 |
| LLPS-Blg-0116 | Blumeria graminis | 46.05 | 2e-62 | 203 |
| LLPS-Pytr-0401 | Pyrenophora triticirepentis | 45.87 | 9e-57 | 187 |
| LLPS-Pyt-0277 | Pyrenophora teres | 45.21 | 7e-56 | 187 |
| LLPS-Gag-0984 | Gaeumannomyces graminis | 44.49 | 5e-60 | 197 |
| LLPS-Ved-0069 | Verticillium dahliae | 43.56 | 2e-60 | 197 |
| LLPS-Mao-1376 | Magnaporthe oryzae | 43.08 | 6e-62 | 202 |
| LLPS-Trr-0206 | Trichoderma reesei | 42.25 | 2e-58 | 192 |
| LLPS-Gas-0393 | Galdieria sulphuraria | 41.59 | 8e-60 | 196 |
| LLPS-Put-0054 | Puccinia triticina | 41.49 | 1e-33 | 130 |
| LLPS-Cym-0097 | Cyanidioschyzon merolae | 41.13 | 1e-58 | 192 |