LLPS-Hos-a206
CHMP4B

▼ OVERVIEW

Status: Reviewed
Protein Name: Charged multivesicular body protein 4b; Chromatin-modifying protein 4b; CHMP4b; SNF7 homolog associated with Alix 1; SNF7-2; hSnf7-2; Vacuolar protein sorting-associated protein 32-2; Vps32-2; hVps32-2
Gene Name: CHMP4B, C20orf178, SHAX1
Ensembl Gene: ENSG00000101421.3
Ensembl Protein: ENSP00000217402.2
Organism: Homo sapiens
Taxa ID: 9606
LLPS Type: Client
PDB: 5MK2 (A, C) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateDescriptionTissue/CellPMIDs
Postsynaptic density
"...We report the first direct comparison of the proteome of triplicate isolates of mouse and human cortical postsynaptic densities. The mouse postsynaptic density comprised 1556 proteins and the human one 1461."
Human cortex23071613

▼ FUNCTION

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released (PubMed:12860994, PubMed:18209100). The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:21310966). Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). Plays a role in the endosomal sorting pathway. ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. When overexpressed, membrane-assembled circular arrays of CHMP4B filaments can promote or stabilize negative curvature and outward budding. CHMP4A/B/C are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

▼ SEQUENCE

Protein Sequence (FASTA)
1     MSVFGKLFGA  GGGKAGKGGP  TPQEAIQRLR  DTEEMLSKKQ  EFLEKKIEQE  LTAAKKHGTK  60
61    NKRAALQALK  RKKRYEKQLA  QIDGTLSTIE  FQREALENAN  TNTEVLKNMG  YAAKAMKAAH  120
121   DNMDIDKVDE  LMQDIADQQE  LAEEISTAIS  KPVGFGEEFD  EDELMAELEE  LEQEELDKNL  180
181   LEISGPETVP  LPNVPSIALP  SKPAKKKEEE  DDDMKELENW  AGSM  224
Nucleotide CDS Sequence (FASTA)
1     ATGTCGGTGT  TCGGGAAGCT  GTTCGGGGCT  GGAGGGGGTA  AGGCCGGCAA  GGGCGGCCCG  60
61    ACCCCCCAGG  AGGCCATCCA  GCGGCTGCGG  GACACGGAAG  AGATGTTAAG  CAAGAAACAG  120
121   GAGTTCCTGG  AGAAGAAAAT  CGAGCAGGAG  CTGACGGCCG  CCAAGAAGCA  CGGCACCAAA  180
181   AACAAGCGCG  CGGCCCTCCA  GGCACTGAAG  CGTAAGAAGA  GGTATGAGAA  GCAGCTGGCG  240
241   CAGATCGACG  GCACATTATC  AACCATCGAG  TTCCAGCGGG  AGGCCCTGGA  GAATGCCAAC  300
301   ACCAACACCG  AGGTGCTCAA  GAACATGGGC  TATGCCGCCA  AGGCCATGAA  GGCGGCCCAT  360
361   GACAACATGG  ACATCGATAA  AGTTGATGAG  TTAATGCAGG  ACATTGCTGA  CCAGCAAGAA  420
421   CTTGCAGAGG  AGATTTCAAC  AGCAATTTCG  AAACCTGTAG  GGTTTGGAGA  AGAGTTTGAC  480
481   GAGGATGAGC  TCATGGCGGA  ATTAGAAGAA  CTAGAACAGG  AGGAACTAGA  CAAGAATTTG  540
541   CTGGAAATCA  GTGGACCCGA  AACAGTCCCT  CTACCAAATG  TTCCCTCTAT  AGCCCTACCA  600
601   TCAAAACCCG  CCAAGAAGAA  AGAAGAGGAG  GACGACGACA  TGAAGGAATT  GGAGAACTGG  660
661   GCTGGATCCA  TGTAA  675

▼ KEYWORD

ID
Family
3D-structure
Acetylation
Cataract
Coiled coil
Complete proteome
Cytoplasm
Direct protein sequencing
Disease mutation
Endosome
Membrane
Nucleus
Phosphoprotein
Protein transport
Reference proteome
Transport
Ubl conjugation

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cytoplasm
Cellular Component
Cytoplasmic side of plasma membrane
Cellular Component
Cytosol
Cellular Component
Endosome
Cellular Component
ESCRT III complex
Cellular Component
Extracellular exosome
Cellular Component
Late endosome membrane
Cellular Component
Membrane coat
Cellular Component
Midbody
Cellular Component
Nuclear envelope
Cellular Component
Nucleus
Cellular Component
Vesicle
Molecular Function
Cadherin binding
Molecular Function
Identical protein binding
Molecular Function
Protein homodimerization activity
Biological Process
Autophagy
Biological Process
Endosomal transport
Biological Process
Exit from mitosis
Biological Process
Macroautophagy
Biological Process
Maintenance of lens transparency
Biological Process
Membrane fission
Biological Process
Midbody abscission
Biological Process
Mitotic cytokinesis
Biological Process
Mitotic metaphase plate congression
Biological Process
Multivesicular body assembly
Biological Process
Negative regulation of autophagosome assembly
Biological Process
Negative regulation of cell death
Biological Process
Negative regulation of neuron death
Biological Process
Nuclear envelope reassembly
Biological Process
Nucleus organization
Biological Process
Positive regulation of viral release from host cell
Biological Process
Posttranslational protein targeting to endoplasmic reticulum membrane
Biological Process
Protein homooligomerization
Biological Process
Regulation of centrosome duplication
Biological Process
Regulation of mitotic spindle assembly
Biological Process
Regulation of viral process
Biological Process
Ubiquitin-independent protein catabolic process via the multivesicular body sorting pathway
Biological Process
Vacuolar transport
Biological Process
Viral budding
Biological Process
Viral budding via host ESCRT complex
Biological Process
Viral life cycle

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Pat-a199Pan troglodytes100.01e-158449
LLPS-Mal-a200Mandrillus leucophaeus100.08e-159448
LLPS-Cea-a203Cercocebus atys100.08e-159448
LLPS-Caj-a199Callithrix jacchus100.08e-159448
LLPS-Gog-a200Gorilla gorilla100.08e-159448
LLPS-Cas-a184Carlito syrichta100.03e-109321
LLPS-Aon-a206Aotus nancymaae100.08e-159448
LLPS-Chs-a202Chlorocebus sabaeus100.08e-159448
LLPS-Maf-a203Macaca fascicularis100.08e-159448
LLPS-Man-a201Macaca nemestrina100.04e-147418
LLPS-Paa-a202Papio anubis100.01e-158449
LLPS-Mam-a201Macaca mulatta100.08e-159448
LLPS-Nol-a206Nomascus leucogenys100.08e-159448
LLPS-Mum-a202Mus musculus99.551e-158447
LLPS-Orc-a197Oryctolagus cuniculus99.554e-158446
LLPS-Rhb-a198Rhinopithecus bieti99.554e-158446
LLPS-Otg-a201Otolemur garnettii99.112e-157444
LLPS-Ict-a202Ictidomys tridecemlineatus99.112e-157444
LLPS-Dio-a199Dipodomys ordii99.111e-157445
LLPS-Loa-a199Loxodonta africana98.663e-157444
LLPS-Ova-a200Ovis aries98.213e-156441
LLPS-Sus-a205Sus scrofa98.215e-156441
LLPS-Eqc-a206Equus caballus98.211e-156442
LLPS-Fud-a199Fukomys damarensis98.212e-156442
LLPS-Tut-a114Tursiops truncatus98.122e-107315
LLPS-Caf-a202Canis familiaris97.772e-155439
LLPS-Urm-a190Ursus maritimus97.666e-147417
LLPS-Fec-a202Felis catus97.328e-155438
LLPS-Ere-a043Erinaceus europaeus97.327e-154435
LLPS-Mup-a205Mustela putorius furo97.328e-155438
LLPS-Ran-a203Rattus norvegicus97.322e-154437
LLPS-Bot-a206Bos taurus97.325e-155438
LLPS-Aim-a197Ailuropoda melanoleuca96.621e-140401
LLPS-Cap-a201Cavia porcellus96.435e-154436
LLPS-Mea-a187Mesocricetus auratus96.437e-154436
LLPS-Sah-a195Sarcophilus harrisii96.273e-105310
LLPS-Pap-a199Pan paniscus95.952e-147419
LLPS-Anc-a190Anolis carolinensis95.653e-107317
LLPS-Mod-a193Monodelphis domestica94.672e-117343
LLPS-Poa-a203Pongo abelii93.756e-144410
LLPS-Myl-a194Myotis lucifugus92.446e-119347
LLPS-Pes-a184Pelodiscus sinensis91.981e-83254
LLPS-Fia-a186Ficedula albicollis91.367e-79243
LLPS-Anp-a183Anas platyrhynchos90.536e-86261
LLPS-Gaga-a190Gallus gallus88.842e-110325
LLPS-Ora-a181Ornithorhynchus anatinus87.293e-102303
LLPS-Icp-a185Ictalurus punctatus84.313e-102304
LLPS-Scf-a173Scleropages formosus83.195e-114334
LLPS-Asm-a174Astyanax mexicanus83.041e-103308
LLPS-Orl-a177Oryzias latipes82.673e-99296
LLPS-Dar-a179Danio rerio82.524e-95286
LLPS-Orn-a178Oreochromis niloticus81.251e-107318
LLPS-Pof-a180Poecilia formosa80.88e-101301
LLPS-Xim-a185Xiphophorus maculatus79.912e-99297
LLPS-Gaa-a178Gasterosteus aculeatus79.528e-101301
LLPS-Scm-a171Scophthalmus maximus77.832e-103307
LLPS-Tar-a174Takifugu rubripes77.52e-95287
LLPS-Ten-a176Tetraodon nigroviridis74.447e-102303
LLPS-Leo-a178Lepisosteus oculatus72.052e-79244
LLPS-Lac-a188Latimeria chalumnae65.579e-105311
LLPS-Xet-a180Xenopus tropicalis65.132e-82254
LLPS-Tag-a185Taeniopygia guttata63.758e-87265
LLPS-Meg-a177Meleagris gallopavo61.822e-78244
LLPS-Cae-a040Caenorhabditis elegans61.333e-79246
LLPS-Drm-a062Drosophila melanogaster57.144e-73230
LLPS-Abg-a020Absidia glauca55.981e-41149
LLPS-Cis-a059Ciona savignyi54.859e-51172
LLPS-Cii-a067Ciona intestinalis52.252e-56187