LLPS-Hos-4918
MLST8

▼ OVERVIEW

Status: Unreviewed
Protein Name: Target of rapamycin complex subunit LST8
Gene Name: MLST8, GBL, LST8
Ensembl Gene: ENSG00000167965.17
Ensembl Protein: ENSP00000459612.1
Organism: Homo sapiens
Taxa ID: 9606
LLPS Type: Others
PDB: 5FLC (1, 2, A, B, C, D) More


▼ PROPERTY


——— Disorder propensity (calculated by IUPred2A)

▼ Classification

Condensates:
CondensateEvidenceOrthologs
Stress granulePredicted from orthologs(View)

▼ FUNCTION

Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

▼ SEQUENCE

Protein Sequence (FASTA)
1     MNTSPGTVGS  DPVILATAGY  DHTVRFWQAH  SGICTRTVQH  QDSQVNALEV  TPDRSMIAAA  60
61    GYQHIRMYDL  NSNNPNPIIS  YDGVNKNIAS  VGFHEDGRWM  YTGGEDCTAR  IWDLRSRNLQ  120
121   CQRIFQVNAP  INCVCLHPNQ  AELIVGDQSG  AIHIWDLKTD  HNEQLIPEPE  VSITSAHIDP  180
181   DASYMAAVNS  TGNCYVWNLT  GGIGDEVTQL  IPKTKIPAHT  RYALQCRFSP  DSTLLATCSA  240
241   DQTCKIWRTS  NFSLMTELSI  KSGNPGESSR  GWMWGCAFSG  DSQYIVTGEP  RPGLPHPWPP  300
301   ALASRASPPR  LQLPLCWGRL  LGLHLRS  327
Nucleotide CDS Sequence (FASTA)
1     ATGAACACCT  CCCCAGGCAC  GGTGGGCAGT  GACCCGGTCA  TCCTGGCCAC  TGCAGGCTAC  60
61    GACCACACCG  TGCGCTTCTG  GCAGGCCCAC  AGCGGCATCT  GCACCCGGAC  GGTGCAGCAC  120
121   CAGGACTCCC  AGGTGAATGC  CTTGGAGGTC  ACACCGGACC  GCAGCATGAT  TGCTGCTGCA  180
181   GGTTACCAGC  ACATCCGCAT  GTATGATCTC  AACTCCAATA  ACCCTAACCC  CATCATCAGC  240
241   TACGACGGCG  TCAACAAGAA  CATCGCGTCT  GTGGGCTTCC  ACGAAGACGG  CCGCTGGATG  300
301   TACACGGGCG  GCGAGGACTG  CACAGCCAGG  ATCTGGGACC  TCAGGTCCCG  GAACCTGCAG  360
361   TGCCAGCGGA  TCTTCCAGGT  GAACGCACCC  ATTAACTGCG  TGTGCCTGCA  CCCCAACCAG  420
421   GCAGAGCTCA  TCGTGGGTGA  CCAGAGCGGG  GCTATCCACA  TCTGGGACTT  GAAAACAGAC  480
481   CACAACGAGC  AGCTGATCCC  TGAGCCCGAG  GTCTCCATCA  CGTCCGCCCA  CATCGATCCC  540
541   GACGCCAGCT  ACATGGCAGC  TGTCAATAGC  ACCGGAAACT  GCTATGTCTG  GAATCTGACG  600
601   GGGGGCATTG  GTGACGAGGT  GACCCAGCTC  ATCCCCAAGA  CTAAGATCCC  TGCCCACACG  660
661   CGCTACGCCC  TGCAGTGTCG  CTTCAGCCCC  GACTCCACGC  TCCTCGCCAC  CTGCTCGGCT  720
721   GATCAGACGT  GCAAGATCTG  GAGGACGTCC  AACTTCTCCC  TGATGACGGA  GCTGAGCATC  780
781   AAGAGCGGCA  ACCCCGGGGA  GTCCTCCCGC  GGCTGGATGT  GGGGCTGCGC  CTTCTCGGGG  840
841   GACTCCCAGT  ACATCGTCAC  TGGTGAGCCC  CGCCCTGGCC  TCCCCCATCC  CTGGCCCCCG  900
901   GCGCTGGCCT  CCAGAGCCAG  CCCACCTCGG  CTGCAGCTTC  CCCTCTGCTG  GGGCCGCCTG  960
961   CTTGGCCTGC  ACCTGCGCTC  TTAG  984

▼ KEYWORD

ID
Family
3D-structure
Acetylation
Alternative splicing
Complete proteome
Cytoplasm
Phosphoprotein
Reference proteome
Repeat
WD repeat

▼ GENE ONTOLOGY

ID
Classification
Description
Cellular Component
Cytoplasm
Cellular Component
Cytosol
Cellular Component
Nucleoplasm
Cellular Component
TORC1 complex
Cellular Component
TORC2 complex
Molecular Function
Protein serine/threonine kinase activator activity
Biological Process
Activation of protein kinase B activity
Biological Process
Cell cycle arrest
Biological Process
Positive regulation of TOR signaling
Biological Process
Regulation of actin cytoskeleton organization
Biological Process
Regulation of cellular response to heat
Biological Process
Regulation of macroautophagy
Biological Process
TOR signaling
Biological Process
TORC1 signaling

▼ KEGG


▼ ORTHOLOGY

DrLLPS IDOrganismIdentityE-valueScore
LLPS-Pat-3649Pan troglodytes99.690.0 651
LLPS-Pap-4115Pan paniscus99.690.0 651
LLPS-Gog-3873Gorilla gorilla99.310.0 598
LLPS-Caj-4191Callithrix jacchus99.310.0 598
LLPS-Poa-0103Pongo abelii99.310.0 598
LLPS-Fud-1874Fukomys damarensis98.960.0 596
LLPS-Chs-4527Chlorocebus sabaeus98.960.0 597
LLPS-Ict-2028Ictidomys tridecemlineatus98.960.0 596
LLPS-Cap-3311Cavia porcellus98.960.0 596
LLPS-Aon-3632Aotus nancymaae98.660.0 615
LLPS-Mup-3408Mustela putorius furo98.610.0 594
LLPS-Sus-3541Sus scrofa98.610.0 595
LLPS-Cas-1844Carlito syrichta98.610.0 595
LLPS-Caf-2576Canis familiaris98.610.0 595
LLPS-Orc-2646Oryctolagus cuniculus98.610.0 594
LLPS-Otg-2858Otolemur garnettii98.280.0 593
LLPS-Aim-3738Ailuropoda melanoleuca98.260.0 592
LLPS-Bot-3229Bos taurus98.260.0 595
LLPS-Mea-3891Mesocricetus auratus98.260.0 594
LLPS-Fec-2594Felis catus98.260.0 593
LLPS-Ova-1065Ovis aries97.920.0 590
LLPS-Dio-0804Dipodomys ordii97.920.0 591
LLPS-Urm-2424Ursus maritimus97.640.0 600
LLPS-Ran-3569Rattus norvegicus97.220.0 589
LLPS-Mum-4424Mus musculus97.220.0 588
LLPS-Mam-4324Macaca mulatta97.00.0 601
LLPS-Loa-4010Loxodonta africana96.880.0 586
LLPS-Mal-1773Mandrillus leucophaeus96.610.0 584
LLPS-Paa-4448Papio anubis96.610.0 583
LLPS-Maf-4634Macaca fascicularis96.610.0 583
LLPS-Cea-4229Cercocebus atys96.610.0 583
LLPS-Rhb-2631Rhinopithecus bieti96.610.0 583
LLPS-Man-3741Macaca nemestrina96.610.0 583
LLPS-Eqc-4378Equus caballus95.490.0 571
LLPS-Myl-0274Myotis lucifugus94.980.0 583
LLPS-Nol-0047Nomascus leucogenys94.950.0 621
LLPS-Sah-3743Sarcophilus harrisii94.440.0 574
LLPS-Mod-0323Monodelphis domestica94.440.0 574
LLPS-Anc-0974Anolis carolinensis93.40.0 570
LLPS-Pes-1915Pelodiscus sinensis93.137e-164 465
LLPS-Gaga-1468Gallus gallus93.060.0 572
LLPS-Fia-2642Ficedula albicollis93.060.0 572
LLPS-Lac-3291Latimeria chalumnae93.060.0 571
LLPS-Orn-1950Oreochromis niloticus92.360.0 566
LLPS-Scm-1094Scophthalmus maximus92.360.0 566
LLPS-Ora-2233Ornithorhynchus anatinus92.055e-98 295
LLPS-Orl-1403Oryzias latipes91.670.0 565
LLPS-Xim-2512Xiphophorus maculatus91.320.0 565
LLPS-Gaa-3868Gasterosteus aculeatus91.320.0 562
LLPS-Icp-2619Ictalurus punctatus91.320.0 564
LLPS-Leo-3070Lepisosteus oculatus91.320.0 561
LLPS-Dar-2282Danio rerio91.320.0 564
LLPS-Pof-0285Poecilia formosa91.320.0 565
LLPS-Meg-0326Meleagris gallopavo90.616e-162 461
LLPS-Tar-2080Takifugu rubripes90.280.0 560
LLPS-Ten-2666Tetraodon nigroviridis90.280.0 560
LLPS-Xet-3236Xenopus tropicalis89.930.0 551
LLPS-Anp-1937Anas platyrhynchos89.021e-159 455
LLPS-Scf-0324Scleropages formosus84.049e-176 497
LLPS-Tag-3289Taeniopygia guttata74.651e-144 418
LLPS-Cis-1893Ciona savignyi63.359e-138 401
LLPS-Cii-2088Ciona intestinalis62.147e-135 393
LLPS-Tru-0840Triticum urartu50.24e-80 253
LLPS-Drm-1779Drosophila melanogaster48.082e-95 292
LLPS-Tra-1317Triticum aestivum48.043e-84 264
LLPS-Asn-1594Aspergillus nidulans47.886e-73 237
LLPS-Ast-0998Aspergillus terreus47.886e-73 237
LLPS-Brd-1378Brachypodium distachyon47.694e-84 263
LLPS-Hov-0840Hordeum vulgare47.693e-84 264
LLPS-Sei-1626Setaria italica47.693e-83 261
LLPS-Zem-0778Zea mays47.693e-83 261
LLPS-Sob-0979Sorghum bicolor47.694e-83 261
LLPS-Tum-0438Tuber melanosporum47.652e-86 269
LLPS-Asc-1622Aspergillus clavatus47.462e-72 236
LLPS-Cus-1965Cucumis sativus47.331e-81 257
LLPS-Php-2039Physcomitrella patens47.332e-83 261
LLPS-Vir-2190Vigna radiata46.982e-81 257
LLPS-Via-0612Vigna angularis46.982e-81 257
LLPS-Mua-2350Musa acuminata46.983e-82 259
LLPS-Chr-1276Chlamydomonas reinhardtii46.812e-84 264
LLPS-Thc-0954Theobroma cacao46.813e-81 256
LLPS-Met-2190Medicago truncatula46.622e-81 257
LLPS-Dac-0370Daucus carota46.627e-81 255
LLPS-Glm-0528Glycine max46.625e-81 255
LLPS-Prp-1043Prunus persica46.624e-81 256
LLPS-Pot-2109Populus trichocarpa46.621e-81 257
LLPS-Fuv-0518Fusarium verticillioides46.262e-79 251
LLPS-Fuo-0767Fusarium oxysporum46.262e-79 251
LLPS-Phv-2503Phaseolus vulgaris46.262e-80 254
LLPS-Mae-0333Manihot esculenta46.266e-79 250
LLPS-Amt-2244Amborella trichopoda46.264e-80 253
LLPS-Sem-1147Selaginella moellendorffii46.17e-79 250
LLPS-Asfu-0226Aspergillus fumigatus45.988e-64 214
LLPS-Coo-0912Colletotrichum orbiculare45.917e-79 250
LLPS-Hea-2632Helianthus annuus45.912e-80 254
LLPS-Ori-1871Oryza indica45.912e-80 254
LLPS-Org-0989Oryza glaberrima45.912e-80 254
LLPS-Coc-2009Corchorus capsularis45.914e-79 251
LLPS-Orbr-0120Oryza brachyantha45.914e-80 253
LLPS-Ors-2240Oryza sativa45.912e-80 254
LLPS-Nef-0331Neosartorya fischeri45.811e-64 215
LLPS-Sac-0350Saccharomyces cerevisiae45.683e-82 258
LLPS-Asg-1364Ashbya gossypii45.686e-81 255
LLPS-Lep-0462Leersia perrieri45.616e-77 247
LLPS-Gag-0203Gaeumannomyces graminis45.619e-77 244
LLPS-Scj-1275Schizosaccharomyces japonicus45.492e-82 259
LLPS-Map-1172Magnaporthe poae45.266e-77 245
LLPS-Cogr-1117Colletotrichum graminicola45.24e-76 243
LLPS-Cog-1349Colletotrichum gloeosporioides45.24e-75 240
LLPS-Gor-2708Gossypium raimondii45.171e-78 250
LLPS-Brn-3031Brassica napus45.132e-80 253
LLPS-Bro-0951Brassica oleracea45.132e-80 253
LLPS-Ved-0821Verticillium dahliae45.03e-77 246
LLPS-Yal-0682Yarrowia lipolytica44.963e-79 250
LLPS-Nia-1959Nicotiana attenuata44.842e-78 249
LLPS-Asni-0738Aspergillus niger44.833e-78 248
LLPS-Arl-0856Arabidopsis lyrata44.772e-80 254
LLPS-Osl-0439Ostreococcus lucimarinus44.773e-78 249
LLPS-Kop-1512Komagataella pastoris44.62e-81 256
LLPS-Orni-1081Oryza nivara44.562e-76 243
LLPS-Orb-2410Oryza barthii44.562e-76 243
LLPS-Orr-0099Oryza rufipogon44.562e-76 243
LLPS-Orp-2229Oryza punctata44.562e-76 243
LLPS-Sol-2329Solanum lycopersicum44.482e-78 249
LLPS-Scc-1036Schizosaccharomyces cryophilus44.46e-81 255
LLPS-Art-2991Arabidopsis thaliana44.41e-79 252
LLPS-Trv-1362Trichoderma virens44.212e-74 239
LLPS-Pyt-1290Pyrenophora teres44.183e-75 241
LLPS-Pytr-0980Pyrenophora triticirepentis44.183e-75 241
LLPS-Fus-1620Fusarium solani43.778e-72 231
LLPS-Viv-2510Vitis vinifera43.779e-76 242
LLPS-Scp-1305Schizosaccharomyces pombe43.685e-81 255
LLPS-Zyt-1214Zymoseptoria tritici43.556e-72 233
LLPS-Nec-1263Neurospora crassa43.427e-77 245
LLPS-Blg-0800Blumeria graminis43.49e-75 239
LLPS-Orgl-1672Oryza glumaepatula43.337e-76 243
LLPS-Lem-1390Leptosphaeria maculans43.34e-74 238
LLPS-Aso-1144Aspergillus oryzae43.129e-70 226
LLPS-Dos-1497Dothistroma septosporum42.953e-72 234
LLPS-Mao-1508Magnaporthe oryzae42.819e-77 244
LLPS-Miv-0597Microbotryum violaceum42.165e-66 225
LLPS-Asf-0557Aspergillus flavus41.842e-48 173
LLPS-Trr-0626Trichoderma reesei41.641e-72 233
LLPS-Crn-0109Cryptococcus neoformans41.588e-68 222
LLPS-Phn-1163Phaeosphaeria nodorum40.491e-61 206
LLPS-Pug-0111Puccinia graminis38.961e-59 201
LLPS-Beb-0840Beauveria bassiana38.944e-68 223
LLPS-Brr-1883Brassica rapa38.672e-64 213
LLPS-Usm-1223Ustilago maydis38.563e-67 222
LLPS-Abg-1841Absidia glauca37.997e-56 192
LLPS-Mel-1053Melampsora laricipopulina37.669e-57 194
LLPS-Spr-0688Sporisorium reilianum36.929e-65 216
LLPS-Put-0361Puccinia triticina35.783e-55 191
LLPS-Cym-0283Cyanidioschyzon merolae31.331e-45 165
LLPS-Orm-0918Oryza meridionalis26.389e-1579.0
LLPS-Cae-1131Caenorhabditis elegans25.423e-1992.0
LLPS-Asm-2887Astyanax mexicanus25.145e-1270.9
LLPS-Sot-2109Solanum tuberosum23.463e-1477.4
LLPS-Gas-0912Galdieria sulphuraria23.143e-1477.4
LLPS-Chc-0561Chondrus crispus21.653e-1373.6