LLPS-Hos-2507
EIF4B
▼ Classification
Condensates:
| Condensate | Description | Tissue/Cell | PMIDs |
|---|---|---|---|
| Stress granule | "...We found that S. flexneri infection inhibits movement of the stress granule markers eIF3 and eIF4B into stress granules and prevents the aggregation of G3BP1 and eIF4G‐containing stress granules." | HeLa cells | 27282465 |
| Stress granule | "...A list of candidate SG proteins identified by mass spectrometry in NPCs, including statistical analysis." | Neural progenitor cells | 29373831 |
| Stress granule | "...283 proteins identified as APEX hits in HEK293T cells or previously shown to associate with SGs." | N/A | 29373831 |
| Stress granule | "...By adding known stress granule components not identified by mass spectrometry to this list, we created the first stress granule proteome for a mammalian cell." | U2OS cells | 26777405 |
| Stress granule | "...We performed immunoprecipitation and Nano-LC-MS/MS analysis to investigate the interactome of MAEL, and identified 14 components of stress granules as potential binding partners of MAEL in MDA-MB-231 human breast cancer and SW480 colorectal cancer cells. The interactions between MAEL and 8 of these SG components were confirmed by anti-tag immunoprecipitation. Immunofluorescence analysis showed that MAEL co-localizes with the SG marker PABPC1 in SGs during oxidative stress." | MDA-MB-231 cells, SW480 cells | 24189637 |
| Stress granule | "...Together, our APEX and IF screening approaches identified 260 SG proteins, including ~150 candidates that had not previously been associated with SGs." | Neural progenitor cells, HEK293T cells | 29373831 |
▼ FUNCTION
| Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F. |
▼ CROSS REFERENCE
| Database | Nucleotide ID | Protein ID |
|---|---|---|
| Ensembl | ENST00000550704.5 | ENSP00000448365.1 |
| Ensembl | ENST00000420463.7 | ENSP00000388806.3 |
| Ensembl | ENST00000416762.7 | ENSP00000412530.3 |
| Ensembl | ENST00000551002.5 | ENSP00000447192.1 |
| Ensembl | ENST00000552490.5 | ENSP00000450324.1 |
| Ensembl | ENST00000550390.1 | ENSP00000448980.1 |
| Ensembl | ENST00000549481.5 | ENSP00000449746.1 |
| Ensembl | ENST00000549077.5 | ENSP00000448399.1 |
| Ensembl | ENST00000262056.13 | ENSP00000262056.9 |
| UniProt | P23588, IF4B_HUMAN, B4DS13, Q4G0E3, Q53HQ2, Q6GPH5, Q6IB46, Q8WYK5 | |
| GeneBank | BC098437, BC073154, AK222528, CH471054, X55733, AK299526, AB076839, AC068888, CR456958, BC073139 | AAH73139.1, BAG61475.1, EAW96657.1, AAH73154.1, BAD96248.1, CAA39265.1, CAG33239.1, BAB82380.1, AAH98437.1 |
| RefSeq | NM_001330654.1, NM_001417.6 | NP_001317583.1, NP_001408.2 |
| Entrez | 1975 |
▼ SEQUENCE
| Protein Sequence (FASTA) |
|---|
1 MAASAKKKNK KGKTISLTDF LAEDGGTGGG STYVSKPVSW ADETDDLEGD VSTTWHSNDD 60 61 DVYRAPPIDR SILPTAPRAA REPNIDRSRL PKSPPYTAFL GNLPYDVTEE SIKEFFRGLN 120 121 ISAVRLPREP SNPERLKGFG YAEFEDLDSL LSALSLNEES LGNRRIRVDV ADQAQDKDRD 180 181 DRSFGRDRNR DSDKTDTDWR ARPATDSFDD YPPRRGDDSF GDKYRDRYDS DRYRDGYRDG 240 241 YRDGPRRDMD RYGGRDRYDD RGSRDYDRGY DSRIGSGRRA FGSGYRRDDD YRGGGDRYED 300 301 RYDRRDDRSW SSRDDYSRDD YRRDDRGPPQ RPKLNLKPRS TPKEDDSSAS TSQSTRAASI 360 361 FGGAKPVDTA AREREVEERL QKEQEKLQRQ LDEPKLERRP RERHPSWRSE ETQERERSRT 420 421 GSESSQTGTS TTSSRSKSDQ DARRRESEKS LENETLNKEE DCHSPTSKPP KPDQPLKVMP 480 481 APPPKENAWV KRSSNPPARS QSSDTEQQSP TSGGGKVAPA QPSEEGPGRK DENKVDGMNA 540 541 PKGQTGNSSR GPGDGGNRDH WKESDRKDGK KDQDSRSAPE PKKPEENPAS KFSSASKYAA 600 601 LSVDGEDENE GEDYAE 616 |
| Nucleotide CDS Sequence (FASTA) |
1 ATGGCGGCCT CAGCAAAAAA GAAGAATAAG AAGGGGAAGA CTATCTCCCT AACAGACTTT 60 61 CTGGCTGAGG ATGGGGGTAC TGGTGGAGGA AGCACCTATG TTTCCAAACC AGTCAGCTGG 120 121 GCTGATGAAA CGGATGACCT GGAAGGAGAT GTTTCGACCA CTTGGCACAG TAACGATGAC 180 181 GATGTGTATA GGGCGCCTCC AATTGACCGT TCCATCCTTC CCACTGCTCC ACGGGCTGCT 240 241 CGGGAACCCA ATATCGACCG GAGCCGTCTT CCCAAATCGC CACCCTACAC TGCTTTTCTA 300 301 GGAAACCTAC CCTATGATGT TACAGAAGAG TCAATTAAGG AATTCTTTCG AGGATTAAAT 360 361 ATCAGTGCAG TGCGTTTACC ACGTGAACCC AGCAATCCAG AGAGGTTGAA AGGTTTTGGT 420 421 TATGCTGAAT TTGAGGACCT GGATTCCCTG CTCAGTGCCC TGAGTCTCAA TGAAGAGTCT 480 481 CTAGGTAACA GGAGAATTCG AGTGGACGTT GCTGATCAAG CACAGGATAA AGACAGGGAT 540 541 GATCGTTCTT TTGGCCGTGA TAGAAATCGG GATTCTGACA AAACAGATAC AGACTGGAGG 600 601 GCTCGTCCTG CTACAGACAG CTTTGATGAC TACCCACCTA GAAGAGGTGA TGATAGCTTT 660 661 GGAGACAAGT ATCGAGATCG TTATGATTCA GACCGGTATC GGGATGGGTA TCGGGATGGG 720 721 TATCGGGATG GCCCACGCCG GGATATGGAT CGATATGGTG GCCGGGATCG CTATGATGAC 780 781 CGAGGCAGCA GAGACTATGA TAGAGGCTAT GATTCCCGGA TAGGCAGTGG CAGAAGAGCA 840 841 TTTGGCAGTG GGTATCGCAG GGATGATGAC TACAGAGGAG GCGGGGACCG CTATGAAGAC 900 901 CGATATGACA GACGGGATGA TCGGTCGTGG AGCTCCAGAG ATGATTACTC TCGGGATGAT 960 961 TATAGGCGTG ATGATAGAGG TCCCCCCCAA AGACCCAAAC TGAATCTAAA GCCTCGGAGT 1020 1021 ACTCCTAAGG AAGATGATTC CTCTGCTAGT ACCTCCCAGT CCACTCGAGC TGCTTCTATC 1080 1081 TTTGGAGGGG CAAAGCCTGT TGACACAGCT GCTAGAGAAA GAGAAGTAGA AGAACGGCTA 1140 1141 CAGAAGGAAC AAGAGAAGTT GCAGCGTCAG CTGGATGAGC CAAAACTAGA ACGACGGCCT 1200 1201 CGGGAGAGAC ACCCAAGCTG GCGAAGTGAA GAAACTCAGG AACGGGAACG GTCGAGGACA 1260 1261 GGAAGTGAGT CATCACAAAC TGGGACCTCC ACCACATCTA GCAGAAATGC ACGAAGGAGA 1320 1321 GAGAGTGAGA AGTCTCTAGA AAATGAAACA CTCAATAAGG AGGAAGATTG CCACTCTCCA 1380 1381 ACTTCTAAAC CTCCCAAACC TGATCAGCCC CTAAAGGTAA TGCCAGCCCC TCCACCAAAG 1440 1441 GAGAATGCTT GGGTGAAGCG AAGTTCTAAC CCTCCTGCTC GATCTCAGAG CTCAGACACA 1500 1501 GAGCAGCAGT CCCCTACAAG TGGTGGGGGA AAAGTAGCTC CAGCTCAACC ATCTGAGGAA 1560 1561 GGACCAGGAA GGAAAGATGA AAATAAAGTA GATGGGATGA ATGCCCCAAA AGGCCAAACT 1620 1621 GGGAACTCTA GCCGTGGTCC AGGCGACGGA GGGAACAGAG ACCACTGGAA GGAGTCAGAT 1680 1681 AGGAAAGATG GCAAAAAGGA TCAAGACTCC AGATCTGCAC CTGAGCCAAA GAAACCTGAG 1740 1741 GAAAATCCAG CTTCCAAGTT CAGTTCTGCA AGCAAGTATG CTGCTCTCTC TGTTGATGGT 1800 1801 GAAGATGAAA ATGAGGGAGA AGATTATGCC GAATAG 1836 |
▼ KEYWORD
ID | Family |
3D-structure | |
Acetylation | |
Alternative splicing | |
Complete proteome | |
Direct protein sequencing | |
Initiation factor | |
Isopeptide bond | |
Phosphoprotein | |
Polymorphism | |
Protein biosynthesis | |
Reference proteome | |
RNA-binding | |
Ubl conjugation
|
▼ GENE ONTOLOGY
ID | Classification | Description |
Cellular Component | Cytosol | |
Cellular Component | Eukaryotic translation initiation factor 4F complex | |
Cellular Component | Polysome | |
Molecular Function | Ribosomal small subunit binding | |
Molecular Function | RNA binding | |
Molecular Function | RNA strand annealing activity | |
Molecular Function | RNA strand-exchange activity | |
Molecular Function | Translation initiation factor activity | |
Biological Process | Cytoplasmic translation | |
Biological Process | Eukaryotic translation initiation factor 4F complex assembly | |
Biological Process | Formation of translation preinitiation complex | |
Biological Process | Regulation of translational initiation | |
Biological Process | Translational initiation |
▼ ANNOTATION
| Drug | ||||
| CTD | ||||
| Physicochemical | ||||
| Compute pI/Mw | AAindex | |||
| Function | ||||
| ENPD | CellMarker | RaftProt | ||
| Localization | ||||
| COMPARTMENTS | ||||
| Methylation | ||||
| TCGA | ICGC | MethyCancer | ||
▼ ORTHOLOGY
| DrLLPS ID | Organism | Identity | E-value | Score |
|---|---|---|---|---|
| LLPS-Rhb-1587 | Rhinopithecus bieti | 100.0 | 2e-110 | 352 |
| LLPS-Mam-1198 | Macaca mulatta | 100.0 | 1e-110 | 352 |
| LLPS-Poa-4226 | Pongo abelii | 100.0 | 1e-110 | 352 |
| LLPS-Nol-1862 | Nomascus leucogenys | 100.0 | 1e-110 | 352 |
| LLPS-Aon-4015 | Aotus nancymaae | 100.0 | 2e-110 | 351 |
| LLPS-Cea-1619 | Cercocebus atys | 100.0 | 6e-109 | 347 |
| LLPS-Chs-2714 | Chlorocebus sabaeus | 100.0 | 1e-110 | 352 |
| LLPS-Maf-0348 | Macaca fascicularis | 100.0 | 2e-110 | 352 |
| LLPS-Caj-4149 | Callithrix jacchus | 100.0 | 2e-110 | 352 |
| LLPS-Mal-2552 | Mandrillus leucophaeus | 100.0 | 9e-111 | 351 |
| LLPS-Paa-3037 | Papio anubis | 100.0 | 2e-110 | 352 |
| LLPS-Pat-1894 | Pan troglodytes | 99.65 | 1e-117 | 370 |
| LLPS-Urm-1727 | Ursus maritimus | 99.55 | 2e-109 | 348 |
| LLPS-Gog-2104 | Gorilla gorilla | 99.55 | 4e-110 | 350 |
| LLPS-Aim-3094 | Ailuropoda melanoleuca | 99.52 | 3e-107 | 343 |
| LLPS-Mup-0336 | Mustela putorius furo | 99.52 | 7e-107 | 342 |
| LLPS-Fec-2920 | Felis catus | 99.09 | 8e-109 | 347 |
| LLPS-Eqc-1556 | Equus caballus | 99.09 | 6e-109 | 348 |
| LLPS-Caf-3327 | Canis familiaris | 99.09 | 1e-108 | 347 |
| LLPS-Sus-1010 | Sus scrofa | 99.09 | 2e-108 | 347 |
| LLPS-Orc-0690 | Oryctolagus cuniculus | 99.03 | 1e-106 | 342 |
| LLPS-Cas-1831 | Carlito syrichta | 98.64 | 2e-108 | 346 |
| LLPS-Bot-3080 | Bos taurus | 98.64 | 3e-108 | 345 |
| LLPS-Ova-2205 | Ovis aries | 98.64 | 3e-108 | 346 |
| LLPS-Loa-3829 | Loxodonta africana | 98.55 | 1e-105 | 339 |
| LLPS-Mea-0844 | Mesocricetus auratus | 98.55 | 3e-106 | 340 |
| LLPS-Fud-3286 | Fukomys damarensis | 98.55 | 3e-99 | 322 |
| LLPS-Tut-0319 | Tursiops truncatus | 98.25 | 6e-21 | 92.8 |
| LLPS-Ran-0022 | Rattus norvegicus | 98.18 | 1e-107 | 343 |
| LLPS-Cap-2169 | Cavia porcellus | 97.73 | 3e-100 | 325 |
| LLPS-Mum-3714 | Mus musculus | 97.73 | 6e-107 | 342 |
| LLPS-Myl-0390 | Myotis lucifugus | 97.58 | 6e-106 | 339 |
| LLPS-Man-2324 | Macaca nemestrina | 97.27 | 6e-107 | 342 |
| LLPS-Dio-0700 | Dipodomys ordii | 96.62 | 4e-103 | 332 |
| LLPS-Sah-0622 | Sarcophilus harrisii | 95.07 | 3e-90 | 298 |
| LLPS-Ora-0740 | Ornithorhynchus anatinus | 93.33 | 3e-28 | 117 |
| LLPS-Ict-0715 | Ictidomys tridecemlineatus | 93.18 | 3e-93 | 305 |
| LLPS-Otg-3827 | Otolemur garnettii | 92.18 | 3e-74 | 256 |
| LLPS-Pes-0945 | Pelodiscus sinensis | 91.87 | 9e-100 | 317 |
| LLPS-Anc-0048 | Anolis carolinensis | 90.2 | 2e-90 | 299 |
| LLPS-Fia-3662 | Ficedula albicollis | 86.67 | 4e-87 | 290 |
| LLPS-Meg-0545 | Meleagris gallopavo | 86.26 | 8e-70 | 229 |
| LLPS-Pap-1817 | Pan paniscus | 86.01 | 6e-81 | 271 |
| LLPS-Tag-0594 | Taeniopygia guttata | 85.71 | 3e-84 | 279 |
| LLPS-Xet-1623 | Xenopus tropicalis | 84.32 | 1e-88 | 294 |
| LLPS-Gaga-0653 | Gallus gallus | 83.96 | 5e-82 | 282 |
| LLPS-Lac-0230 | Latimeria chalumnae | 82.21 | 6e-80 | 272 |
| LLPS-Leo-0282 | Lepisosteus oculatus | 78.4 | 8e-72 | 249 |
| LLPS-Asm-0411 | Astyanax mexicanus | 77.39 | 6e-75 | 258 |
| LLPS-Scf-2829 | Scleropages formosus | 77.03 | 1e-70 | 244 |
| LLPS-Icp-3030 | Ictalurus punctatus | 76.55 | 2e-70 | 245 |
| LLPS-Pof-0406 | Poecilia formosa | 75.77 | 2e-71 | 248 |
| LLPS-Xim-0910 | Xiphophorus maculatus | 75.77 | 2e-71 | 248 |
| LLPS-Gaa-2224 | Gasterosteus aculeatus | 75.23 | 1e-68 | 241 |
| LLPS-Dar-2651 | Danio rerio | 74.77 | 1e-71 | 248 |
| LLPS-Orn-1563 | Oreochromis niloticus | 73.36 | 1e-67 | 238 |
| LLPS-Ten-3352 | Tetraodon nigroviridis | 72.07 | 3e-65 | 231 |
| LLPS-Ere-0440 | Erinaceus europaeus | 71.43 | 2e-09 | 60.1 |
| LLPS-Tar-3311 | Takifugu rubripes | 67.18 | 1e-52 | 187 |
| LLPS-Orl-0417 | Oryzias latipes | 67.09 | 9e-60 | 216 |
| LLPS-Scm-0188 | Scophthalmus maximus | 66.52 | 5e-62 | 223 |
| LLPS-Cogr-0744 | Colletotrichum graminicola | 55.56 | 2e-09 | 64.3 |
| LLPS-Cii-0535 | Ciona intestinalis | 42.31 | 6e-17 | 86.3 |
| LLPS-Cis-0418 | Ciona savignyi | 41.86 | 9e-18 | 90.9 |
| LLPS-Trr-1231 | Trichoderma reesei | 36.03 | 5e-12 | 72.8 |
| LLPS-Mao-0363 | Magnaporthe oryzae | 35.25 | 1e-09 | 65.5 |
| LLPS-Beb-0582 | Beauveria bassiana | 34.72 | 3e-09 | 63.9 |
| LLPS-Tum-0598 | Tuber melanosporum | 34.62 | 3e-06 | 54.3 |
| LLPS-Fuo-1020 | Fusarium oxysporum | 34.03 | 8e-08 | 59.3 |
| LLPS-Fuv-0811 | Fusarium verticillioides | 34.03 | 9e-08 | 59.3 |
| LLPS-Spr-0043 | Sporisorium reilianum | 33.33 | 7e-06 | 53.1 |
| LLPS-Fus-1011 | Fusarium solani | 32.87 | 1e-09 | 65.1 |
| LLPS-Usm-1025 | Ustilago maydis | 32.37 | 9e-06 | 52.8 |